Executing align2.BBMap [build=1, overwrite=true, match=long, fastareadlen=500, ref=aclum.TNNW/spades/scaffolds.fasta, in=/global/projectb/scratch/aclum/gaa-691/TNNW/phixscreen_jigsaw/spades/corrected/TNNW.7707.1.80834.CCGTC.nophix.contam.artifact.clean_1.fastq.00.cor.fastq.gz, in2=/global/projectb/scratch/aclum/gaa-691/TNNW/phixscreen_jigsaw/spades/corrected/TNNW.7707.1.80834.CCGTC.nophix.contam.artifact.clean_2.fastq.00.cor.fastq.gz, out=TNNW.spades.assembled.reads] BBMap version 31.26 Set OVERWRITE to true Cigar strings enabled. Retaining first best site only for ambiguous mappings. Writing reference. Set genome to 1 Loaded Reference: 0.169 seconds. Generated Index: 3.717 seconds. Analyzed Index: 2.592 seconds. Started output stream: 3.634 seconds. Cleared Memory: 0.380 seconds. Processing reads in paired-ended mode. Started read stream. Started 32 mapping threads. Detecting finished threads: 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31 ------------------ Results ------------------ Genome: 1 Key Length: 13 Max Indel: 16000 Minimum Score Ratio: 0.56 Mapping Mode: normal Reads Used: 5821332 (1444859929 bases) Mapping: 347.361 seconds. Reads/sec: 8379.37 kBases/sec: 4159.53 Read 1 data: mapped: 80.7900% 2351526 reads unambiguous: 75.1913% 2188567 reads Mated pairs: 21.6109% bad pairs: 46.710% (of all reads) rescued: 3.579% avg insert size: 370.83 perfect best site: 12.4676% semiperfect site: 48.6671% ambiguousMapping: 5.5987% (Kept) low-Q discards: 0.0000% Match Rate: 91.9817% 541438788 Error Rate: 1.0515% 6189617 Sub Rate: 0.8630% 5079655 Del Rate: 0.0655% 385624 Ins Rate: 0.1231% 724338 N Rate: 6.9668% 41009298 Read 2 data: mapped: 81.8837% 2383361 reads unambiguous: 75.1152% 2186353 reads rescued: 3.480% perfect best site: 10.8262% semiperfect site: 42.0086% ambiguousMapping: 6.7685% (Kept) low-Q discards: 0.0000% Match Rate: 92.0471% 539588840 Error Rate: 1.3109% 7684686 Sub Rate: 1.1125% 6521757 Del Rate: 0.0729% 427257 Ins Rate: 0.1255% 735672 N Rate: 6.6420% 38936052 Total time: 359.313 seconds.