Executing align2.BBMap [build=1, overwrite=true, match=long, fastareadlen=500, in=phixscreen_jigsaw_norm/spades/corrected/TNOC.7707.1.80834.CGTAC.nophix.contam.artifact.clean.norm.paired_1.fastq.00.cor.fastq.gz, in2=phixscreen_jigsaw_norm/spades/corrected/TNOC.7707.1.80834.CGTAC.nophix.contam.artifact.clean.norm.paired_2.fastq.00.cor.fastq.gz, ref=phixscreen_jigsaw_norm/spades/scaffolds.fasta] BBMap version 31.26 Set OVERWRITE to true Cigar strings enabled. Retaining first best site only for ambiguous mappings. No output file. Writing reference. Set genome to 1 Loaded Reference: 0.015 seconds. Generated Index: 2.416 seconds. Analyzed Index: 2.573 seconds. Cleared Memory: 1.034 seconds. Processing reads in paired-ended mode. Started read stream. Started 4 mapping threads. Detecting finished threads: 0, 1, 2, 3 ------------------ Results ------------------ Genome: 1 Key Length: 13 Max Indel: 16000 Minimum Score Ratio: 0.56 Mapping Mode: normal Reads Used: 14974 (3697653 bases) Mapping: 5.640 seconds. Reads/sec: 1327.58 kBases/sec: 655.66 Read 1 data: mapped: 99.4524% 7446 reads unambiguous: 78.9368% 5910 reads Mated pairs: 93.0546% bad pairs: 5.770% (of all reads) rescued: 0.080% avg insert size: 470.33 perfect best site: 80.6064% semiperfect site: 81.7150% ambiguousMapping: 20.5156% (Kept) low-Q discards: 0.0000% Match Rate: 81.9021% 1839834 Error Rate: 17.9141% 402418 Sub Rate: 0.2786% 6258 Del Rate: 17.3694% 390184 Ins Rate: 0.2660% 5976 N Rate: 0.1838% 4129 Read 2 data: mapped: 99.2387% 7430 reads unambiguous: 78.7765% 5898 reads rescued: 0.120% perfect best site: 69.3469% semiperfect site: 70.5490% ambiguousMapping: 20.4621% (Kept) low-Q discards: 0.0000% Match Rate: 77.8301% 1796906 Error Rate: 21.9446% 506647 Sub Rate: 0.4696% 10841 Del Rate: 21.2002% 489460 Ins Rate: 0.2749% 6346 N Rate: 0.2253% 5201 Total time: 11.855 seconds.