Executing align2.BBMap [build=1, overwrite=true, match=long, fastareadlen=500, ref=aclum.TNOH/spades/scaffolds.fasta, in=aclum.TNOH/spades/corrected/TNOH.7707.1.80834.GGTAG.nophix.contam.artifact.clean_1.fastq.00.cor.fastq.gz, in2=aclum.TNOH/spades/corrected/TNOH.7707.1.80834.GGTAG.nophix.contam.artifact.clean_2.fastq.00.cor.fastq.gz] BBMap version 31.26 Set OVERWRITE to true Cigar strings enabled. Retaining first best site only for ambiguous mappings. No output file. Writing reference. Set genome to 1 Loaded Reference: 0.204 seconds. Generated Index: 4.718 seconds. Analyzed Index: 3.030 seconds. Cleared Memory: 0.561 seconds. Processing reads in paired-ended mode. Started read stream. Started 8 mapping threads. Detecting finished threads: 0, 1, 2, 3, 4, 5, 6, 7 ------------------ Results ------------------ Genome: 1 Key Length: 13 Max Indel: 16000 Minimum Score Ratio: 0.56 Mapping Mode: normal Reads Used: 6341616 (1554598833 bases) Mapping: 6051.486 seconds. Reads/sec: 523.97 kBases/sec: 256.90 Read 1 data: mapped: 98.9402% 3137204 reads unambiguous: 80.0404% 2537928 reads Mated pairs: 58.6827% bad pairs: 39.143% (of all reads) rescued: 2.786% avg insert size: 527.24 perfect best site: 37.3204% semiperfect site: 58.7257% ambiguousMapping: 18.8998% (Kept) low-Q discards: 0.0000% Match Rate: 24.3643% 722038568 Error Rate: 74.0435% 2194284691 Sub Rate: 0.3783% 11210862 Del Rate: 73.0937% 2166138332 Ins Rate: 0.5715% 16935497 N Rate: 1.5922% 47185487 Read 2 data: mapped: 98.8319% 3133771 reads unambiguous: 79.1614% 2510056 reads rescued: 2.975% perfect best site: 30.9901% semiperfect site: 49.2505% ambiguousMapping: 19.6705% (Kept) low-Q discards: 0.0000% Match Rate: 25.0796% 697146033 Error Rate: 73.3613% 2039251904 Sub Rate: 0.4636% 12885497 Del Rate: 72.2622% 2008699490 Ins Rate: 0.6356% 17666917 N Rate: 1.5592% 43340584 Total time: 6063.019 seconds.