QD/SAG JGI SINGLE-CELL QC AND ASSEMBLY REPORT - 1) RAW DATA: LibraryName NumReads RunType ReadType FileName LIB 100110 2x150 Illumina Std PE (cassava 1.8) ZHZB.15M.bbqc.fq.gz.fastq a) SPAdes assembly: Assembly stats of the SPAdes assembly. Estimated Genome Recovery using Single-copy Gene Analysis: archaea HMM: 0% bacteria HMM: 0% Avg GC Content: 39.41 +/- 1.79% Largest Contig: 19.5 KB Main genome scaffold total: 7 Main genome contig total: 8 Main genome scaffold sequence total: 60.2 KB Main genome contig sequence total: 60.2 KB (-> 0.0% gap) Main genome scaffold N/L50: 2/16.9 KB Main genome contig N/L50: 2/13.6 KB Number of scaffolds > 50 KB: 0 % main genome in scaffolds > 50 KB: 0.0% Minimum Number Number Total Total Scaffold Scaffold of of Scaffold Contig Contig Length Scaffolds Contigs Length Length Coverage -------- --------- ------- ----------- ----------- -------- All 7 8 60,187 60,167 99.97% 1 kb 7 8 60,187 60,167 99.97% 2.5 kb 5 6 55,820 55,800 99.96% 5 kb 4 5 52,845 52,825 99.96% 10 kb 3 4 46,639 46,619 99.96% 2) NCBI SCREENING STATS This step identifies potential contaminants screened by NCBI for submission. ** No potential contaminants found. 3) KEY PIPELINE CMDS: a) SPAdes assembly step: SPAdes version: 3.0.0 SPAdes params: -t 16 -m 120 --sc --careful --12 4) WORKFLOW STEPS: 1. Created SPAdes assembly. 5) ASSEMBLERS USED: SPAdes (v.3.0.0) 6) ASSESSED GENOME PROJECT STANDARD: Standard Draft 7) RELEASE DATE: 03/28/2015 8) AUTHORS: For additional information, please contact: NAME_OF_ANALYST - ANALYST@lbl.gov Stephan Trong - strong@lbl.gov Alex Copeland - accopeland@lbl.gov This file was automatically generated by the jigsaw pipeline software (version 2.4.2).