QD/SAG JGI SINGLE-CELL QC AND ASSEMBLY REPORT - 1) RAW DATA: LibraryName NumReads RunType ReadType FileName LIB 30227690 2x149 Illumina Std PE (cassava 1.8) ZHZG.bbqc.fq.gz.fastq a) SPAdes assembly: Assembly stats of the SPAdes assembly. Estimated Genome Recovery using Single-copy Gene Analysis: archaea HMM: 2.58% bacteria HMM: 1.48% Avg GC Content: 40.71 +/- 9.39% Largest Contig: 33.3 KB Main genome scaffold total: 54 Main genome contig total: 55 Main genome scaffold sequence total: 292.6 KB Main genome contig sequence total: 292.5 KB (-> 0.0% gap) Main genome scaffold N/L50: 11/6.2 KB Main genome contig N/L50: 11/6.2 KB Number of scaffolds > 50 KB: 0 % main genome in scaffolds > 50 KB: 0.0% Minimum Number Number Total Total Scaffold Scaffold of of Scaffold Contig Contig Length Scaffolds Contigs Length Length Coverage -------- --------- ------- ----------- ----------- -------- All 54 55 292,557 292,516 99.99% 1 kb 54 55 292,557 292,516 99.99% 2.5 kb 45 46 273,086 273,045 99.98% 5 kb 16 17 177,771 177,730 99.98% 10 kb 4 4 97,704 97,704 100.00% 25 kb 2 2 63,613 63,613 100.00% 2) NCBI SCREENING STATS This step identifies potential contaminants screened by NCBI for submission. ** No potential contaminants found. 3) KEY PIPELINE CMDS: a) SPAdes assembly step: SPAdes version: 3.0.0 SPAdes params: -t 8 -m 40 --sc --careful --12 4) WORKFLOW STEPS: 1. Created SPAdes assembly. 5) ASSEMBLERS USED: SPAdes (v.3.0.0) 6) ASSESSED GENOME PROJECT STANDARD: Standard Draft 7) RELEASE DATE: 03/25/2015 8) AUTHORS: For additional information, please contact: NAME_OF_ANALYST - ANALYST@lbl.gov Stephan Trong - strong@lbl.gov Alex Copeland - accopeland@lbl.gov This file was automatically generated by the jigsaw pipeline software (version 2.4.2).