Pool name = M3388 Library names = M3380, M3381, M3382, M3383, M3384, M3385, M3386, M3387. Run folder= 140214_M02100_0021_000000000-A659B. #Sample Id Miseq Name Sample name 54320 M3380 E98 54314 M3381 A87 54315 M3382 B105 54316 M3383 B108 54317 M3384 C184 54318 M3385 D70 54148 M3386 A74 54319 M3387 E96 #Seq Unit Individual Library Name Index Name Index Sequence Read Count Read % number of cosmids average reads per cosmid average coverage per cosmid 7737.1.81418.ATGTC.fastq.gz M3380 None ATGTC 6,287,968 14.84% 6000 1047.994667 6.986631111 7737.1.81418.CCGTC.fastq.gz M3381 None CCGTC 3,594,840 8.48% 6000 599.14 3.994266667 7737.1.81418.GTAGA.fastq.gz M3382 None GTAGA 3,373,328 7.96% 6000 562.2213333 3.748142222 7737.1.81418.GTCCG.fastq.gz M3383 None GTCCG 8,177,986 19.29% 6000 1362.997667 9.086651111 7737.1.81418.GTGAA.fastq.gz M3384 None GTGAA 6,082,056 14.35% 6000 1013.676 6.75784 7737.1.81418.GTGGC.fastq.gz M3385 None GTGGC 3,594,834 8.48% 6000 599.139 3.99426 7737.1.81418.GTTTC.fastq.gz M3386 None GTTTC 4,289,640 10.12% 7737.1.81418.CGTAC.fastq.gz M3387 None CGTAC 4,341,138 10.24% 6000 723.523 4.823486667 7737.1.81418.UNKNOWN.fastq.gz UNKNOWN None UNKNOWN 2,642,590 6.23 % Actual Total: 42,384,380 100.00 % Index distribution looks reasonable, 3.5 -8.1 million reads per index. Quality is ok, read 2 quality drops off more quickly than read 1. Assuming 6,000 cosmids per sample with an average size of 45 kb there are only 600-1,362 reads per cosmid or ~4-9x coverage so depending on what they want to do with the data additional sequence may be needed. # Post QC # ID reads M3380 /global/dna/shared/rqc/filtered_seq_unit/00/00/77/37/7737.1.81418.ATGTC.anqd.fastq.gz M3381 /global/dna/shared/rqc/filtered_seq_unit/00/00/77/37/7737.1.81418.CCGTC.anqd.fastq.gz M3382 /global/dna/shared/rqc/filtered_seq_unit/00/00/77/37/7737.1.81418.GTAGA.anqd.fastq.gz M3383 /global/dna/shared/rqc/filtered_seq_unit/00/00/77/37/7737.1.81418.GTCCG.anqd.fastq.gz M3384 /global/dna/shared/rqc/filtered_seq_unit/00/00/77/37/7737.1.81418.GTGAA.anqd.fastq.gz M3385 /global/dna/shared/rqc/filtered_seq_unit/00/00/77/37/7737.1.81418.GTGGC.anqd.fastq.gz M3386 /global/dna/shared/rqc/filtered_seq_unit/00/00/77/37/7737.1.81418.GTTTC.anqd.fastq.gz M3387 /global/dna/shared/rqc/filtered_seq_unit/00/00/77/37/7737.1.81418.CGTAC.anqd.fastq.gz # ID assembly M3380 /global/dna/shared/rqc/pipelines/assembly_qc/archive/02/82/45/16/7737.1.81418.ATGTC.subsampled5000000.fastq.velvet.k63/contigs.fa M3381 /global/dna/shared/rqc/pipelines/assembly_qc/archive/02/82/45/15/7737.1.81418.CCGTC.subsampled5000000.fastq.velvet.k63/contigs.fa M3382 /global/dna/shared/rqc/pipelines/assembly_qc/archive/02/82/45/20/7737.1.81418.GTAGA.subsampled5000000.fastq.velvet.k63/contigs.fa M3383 /global/dna/shared/rqc/pipelines/assembly_qc/archive/02/82/45/18/7737.1.81418.GTCCG.subsampled5000000.fastq.velvet.k63/contigs.fa M3384 /global/dna/shared/rqc/pipelines/assembly_qc/archive/02/82/45/19/7737.1.81418.GTGAA.subsampled5000000.fastq.velvet.k63/contigs.fa M3385 /global/dna/shared/rqc/pipelines/assembly_qc/archive/02/82/45/14/7737.1.81418.GTGGC.subsampled5000000.fastq.velvet.k63/contigs.fa M3386 /global/dna/shared/rqc/pipelines/assembly_qc/archive/02/82/45/17/7737.1.81418.GTTTC.subsampled5000000.fastq.velvet.k63/contigs.fa M3387 /global/dna/shared/rqc/pipelines/assembly_qc/archive/02/82/45/21/7737.1.81418.CGTAC.subsampled5000000.fastq.velvet.k63/contigs.fa