##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	bin_W-DeltaSymb.fastq.keep	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5136427	
Filtered Sequences	0	
Sequence length	150	
%GC	50	
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.093128160879147	33.0	31.0	34.0	27.0	34.0
2	31.181918676153675	33.0	31.0	34.0	26.0	34.0
3	31.19780695802744	33.0	31.0	34.0	26.0	34.0
4	34.51176703182971	37.0	35.0	37.0	30.0	37.0
5	34.51531307658028	37.0	35.0	37.0	30.0	37.0
6	34.38984998716033	37.0	35.0	37.0	30.0	37.0
7	34.38730872647465	37.0	35.0	37.0	30.0	37.0
8	34.392110897322205	37.0	35.0	37.0	30.0	37.0
9	35.86194703049416	39.0	35.0	39.0	30.0	39.0
10-14	36.0545930468787	39.2	35.2	39.4	29.4	39.4
15-19	36.82814002807788	40.0	36.0	41.0	27.8	41.0
20-24	34.55618222550423	38.8	32.0	40.8	20.0	41.0
25-29	29.9613076171432	35.4	23.4	40.0	8.4	41.0
30-34	28.57470440054926	34.6	17.4	40.0	4.4	41.0
35-39	27.282628916949466	33.2	14.4	40.0	3.0	41.0
40-44	25.968317859866396	32.0	9.4	39.0	3.0	41.0
45-49	24.662052161940586	30.6	7.6	37.8	3.0	41.0
50-54	23.03416659090064	28.4	4.6	36.2	3.0	40.0
55-59	21.944591911848452	26.6	3.0	35.0	3.0	39.2
60-64	20.847486667288372	25.4	3.0	35.0	3.0	37.4
65-69	19.754620556273846	22.8	3.0	35.0	3.0	35.8
70-74	18.795196933588272	20.0	3.0	34.0	3.0	35.0
75-79	18.003540554552806	18.0	3.0	33.8	3.0	35.0
80-84	17.325615374266977	12.2	3.0	33.6	3.0	35.0
85-89	16.688392767968864	9.2	3.0	33.0	3.0	35.0
90-94	16.17915551024087	7.0	3.0	33.0	3.0	35.0
95-99	15.66568336316276	7.0	3.0	32.0	3.0	35.0
100-104	15.548521803191207	7.0	3.0	32.0	3.0	35.0
105-109	16.54771256361669	5.0	3.0	33.0	3.0	35.0
110-114	16.293025248874365	3.0	3.0	33.0	3.0	35.0
115-119	16.055394031687786	3.0	3.0	33.0	3.0	35.0
120-124	15.408216762352506	3.0	3.0	32.4	3.0	35.0
125-129	14.679740605677836	3.0	3.0	31.2	3.0	34.4
130-134	13.971493958738245	3.0	3.0	30.8	3.0	34.0
135-139	13.243088785258701	3.0	3.0	29.6	3.0	34.0
140-144	12.199919632849838	3.0	3.0	28.2	3.0	34.0
145-149	10.67497515296139	3.0	3.0	19.4	3.0	33.2
150	8.490603682287318	3.0	3.0	3.0	3.0	29.0
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
5	9566.0
6	105337.0
7	192403.0
8	303438.0
9	235099.0
10	202219.0
11	181085.0
12	166581.0
13	156846.0
14	152297.0
15	148086.0
16	142714.0
17	136017.0
18	128006.0
19	122848.0
20	129167.0
21	165945.0
22	200757.0
23	206161.0
24	185910.0
25	151882.0
26	120398.0
27	100031.0
28	92528.0
29	94648.0
30	104060.0
31	118873.0
32	143728.0
33	182533.0
34	238769.0
35	286871.0
36	198004.0
37	33443.0
38	177.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.96651641306301	29.406277943792446	24.23920363318704	28.388002009957507
2	28.438718198467534	29.914899987870946	23.20827298820756	18.43810882545396
3	23.210492429854447	26.910321124003122	25.769119272988796	24.110067173153634
4	23.165246970316137	24.396725583756957	26.17422577990498	26.263801666021923
5	25.162608171010703	24.925965072607863	26.59009852568721	23.321328230694217
6	24.61008401365385	25.51363038937378	25.181473425009255	24.694812171963118
7	26.33455123571307	24.87415863206077	25.706488187216518	23.084801945009634
8	23.73581869264374	25.255746845034494	26.74779569533452	24.260638766987245
9	24.175774327173343	24.309602764723415	27.172487801345174	24.342135106758064
10-14	24.983117641893866	25.215166106711923	25.50294202565324	24.298774225740967
15-19	24.917005537117536	24.95589638478265	25.617519727234516	24.5095783508653
20-24	25.406777902226587	24.788351902986257	25.355742425619987	24.449127769167166
25-29	25.856643149021686	24.809611817709083	25.08296136594563	24.25078366732361
30-34	26.048021319099835	24.673544469725744	25.022467952917467	24.255966258256954
35-39	26.14975740918736	24.6479157593401	24.925696403355875	24.276630428116665
40-44	26.222866595787302	24.61243194929082	24.838908447448002	24.325793007473873
45-49	26.300605459787512	24.547530803027083	24.744087670281306	24.407776066904095
50-54	26.14709018545382	24.635253260681015	24.850126362157976	24.367530191707193
55-59	26.15803553715452	24.629572268816435	24.827783982912635	24.384608211116404
60-64	26.070764755344523	24.652405261478457	24.81700995653204	24.459820026644984
65-69	26.127189192020055	24.642962900085994	24.786973512910823	24.44287439498313
70-74	26.13544395744357	24.610177463828457	24.72249289243281	24.531885686295162
75-79	26.060099754167638	24.5923674180515	24.74419280172774	24.60334002605313
80-84	25.949158821881436	24.637667390191666	24.795247747120712	24.617926040806186
85-89	25.857453050534936	24.72635939340713	24.842895654897852	24.573291901160086
90-94	25.76107477045814	24.73042058224521	24.869139578932984	24.63936506836367
95-99	25.756133592475862	24.70182482881583	24.81216612248164	24.729875456226672
100-104	25.690488738572554	24.65300100634157	24.753389856411857	24.90312039867402
105-109	25.56410127117547	24.554267003113253	24.761492765301636	25.120138960409637
110-114	25.552112392525	24.557000420720474	24.77508587194951	25.11580131480502
115-119	25.542537643385177	24.488999843665646	24.744473152251555	25.22398936069762
120-124	25.514880285459135	24.404847961433113	24.74013161288966	25.340140140218093
125-129	25.499044374620723	24.35575546970686	24.682792143254446	25.46240801241797
130-134	25.509674331982136	24.284643780589114	24.647152582914153	25.5585293045146
135-139	25.472356562256216	24.130338073528545	24.65685582604406	25.740449538171184
140-144	25.52421362164789	24.02881224633388	24.58803755996143	25.858936572056802
145-149	25.50753276548075	23.94611662932229	24.483926277936003	26.062424327260953
150	26.35997747072041	22.709482681249046	24.260288328832473	26.670251519198075
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	46.35451842302052
2	46.87682702392149
3	47.320559603008086
4	49.42904863633807
5	48.483936401704916
6	49.30489618561696
7	49.41935318072271
8	47.99645745963099
9	48.51790943393141
10-14	49.28189186763483
15-19	49.42658388798284
20-24	49.85590567139375
25-29	50.10742681634529
30-34	50.30398757735679
35-39	50.42638783730402
40-44	50.54865960326118
45-49	50.7083815266916
50-54	50.51462037716101
55-59	50.54264374827093
60-64	50.5305847819895
65-69	50.570063587003176
70-74	50.66732964373873
75-79	50.66343978022076
80-84	50.56708486268763
85-89	50.43074495169502
90-94	50.40043983882181
95-99	50.48600904870253
100-104	50.593609137246574
105-109	50.68424023158511
110-114	50.667913707330015
115-119	50.766527004082796
120-124	50.855020425677225
125-129	50.9614523870387
130-134	51.06820363649673
135-139	51.2128061004274
140-144	51.38315019370469
145-149	51.5699570927417
150	53.030228989918484
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	15.0
2	15.0
3	21.5
4	27.0
5	34.0
6	68.0
7	91.5
8	110.5
9	155.5
10	209.5
11	261.0
12	333.5
13	419.0
14	512.5
15	615.5
16	748.0
17	902.0
18	1131.0
19	1474.5
20	1868.0
21	2359.5
22	2981.5
23	3851.5
24	5095.5
25	6744.5
26	8907.5
27	11581.0
28	14760.0
29	18614.5
30	23330.0
31	28643.5
32	34571.5
33	41437.5
34	48638.0
35	56539.5
36	65287.0
37	74556.5
38	84375.0
39	94486.0
40	104719.0
41	115236.0
42	126767.5
43	138310.5
44	150296.5
45	163168.0
46	175571.0
47	186755.5
48	197284.5
49	207345.0
50	214982.0
51	220104.5
52	222746.5
53	222654.0
54	220505.5
55	215365.0
56	206736.0
57	195443.0
58	181808.0
59	165866.0
60	148548.0
61	131140.0
62	113689.5
63	97120.0
64	81572.0
65	66875.5
66	53868.0
67	42694.5
68	33323.5
69	25597.5
70	19597.5
71	14904.0
72	11065.5
73	8052.5
74	5822.0
75	4156.5
76	2911.5
77	2028.5
78	1479.0
79	1096.0
80	803.5
81	629.5
82	487.0
83	383.5
84	298.0
85	221.0
86	157.5
87	120.5
88	101.5
89	71.5
90	51.0
91	39.5
92	29.5
93	21.0
94	14.5
95	9.5
96	3.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	5136427.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	16.23582219219074
#Duplication Level	Relative count
1	100.0
2	12.327788373446422
3	1.6646403650800052
4	0.4546214081163462
5	0.23163220033304333
6	0.13252588576268648
7	0.0800917309609279
8	0.0593485488415509
9	0.053010354305074586
10++	0.23624179635957154
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	5165805	7.200785	9.490885	145-146
AAAAA	4403060	6.453753	7.440298	75-79
ATTTT	2434060	3.427175	4.6623073	1
GGGGG	2824040	3.3784244	4.722837	145-146
AAAAT	2251725	3.2674572	4.125959	1
CCGGC	2260485	3.0046227	3.388328	4
>>END_MODULE