Table of Contents
	Project Information
		report description
		projects db info
		taxonomy summary
		genome size estimates
		contamination summary
		project base counts
	Libraries and Reads
		assembled average insert size estimates
		library / read quality summary
		reads2plates summary
		trimmed read length histograms
		library vector screening
		GC Content of reads histogram
	Contigs and Assemblies
		contig size and read count table
		depth summary
		depth histogram
		depth values
	Assembler Specific Info
		reads in assembly summary from assembler
		assembly parameters

Project Information

------------------------------------------------------------------- Assembly QC Report Date: 12-13-2005 Runby: Kerrie Barry Description: analysis of libraries in progress (or initial analysis of completed) ------------------------------------------------------------------- ------------------------------------------------------------------- Project information from 'PROJECTS' db ------------------------------------------------------------------- Project Size(KB) TaxID GenusSpecies 3634500 9000 NULL Jannaschia sp. 3634500 4500 NULL Jannaschia sp. ------------------------------------------------------------------- Taxonomy summary Command: /home/copeland/scripts/tax2tree.sh Jannaschia_sp. -------------------------------------------------------------------

Error occurred: Empty result - nothing todo



pmfetch need params:

  • (id=NNNNNN[,NNNN,etc]) or (query_key=NNN, where NNN - number in the history, 0 - clipboard content for current database)
  • db=db_name (mandatory)
  • report=[docsum, brief, abstract, citation, medline, asn.1, mlasn1, uilist, sgml, gen] (Optional; default is asn.1)
  • mode=[html, file, text, asn.1, xml] (Optional; default is html)
  • dispstart - first element to display, from 0 to count - 1, (Optional; default is 0)
  • dispmax - number of items to display (Optional; default is all elements, from dispstart)
  • See help. ------------------------------------------------------------------- Genome size estimates ------------------------------------------------------------------- # contigs: 3746358 # phrap: 2952475 # db: altered. 9000000 4500000 5049708 +/- 2345426 ------------------------------------------------------------------- Contam Summary with *.contigs: Command: /psf/QC/bin/sparc/summarizeCrossMatchHits -o ------------------------------------------------------------------- Number of reads with X's: 3159 Number of reads with percent X's >= 20%: 229 = 1.6% Number of reads with percent X's >= 50%: 198 = 1.4% Number of reads with percent X's >= 80%: 141 = 1.0% Total reads in project: 14471 Total bp X'd : 276844 reads >= 20% >= 50% >= 80% screened Nr with L09136 2968 92 76 50 Nr with LRS 20 20 20 20 Nr with pMCL200_JGI_XZX+XZK 171 117 102 71 ------------------------------------------------------------------- Contam Summary with *.singlets: Command: /psf/QC/bin/sparc/summarizeCrossMatchHits -o -s ------------------------------------------------------------------- Number of reads with X's: 196 Number of reads with percent X's >= 20%: 144 = 14.5% Number of reads with percent X's >= 50%: 142 = 14.3% Number of reads with percent X's >= 80%: 122 = 12.2% Total reads in project: 996 Total bp X'd : 126678 reads >= 20% >= 50% >= 80% screened Nr with L09136 94 47 46 43 Nr with LRS 20 20 20 20 Nr with pMCL200_JGI_XZX+XZK 82 77 76 59 ------------------------------------------------------------------- Base Count for Project: Command: /home/copeland/scripts/projectBaseCount.pl phrap.out ------------------------------------------------------------------- A = 2770381 C = 4204335 G = 4266147 T = 2667507 N = 105566 X = 276844 GC fraction = 0.59 Total = 14290780 ------------------------------------------------------------------- Base Count for contigs: Command: /psf/QC/bin/sparc/faCount 3634500_fasta.screen.contigs ------------------------------------------------------------------- A 744413 C 1227349 G 1220245 T 747729 N 1274 fraction GC = 0.62 total bases = 3941010

    Libraries and Reads

    ------------------------------------------------------------------- Histogram of Assembled Average Insert Sizes: Command: /home/copeland/scripts/phrapView2.pl -p phrap.out -C > reads.list ------------------------------------------------------------------- ------------------------------------------------------------------- Command: /usr/xpg4/bin/grep AICF reads.list > grep.reads.list.AICF Command: /home/copeland/scripts/histogram2.pl grep.reads.list.AICF 4 500 ------------------------------------------------------------------- #Found 1130 total values totalling 4152750.0000. <3675.000000 +/- 590.905113> #Range: [ 949 - 4800 ] #Most likely bin: [ 3500 - 4000 ] 560 counts #Median bin: [ 3500 - 4000 ] 560 counts #Histogram Bins Count Fraction Cum_Fraction | 500 - 1000 : [ 2 0.00 0.00 ] |X 1000 - 1500 : [ 17 0.02 0.02 ] |X 1500 - 2000 : [ 18 0.02 0.03 ] |XX 2000 - 2500 : [ 28 0.02 0.06 ] |XXX 2500 - 3000 : [ 39 0.03 0.09 ] |XXXXXXXXXXX 3000 - 3500 : [ 153 0.14 0.23 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 3500 - 4000 : [ 560 0.50 0.72 ] |XXXXXXXXXXXXXXXXXXXXXX 4000 - 4500 : [ 306 0.27 0.99 ] | 4500 - 5000 : [ 7 0.01 1.00 ] ------------------------------------------------------------------- Command: /usr/xpg4/bin/grep AICG reads.list > grep.reads.list.AICG Command: /home/copeland/scripts/histogram2.pl grep.reads.list.AICG 4 500 ------------------------------------------------------------------- #Found 457 total values totalling 2827877.0000. <6187.914661 +/- 1633.193278> #Range: [ 974 - 8513 ] #Most likely bin: [ 6000 - 6500 ] 110 counts #Median bin: [ 6500 - 7000 ] 85 counts #Histogram Bins Count Fraction Cum_Fraction | 500 - 1000 : [ 1 0.00 0.00 ] |XXX 1000 - 1500 : [ 9 0.02 0.02 ] |XXXX 1500 - 2000 : [ 10 0.02 0.04 ] |XXXX 2000 - 2500 : [ 12 0.03 0.07 ] |XX 2500 - 3000 : [ 5 0.01 0.08 ] |XXXX 3000 - 3500 : [ 11 0.02 0.11 ] |XXX 3500 - 4000 : [ 8 0.02 0.12 ] |XXXX 4000 - 4500 : [ 12 0.03 0.15 ] |X 4500 - 5000 : [ 2 0.00 0.15 ] |XX 5000 - 5500 : [ 5 0.01 0.16 ] |XXXXXXXXXXXXXX 5500 - 6000 : [ 38 0.08 0.25 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 6000 - 6500 : [ 110 0.24 0.49 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 6500 - 7000 : [ 85 0.19 0.67 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXX 7000 - 7500 : [ 73 0.16 0.83 ] |XXXXXXXXXXXXXXXXXXXXX 7500 - 8000 : [ 59 0.13 0.96 ] |XXXXXX 8000 - 8500 : [ 16 0.04 1.00 ] | 8500 - 9000 : [ 1 0.00 1.00 ] ------------------------------------------------------------------- Estimated Assembled Average Insert Sizes: Command: /home/copeland/scripts/estInsertSize.pl -f phrap.out ------------------------------------------------------------------- # AICF 3560 +- 735 (n=580) # AICG 5811 +- 1991 (n=240) ------------------------------------------------------------------- Library / Read Quality summary extracted from 3634500_fasta.screen.trimQ15.SaF and database (md_run table) * note J15 is Jazz trimmed length, Q20 is count of Quality 20+ bases ------------------------------------------------------------------- DB |----J15---| |----Q20---| Fasta |----J15---| |----Q20---| LIB Reads %pass AvgLen %pass AvgNum Reads %pass AvgLen %pass AvgNun AICF 30720 -1 -1 97 731 6831 98 776 99 783 AICG 36480 -1 -1 91 574 7640 87 536 91 565 FWD |----J15---| |----Q20---| REV |----J15---| |----Q20---| LIB Reads %pass AvgLen %pass AvgNum Reads %pass AvgLen %pass AvgNun AICF 3456 98 768 99 784 3375 98 784 99 782 AICG 3821 84 541 89 559 3819 90 531 93 571 ------------------------------------------------------------------- reads2plates summary extracted from file: 3634500_fasta.screen.r2p [ from "READ/CLONE COUNT SUMMARY" to end ] ------------------------------------------------------------------- plate(s) reads clones N/plate avg% LIBRARY @ 40 6831 3840 96.00 100.00 AICF @ 40 7640 3836 95.90 99.90 AICG @ ] 14471 7676 95.95 cumulative total@@ LIBRARY PLATE ID COUNT [ AICF 40 AICG 40 ] for 80 total 96 well plate ids. Only indicates plates present in input file. Make no assumption regarding plates (not) present in project that do not appear above. ------------------------------------------------------------------- trimt JAZZ trim 15 readlength histogram: Command: /home/copeland/scripts/histogram2.pl 3634500_fasta.screen.trimQ15.SaF 4 50 ------------------------------------------------------------------- #Found 14471 total values totalling 8770305.0000. <606.060742 +/- 242.098412> #Range: [ 0 - 943 ] #Most likely bin: [ 800 - 850 ] 2461 counts #Median bin: [ 650 - 700 ] 1476 counts #Histogram Bins Count Fraction Cum_Fraction |XXXXXXXXXXXXXXXX 0 - 50 : [ 970 0.07 0.07 ] |XXX 50 - 100 : [ 158 0.01 0.08 ] |XXX 100 - 150 : [ 174 0.01 0.09 ] |XXX 150 - 200 : [ 179 0.01 0.10 ] |XXXX 200 - 250 : [ 216 0.01 0.12 ] |XXXX 250 - 300 : [ 268 0.02 0.14 ] |XXXXX 300 - 350 : [ 336 0.02 0.16 ] |XXXXXX 350 - 400 : [ 372 0.03 0.18 ] |XXXXXX 400 - 450 : [ 390 0.03 0.21 ] |XXXXXXX 450 - 500 : [ 420 0.03 0.24 ] |XXXXXXXXX 500 - 550 : [ 546 0.04 0.28 ] |XXXXXXXXXXXXXX 550 - 600 : [ 850 0.06 0.34 ] |XXXXXXXXXXXXXXXXXXXXX 600 - 650 : [ 1266 0.09 0.42 ] |XXXXXXXXXXXXXXXXXXXXXXXX 650 - 700 : [ 1476 0.10 0.53 ] |XXXXXXXXXXXXXXXXXXXXXXXX 700 - 750 : [ 1505 0.10 0.63 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 750 - 800 : [ 2359 0.16 0.79 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 800 - 850 : [ 2461 0.17 0.96 ] |XXXXXXXX 850 - 900 : [ 511 0.04 1.00 ] | 900 - 950 : [ 14 0.00 1.00 ] trimt JAZZ trim 15 readlength histogram for AICF ------------------------------------------------------------------- Command: /usr/xpg4/bin/grep AICF 3634500_fasta.screen.trimQ15.SaF > reads.trim15.AICF.rl Command: /home/copeland/scripts/histogram2.pl reads.trim15.AICF.rl 2 50 ------------------------------------------------------------------- #Found 6831 total values totalling 5176602.0000. <757.810277 +/- 143.488993> #Range: [ 0 - 943 ] #Most likely bin: [ 800 - 850 ] 2453 counts #Median bin: [ 750 - 800 ] 2199 counts #Histogram Bins Count Fraction Cum_Fraction |XX 0 - 50 : [ 148 0.02 0.02 ] | 50 - 100 : [ 13 0.00 0.02 ] | 100 - 150 : [ 11 0.00 0.03 ] | 150 - 200 : [ 4 0.00 0.03 ] | 200 - 250 : [ 18 0.00 0.03 ] | 250 - 300 : [ 24 0.00 0.03 ] | 300 - 350 : [ 13 0.00 0.03 ] | 350 - 400 : [ 29 0.00 0.04 ] | 400 - 450 : [ 10 0.00 0.04 ] | 450 - 500 : [ 7 0.00 0.04 ] | 500 - 550 : [ 19 0.00 0.04 ] |X 550 - 600 : [ 50 0.01 0.05 ] |XXX 600 - 650 : [ 156 0.02 0.07 ] |XXXXX 650 - 700 : [ 307 0.04 0.12 ] |XXXXXXXXXXXXXX 700 - 750 : [ 845 0.12 0.24 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 750 - 800 : [ 2199 0.32 0.56 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 800 - 850 : [ 2453 0.36 0.92 ] |XXXXXXXX 850 - 900 : [ 511 0.07 1.00 ] | 900 - 950 : [ 14 0.00 1.00 ] trimt JAZZ trim 15 readlength histogram for AICG ------------------------------------------------------------------- Command: /usr/xpg4/bin/grep AICG 3634500_fasta.screen.trimQ15.SaF > reads.trim15.AICG.rl Command: /home/copeland/scripts/histogram2.pl reads.trim15.AICG.rl 2 50 ------------------------------------------------------------------- #Found 7640 total values totalling 3593703.0000. <470.379974 +/- 231.536643> #Range: [ 0 - 828 ] #Most likely bin: [ 650 - 700 ] 1169 counts #Median bin: [ 550 - 600 ] 800 counts #Histogram Bins Count Fraction Cum_Fraction |XXXXXXXXXXXXXXXXXXXXXXXXXXXX 0 - 50 : [ 822 0.11 0.11 ] |XXXXX 50 - 100 : [ 145 0.02 0.13 ] |XXXXXX 100 - 150 : [ 163 0.02 0.15 ] |XXXXXX 150 - 200 : [ 175 0.02 0.17 ] |XXXXXXX 200 - 250 : [ 198 0.03 0.20 ] |XXXXXXXX 250 - 300 : [ 244 0.03 0.23 ] |XXXXXXXXXXX 300 - 350 : [ 323 0.04 0.27 ] |XXXXXXXXXXXX 350 - 400 : [ 343 0.04 0.32 ] |XXXXXXXXXXXXX 400 - 450 : [ 380 0.05 0.37 ] |XXXXXXXXXXXXXX 450 - 500 : [ 413 0.05 0.42 ] |XXXXXXXXXXXXXXXXXX 500 - 550 : [ 527 0.07 0.49 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXX 550 - 600 : [ 800 0.10 0.59 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 600 - 650 : [ 1110 0.15 0.74 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 650 - 700 : [ 1169 0.15 0.89 ] |XXXXXXXXXXXXXXXXXXXXXXX 700 - 750 : [ 660 0.09 0.98 ] |XXXXX 750 - 800 : [ 160 0.02 1.00 ] | 800 - 850 : [ 8 0.00 1.00 ] trimt JAZZ trim 15 readlength histogram for AICH ------------------------------------------------------------------- Library vector screening Command: /home/copeland/scripts/checkScreen.sh 3634500 ------------------------------------------------------------------- AICF.000001.000100 pUC18.fa LRS.fasta AICF.000101.000200 pUC18.fa LRS.fasta AICG.000001.000100 pMCL200.fa LRS.fasta AICG.000101.000200 pMCL200.fa LRS.fasta AICH.000001.000100 pCC1Fos.fa LRS.fasta AICF.000001.000100 pUC18.fa LRS.fasta AICF.000101.000200 pUC18.fa LRS.fasta AICG.000001.000100 pMCL200.fa LRS.fasta AICG.000101.000200 pMCL200.fa LRS.fasta AICH.000001.000100 pCC1Fos.fa LRS.fasta ------------------------------------------------------------------- GC content histogram: Command: /bin/nawk '{print $5+$6}' GC.3634500_fasta.screen.trimQ20 | /home/copeland/scripts/histogram2.pl - 1 0.005 ------------------------------------------------------------------- # GC.3634500_fasta.screen.trimQ20 | nawk 'NR>1 {print $5+$6}' | /home/jchapman/perlscripts/histogram2.pl - 1 0.005 #Found 13510 total values totalling 8364.1677. <0.619109 +/- 0.039343> #Range: [ 0.3871 - 0.7742 ] #Most likely bin: [ 0.625 - 0.63 ] 861 counts #Median bin: [ 0.62 - 0.625 ] 828 counts | 0.385 - 0.39 : [ 1 0.00 0.00 ] | 0.39 - 0.395 : [ 1 0.00 0.00 ] | 0.395 - 0.4 : [ 1 0.00 0.00 ] | 0.4 - 0.405 : [ 1 0.00 0.00 ] | 0.405 - 0.41 : [ 1 0.00 0.00 ] | 0.41 - 0.415 : [ 1 0.00 0.00 ] | 0.415 - 0.42 : [ 3 0.00 0.00 ] | 0.42 - 0.425 : [ 1 0.00 0.00 ] | 0.425 - 0.43 : [ 1 0.00 0.00 ] | 0.43 - 0.435 : [ 3 0.00 0.00 ] | 0.435 - 0.44 : [ 1 0.00 0.00 ] | 0.44 - 0.445 : [ 2 0.00 0.00 ] | 0.445 - 0.45 : [ 4 0.00 0.00 ] | 0.45 - 0.455 : [ 7 0.00 0.00 ] | 0.455 - 0.46 : [ 8 0.00 0.00 ] |X 0.46 - 0.465 : [ 11 0.00 0.00 ] |X 0.465 - 0.47 : [ 11 0.00 0.00 ] |X 0.47 - 0.475 : [ 11 0.00 0.01 ] |X 0.475 - 0.48 : [ 13 0.00 0.01 ] |X 0.48 - 0.485 : [ 17 0.00 0.01 ] |X 0.485 - 0.49 : [ 16 0.00 0.01 ] |X 0.49 - 0.495 : [ 20 0.00 0.01 ] | 0.495 - 0.5 : [ 9 0.00 0.01 ] |X 0.5 - 0.505 : [ 32 0.00 0.01 ] |X 0.505 - 0.51 : [ 25 0.00 0.01 ] |X 0.51 - 0.515 : [ 20 0.00 0.02 ] |XX 0.515 - 0.52 : [ 49 0.00 0.02 ] |XX 0.52 - 0.525 : [ 37 0.00 0.02 ] |XX 0.525 - 0.53 : [ 44 0.00 0.03 ] |XXX 0.53 - 0.535 : [ 55 0.00 0.03 ] |XXX 0.535 - 0.54 : [ 66 0.00 0.03 ] |XXXX 0.54 - 0.545 : [ 84 0.01 0.04 ] |XXXX 0.545 - 0.55 : [ 95 0.01 0.05 ] |XXXXX 0.55 - 0.555 : [ 114 0.01 0.06 ] |XXXXXXXX 0.555 - 0.56 : [ 162 0.01 0.07 ] |XXXXXXX 0.56 - 0.565 : [ 157 0.01 0.08 ] |XXXXXXXXX 0.565 - 0.57 : [ 202 0.01 0.10 ] |XXXXXXXXXXX 0.57 - 0.575 : [ 234 0.02 0.11 ] |XXXXXXXXXXXXXX 0.575 - 0.58 : [ 296 0.02 0.13 ] |XXXXXXXXXXXXXXXX 0.58 - 0.585 : [ 353 0.03 0.16 ] |XXXXXXXXXXXXXXXXXX 0.585 - 0.59 : [ 379 0.03 0.19 ] |XXXXXXXXXXXXXXXXXXXXXX 0.59 - 0.595 : [ 473 0.04 0.22 ] |XXXXXXXXXXXXXXXXXXXXXXXX 0.595 - 0.6 : [ 520 0.04 0.26 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXX 0.6 - 0.605 : [ 633 0.05 0.31 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 0.605 - 0.61 : [ 654 0.05 0.36 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 0.61 - 0.615 : [ 728 0.05 0.41 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 0.615 - 0.62 : [ 777 0.06 0.47 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 0.62 - 0.625 : [ 828 0.06 0.53 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 0.625 - 0.63 : [ 861 0.06 0.59 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 0.63 - 0.635 : [ 775 0.06 0.65 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 0.635 - 0.64 : [ 759 0.06 0.71 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 0.64 - 0.645 : [ 689 0.05 0.76 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 0.645 - 0.65 : [ 642 0.05 0.81 ] |XXXXXXXXXXXXXXXXXXXXXXXXX 0.65 - 0.655 : [ 531 0.04 0.85 ] |XXXXXXXXXXXXXXXXXXXX 0.655 - 0.66 : [ 435 0.03 0.88 ] |XXXXXXXXXXXXXXXXX 0.66 - 0.665 : [ 370 0.03 0.90 ] |XXXXXXXXXXXXXXX 0.665 - 0.67 : [ 317 0.02 0.93 ] |XXXXXXXXXXX 0.67 - 0.675 : [ 234 0.02 0.95 ] |XXXXXXXXXX 0.675 - 0.68 : [ 212 0.02 0.96 ] |XXXXXX 0.68 - 0.685 : [ 128 0.01 0.97 ] |XXXX 0.685 - 0.69 : [ 91 0.01 0.98 ] |XXXX 0.69 - 0.695 : [ 88 0.01 0.98 ] |XXX 0.695 - 0.7 : [ 67 0.00 0.99 ] |XX 0.7 - 0.705 : [ 50 0.00 0.99 ] |X 0.705 - 0.71 : [ 25 0.00 0.99 ] |X 0.71 - 0.715 : [ 14 0.00 1.00 ] |X 0.715 - 0.72 : [ 16 0.00 1.00 ] | 0.72 - 0.725 : [ 8 0.00 1.00 ] | 0.725 - 0.73 : [ 6 0.00 1.00 ] | 0.73 - 0.735 : [ 7 0.00 1.00 ] | 0.735 - 0.74 : [ 6 0.00 1.00 ] | 0.74 - 0.745 : [ 4 0.00 1.00 ] | 0.745 - 0.75 : [ 4 0.00 1.00 ] | 0.75 - 0.755 : [ 1 0.00 1.00 ] | 0.755 - 0.76 : [ 4 0.00 1.00 ] | 0.76 - 0.765 : [ 1 0.00 1.00 ] #... | 0.77 - 0.775 : [ 4 0.00 1.00 ]

    Contigs and Assemblies

    ------------------------------------------------------------------- Command: /usr/local/bin/contig > contig.out [ final 30 lines ] ------------------------------------------------------------------- Contig 1179. 47 reads; 10954 bp (untrimmed), 10954 (trimmed). Contig 1180. 48 reads; 10138 bp (untrimmed), 10119 (trimmed). Contig 1181. 48 reads; 10148 bp (untrimmed), 9927 (trimmed). Contig 1182. 50 reads; 15533 bp (untrimmed), 15527 (trimmed). Contig 1183. 50 reads; 11543 bp (untrimmed), 11455 (trimmed). Contig 1184. 51 reads; 9607 bp (untrimmed), 9511 (trimmed). Contig 1185. 51 reads; 11509 bp (untrimmed), 11486 (trimmed). Contig 1186. 53 reads; 10888 bp (untrimmed), 10526 (trimmed). Contig 1187. 53 reads; 11962 bp (untrimmed), 11800 (trimmed). Contig 1188. 54 reads; 13414 bp (untrimmed), 13209 (trimmed). Contig 1189. 55 reads; 11809 bp (untrimmed), 11795 (trimmed). Contig 1190. 56 reads; 11902 bp (untrimmed), 11791 (trimmed). Contig 1191. 57 reads; 11852 bp (untrimmed), 11755 (trimmed). Contig 1192. 58 reads; 11485 bp (untrimmed), 11286 (trimmed). Contig 1193. 59 reads; 13922 bp (untrimmed), 13661 (trimmed). Contig 1194. 59 reads; 12217 bp (untrimmed), 11946 (trimmed). Contig 1195. 59 reads; 15765 bp (untrimmed), 15682 (trimmed). Contig 1196. 60 reads; 16006 bp (untrimmed), 15724 (trimmed). Contig 1197. 62 reads; 14664 bp (untrimmed), 14663 (trimmed). Contig 1198. 65 reads; 13012 bp (untrimmed), 12991 (trimmed). Contig 1199. 68 reads; 12812 bp (untrimmed), 12726 (trimmed). Contig 1200. 77 reads; 17726 bp (untrimmed), 17658 (trimmed). Contig 1201. 95 reads; 18200 bp (untrimmed), 18128 (trimmed). Contig 1202. 96 reads; 21554 bp (untrimmed), 21438 (trimmed). Contig 1203. 104 reads; 24030 bp (untrimmed), 24009 (trimmed). Contig 1204. 144 reads; 28507 bp (untrimmed), 28430 (trimmed). Contig 1205. 193 reads; 42168 bp (untrimmed), 41855 (trimmed). -------------------------------------------------------------- Totals 13475 reads; 3941010 bp (untrimmed), 3746358 (trimmed). ------------------------------------------------------------------- Depth Summary Command: /home/copeland/scripts/depth_summary.pl depth.out ------------------------------------------------------------------- depth.out contains 3938421 bases = 2.97 +- 1.61 = 0.62 +- 1.64 ------------------------------------------------------------------- Histogram of All Contig Depth Values: Command: /home/copeland/scripts/histogram2.pl depth.out 9 0.5 ------------------------------------------------------------------- #Found 1199 total values totalling 3158.1300. <2.633970 +/- 0.845194> #Range: [ 1.05 - 6.04 ] #Most likely bin: [ 2.5 - 3 ] 255 counts #Median bin: [ 2.5 - 3 ] 255 counts #Histogram Bins Count Fraction Cum_Fraction |XXXXXXXXXXXXXX 1 - 1.5 : [ 90 0.08 0.08 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 1.5 - 2 : [ 223 0.19 0.26 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 2 - 2.5 : [ 246 0.21 0.47 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 2.5 - 3 : [ 255 0.21 0.68 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXX 3 - 3.5 : [ 188 0.16 0.84 ] |XXXXXXXXXXXXXXXXXXX 3.5 - 4 : [ 118 0.10 0.93 ] |XXXXXXXXX 4 - 4.5 : [ 59 0.05 0.98 ] |XXX 4.5 - 5 : [ 16 0.01 1.00 ] #... | 5.5 - 6 : [ 3 0.00 1.00 ] | 6 - 6.5 : [ 1 0.00 1.00 ] ------------------------------------------------------------------- Histogram of Major Contig Depth Values: Command: /home/copeland/scripts/histogram2.pl depth.out 9 0.5 3 10 10000000 5 2000 10000000 ------------------------------------------------------------------- #Found 447 total values totalling 1468.0500. <3.284228 +/- 0.656947> #Range: [ 1.78 - 6.04 ] #Most likely bin: [ 2.5 - 3 ] 127 counts #Median bin: [ 3 - 3.5 ] 118 counts #Histogram Bins Count Fraction Cum_Fraction |X 1.5 - 2 : [ 3 0.01 0.01 ] |XXXXXXXXXXXXX 2 - 2.5 : [ 42 0.09 0.10 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 2.5 - 3 : [ 127 0.28 0.38 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 3 - 3.5 : [ 118 0.26 0.65 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXX 3.5 - 4 : [ 91 0.20 0.85 ] |XXXXXXXXXXXXXXXX 4 - 4.5 : [ 51 0.11 0.97 ] |XXX 4.5 - 5 : [ 11 0.02 0.99 ] #... |X 5.5 - 6 : [ 3 0.01 1.00 ] | 6 - 6.5 : [ 1 0.00 1.00 ] ------------------------------------------------------------------- Sorted Depth Values: Command: sort -n -k 9 depth.out > sorted.depth.out [first and last 20 lines included] ------------------------------------------------------------------- Contig 145 2 reads 1792 bases = 1.05 +- 0.21 = -0.02 +- 0.98 Contig 79 2 reads 1695 bases = 1.07 +- 0.25 = 1.07 +- 0.25 Contig 108 2 reads 1680 bases = 1.08 +- 0.27 = 1.08 +- 0.27 Contig 90 2 reads 1709 bases = 1.08 +- 0.27 = 1.08 +- 0.27 Contig 142 2 reads 1688 bases = 1.09 +- 0.28 = 1.09 +- 0.28 Contig 148 2 reads 1652 bases = 1.10 +- 0.31 = 1.10 +- 0.31 Contig 46 2 reads 1662 bases = 1.12 +- 0.33 = -0.23 +- 0.91 Contig 7 2 reads 1564 bases = 1.14 +- 0.35 = 1.14 +- 0.35 Contig 109 2 reads 1356 bases = 1.15 +- 0.36 = 0.28 +- 0.88 Contig 13 2 reads 1283 bases = 1.16 +- 0.37 = -0.20 +- 0.89 Contig 143 2 reads 1597 bases = 1.16 +- 0.37 = 1.16 +- 0.37 Contig 10 2 reads 1652 bases = 1.17 +- 0.37 = 1.17 +- 0.37 Contig 112 2 reads 808 bases = 1.17 +- 0.37 = 1.17 +- 0.37 Contig 250 3 reads 2304 bases = 1.19 +- 0.40 = 0.49 +- 0.94 Contig 33 2 reads 1244 bases = 1.19 +- 0.39 = -0.07 +- 0.90 Contig 39 2 reads 1215 bases = 1.20 +- 0.40 = 0.02 +- 0.89 Contig 40 2 reads 1176 bases = 1.20 +- 0.40 = 0.41 +- 0.80 Contig 4 2 reads 1562 bases = 1.21 +- 0.41 = 1.21 +- 0.41 Contig 80 2 reads 1537 bases = 1.21 +- 0.40 = 1.21 +- 0.40 Contig 9 2 reads 1566 bases = 1.21 +- 0.41 = -0.01 +- 0.89 Contig 1178 47 reads 9342 bases = 4.50 +- 1.77 = 0.29 +- 1.99 Contig 1120 29 reads 5460 bases = 4.52 +- 1.26 = 0.27 +- 1.61 Contig 800 10 reads 1990 bases = 4.54 +- 2.90 = 1.90 +- 1.65 Contig 1087 25 reads 4696 bases = 4.62 +- 1.92 = 1.26 +- 2.27 Contig 1201 95 reads 18200 bases = 4.64 +- 1.84 = 0.30 +- 1.69 Contig 962 16 reads 3040 bases = 4.64 +- 2.13 = 0.44 +- 2.10 Contig 1184 51 reads 9607 bases = 4.67 +- 2.36 = 0.22 +- 2.29 Contig 838 11 reads 2009 bases = 4.69 +- 2.06 = 0.37 +- 2.72 Contig 1123 31 reads 5861 bases = 4.70 +- 1.84 = 1.70 +- 2.06 Contig 899 13 reads 2528 bases = 4.71 +- 2.13 = 1.04 +- 2.01 Contig 1199 68 reads 12812 bases = 4.72 +- 1.91 = 0.23 +- 2.01 Contig 691 8 reads 1467 bases = 4.73 +- 2.23 = 2.30 +- 1.20 Contig 819 11 reads 1932 bases = 4.78 +- 2.27 = 0.65 +- 1.33 Contig 621 7 reads 1286 bases = 4.89 +- 1.87 = 0.82 +- 1.23 Contig 804 11 reads 2013 bases = 4.96 +- 2.56 = 0.22 +- 1.16 Contig 482 6 reads 924 bases = 4.97 +- 1.24 = 1.35 +- 0.55 Contig 946 15 reads 2455 bases = 5.50 +- 2.84 = 2.65 +- 2.25 Contig 1093 25 reads 4172 bases = 5.53 +- 2.43 = 1.04 +- 2.28 Contig 1145 35 reads 5481 bases = 5.68 +- 2.33 = 1.04 +- 1.98 Contig 1106 28 reads 3815 bases = 6.04 +- 3.00 = 2.33 +- 2.81

    Assembler Specific Info

    ------------------------------------------------------------------- Reads in assembly summary ------------------------------------------------------------------- Small Inserts = 92 HQ Discrepant reads = 388 Chimeric reads = 15 Suspect alignments = 45 ------------------------------------------------------------------- Assembly parameters ------------------------------------------------------------------- phrap version SPS - 3.57 SUN/Ultra-2/3 Equivalent to Phil Green's version 0.990329 Score matrix (set by value of penalty: -2) A C G T N X A 1 -2 -2 -2 0 -3 C -2 1 -2 -2 0 -3 G -2 -2 1 -2 0 -3 T -2 -2 -2 1 0 -3 N 0 0 0 0 0 0 X -3 -3 -3 -3 0 -3 gap_init: -4 gap_ext: -3 ins_gap_ext: -3 del_gap_ext: -3 Using complexity-adjusted scores. Assumed background frequencies: A: 0.250 C: 0.250 G: 0.250 T: 0.250 N: 0.000 X: 0.000 minmatch: 30 maxmatch: 55 max_group_size: 20 minscore: 55 bandwidth: 14 indexwordsize: 10 vector_bound: 20 word_raw: 0 trim_penalty: -2 trim_score: 20 trim_qual: 13 maxgap: 30 repeat_stringency: 0.950000 qual_show: 20 confirm_length: 8 confirm_trim: 1 confirm_penalty: -5 confirm_score: 30 node_seg: 8 node_space: 4 forcelevel: 0 bypasslevel: 1 max_subclone_size: 50000 File generated in /psf/project/microbe4/3634500/edit_dir.13May04.QC