Table of Contents
	Project Information
		report description
		projects db info
		taxonomy summary
		genome size estimates
		contamination summary
		project base counts
	Libraries and Reads
		assembled average insert size estimates
		library / read quality summary
		reads2plates summary
		trimmed read length histograms
		library vector screening
		GC Content of reads histogram
	Contigs and Assemblies
		contig size and read count table
		depth summary
		depth histogram
		depth values
	Assembler Specific Info
		reads in assembly summary from assembler
		assembly parameters

Project Information

------------------------------------------------------------------- Assembly QC Report Date: 12-13-2005 Runby: Kerrie Barry Description: analysis of libraries in progress (or initial analysis of completed) ------------------------------------------------------------------- ------------------------------------------------------------------- Project information from 'PROJECTS' db ------------------------------------------------------------------- Project Size(KB) TaxID GenusSpecies 3634512 6700 NULL Clostridium beijerincki ------------------------------------------------------------------- Taxonomy summary Command: /home/copeland/scripts/tax2tree.sh Clostridium_beijerincki -------------------------------------------------------------------

Error occurred: Empty result - nothing todo



pmfetch need params:

  • (id=NNNNNN[,NNNN,etc]) or (query_key=NNN, where NNN - number in the history, 0 - clipboard content for current database)
  • db=db_name (mandatory)
  • report=[docsum, brief, abstract, citation, medline, asn.1, mlasn1, uilist, sgml, gen] (Optional; default is asn.1)
  • mode=[html, file, text, asn.1, xml] (Optional; default is html)
  • dispstart - first element to display, from 0 to count - 1, (Optional; default is 0)
  • dispmax - number of items to display (Optional; default is all elements, from dispstart)
  • See help. ------------------------------------------------------------------- Genome size estimates ------------------------------------------------------------------- # contigs: 4533662 # phrap: 3052361 # db: altered. 6700000 4762007 +/- 1497870 ------------------------------------------------------------------- Contam Summary with *.contigs: Command: /psf/QC/bin/sparc/summarizeCrossMatchHits -o ------------------------------------------------------------------- Number of reads with X's: 2737 Number of reads with percent X's >= 20%: 456 = 3.0% Number of reads with percent X's >= 50%: 412 = 2.7% Number of reads with percent X's >= 80%: 344 = 2.3% Total reads in project: 15236 Total bp X'd : 481373 reads >= 20% >= 50% >= 80% screened Nr with L09136 2316 50 43 23 Nr with LRS 18 18 16 14 Nr with pMCL200_JGI_XZX+XZK 403 388 353 307 ------------------------------------------------------------------- Contam Summary with *.singlets: Command: /psf/QC/bin/sparc/summarizeCrossMatchHits -o -s ------------------------------------------------------------------- Number of reads with X's: 531 Number of reads with percent X's >= 20%: 356 = 13.5% Number of reads with percent X's >= 50%: 347 = 13.2% Number of reads with percent X's >= 80%: 323 = 12.3% Total reads in project: 2635 Total bp X'd : 341740 reads >= 20% >= 50% >= 80% screened Nr with L09136 202 30 29 20 Nr with LRS 18 18 16 14 Nr with pMCL200_JGI_XZX+XZK 311 308 302 289 ------------------------------------------------------------------- Base Count for Project: Command: /home/copeland/scripts/projectBaseCount.pl phrap.out ------------------------------------------------------------------- A = 4915204 C = 2561863 G = 2475086 T = 4783678 N = 103290 X = 481373 GC fraction = 0.33 Total = 15320494 ------------------------------------------------------------------- Base Count for contigs: Command: /psf/QC/bin/sparc/faCount 3634512_fasta.screen.contigs ------------------------------------------------------------------- A 1674315 C 799798 G 774624 T 1699249 N 3902 fraction GC = 0.32 total bases = 4951888

    Libraries and Reads

    ------------------------------------------------------------------- Histogram of Assembled Average Insert Sizes: Command: /home/copeland/scripts/phrapView2.pl -p phrap.out -C > reads.list ------------------------------------------------------------------- ------------------------------------------------------------------- Command: /usr/xpg4/bin/grep AHYP reads.list > grep.reads.list.AHYP Command: /home/copeland/scripts/histogram2.pl grep.reads.list.AHYP 4 500 ------------------------------------------------------------------- #Found 146 total values totalling 761225.0000. <5213.869863 +/- 1791.711634> #Range: [ 1044 - 7333 ] #Most likely bin: [ 5500 - 6000 ] 46 counts #Median bin: [ 5500 - 6000 ] 46 counts #Histogram Bins Count Fraction Cum_Fraction |XXXXXXXXX 1000 - 1500 : [ 10 0.07 0.07 ] |XXXXXXXXXX 1500 - 2000 : [ 11 0.08 0.14 ] |XXX 2000 - 2500 : [ 3 0.02 0.16 ] |XXX 2500 - 3000 : [ 3 0.02 0.18 ] |XXX 3000 - 3500 : [ 3 0.02 0.21 ] |X 3500 - 4000 : [ 1 0.01 0.21 ] |X 4000 - 4500 : [ 1 0.01 0.22 ] |XX 4500 - 5000 : [ 2 0.01 0.23 ] |XXXXX 5000 - 5500 : [ 6 0.04 0.27 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 5500 - 6000 : [ 46 0.32 0.59 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 6000 - 6500 : [ 38 0.26 0.85 ] |XXXXXXXXXXXX 6500 - 7000 : [ 14 0.10 0.95 ] |XXXXXXX 7000 - 7500 : [ 8 0.05 1.00 ] ------------------------------------------------------------------- Command: /usr/xpg4/bin/grep AIFZ reads.list > grep.reads.list.AIFZ Command: /home/copeland/scripts/histogram2.pl grep.reads.list.AIFZ 4 500 ------------------------------------------------------------------- #Found 652 total values totalling 2073053.0000. <3179.529141 +/- 642.427887> #Range: [ 881 - 4996 ] #Most likely bin: [ 3000 - 3500 ] 214 counts #Median bin: [ 3000 - 3500 ] 214 counts #Histogram Bins Count Fraction Cum_Fraction | 500 - 1000 : [ 2 0.00 0.00 ] |XX 1000 - 1500 : [ 13 0.02 0.02 ] |XXX 1500 - 2000 : [ 15 0.02 0.05 ] |XXXXX 2000 - 2500 : [ 29 0.04 0.09 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 2500 - 3000 : [ 188 0.29 0.38 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 3000 - 3500 : [ 214 0.33 0.71 ] |XXXXXXXXXXXXXXXXXXXXXXX 3500 - 4000 : [ 123 0.19 0.90 ] |XXXXXXXXXXX 4000 - 4500 : [ 60 0.09 0.99 ] |X 4500 - 5000 : [ 8 0.01 1.00 ] ------------------------------------------------------------------- Estimated Assembled Average Insert Sizes: Command: /home/copeland/scripts/estInsertSize.pl -f phrap.out ------------------------------------------------------------------- # AIFZ 3017 +- 752 (n=336) # AHYP 4087 +- 2562 (n=109) ------------------------------------------------------------------- Library / Read Quality summary extracted from 3634512_fasta.screen.trimQ15.SaF and database (md_run table) * note J15 is Jazz trimmed length, Q20 is count of Quality 20+ bases ------------------------------------------------------------------- DB |----J15---| |----Q20---| Fasta |----J15---| |----Q20---| LIB Reads %pass AvgLen %pass AvgNum Reads %pass AvgLen %pass AvgNun AIGB 7680 79 548 87 488 96 92 603 95 626 FWD |----J15---| |----Q20---| REV |----J15---| |----Q20---| LIB Reads %pass AvgLen %pass AvgNum Reads %pass AvgLen %pass AvgNun AIGB 0 0 0 0 0 96 92 603 95 626 ------------------------------------------------------------------- reads2plates summary extracted from file: 3634512_fasta.screen.r2p [ from "READ/CLONE COUNT SUMMARY" to end ] ------------------------------------------------------------------- plate(s) reads clones N/plate avg% LIBRARY @ 40 7556 3834 95.85 99.84 AIFZ @ 40 7680 3840 96.00 100.00 AHYP @ ] 15236 7674 95.92 cumulative total@@ LIBRARY PLATE ID COUNT [ AIFZ 40 AHYP 40 ] for 80 total 96 well plate ids. Only indicates plates present in input file. Make no assumption regarding plates (not) present in project that do not appear above. ------------------------------------------------------------------- trimt JAZZ trim 15 readlength histogram: Command: /home/copeland/scripts/histogram2.pl 3634512_fasta.screen.trimQ15.SaF 4 50 ------------------------------------------------------------------- #Found 96 total values totalling 53173.0000. <553.885417 +/- 245.235893> #Range: [ 0 - 881 ] #Most likely bin: [ 600 - 650 ] 13 counts #Median bin: [ 600 - 650 ] 13 counts #Histogram Bins Count Fraction Cum_Fraction |XXXXXXXXXXXXXXXXXXXXXX 0 - 50 : [ 7 0.07 0.07 ] |XXX 50 - 100 : [ 1 0.01 0.08 ] |XXXXXXXXX 100 - 150 : [ 3 0.03 0.11 ] |XXXXXXXXX 150 - 200 : [ 3 0.03 0.15 ] #... |XXXXXX 250 - 300 : [ 2 0.02 0.17 ] |XXXXXXXXX 300 - 350 : [ 3 0.03 0.20 ] |XXXXXXXXXXXXXXX 350 - 400 : [ 5 0.05 0.25 ] |XXXXXX 400 - 450 : [ 2 0.02 0.27 ] |XXXXXXXXX 450 - 500 : [ 3 0.03 0.30 ] |XXXXXXXXX 500 - 550 : [ 3 0.03 0.33 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 550 - 600 : [ 12 0.12 0.46 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 600 - 650 : [ 13 0.14 0.59 ] |XXXXXXXXXXXXXXXXXXXXXXXXX 650 - 700 : [ 8 0.08 0.68 ] |XXXXXXXXXXXXXXXXXX 700 - 750 : [ 6 0.06 0.74 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 750 - 800 : [ 12 0.12 0.86 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 800 - 850 : [ 10 0.10 0.97 ] |XXXXXXXXX 850 - 900 : [ 3 0.03 1.00 ] trimt JAZZ trim 15 readlength histogram for AHYP trimt JAZZ trim 15 readlength histogram for AHYS trimt JAZZ trim 15 readlength histogram for AIFZ trimt JAZZ trim 15 readlength histogram for AWGG trimt JAZZ trim 15 readlength histogram for BAOF trimt JAZZ trim 15 readlength histogram for BAOG trimt JAZZ trim 15 readlength histogram for BAOH trimt JAZZ trim 15 readlength histogram for BFTN trimt JAZZ trim 15 readlength histogram for SCNC trimt JAZZ trim 15 readlength histogram for SCOU trimt JAZZ trim 15 readlength histogram for SCOW ------------------------------------------------------------------- Library vector screening Command: /home/copeland/scripts/checkScreen.sh 3634512 ------------------------------------------------------------------- AHYP.000001.000100 pMCL200.fa pMCL200.fa LRS.fasta AIFZ.000001.000100 pUC18.fa pUC18.fa LRS.fasta AIFZ.000101.000200 pUC18.fa pUC18.fa LRS.fasta AIFZ.000201.000300 pUC18.fa pUC18.fa LRS.fasta AIFZ.000301.000400 pUC18.fa pUC18.fa LRS.fasta AIFZ.000401.000500 pUC18.fa pUC18.fa LRS.fasta AWGG.000001.000100 pMCL200.fa pMCL200.fa LRS.fasta BAOF.000001.000100 pCC1Fos.fa pCC1Fos.fa LRS.fasta BFTN.000001.000100 pCC1Fos.fa pCC1Fos.fa LRS.fasta AHYP.000001.000100 pMCL200.fa pMCL200.fa LRS.fasta AIFZ.000001.000100 pUC18.fa pUC18.fa LRS.fasta AIFZ.000101.000200 pUC18.fa pUC18.fa LRS.fasta AIFZ.000201.000300 pUC18.fa pUC18.fa LRS.fasta AIFZ.000301.000400 pUC18.fa pUC18.fa LRS.fasta AIFZ.000401.000500 pUC18.fa pUC18.fa LRS.fasta AWGG.000001.000100 pMCL200.fa pMCL200.fa LRS.fasta BAOF.000001.000100 pCC1Fos.fa pCC1Fos.fa LRS.fasta BFTN.000001.000100 pCC1Fos.fa pCC1Fos.fa LRS.fasta ------------------------------------------------------------------- GC content histogram: Command: /bin/nawk '{print $5+$6}' GC.3634512_fasta.screen.trimQ20 | /home/copeland/scripts/histogram2.pl - 1 0.005 ------------------------------------------------------------------- # GC.3634512_fasta.screen.trimQ20 | nawk 'NR>1 {print $5+$6}' | /home/jchapman/perlscripts/histogram2.pl - 1 0.005 #Found 13446 total values totalling 4213.5877. <0.313371 +/- 0.065117> #Range: [ 0.0455 - 0.6667 ] #Most likely bin: [ 0.29 - 0.295 ] 634 counts #Median bin: [ 0.3 - 0.305 ] 625 counts #Entropy = 5.4874 bits | 0.045 - 0.05 : [ 1 0.00 0.00 ] #... | 0.075 - 0.08 : [ 1 0.00 0.00 ] #... | 0.085 - 0.09 : [ 1 0.00 0.00 ] #... | 0.095 - 0.1 : [ 3 0.00 0.00 ] | 0.1 - 0.105 : [ 1 0.00 0.00 ] #... | 0.11 - 0.115 : [ 1 0.00 0.00 ] | 0.115 - 0.12 : [ 1 0.00 0.00 ] #... | 0.125 - 0.13 : [ 6 0.00 0.00 ] #... | 0.14 - 0.145 : [ 3 0.00 0.00 ] | 0.145 - 0.15 : [ 3 0.00 0.00 ] | 0.15 - 0.155 : [ 4 0.00 0.00 ] | 0.155 - 0.16 : [ 1 0.00 0.00 ] | 0.16 - 0.165 : [ 6 0.00 0.00 ] | 0.165 - 0.17 : [ 6 0.00 0.00 ] |X 0.17 - 0.175 : [ 9 0.00 0.00 ] |X 0.175 - 0.18 : [ 17 0.00 0.00 ] |X 0.18 - 0.185 : [ 12 0.00 0.01 ] |X 0.185 - 0.19 : [ 14 0.00 0.01 ] |X 0.19 - 0.195 : [ 13 0.00 0.01 ] |X 0.195 - 0.2 : [ 16 0.00 0.01 ] |XX 0.2 - 0.205 : [ 38 0.00 0.01 ] |XXX 0.205 - 0.21 : [ 42 0.00 0.01 ] |XXXX 0.21 - 0.215 : [ 64 0.00 0.02 ] |XXXX 0.215 - 0.22 : [ 59 0.00 0.02 ] |XXXXXX 0.22 - 0.225 : [ 100 0.01 0.03 ] |XXXXXXXX 0.225 - 0.23 : [ 120 0.01 0.04 ] |XXXXXXXX 0.23 - 0.235 : [ 134 0.01 0.05 ] |XXXXXXXXXXXXXX 0.235 - 0.24 : [ 228 0.02 0.07 ] |XXXXXXXXXXXXXXX 0.24 - 0.245 : [ 243 0.02 0.09 ] |XXXXXXXXXXXXXXX 0.245 - 0.25 : [ 245 0.02 0.10 ] |XXXXXXXXXXXXXXXXXXXXXXX 0.25 - 0.255 : [ 371 0.03 0.13 ] |XXXXXXXXXXXXXXXXXXXXXXX 0.255 - 0.26 : [ 360 0.03 0.16 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXX 0.26 - 0.265 : [ 439 0.03 0.19 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 0.265 - 0.27 : [ 480 0.04 0.23 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 0.27 - 0.275 : [ 511 0.04 0.26 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 0.275 - 0.28 : [ 570 0.04 0.31 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 0.28 - 0.285 : [ 590 0.04 0.35 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 0.285 - 0.29 : [ 586 0.04 0.39 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 0.29 - 0.295 : [ 634 0.05 0.44 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 0.295 - 0.3 : [ 572 0.04 0.48 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 0.3 - 0.305 : [ 625 0.05 0.53 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 0.305 - 0.31 : [ 576 0.04 0.57 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 0.31 - 0.315 : [ 583 0.04 0.62 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 0.315 - 0.32 : [ 521 0.04 0.66 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXX 0.32 - 0.325 : [ 462 0.03 0.69 ] |XXXXXXXXXXXXXXXXXXXXXXXXXX 0.325 - 0.33 : [ 412 0.03 0.72 ] |XXXXXXXXXXXXXXXXXXXXXXXXX 0.33 - 0.335 : [ 402 0.03 0.75 ] |XXXXXXXXXXXXXXXXXXXXXXX 0.335 - 0.34 : [ 372 0.03 0.78 ] |XXXXXXXXXXXXXXXXXXXX 0.34 - 0.345 : [ 315 0.02 0.80 ] |XXXXXXXXXXXXXXXX 0.345 - 0.35 : [ 247 0.02 0.82 ] |XXXXXXXXXXXXX 0.35 - 0.355 : [ 199 0.01 0.83 ] |XXXXXXXXXXXX 0.355 - 0.36 : [ 184 0.01 0.85 ] |XXXXXXX 0.36 - 0.365 : [ 113 0.01 0.86 ] |XXXXXXX 0.365 - 0.37 : [ 113 0.01 0.86 ] |XXXXXX 0.37 - 0.375 : [ 100 0.01 0.87 ] |XXXXX 0.375 - 0.38 : [ 85 0.01 0.88 ] |XXXX 0.38 - 0.385 : [ 69 0.01 0.88 ] |XXX 0.385 - 0.39 : [ 52 0.00 0.89 ] |XXX 0.39 - 0.395 : [ 54 0.00 0.89 ] |XXX 0.395 - 0.4 : [ 46 0.00 0.90 ] |XXXX 0.4 - 0.405 : [ 65 0.00 0.90 ] |XXX 0.405 - 0.41 : [ 47 0.00 0.90 ] |XXX 0.41 - 0.415 : [ 50 0.00 0.91 ] |XXX 0.415 - 0.42 : [ 50 0.00 0.91 ] |XXX 0.42 - 0.425 : [ 55 0.00 0.91 ] |XXXX 0.425 - 0.43 : [ 67 0.00 0.92 ] |XXXX 0.43 - 0.435 : [ 62 0.00 0.92 ] |XXXX 0.435 - 0.44 : [ 57 0.00 0.93 ] |XXX 0.44 - 0.445 : [ 50 0.00 0.93 ] |XXXXX 0.445 - 0.45 : [ 75 0.01 0.94 ] |XXXX 0.45 - 0.455 : [ 67 0.00 0.94 ] |XXXX 0.455 - 0.46 : [ 64 0.00 0.95 ] |XXXX 0.46 - 0.465 : [ 56 0.00 0.95 ] |XXXX 0.465 - 0.47 : [ 56 0.00 0.96 ] |XXXX 0.47 - 0.475 : [ 61 0.00 0.96 ] |XXX 0.475 - 0.48 : [ 54 0.00 0.96 ] |XXXX 0.48 - 0.485 : [ 59 0.00 0.97 ] |XXX 0.485 - 0.49 : [ 54 0.00 0.97 ] |XXX 0.49 - 0.495 : [ 48 0.00 0.98 ] |XX 0.495 - 0.5 : [ 36 0.00 0.98 ] |XXX 0.5 - 0.505 : [ 45 0.00 0.98 ] |XX 0.505 - 0.51 : [ 25 0.00 0.98 ] |XX 0.51 - 0.515 : [ 34 0.00 0.99 ] |XX 0.515 - 0.52 : [ 39 0.00 0.99 ] |XXX 0.52 - 0.525 : [ 41 0.00 0.99 ] |X 0.525 - 0.53 : [ 21 0.00 0.99 ] |X 0.53 - 0.535 : [ 17 0.00 1.00 ] |X 0.535 - 0.54 : [ 12 0.00 1.00 ] |X 0.54 - 0.545 : [ 13 0.00 1.00 ] |X 0.545 - 0.55 : [ 8 0.00 1.00 ] | 0.55 - 0.555 : [ 7 0.00 1.00 ] | 0.555 - 0.56 : [ 3 0.00 1.00 ] | 0.56 - 0.565 : [ 3 0.00 1.00 ] #... | 0.57 - 0.575 : [ 2 0.00 1.00 ] #... | 0.59 - 0.595 : [ 1 0.00 1.00 ] #... | 0.6 - 0.605 : [ 1 0.00 1.00 ] | 0.605 - 0.61 : [ 1 0.00 1.00 ] | 0.61 - 0.615 : [ 1 0.00 1.00 ] #... | 0.62 - 0.625 : [ 1 0.00 1.00 ] | 0.625 - 0.63 : [ 1 0.00 1.00 ] | 0.63 - 0.635 : [ 1 0.00 1.00 ] #... | 0.665 - 0.67 : [ 1 0.00 1.00 ]

    Contigs and Assemblies

    ------------------------------------------------------------------- Command: /usr/local/bin/contig > contig.out [ final 30 lines ] ------------------------------------------------------------------- Contig 2274. 25 reads; 6785 bp (untrimmed), 6741 (trimmed). Contig 2275. 25 reads; 7688 bp (untrimmed), 7438 (trimmed). Contig 2276. 26 reads; 6718 bp (untrimmed), 6708 (trimmed). Contig 2277. 26 reads; 6741 bp (untrimmed), 6720 (trimmed). Contig 2278. 26 reads; 5626 bp (untrimmed), 5372 (trimmed). Contig 2279. 26 reads; 7379 bp (untrimmed), 7324 (trimmed). Contig 2280. 26 reads; 7075 bp (untrimmed), 6911 (trimmed). Contig 2281. 27 reads; 7336 bp (untrimmed), 7298 (trimmed). Contig 2282. 27 reads; 10045 bp (untrimmed), 9840 (trimmed). Contig 2283. 28 reads; 7544 bp (untrimmed), 7461 (trimmed). Contig 2284. 30 reads; 8122 bp (untrimmed), 7879 (trimmed). Contig 2285. 30 reads; 9111 bp (untrimmed), 8989 (trimmed). Contig 2286. 31 reads; 9774 bp (untrimmed), 9559 (trimmed). Contig 2287. 32 reads; 8418 bp (untrimmed), 8124 (trimmed). Contig 2288. 33 reads; 9180 bp (untrimmed), 9113 (trimmed). Contig 2289. 34 reads; 11218 bp (untrimmed), 11084 (trimmed). Contig 2290. 36 reads; 9491 bp (untrimmed), 9124 (trimmed). Contig 2291. 37 reads; 8016 bp (untrimmed), 7858 (trimmed). Contig 2292. 39 reads; 8691 bp (untrimmed), 8556 (trimmed). Contig 2293. 43 reads; 12241 bp (untrimmed), 11946 (trimmed). Contig 2294. 45 reads; 12005 bp (untrimmed), 11999 (trimmed). Contig 2295. 48 reads; 11560 bp (untrimmed), 11491 (trimmed). Contig 2296. 51 reads; 15998 bp (untrimmed), 15833 (trimmed). Contig 2297. 52 reads; 8779 bp (untrimmed), 8417 (trimmed). Contig 2298. 53 reads; 12369 bp (untrimmed), 12337 (trimmed). Contig 2299. 57 reads; 11852 bp (untrimmed), 11773 (trimmed). Contig 2300. 95 reads; 7354 bp (untrimmed), 7130 (trimmed). -------------------------------------------------------------- Totals 12601 reads; 4951888 bp (untrimmed), 4533662 (trimmed). ------------------------------------------------------------------- Depth Summary Command: /home/copeland/scripts/depth_summary.pl depth.out ------------------------------------------------------------------- depth.out contains 4897907 bases = 2.28 +- 1.39 = 0.76 +- 1.37 ------------------------------------------------------------------- Histogram of All Contig Depth Values: Command: /home/copeland/scripts/histogram2.pl depth.out 9 0.5 ------------------------------------------------------------------- #Found 2243 total values totalling 4693.4200. <2.092474 +/- 0.700323> #Range: [ 1.04 - 11.57 ] #Most likely bin: [ 1.5 - 2 ] 759 counts #Median bin: [ 1.5 - 2 ] 759 counts #Histogram Bins Count Fraction Cum_Fraction |XXXXXXXXXXXXXXXXXXXXXXX 1 - 1.5 : [ 428 0.19 0.19 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 1.5 - 2 : [ 759 0.34 0.53 ] |XXXXXXXXXXXXXXXXXXXXXXXXXX 2 - 2.5 : [ 486 0.22 0.75 ] |XXXXXXXXXXXXXXXXX 2.5 - 3 : [ 327 0.15 0.89 ] |XXXXXXXXX 3 - 3.5 : [ 177 0.08 0.97 ] |XX 3.5 - 4 : [ 45 0.02 0.99 ] |X 4 - 4.5 : [ 13 0.01 1.00 ] | 4.5 - 5 : [ 5 0.00 1.00 ] | 5 - 5.5 : [ 1 0.00 1.00 ] #... | 7.5 - 8 : [ 1 0.00 1.00 ] #... | 11.5 - 12 : [ 1 0.00 1.00 ] ------------------------------------------------------------------- Histogram of Major Contig Depth Values: Command: /home/copeland/scripts/histogram2.pl depth.out 9 0.5 3 10 10000000 5 2000 10000000 ------------------------------------------------------------------- #Found 318 total values totalling 935.7500. <2.942610 +/- 0.797772> #Range: [ 1.77 - 11.57 ] #Most likely bin: [ 2.5 - 3 ] 110 counts #Median bin: [ 2.5 - 3 ] 110 counts #Histogram Bins Count Fraction Cum_Fraction |XX 1.5 - 2 : [ 5 0.02 0.02 ] |XXXXXXXXXXXXXXXXXXXXXXXXXX 2 - 2.5 : [ 71 0.22 0.24 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 2.5 - 3 : [ 110 0.35 0.58 ] |XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 3 - 3.5 : [ 91 0.29 0.87 ] |XXXXXXXXX 3.5 - 4 : [ 26 0.08 0.95 ] |XXX 4 - 4.5 : [ 9 0.03 0.98 ] |X 4.5 - 5 : [ 3 0.01 0.99 ] | 5 - 5.5 : [ 1 0.00 0.99 ] #... | 7.5 - 8 : [ 1 0.00 1.00 ] #... | 11.5 - 12 : [ 1 0.00 1.00 ] ------------------------------------------------------------------- Sorted Depth Values: Command: sort -n -k 9 depth.out > sorted.depth.out [first and last 20 lines included] ------------------------------------------------------------------- Contig 164 2 reads 1725 bases = 1.04 +- 0.19 = 1.04 +- 0.19 Contig 480 2 reads 1917 bases = 1.04 +- 0.21 = 1.04 +- 0.21 Contig 616 2 reads 1757 bases = 1.05 +- 0.21 = 0.04 +- 0.98 Contig 666 2 reads 1709 bases = 1.05 +- 0.22 = 1.05 +- 0.22 Contig 404 2 reads 1944 bases = 1.06 +- 0.23 = 1.06 +- 0.23 Contig 563 2 reads 1754 bases = 1.06 +- 0.24 = 0.03 +- 0.97 Contig 577 2 reads 1369 bases = 1.06 +- 0.24 = 0.12 +- 0.96 Contig 587 2 reads 1920 bases = 1.06 +- 0.24 = 1.06 +- 0.24 Contig 614 2 reads 1827 bases = 1.06 +- 0.24 = 1.06 +- 0.24 Contig 621 2 reads 1883 bases = 1.06 +- 0.23 = 1.06 +- 0.23 Contig 148 2 reads 1751 bases = 1.07 +- 0.26 = 1.07 +- 0.26 Contig 156 2 reads 1051 bases = 1.07 +- 0.26 = -0.93 +- 0.26 Contig 631 2 reads 1900 bases = 1.07 +- 0.25 = -0.01 +- 0.97 Contig 683 2 reads 1556 bases = 1.07 +- 0.26 = 1.07 +- 0.26 Contig 95 2 reads 1030 bases = 1.07 +- 0.25 = 1.07 +- 0.25 Contig 458 2 reads 1910 bases = 1.08 +- 0.27 = 1.08 +- 0.27 Contig 548 2 reads 1833 bases = 1.08 +- 0.28 = 1.08 +- 0.28 Contig 572 2 reads 1550 bases = 1.08 +- 0.26 = 1.08 +- 0.26 Contig 184 2 reads 1222 bases = 1.09 +- 0.28 = -0.22 +- 0.93 Contig 299 2 reads 1560 bases = 1.09 +- 0.29 = 1.09 +- 0.29 Contig 2178 14 reads 2755 bases = 4.03 +- 1.32 = 2.05 +- 2.14 Contig 2291 37 reads 8016 bases = 4.07 +- 1.41 = 0.29 +- 1.45 Contig 1946 9 reads 1905 bases = 4.09 +- 2.08 = 2.22 +- 1.54 Contig 2169 14 reads 3104 bases = 4.10 +- 1.42 = 2.27 +- 0.91 Contig 2292 39 reads 8691 bases = 4.10 +- 1.82 = 0.16 +- 2.09 Contig 1608 6 reads 1216 bases = 4.11 +- 1.78 = -0.23 +- 0.98 Contig 2079 11 reads 2374 bases = 4.22 +- 2.58 = 0.33 +- 1.04 Contig 1968 9 reads 1804 bases = 4.26 +- 2.00 = 4.26 +- 2.00 Contig 2089 11 reads 2380 bases = 4.29 +- 1.70 = 0.66 +- 1.69 Contig 2238 19 reads 3876 bases = 4.37 +- 1.99 = 0.08 +- 1.27 Contig 2255 21 reads 4180 bases = 4.38 +- 1.53 = 2.55 +- 1.97 Contig 2299 57 reads 11852 bases = 4.46 +- 2.95 = 1.19 +- 1.92 Contig 1997 10 reads 1997 bases = 4.56 +- 2.68 = 2.15 +- 1.13 Contig 1772 7 reads 1447 bases = 4.61 +- 1.77 = 0.60 +- 0.52 Contig 2273 25 reads 4807 bases = 4.75 +- 2.04 = 1.37 +- 2.23 Contig 2072 11 reads 2091 bases = 4.84 +- 2.55 = 2.93 +- 1.40 Contig 2297 52 reads 8779 bases = 4.99 +- 4.93 = -0.03 +- 1.40 Contig 2203 16 reads 2519 bases = 5.46 +- 4.55 = 1.23 +- 2.83 Contig 2219 17 reads 2021 bases = 7.80 +- 6.33 = 7.24 +- 5.73 Contig 2300 95 reads 7354 bases = 11.57 +- 9.96 = 2.95 +- 4.77

    Assembler Specific Info

    ------------------------------------------------------------------- Reads in assembly summary ------------------------------------------------------------------- Small Inserts = 201 HQ Discrepant reads = 379 Chimeric reads = 62 Suspect alignments = 29 ------------------------------------------------------------------- Assembly parameters ------------------------------------------------------------------- phrap version SPS - 3.57 SUN/Ultra-2/3 Equivalent to Phil Green's version 0.990329 Score matrix (set by value of penalty: -2) A C G T N X A 1 -2 -2 -2 0 -3 C -2 1 -2 -2 0 -3 G -2 -2 1 -2 0 -3 T -2 -2 -2 1 0 -3 N 0 0 0 0 0 0 X -3 -3 -3 -3 0 -3 gap_init: -4 gap_ext: -3 ins_gap_ext: -3 del_gap_ext: -3 Using complexity-adjusted scores. Assumed background frequencies: A: 0.250 C: 0.250 G: 0.250 T: 0.250 N: 0.000 X: 0.000 minmatch: 30 maxmatch: 55 max_group_size: 20 minscore: 55 bandwidth: 14 indexwordsize: 10 vector_bound: 20 word_raw: 0 trim_penalty: -2 trim_score: 20 trim_qual: 13 maxgap: 30 repeat_stringency: 0.950000 qual_show: 20 confirm_length: 8 confirm_trim: 1 confirm_penalty: -5 confirm_score: 30 node_seg: 8 node_space: 4 forcelevel: 0 bypasslevel: 1 max_subclone_size: 50000 File generated in /psf/project/microbe4/3634512/edit_dir.02Dec04.QC