Command line: /usr/common/jgi/assemblers/spades/2.5.0/bin/spades.py -o spades2.5 --phred-offset 33 -t 24 -m 240 --sc --careful --12 /house/groupdirs/QAQC/rqc_archive/jigsaw/in-progress/7220.2.64196.GTCCGC_single_cell_20130903111000/contam_filter/7220.2.64196.GTCCGC.contam.artifact.clean.subsample.fastq System information: SPAdes version: 2.5.0 Python version: 2.7.4 OS: Linux-2.6.32-5-amd64-x86_64-with-debian-6.0.7 Output dir: /global/projectb/scratch/aclum/gutless_worm/7-alpha/PPPC/spades_troubleshooting/spades2.5 Mode: read error correction and assembling Debug mode turned OFF Dataset parameters: Single-cell mode Reads: Library number: 1, library type: paired-end orientation: fr left reads: not specified right reads: not specified interlaced reads: ['/house/groupdirs/QAQC/rqc_archive/jigsaw/in-progress/7220.2.64196.GTCCGC_single_cell_20130903111000/contam_filter/7220.2.64196.GTCCGC.contam.artifact.clean.subsample.fastq'] single reads: not specified Read error correction parameters: Dir for temp files: /global/projectb/scratch/aclum/gutless_worm/7-alpha/PPPC/spades_troubleshooting/spades2.5/corrected/tmp Iterations: 1 PHRED offset: 33 Corrected reads will be compressed (with gzip) Assembly parameters: k: [21, 33, 55] Other parameters: Threads: 24 Memory limit (in Gb): 240