##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	H0951.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	40249030	
Filtered Sequences	0	
Sequence length	151	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.11429184753024	34.0	33.0	34.0	31.0	34.0
2	33.31502225022566	34.0	34.0	34.0	31.0	34.0
3	33.263992771999725	34.0	34.0	34.0	31.0	34.0
4	36.56837730002437	37.0	37.0	37.0	35.0	37.0
5	36.60068110958202	37.0	37.0	37.0	35.0	37.0
6	36.606439981286506	37.0	37.0	37.0	36.0	37.0
7	36.61242420003662	37.0	37.0	37.0	37.0	37.0
8	36.614889427148924	37.0	37.0	37.0	37.0	37.0
9	38.56986218052957	39.0	39.0	39.0	39.0	39.0
10-14	38.91167505900142	39.4	39.4	39.4	39.0	39.4
15-19	40.26273191180012	41.0	41.0	41.0	39.0	41.0
20-24	40.25515818393636	41.0	41.0	41.0	39.0	41.0
25-29	40.21253159144457	41.0	41.0	41.0	39.0	41.0
30-34	40.14650904630496	41.0	41.0	41.0	39.0	41.0
35-39	40.06801215333637	41.0	40.2	41.0	39.0	41.0
40-44	39.924189536990085	41.0	40.0	41.0	38.4	41.0
45-49	39.625642794372936	41.0	40.0	41.0	37.4	41.0
50-54	39.23116650016162	41.0	39.0	41.0	36.0	41.0
55-59	38.64640695191909	40.0	37.6	41.0	35.0	41.0
60-64	37.76987715728801	38.8	36.0	41.0	35.0	41.0
65-69	36.755815377414066	36.8	35.0	39.4	34.6	41.0
70-74	35.793661914336816	35.6	35.0	37.8	34.0	39.8
75-79	35.079651733221894	35.0	35.0	36.4	34.0	38.2
80-84	34.61203141044641	35.0	35.0	35.4	34.0	36.6
85-89	34.41451415847786	35.0	35.0	35.0	34.0	36.0
90-94	34.313069562173304	35.0	35.0	35.0	34.0	36.0
95-99	34.243076034378966	35.0	35.0	35.0	34.0	35.2
100-104	33.84427941244795	34.8	34.4	35.0	33.0	35.0
105-109	34.16967386791681	35.0	35.0	35.0	34.0	35.0
110-114	34.15899410743564	35.0	35.0	35.0	34.0	35.0
115-119	34.19089078171574	35.0	35.0	35.0	34.0	35.0
120-124	34.161489516641765	35.0	35.0	35.0	34.0	35.0
125-129	34.12239459683873	35.0	35.0	35.0	34.0	35.0
130-134	34.084895084428126	35.0	35.0	35.0	34.0	35.0
135-139	34.03393376685103	35.0	35.0	35.0	34.0	35.0
140-144	33.974729010860635	35.0	35.0	35.0	33.6	35.0
145-149	33.88910867168725	35.0	35.0	35.0	33.0	35.0
150-151	33.56515247696653	35.0	34.5	35.0	32.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	11088.0
1	2015.0
2	87789.0
3	25391.0
4	21475.0
5	19228.0
6	19798.0
7	21030.0
8	22516.0
9	22572.0
10	22652.0
11	21992.0
12	20047.0
13	19606.0
14	18093.0
15	17525.0
16	17757.0
17	17040.0
18	17318.0
19	17390.0
20	19937.0
21	22008.0
22	25444.0
23	33189.0
24	41419.0
25	51281.0
26	64849.0
27	82297.0
28	97221.0
29	120025.0
30	155487.0
31	208437.0
32	295057.0
33	442950.0
34	774604.0
35	2051927.0
36	1.4546083E7
37	2.0297868E7
38	476625.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.57047271673229	31.966003930235487	23.359838913824383	27.103684439207843
2	23.404818540335743	31.053355323423563	26.505300361531948	19.036525774708746
3	21.184449713370096	29.651969237966526	26.944749793030464	22.218831255632914
4	21.208840864415656	27.537197537124193	28.270511088622087	22.983450509838065
5	22.982085890668692	27.848815239398412	28.162942370953175	21.006156498979717
6	22.320379452466202	28.317483456226245	27.28718800067632	22.07494909063123
7	23.403300133279288	28.05004663494965	27.788896882536328	20.75775634923474
8	21.438769664227124	28.02969366308186	28.98487957856369	21.546657094127326
9	21.351233581557977	27.17412286251175	29.66996389376153	21.80467966216874
10-14	22.138135078024458	28.068579901687148	27.97256555391574	21.82071946637266
15-19	22.083836447947768	27.96476145001774	27.89668865272126	22.054713449313233
20-24	22.182873227184466	27.784707976699032	27.889047611650486	22.14337118446602
25-29	22.276770239513112	27.76324450669916	27.796750807876553	22.163234445911172
30-34	22.297686143979032	27.69913279964193	27.755310667871274	22.247870388507767
35-39	22.351872947071552	27.723811114037417	27.71567442194479	22.208641516946237
40-44	22.440113027470417	27.682768354873083	27.666725912281148	22.21039270537535
45-49	22.418951432759386	27.642366456088286	27.658019221102325	22.280662890050003
50-54	22.428735347358483	27.66908020984062	27.640703753418943	22.261480689381955
55-59	22.406976296211973	27.664974432558896	27.618486006818816	22.309563264410315
60-64	22.411629129459232	27.63404734260706	27.595022503988844	22.359301023944866
65-69	22.449474653495123	27.62160746426722	27.605093713126337	22.32382416911131
70-74	22.43786643331309	27.614541363436444	27.5915467297327	22.356045473517767
75-79	22.38848452957333	27.606787300850467	27.600049534909697	22.40467863466651
80-84	22.44794105154555	27.640389620002182	27.575899993806495	22.33576933464578
85-89	22.41988613718896	27.629339802563784	27.58852206268145	22.36225199756581
90-94	22.398871928976963	27.586486703551078	27.589924538090322	22.424716829381637
95-99	22.428188724089804	27.585435973033167	27.617451214996624	22.3689240878804
100-104	22.43361838703096	27.621800974794024	27.58764910964244	22.356931528532574
105-109	22.394833074309354	27.59040591414753	27.62115673738647	22.393604274156647
110-114	22.415429381225096	27.64321570224533	27.608951594767372	22.3324033217622
115-119	22.47493391702816	27.60442341403738	27.584190800524926	22.336451868409526
120-124	22.461061597288563	27.564038818417114	27.59268474333707	22.382214840957253
125-129	22.45772447184324	27.630975928811637	27.601663659097113	22.30963594024801
130-134	22.47804589935084	27.632639886809713	27.560803755930834	22.328510457908614
135-139	22.448856997075904	27.617013536190644	27.51696010075218	22.41716936598127
140-144	22.45247525330802	27.664626879824578	27.558788625364123	22.324109241503283
145-149	22.50958178892705	27.70249415840903	27.481429159701232	22.306494892962686
150-151	22.416884870910494	27.67193135109555	27.457429310666793	22.453754467327162
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	44.67415715594013
2	42.44134431504449
3	43.40328096900301
4	44.19229137425372
5	43.988242389648406
6	44.395328543097435
7	44.16105648251403
8	42.98542675835445
9	43.155913243726715
10-14	43.95885454439712
15-19	44.138549897261
20-24	44.326244411650485
25-29	44.44000468542429
30-34	44.5455565324868
35-39	44.56051446401779
40-44	44.65050573284577
45-49	44.69961432280938
50-54	44.69021603674044
55-59	44.71653956062229
60-64	44.770930153404095
65-69	44.773298822606435
70-74	44.79391190683086
75-79	44.793163164239836
80-84	44.78371038619132
85-89	44.78213813475477
90-94	44.8235887583586
95-99	44.79711281197021
100-104	44.79054991556353
105-109	44.788437348466
110-114	44.747832702987296
115-119	44.811385785437686
120-124	44.843276438245816
125-129	44.76736041209125
130-134	44.80655635725946
135-139	44.86602636305717
140-144	44.7765844948113
145-149	44.81607668188974
150-151	44.87063933823766
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2001.0
1	2017.5
2	1446.5
3	769.5
4	646.0
5	554.5
6	491.5
7	482.5
8	488.5
9	504.5
10	537.5
11	655.5
12	795.0
13	898.5
14	2205.0
15	3640.0
16	7247.0
17	15758.5
18	18592.0
19	30202.0
20	46867.5
21	53667.0
22	56898.0
23	58629.0
24	81739.0
25	118543.5
26	145642.0
27	173855.5
28	213775.0
29	247068.5
30	279114.5
31	318485.5
32	364229.5
33	440823.0
34	543148.0
35	668083.0
36	802606.5
37	961079.5
38	1107899.5
39	1246596.0
40	1426933.5
41	1646286.0
42	1863044.5
43	2061258.5
44	2238716.5
45	2352591.5
46	2447258.0
47	2479015.0
48	2394854.0
49	2289241.5
50	2163945.5
51	1901836.0
52	1569666.0
53	1337360.5
54	1127308.5
55	889726.0
56	684795.5
57	478025.5
58	306737.0
59	201332.5
60	144254.0
61	99023.0
62	56213.0
63	29270.0
64	12576.5
65	7035.5
66	4532.5
67	3389.5
68	2735.5
69	2248.0
70	1737.5
71	1361.0
72	1222.5
73	1104.5
74	960.5
75	835.0
76	701.0
77	552.5
78	417.5
79	308.5
80	230.5
81	149.0
82	100.0
83	87.5
84	70.5
85	52.0
86	39.5
87	30.0
88	29.5
89	26.0
90	20.0
91	13.0
92	6.5
93	9.5
94	8.0
95	4.5
96	4.5
97	4.5
98	3.5
99	3.5
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022862662777214757
2	0.12651981923539524
3	0.06089836202263757
4	0.06694571272897756
5	0.06179030898384384
6	0.04893534080200193
7	0.04589675825727974
8	0.042935196202243876
9	0.031039257343593127
10-14	0.03530519865944596
15-19	0.035186438033413474
20-24	0.03641081536623367
25-29	0.03382193310000266
30-34	0.03470443883989254
35-39	0.03479885105305643
40-44	0.029852147989653414
45-49	0.03628658876996539
50-54	0.04245120938318265
55-59	0.04029910782943092
60-64	0.05296326395940473
65-69	0.05754275320423871
70-74	0.06177142654121106
75-79	0.054774984639381366
80-84	0.057207341394314354
85-89	0.05079128615025009
90-94	0.035777756631650504
95-99	0.04209542441147029
100-104	0.033424407991944155
105-109	0.0364232380258605
110-114	0.0418454804997785
115-119	0.042128220232885115
120-124	0.03735493749787262
125-129	0.04495909590864674
130-134	0.045466437327806405
135-139	0.04205865333897488
140-144	0.04195132155979908
145-149	0.036096770530867454
150-151	0.0347374831144999
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	4.024903E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	95.13730015724225
#Duplication Level	Relative count
1	100.0
2	36.21539102074804
3	27.03490389285962
4	22.80202622996322
5	21.31704947609465
6	18.97855804593713
7	17.8960516272292
8	16.53597946013462
9	16.306987717715632
10++	1110.7417944625633
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE