##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	H0952.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	51870140	
Filtered Sequences	0	
Sequence length	151	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.95979322592922	34.0	33.0	34.0	31.0	34.0
2	33.15329555694278	34.0	34.0	34.0	31.0	34.0
3	33.23164315731556	34.0	34.0	34.0	31.0	34.0
4	36.50683531989696	37.0	37.0	37.0	35.0	37.0
5	36.501296988980556	37.0	37.0	37.0	35.0	37.0
6	36.493711757863004	37.0	37.0	37.0	35.0	37.0
7	36.49008913413382	37.0	37.0	37.0	35.0	37.0
8	36.49402800917831	37.0	37.0	37.0	35.0	37.0
9	38.41661094417713	39.0	39.0	39.0	38.0	39.0
10-14	38.73524705350709	39.4	39.2	39.4	38.2	39.4
15-19	40.02893181703385	41.0	40.0	41.0	38.4	41.0
20-24	40.012860601494424	41.0	40.0	41.0	39.0	41.0
25-29	39.962411784506465	41.0	40.0	41.0	38.2	41.0
30-34	39.88721528031349	41.0	40.0	41.0	38.0	41.0
35-39	39.795238177494795	41.0	40.0	41.0	38.0	41.0
40-44	39.63761903476644	41.0	40.0	41.0	37.8	41.0
45-49	39.349146587998405	41.0	39.6	41.0	36.6	41.0
50-54	38.93370625566077	40.8	38.8	41.0	35.2	41.0
55-59	38.32282195498219	39.8	37.2	41.0	35.0	41.0
60-64	37.41827841220402	38.4	35.6	40.8	34.6	41.0
65-69	36.38902899818662	36.6	35.0	39.2	34.0	41.0
70-74	35.445650568901485	35.4	35.0	37.4	33.4	39.6
75-79	34.76810233402107	35.0	35.0	36.2	33.0	37.8
80-84	34.32648110068722	35.0	35.0	35.2	33.0	36.6
85-89	34.1432832454279	35.0	35.0	35.0	33.0	36.0
90-94	34.04761953601822	35.0	35.0	35.0	33.0	35.8
95-99	33.9719759730743	35.0	35.0	35.0	33.0	35.0
100-104	33.54464106323985	34.8	34.4	35.0	32.0	35.0
105-109	33.88562606540101	35.0	35.0	35.0	33.0	35.0
110-114	33.87695151777111	35.0	35.0	35.0	33.0	35.0
115-119	33.91367106778583	35.0	35.0	35.0	33.0	35.0
120-124	33.88012498520343	35.0	35.0	35.0	33.0	35.0
125-129	33.83493750739828	35.0	35.0	35.0	33.0	35.0
130-134	33.78727189477414	35.0	35.0	35.0	33.0	35.0
135-139	33.7257730324229	35.0	35.0	35.0	33.0	35.0
140-144	33.64955679703197	35.0	35.0	35.0	33.0	35.0
145-149	33.54100765103005	35.0	35.0	35.0	33.0	35.0
150-151	33.18145532285048	35.0	34.5	35.0	31.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	13713.0
1	2960.0
2	135292.0
3	39570.0
4	31360.0
5	32572.0
6	33974.0
7	36819.0
8	39665.0
9	39915.0
10	39045.0
11	39392.0
12	36553.0
13	33359.0
14	31115.0
15	30052.0
16	29544.0
17	28299.0
18	28530.0
19	29563.0
20	33119.0
21	39427.0
22	47285.0
23	60733.0
24	76935.0
25	96548.0
26	120889.0
27	153083.0
28	186115.0
29	233620.0
30	298994.0
31	398175.0
32	555625.0
33	827365.0
34	1417102.0
35	3409576.0
36	1.9193996E7
37	2.345112E7
38	539141.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.511375783952012	31.677149184922854	22.91820820401646	27.893266827108675
2	23.55948119806147	30.659568410659322	26.212737394029052	19.56821299725016
3	21.55328706832328	29.086700492009964	26.747297394242047	22.61271504542471
4	21.505294098322082	27.15420095718923	27.956638297480772	23.383866647007917
5	23.300607265172086	27.523923024793866	27.791372246218334	21.384097463815717
6	22.73145341112459	27.902516675238598	26.9913918417547	22.374638071882107
7	23.774135746536864	27.619489833215873	27.477281901500056	21.129092518747207
8	21.74616157705999	27.745274421123423	28.55720204927274	21.95136195254385
9	21.643177064165194	26.75251755308042	29.335854428360964	22.268450954393426
10-14	22.54189571903022	27.591525189864747	27.63869011304743	22.227888978057603
15-19	22.442712564633325	27.538652326383183	27.544113183960455	22.474521925023037
20-24	22.60200050715853	27.40519323221732	27.48873904292205	22.5040672177021
25-29	22.621795274211497	27.415374136609987	27.40976293176366	22.553067657414854
30-34	22.687441496806958	27.32237029033205	27.35480008182371	22.635388131037278
35-39	22.746848885979137	27.31872139001294	27.29509147487517	22.639338249132752
40-44	22.819855126962565	27.269408254280297	27.302085263660373	22.608651355096764
45-49	22.78489893304119	27.207724716258124	27.30877501232426	22.698601338376427
50-54	22.849772045467713	27.26880308540475	27.257058226223307	22.624366642904235
55-59	22.776402329088686	27.323513869805318	27.242958270266175	22.65712553083982
60-64	22.846532971152385	27.24606753478241	27.18157828938223	22.725821204682976
65-69	22.79299798923258	27.237407901135025	27.24677798234151	22.722816127290887
70-74	22.77126835244354	27.300299255507294	27.149439044314494	22.778993347734676
75-79	22.788015942407057	27.28245437551371	27.23250738348055	22.697022298598686
80-84	22.811078074584675	27.287154027657778	27.171335155268174	22.73043274248938
85-89	22.7840833737323	27.282719440113574	27.178661904489648	22.754535281664477
90-94	22.734960346092237	27.252954977442606	27.21282726365341	22.79925741281175
95-99	22.833983590145948	27.226600630480142	27.26646615663506	22.67294962273885
100-104	22.81255103305758	27.214369736483707	27.173799395853454	22.79927983460526
105-109	22.77869372898846	27.22104552943642	27.1990417947685	22.80121894680661
110-114	22.780326022545864	27.250329315469113	27.232793133660994	22.736551528324032
115-119	22.78858568757961	27.26277281967312	27.186862751129098	22.761778741618173
120-124	22.764840420242678	27.27882499359132	27.175101989209043	22.78123259695696
125-129	22.807012014857023	27.28124783983153	27.204016802407537	22.707723342903908
130-134	22.856533751479056	27.27066885616196	27.12204937765552	22.750748014703458
135-139	22.781417720175657	27.315492102591016	27.159578128534406	22.743512048698918
140-144	22.857580399769056	27.26964249456747	27.174123008096174	22.698654097567296
145-149	22.90887957993347	27.281334176565252	27.125830393675887	22.68395584982539
150-151	22.8392725107159	27.28637822484623	27.110399503518224	22.76394976091965
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	45.40464261106069
2	43.127694195311626
3	44.16600211374799
4	44.889160745329995
5	44.6847047289878
6	45.1060914830067
7	44.90322826528407
8	43.697523529603835
9	43.911628018558616
10-14	44.76978469708782
15-19	44.91723448965636
20-24	45.10606772486063
25-29	45.17486293162635
30-34	45.32282962784424
35-39	45.38618713511189
40-44	45.42850648205933
45-49	45.48350027141762
50-54	45.474138688371944
55-59	45.43352785992851
60-64	45.57235417583536
65-69	45.515814116523465
70-74	45.55026170017821
75-79	45.485038241005746
80-84	45.541510817074055
85-89	45.53861865539678
90-94	45.534217758903985
95-99	45.506933212884796
100-104	45.61183086766284
105-109	45.57991267579508
110-114	45.51687755086989
115-119	45.550364429197785
120-124	45.54607301719963
125-129	45.514735357760934
130-134	45.60728176618252
135-139	45.52492976887458
140-144	45.556234497336355
145-149	45.59283542975886
150-151	45.603222271635545
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2378.0
1	2484.0
2	1817.5
3	976.5
4	824.5
5	668.5
6	610.0
7	587.5
8	564.0
9	594.0
10	606.0
11	835.0
12	1307.5
13	1951.0
14	3464.0
15	5665.5
16	6336.5
17	7374.0
18	9771.5
19	13696.5
20	22742.5
21	34369.0
22	50658.0
23	77780.0
24	95186.0
25	105884.5
26	123075.0
27	151097.0
28	199185.0
29	267050.0
30	335535.5
31	379751.0
32	426022.0
33	546608.0
34	706849.5
35	784543.5
36	876157.5
37	1004413.5
38	1150027.5
39	1349170.0
40	1539216.0
41	1794140.0
42	2131098.5
43	2439580.5
44	2751196.5
45	3135534.5
46	3520476.0
47	3666781.5
48	3522298.5
49	3247979.0
50	2959439.0
51	2578241.5
52	2130634.5
53	1747544.5
54	1442791.0
55	1189873.5
56	934251.5
57	730120.0
58	562777.5
59	393093.0
60	251959.0
61	160707.0
62	103644.0
63	63046.0
64	41807.5
65	38358.0
66	23503.0
67	11147.0
68	6018.5
69	1895.5
70	928.0
71	659.5
72	406.5
73	296.5
74	203.0
75	139.5
76	111.5
77	95.0
78	82.5
79	73.0
80	63.5
81	59.5
82	49.5
83	42.0
84	37.5
85	27.5
86	26.5
87	28.5
88	23.5
89	19.0
90	17.0
91	13.5
92	12.0
93	10.0
94	6.5
95	6.0
96	6.0
97	7.0
98	6.5
99	9.0
100	50.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02262573418926573
2	0.12444732171534528
3	0.05691713961057364
4	0.06231523570208216
5	0.05787337377535515
6	0.047339374831068516
7	0.04399641103725573
8	0.04107758336491862
9	0.029735797898366962
10-14	0.033974460065077906
15-19	0.033696458116365215
20-24	0.034911415315246885
25-29	0.032289868506235
30-34	0.03316166102501362
35-39	0.03320638810691469
40-44	0.02838126135769057
45-49	0.03458945744121763
50-54	0.040624914449816404
55-59	0.03853662241898711
60-64	0.05040819245909111
65-69	0.05502240788245415
70-74	0.059043989470628
75-79	0.05239661971222749
80-84	0.0546167795190065
85-89	0.048521557875108874
90-94	0.03411750961150288
95-99	0.040159135872777675
100-104	0.03180249754483022
105-109	0.0347660522990684
110-114	0.04003999218047224
115-119	0.04027673725191411
120-124	0.035812511784236556
125-129	0.043053672112702994
130-134	0.04375658133947585
135-139	0.04030604120212515
140-144	0.0401471829457179
145-149	0.03450270232546124
150-151	0.033313964450452614
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	5.187014E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	95.72490498858745
#Duplication Level	Relative count
1	100.0
2	38.50270870047647
3	30.70295672606227
4	27.041315841002547
5	24.208602571633705
6	21.96984531035833
7	19.887735787481233
8	18.021016904901767
9	16.82005091051498
10++	1008.2435872332093
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE