##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	H0953.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	50287074	
Filtered Sequences	0	
Sequence length	151	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.17662405651202	34.0	33.0	34.0	31.0	34.0
2	33.316623751065734	34.0	34.0	34.0	31.0	34.0
3	33.38843264175601	34.0	34.0	34.0	31.0	34.0
4	36.61981200178798	37.0	37.0	37.0	35.0	37.0
5	36.621718694549614	37.0	37.0	37.0	37.0	37.0
6	36.617453224659684	37.0	37.0	37.0	36.0	37.0
7	36.62021683743222	37.0	37.0	37.0	36.0	37.0
8	36.619366439972225	37.0	37.0	37.0	36.0	37.0
9	38.569673510930464	39.0	39.0	39.0	38.0	39.0
10-14	38.906730254379084	39.4	39.4	39.4	38.8	39.4
15-19	40.24998612963641	41.0	41.0	41.0	39.0	41.0
20-24	40.240887242713704	41.0	41.0	41.0	39.0	41.0
25-29	40.199648024858234	41.0	41.0	41.0	39.0	41.0
30-34	40.13461866164653	41.0	40.2	41.0	39.0	41.0
35-39	40.05905860420513	41.0	40.0	41.0	39.0	41.0
40-44	39.931820765710086	41.0	40.0	41.0	38.0	41.0
45-49	39.67975804279247	41.0	40.0	41.0	37.6	41.0
50-54	39.331211328780036	41.0	39.2	41.0	36.4	41.0
55-59	38.79219849617816	40.2	38.0	41.0	35.2	41.0
60-64	37.941487691250444	39.0	36.4	41.0	35.0	41.0
65-69	36.915049107848276	37.2	35.0	39.4	34.6	41.0
70-74	35.907417301710574	35.8	35.0	37.8	34.0	40.0
75-79	35.144630534677766	35.0	35.0	36.4	34.0	38.2
80-84	34.64217641296847	35.0	35.0	35.6	34.0	36.8
85-89	34.42662139777709	35.0	35.0	35.0	34.0	36.0
90-94	34.31882428076845	35.0	35.0	35.0	34.0	36.0
95-99	34.24865146458909	35.0	35.0	35.0	34.0	35.4
100-104	33.84397990227071	34.8	34.4	35.0	32.6	35.0
105-109	34.172718257578474	35.0	35.0	35.0	33.8	35.0
110-114	34.16454001678443	35.0	35.0	35.0	34.0	35.0
115-119	34.198749646877445	35.0	35.0	35.0	34.0	35.0
120-124	34.17000695645962	35.0	35.0	35.0	34.0	35.0
125-129	34.13149614948764	35.0	35.0	35.0	34.0	35.0
130-134	34.096554355101276	35.0	35.0	35.0	34.0	35.0
135-139	34.046779106694494	35.0	35.0	35.0	33.8	35.0
140-144	33.989376224196306	35.0	35.0	35.0	33.0	35.0
145-149	33.906264456746875	35.0	35.0	35.0	33.0	35.0
150-151	33.580341570479916	35.0	34.5	35.0	32.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	13564.0
1	2486.0
2	92825.0
3	26237.0
4	21646.0
5	21512.0
6	21945.0
7	24394.0
8	26251.0
9	26759.0
10	26855.0
11	26908.0
12	24717.0
13	22878.0
14	22112.0
15	21931.0
16	21163.0
17	20554.0
18	20315.0
19	21693.0
20	23983.0
21	27023.0
22	31737.0
23	39339.0
24	49713.0
25	61380.0
26	75972.0
27	100132.0
28	118720.0
29	151583.0
30	201818.0
31	278565.0
32	399261.0
33	614233.0
34	1076719.0
35	2649712.0
36	1.69958E7
37	2.6349257E7
38	535382.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.10411823413932	32.34815889879522	23.830079418686946	26.71764344837851
2	22.959510893442108	31.380823184580127	26.982501493270394	18.67716442870737
3	20.81685433857987	29.989904078799917	27.423958253743884	21.769283328876323
4	20.719532582360838	27.97747703343591	28.80987158813646	22.493118796066796
5	22.57969039963322	28.258591554833462	28.632882450783303	20.52883559475002
6	21.90944804148679	28.740975050208657	27.678824544899012	21.67075236340554
7	23.032686069507857	28.425897187249	28.219107365958795	20.322309377284352
8	21.15800958058954	28.48774208431785	29.27516883228187	21.079079502810735
9	20.939053752925084	27.575063365265663	30.022473710667846	21.463409171141407
10-14	21.724528568840295	28.4386679581426	28.375457479562556	21.461345993454554
15-19	21.6889318861008	28.337942144948595	28.309004247196974	21.66412172175363
20-24	21.80485711398617	28.141936314807335	28.29087667879977	21.762329892406726
25-29	21.90097544557071	28.1252256797047	28.184077854226626	21.78972102049796
30-34	21.9367137763513	28.09762903825068	28.108705247940375	21.85695193745764
35-39	22.023789167017746	28.058354267409246	28.07124946614148	21.84660709943153
40-44	22.023847275375775	28.070404674500153	28.047672065196906	21.858075984927165
45-49	22.03359445575453	28.034746709949122	28.012291570783574	21.919367263512783
50-54	22.046320693581738	28.0443797361369	28.004135366960604	21.905164203320762
55-59	22.042847367057178	28.04119188685791	27.999617084660695	21.91634366142422
60-64	22.01441596083965	27.999275205608935	28.002757835017857	21.98355099853356
65-69	22.025663687243156	28.03045317220736	27.9992787530573	21.944604387492184
70-74	22.048429910762128	28.03683410631679	27.98485673712246	21.929879245798624
75-79	21.968932752270085	27.999233836562297	28.019363653146982	22.012469758020632
80-84	22.032366047445585	28.002542836245325	27.995656485505833	21.96943463080326
85-89	22.035730757834024	28.01146733867727	27.98707594208288	21.96572596140583
90-94	21.981925702197618	28.0078513866986	27.98307450357353	22.02714840753025
95-99	21.996818995805047	27.999171963625447	28.003545860308716	22.000463180260795
100-104	22.030179645753826	28.034230934300414	28.000940716406316	21.934648703539448
105-109	22.007863414860363	27.984382326685104	27.99184614857361	22.01590810988092
110-114	21.983469457871998	28.058532874568364	27.993506656766208	21.964491010793427
115-119	22.003967213241683	28.05782824265539	27.981147471129447	21.95705707297348
120-124	22.01100622662608	27.99448467231138	27.996269880885567	21.99823922017697
125-129	22.004668026833265	28.039362481043188	28.010527262143714	21.945442229979832
130-134	22.053155369505916	28.061585958344708	27.95412231312587	21.931136359023505
135-139	22.006319201059867	28.02416679734387	27.988192038052397	21.981321963543866
140-144	22.06571053277108	28.0433882395916	27.95552632709346	21.935374900543856
145-149	22.084321358795222	28.095039856226478	27.91521275035128	21.905426034627013
150-151	22.073167926122206	28.022166886962353	27.92242635914211	21.982238827773333
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.82176168251783
2	41.63667532214948
3	42.58613766745619
4	43.21265137842764
5	43.10852599438324
6	43.58020040489233
7	43.3549954467922
8	42.23708908340028
9	42.40246292406649
10-14	43.18587456229485
15-19	43.353053607854434
20-24	43.5671870063929
25-29	43.69069646606867
30-34	43.793665713808934
35-39	43.870396266449276
40-44	43.88192326030294
45-49	43.95296171926731
50-54	43.95148489690251
55-59	43.9591910284814
60-64	43.99796695937321
65-69	43.970268074735344
70-74	43.978309156560755
75-79	43.981402510290714
80-84	44.00180067824884
85-89	44.001456719239854
90-94	44.00907410972787
95-99	43.997282176065845
100-104	43.96482834929327
105-109	44.023771524741285
110-114	43.94796046866543
115-119	43.961024286215164
120-124	44.00924544680305
125-129	43.9501102568131
130-134	43.98429172852942
135-139	43.98764116460374
140-144	44.00108543331494
145-149	43.989747393422235
150-151	44.05540675389554
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2434.0
1	2534.0
2	1868.5
3	1009.0
4	817.5
5	663.0
6	587.5
7	596.0
8	615.0
9	620.5
10	627.5
11	631.5
12	684.5
13	915.0
14	1180.0
15	1449.5
16	2183.5
17	3753.5
18	6346.0
19	9253.5
20	15984.5
21	28484.5
22	46917.5
23	67615.5
24	80659.5
25	104619.0
26	137175.0
27	162442.0
28	204428.5
29	270762.5
30	368091.0
31	489294.5
32	588315.5
33	679783.5
34	803081.5
35	952376.0
36	1141333.5
37	1353671.0
38	1599418.0
39	1884835.0
40	2174816.0
41	2470941.5
42	2781748.0
43	3039420.5
44	3170969.0
45	3217547.0
46	3172850.0
47	3042623.0
48	2863617.0
49	2617190.5
50	2321878.0
51	1991527.5
52	1618509.0
53	1279258.0
54	1015793.5
55	795093.0
56	587348.0
57	400297.0
58	265188.0
59	173377.0
60	105271.0
61	61346.0
62	40139.5
63	25068.5
64	15862.5
65	10374.5
66	5525.5
67	2594.5
68	1376.0
69	742.0
70	443.5
71	260.5
72	190.0
73	159.0
74	137.0
75	107.5
76	86.5
77	76.5
78	60.5
79	51.5
80	46.0
81	42.0
82	35.0
83	25.0
84	20.5
85	21.0
86	22.0
87	18.5
88	13.0
89	9.5
90	11.0
91	9.5
92	6.5
93	6.5
94	5.0
95	4.0
96	2.5
97	3.5
98	4.0
99	6.0
100	31.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022620126993270675
2	0.12610994228854916
3	0.05797513691092864
4	0.06375395792564904
5	0.058941588051036733
6	0.04838022590059624
7	0.04476498274685857
8	0.04188949231764807
9	0.030717635311213375
10-14	0.03492070347938717
15-19	0.03451105546526728
20-24	0.035983799733506065
25-29	0.03323716945630998
30-34	0.034143565402115064
35-39	0.03420600689552945
40-44	0.02942784064151356
45-49	0.03571056848525329
50-54	0.0420784076639655
55-59	0.03994664712446781
60-64	0.05209967078219743
65-69	0.05679272570124084
70-74	0.060823980333395415
75-79	0.05429148651599812
80-84	0.05614564092553884
85-89	0.04989234410417277
90-94	0.0350698471738483
95-99	0.0411302514837113
100-104	0.03271377451788108
105-109	0.035638581795393386
110-114	0.040924632043614226
115-119	0.041276213446023925
120-124	0.03666548584632305
125-129	0.0440530701786308
130-134	0.04463850889395553
135-139	0.04120263589008977
140-144	0.04115809164001071
145-149	0.03537529346010468
150-151	0.03407933418436714
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	5.0287074E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	95.24321819945109
#Duplication Level	Relative count
1	100.0
2	37.44944283945522
3	29.896822121337184
4	26.264960792406107
5	23.326454808089146
6	22.87247214197276
7	22.50103177878663
8	21.57655798596781
9	19.463475030953365
10++	1347.4948411060668
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE