##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	H0954.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	52782674	
Filtered Sequences	0	
Sequence length	151	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.997321109574706	34.0	33.0	34.0	31.0	34.0
2	33.13005004255753	34.0	34.0	34.0	31.0	34.0
3	33.248708487182746	34.0	34.0	34.0	31.0	34.0
4	36.528066274171714	37.0	37.0	37.0	35.0	37.0
5	36.510413796011925	37.0	37.0	37.0	35.0	37.0
6	36.5146797981474	37.0	37.0	37.0	35.0	37.0
7	36.5220123557969	37.0	37.0	37.0	35.0	37.0
8	36.5107259249503	37.0	37.0	37.0	35.0	37.0
9	38.44490731939803	39.0	39.0	39.0	38.0	39.0
10-14	38.7715381717872	39.4	39.2	39.4	38.2	39.4
15-19	40.06766197559449	41.0	40.0	41.0	39.0	41.0
20-24	40.0383149099267	41.0	40.0	41.0	39.0	41.0
25-29	39.9705120396136	41.0	40.0	41.0	38.6	41.0
30-34	39.869717885077215	41.0	40.0	41.0	38.0	41.0
35-39	39.73344929436504	41.0	40.0	41.0	38.0	41.0
40-44	39.4980727653169	41.0	40.0	41.0	37.2	41.0
45-49	39.07209199746113	41.0	39.0	41.0	35.6	41.0
50-54	38.47653177631736	40.2	37.4	41.0	35.0	41.0
55-59	37.71092287215309	39.2	36.0	41.0	35.0	41.0
60-64	36.761130006410816	37.2	35.0	40.2	34.0	41.0
65-69	35.80386260082238	35.6	35.0	38.4	33.6	40.8
70-74	34.98752805513415	35.0	35.0	36.6	33.0	39.0
75-79	34.43273742819472	35.0	35.0	35.8	33.0	37.0
80-84	34.08171285145576	35.0	35.0	35.0	33.0	36.2
85-89	33.95866326893556	35.0	35.0	35.0	33.0	36.0
90-94	33.884926034630226	35.0	35.0	35.0	33.0	35.4
95-99	33.80937010883533	35.0	35.0	35.0	33.0	35.0
100-104	33.35537052177387	34.8	34.4	35.0	31.6	35.0
105-109	33.700756880941654	35.0	35.0	35.0	33.0	35.0
110-114	33.6764473357299	35.0	35.0	35.0	33.0	35.0
115-119	33.71013543194117	35.0	35.0	35.0	33.0	35.0
120-124	33.662294191461385	35.0	35.0	35.0	33.0	35.0
125-129	33.59939094408139	35.0	35.0	35.0	33.0	35.0
130-134	33.53710994255425	35.0	35.0	35.0	33.0	35.0
135-139	33.45757288082829	35.0	35.0	35.0	32.6	35.0
140-144	33.365008142634075	35.0	35.0	35.0	32.0	35.0
145-149	33.236071749604804	35.0	35.0	35.0	32.0	35.0
150-151	32.848701536038135	35.0	34.5	35.0	30.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	14102.0
1	3186.0
2	130943.0
3	43554.0
4	40085.0
5	39886.0
6	39347.0
7	43170.0
8	48252.0
9	49758.0
10	49491.0
11	49156.0
12	45856.0
13	44028.0
14	41586.0
15	41911.0
16	43083.0
17	43297.0
18	45146.0
19	45918.0
20	49694.0
21	55194.0
22	66671.0
23	79468.0
24	95585.0
25	112386.0
26	138610.0
27	172690.0
28	205646.0
29	253875.0
30	326920.0
31	435644.0
32	601803.0
33	902149.0
34	1582370.0
35	4279325.0
36	2.2655848E7
37	1.9577802E7
38	289239.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.093876934027627	31.28661058549289	22.292096975126512	28.32741550535297
2	24.42066333601061	31.09086197442279	25.766720923514306	18.72175376605229
3	20.286699210975204	29.177343733605504	26.97991762637762	23.55603942904167
4	21.560126866283664	26.511174166026247	28.34622790212728	23.58247106556281
5	23.750250656008813	27.29470955352274	27.95661202779564	20.998427762672804
6	22.356853780571942	27.699493763917598	26.972655457383127	22.970996998127333
7	24.277715211473968	27.398582929922988	27.423397587159354	20.900304271443694
8	22.177293948240468	27.20171944846991	28.417125501455736	22.203861101833887
9	22.06153040165623	26.285695756665312	29.16668103814071	22.486092803537755
10-14	22.735307985178018	27.412440344753293	27.316056015706625	22.536195654362064
15-19	22.671347138010105	27.26443530638038	27.365613098435343	22.69860445717417
20-24	22.84411473056853	27.14322679520262	27.282111243447055	22.730547230781795
25-29	22.848221820843463	27.18355364683076	27.19813066915893	22.770093863166842
30-34	22.885479934406135	27.056914440742275	27.115490219707517	22.94211540514407
35-39	22.95417619551089	27.075053852295984	27.09113651903759	22.879633433155536
40-44	23.015772526792244	27.066011422751373	27.039662539630115	22.878553510826265
45-49	23.018600945514464	26.99457869647604	27.048634920111237	22.938185437898262
50-54	23.01521365090655	27.011341709928395	27.04682871509475	22.926615924070305
55-59	23.04697567212219	27.028381713547333	26.98072676566466	22.94391584866581
60-64	23.022577064942208	26.983617932843313	26.987402564315854	23.006402437898625
65-69	23.031719057072184	27.033814950306738	26.987080607526664	22.947385385094414
70-74	23.042065971655816	27.02644903282932	26.999649882930022	22.931835112584846
75-79	23.00587406142775	26.99970764923194	26.994068313086935	23.00034997625337
80-84	23.025466493653038	26.992192685694466	27.029703892955265	22.952636927697235
85-89	23.03418007260122	27.049877589166538	26.981002694351158	22.934939643881084
90-94	22.965997168692354	27.034146489013256	27.02872805391644	22.971128288377948
95-99	22.98366469568891	27.08518543094695	27.003747816360924	22.92740205700322
100-104	22.99257337557849	27.090523017659045	26.97936155260161	22.937542054160854
105-109	22.949109148258856	27.06296025288055	26.999894537962128	22.988036060898466
110-114	22.986293411234808	27.07040942090308	26.992730761778176	22.950566406083936
115-119	22.97702830479059	27.089956267783933	26.972277532327603	22.960737895097875
120-124	22.98483998470882	27.03050396082272	26.9734095488739	23.01124650559456
125-129	23.018066115525908	27.069883434141722	26.968707088586847	22.943343361745523
130-134	22.994635978260057	27.07265567276166	26.98842596339519	22.944282385583097
135-139	22.992304963053254	27.018193112591426	26.99381340777323	22.995688516582092
140-144	23.016926439753764	27.12099306821984	26.939157334226195	22.922923157800206
145-149	23.047236583873293	27.10530576555866	26.92602121435936	22.921436436208687
150-151	23.05050816476507	27.06482635309586	26.863902539830736	23.02076294230833
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	46.42129243938059
2	43.14241710206289
3	43.842738640016876
4	45.142597931846474
5	44.74867841868162
6	45.32785077869927
7	45.17801948291766
8	44.381155050074355
9	44.547623205193986
10-14	45.271503639540086
15-19	45.369951595184276
20-24	45.57466196135033
25-29	45.618315684010305
30-34	45.82759533955021
35-39	45.83380962866642
40-44	45.89432603761851
45-49	45.95678638341273
50-54	45.94182957497685
55-59	45.990891520788004
60-64	46.02897950284083
65-69	45.979104442166594
70-74	45.97390108424066
75-79	46.00622403768112
80-84	45.97810342135027
85-89	45.969119716482304
90-94	45.9371254570703
95-99	45.91106675269213
100-104	45.930115429739345
105-109	45.93714520915732
110-114	45.93685981731874
115-119	45.937766199888465
120-124	45.99608649030338
125-129	45.96140947727143
130-134	45.93891836384316
135-139	45.98799347963534
140-144	45.939849597553966
145-149	45.96867302008198
150-151	46.0712711070734
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2362.0
1	2512.5
2	1910.0
3	1061.0
4	846.0
5	676.5
6	615.0
7	592.0
8	560.0
9	553.5
10	558.0
11	583.0
12	640.0
13	688.5
14	815.5
15	2300.0
16	4261.0
17	5803.0
18	7589.5
19	8043.0
20	8828.0
21	12449.5
22	23561.5
23	52242.5
24	76128.0
25	93473.5
26	124037.0
27	153455.5
28	178066.5
29	188947.5
30	223012.0
31	294530.0
32	369577.0
33	478716.0
34	602500.5
35	710318.5
36	851905.0
37	1058719.5
38	1248402.0
39	1411497.0
40	1668494.5
41	1944092.5
42	2205651.5
43	2579282.0
44	2920170.0
45	3084018.0
46	3165251.5
47	3149254.0
48	3091246.5
49	3103904.0
50	3044784.5
51	2832249.0
52	2516790.0
53	2167966.5
54	1865747.0
55	1515869.5
56	1147637.0
57	852988.5
58	605797.0
59	414550.0
60	270994.0
61	173729.5
62	111552.0
63	65998.0
64	32957.0
65	18213.0
66	10856.5
67	5815.0
68	3703.0
69	2573.0
70	1777.0
71	1369.5
72	1081.5
73	863.0
74	727.0
75	550.0
76	435.5
77	361.5
78	287.0
79	205.5
80	153.5
81	113.5
82	75.5
83	64.0
84	46.5
85	40.0
86	31.0
87	18.5
88	20.5
89	18.5
90	16.5
91	13.0
92	10.5
93	10.0
94	7.0
95	6.5
96	6.5
97	3.5
98	5.0
99	10.0
100	31.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022554749689263565
2	0.12529300808064403
3	0.05796030720232173
4	0.06364209588926852
5	0.058913271426908
6	0.04788124224248282
7	0.04458470595862574
8	0.04174665345677637
9	0.03051758991975283
10-14	0.03464659634333797
15-19	0.034349529165574294
20-24	0.03563858852622738
25-29	0.03304038745744484
30-34	0.033839513322117785
35-39	0.03370954643184618
40-44	0.029085301741249413
45-49	0.035469593677652636
50-54	0.04159016271134729
55-59	0.039580033402627536
60-64	0.05154570228859569
65-69	0.0560888597648539
70-74	0.0601466306917304
75-79	0.05349785802818554
80-84	0.05564932159367296
85-89	0.04954390904863971
90-94	0.034787930600105633
95-99	0.040829685892760946
100-104	0.032371607395260044
105-109	0.03536046695929047
110-114	0.040665995815217695
115-119	0.0408656825533318
120-124	0.036359658474294045
125-129	0.043781033147354376
130-134	0.044294838112976236
135-139	0.040876671007611325
140-144	0.04080391986203655
145-149	0.03506264195709372
150-151	0.033908854257743745
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	5.2782674E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	95.90321234876929
#Duplication Level	Relative count
1	100.0
2	33.206411051093596
3	26.97246081643496
4	22.961126361462853
5	21.163552643230318
6	19.631630213406535
7	18.533604887983707
8	17.869476666961834
9	17.187638360046048
10++	1493.4826883910387
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE