##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	H0955.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	55758600	
Filtered Sequences	0	
Sequence length	151	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.95433644316751	34.0	33.0	34.0	31.0	34.0
2	33.11413134476117	34.0	34.0	34.0	31.0	34.0
3	33.13450018472487	34.0	34.0	34.0	31.0	34.0
4	36.46469651318362	37.0	37.0	37.0	35.0	37.0
5	36.44927327802348	37.0	37.0	37.0	35.0	37.0
6	36.42531326109336	37.0	37.0	37.0	35.0	37.0
7	36.444675834759124	37.0	37.0	37.0	35.0	37.0
8	36.42455621195654	37.0	37.0	37.0	35.0	37.0
9	38.352724261369545	39.0	39.0	39.0	37.0	39.0
10-14	38.66296142657815	39.4	39.2	39.4	37.2	39.4
15-19	39.96035028139157	41.0	40.0	41.0	38.2	41.0
20-24	39.94064539999211	41.0	40.0	41.0	38.6	41.0
25-29	39.88905847707797	41.0	40.0	41.0	38.0	41.0
30-34	39.79527040492408	41.0	40.0	41.0	38.0	41.0
35-39	39.6970922368926	41.0	40.0	41.0	38.0	41.0
40-44	39.535823503459554	41.0	40.0	41.0	37.4	41.0
45-49	39.25412378718261	41.0	39.4	41.0	36.4	41.0
50-54	38.84763461779887	40.8	38.8	41.0	35.0	41.0
55-59	38.300683955479506	40.0	37.2	41.0	35.0	41.0
60-64	37.509186181145154	39.0	35.6	41.0	34.6	41.0
65-69	36.58583822047182	37.0	35.0	39.6	34.0	41.0
70-74	35.667075744369484	35.8	35.0	38.2	33.4	40.2
75-79	34.94142315624855	35.0	35.0	36.6	33.0	39.0
80-84	34.41686082505658	35.0	35.0	35.8	33.0	37.0
85-89	34.149751360328274	35.0	35.0	35.0	33.0	36.0
90-94	33.99814322454294	35.0	35.0	35.0	33.0	36.0
95-99	33.899531939467636	35.0	35.0	35.0	33.0	36.0
100-104	33.460625704375644	34.8	34.4	35.0	31.6	35.2
105-109	33.79729067802994	35.0	35.0	35.0	33.0	35.0
110-114	33.783136140433946	35.0	35.0	35.0	33.0	35.0
115-119	33.810133783846794	35.0	35.0	35.0	33.0	35.0
120-124	33.772314720957844	35.0	35.0	35.0	33.0	35.0
125-129	33.72410018544224	35.0	35.0	35.0	33.0	35.0
130-134	33.67847178731173	35.0	35.0	35.0	33.0	35.0
135-139	33.614591076533486	35.0	35.0	35.0	33.0	35.0
140-144	33.54253960823981	35.0	35.0	35.0	33.0	35.0
145-149	33.437446894290744	35.0	35.0	35.0	33.0	35.0
150-151	33.09013206034585	35.0	34.5	35.0	31.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	14777.0
1	3161.0
2	156899.0
3	47666.0
4	41695.0
5	40407.0
6	44128.0
7	47536.0
8	52380.0
9	52223.0
10	51124.0
11	50935.0
12	46745.0
13	44979.0
14	41894.0
15	40007.0
16	39717.0
17	39079.0
18	40849.0
19	42676.0
20	46275.0
21	53062.0
22	62460.0
23	77541.0
24	95220.0
25	117816.0
26	143203.0
27	178562.0
28	212339.0
29	262326.0
30	338312.0
31	443056.0
32	610315.0
33	902558.0
34	1521230.0
35	3564536.0
36	1.7968856E7
37	2.7166787E7
38	1055269.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	16.539697937110486	32.890746487944526	24.68997747508569	25.87957809985931
2	22.353833846452208	32.782858687151915	27.47716463888193	17.386142827513947
3	18.73416137846786	31.056810359174026	28.590580127943205	21.61844813441491
4	19.768238168147924	28.38707016829465	30.269805749099078	21.574885914458353
5	21.84928440122198	29.135871271051716	29.767797661099472	19.24704666662683
6	20.64805379974217	29.535665070096055	28.73311233228109	21.083168797880678
7	22.218246203072166	29.281463005671675	29.25062912070157	19.249661670554588
8	20.297699589190312	29.166545858581628	30.149976898145002	20.385777654083057
9	20.363389401267145	28.05623018124459	31.020239990576588	20.560140426911673
10-14	20.848263913459977	29.30134741549375	29.172570814303157	20.677817856743115
15-19	20.759045142189876	29.171031603762206	29.24211219906611	20.827811054981805
20-24	20.986878523547762	29.03924570471967	29.104903069880823	20.868972701851742
25-29	20.96551848358751	29.075997964067778	29.011316517295782	20.94716703504893
30-34	21.05981667597702	28.943176502274802	28.988866424623243	21.00814039712493
35-39	21.09319637802139	28.95719738656048	28.92596991917712	21.023636316241014
40-44	21.114909855004317	28.95306443292704	28.901233067400135	21.03079264466851
45-49	21.098806641924632	28.884827171606936	28.937745297243843	21.078620889224585
50-54	21.120276627124397	28.937732594425654	28.886199621981973	21.05579115646798
55-59	21.11364306612736	28.927938050517948	28.91904379187834	21.039375091476355
60-64	21.09385569015515	28.900981061052704	28.895134343463013	21.110028905329138
65-69	21.183523695250393	28.905719505973376	28.88014808170193	21.0306087170743
70-74	21.108478981297786	28.913255033694345	28.88866846736781	21.08959751764006
75-79	21.090468184442138	28.927361979590277	28.858897996884085	21.1232718390835
80-84	21.144411031279926	28.864778772288396	28.910995339328842	21.079814857102836
85-89	21.110513774581293	28.90799156812519	28.921116288608527	21.060378368684987
90-94	21.12811310164626	28.858287575210433	28.91683165487548	21.096767668267827
95-99	21.11979864185722	28.893910932391854	28.896453981983626	21.0898364437673
100-104	21.10974716308886	28.92363556482213	28.908342637888023	21.058274634200988
105-109	21.10129457115263	28.87888202595515	28.896668441169865	21.123154961722356
110-114	21.109912851978745	28.904819576578383	28.88571530572282	21.099552265720053
115-119	21.12106399179318	28.92265652965228	28.910579610485264	21.045699868069274
120-124	21.05323896363575	28.906451944532215	28.935838432716228	21.104470659115808
125-129	21.133500021117964	28.908081017913883	28.91636995358246	21.042049007385693
130-134	21.10566518450362	28.93084437232049	28.898797965862748	21.064692477313137
135-139	21.12032605759144	28.89592624077463	28.883285245304354	21.100462456329574
140-144	21.116077029254594	28.978086214626686	28.843250291195027	21.062586464923687
145-149	21.13610125421359	29.0149278848267	28.86285316413138	20.986117696828323
150-151	21.14426545733493	28.917595430662608	28.819672907880467	21.118466204121994
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.41927603696979
2	39.739976673966154
3	40.35260951288277
4	41.34312408260627
5	41.09633106784881
6	41.731222597622846
7	41.46790787362675
8	40.68347724327337
9	40.923529828178815
10-14	41.52608177020309
15-19	41.586856197171684
20-24	41.855851225399505
25-29	41.91268551863644
30-34	42.06795707310195
35-39	42.116832694262406
40-44	42.14570249967283
45-49	42.17742753114922
50-54	42.17606778359237
55-59	42.15301815760372
60-64	42.20388459548428
65-69	42.214132412324695
70-74	42.198076498937844
75-79	42.21374002352564
80-84	42.22422588838276
85-89	42.17089214326628
90-94	42.22488076991409
95-99	42.20963508562452
100-104	42.168021797289846
105-109	42.224449532874985
110-114	42.2094651176988
115-119	42.166763859862456
120-124	42.15770962275156
125-129	42.17554902850365
130-134	42.17035766181676
135-139	42.220788513921015
140-144	42.17866349417829
145-149	42.122218951041916
150-151	42.262731661456925
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2736.0
1	2758.0
2	2005.5
3	1115.0
4	914.0
5	744.0
6	666.5
7	678.5
8	697.5
9	704.0
10	738.5
11	777.0
12	795.0
13	855.0
14	900.0
15	960.5
16	1157.5
17	3553.0
18	6596.5
19	12245.5
20	19618.0
21	25582.5
22	46760.5
23	78555.0
24	129010.0
25	190843.5
26	249705.5
27	388512.5
28	621196.5
29	810014.5
30	899526.0
31	958154.0
32	1038085.0
33	1157301.0
34	1338522.0
35	1504808.0
36	1714353.0
37	1936235.5
38	2149168.5
39	2514085.5
40	2783853.5
41	2869448.0
42	2954911.5
43	3069682.5
44	3189754.5
45	3196171.5
46	3044617.5
47	2894140.0
48	2703110.0
49	2382999.0
50	2027580.5
51	1680277.5
52	1350759.5
53	1068411.5
54	833347.5
55	633409.0
56	454072.5
57	295082.0
58	193893.0
59	126033.0
60	75950.0
61	47872.5
62	29161.5
63	17072.5
64	10088.5
65	5894.5
66	3434.0
67	2226.0
68	1445.0
69	971.0
70	675.0
71	472.0
72	323.5
73	188.0
74	121.0
75	94.5
76	93.5
77	86.5
78	69.0
79	54.0
80	45.5
81	41.5
82	34.5
83	33.5
84	34.5
85	31.0
86	28.0
87	22.5
88	17.0
89	16.0
90	17.0
91	17.0
92	15.5
93	13.5
94	10.5
95	6.0
96	3.5
97	5.5
98	9.0
99	13.0
100	40.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022109593856373726
2	0.12509281079510606
3	0.0568271082846413
4	0.06248901514743914
5	0.05798388051349926
6	0.04741331382064829
7	0.04387125932143203
8	0.040919248331199136
9	0.029731736449623913
10-14	0.034023092401889575
15-19	0.033670501052752405
20-24	0.03503889982890532
25-29	0.03235554694701804
30-34	0.033211737740904544
35-39	0.033302486073897124
40-44	0.028524747751916296
45-49	0.03458910374363775
50-54	0.04059284128367642
55-59	0.038596019268776476
60-64	0.050547538854992774
65-69	0.05518323630794174
70-74	0.05926834604886062
75-79	0.052805486507910886
80-84	0.05475711370084615
85-89	0.04865150846685534
90-94	0.0341852198584613
95-99	0.04011721958585761
100-104	0.03192117449146858
105-109	0.03484879462540308
110-114	0.040122599921805786
115-119	0.04040811641612235
120-124	0.03597328483857199
125-129	0.04315388119500849
130-134	0.043806336600990696
135-139	0.04032238973001474
140-144	0.040199718070396316
145-149	0.03459556014677557
150-151	0.0333840878357778
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	5.57586E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	95.90385393606131
#Duplication Level	Relative count
1	100.0
2	33.31396193520267
3	23.187563562400115
4	20.209211099811128
5	17.58680807787302
6	16.41725991573442
7	15.40752578817376
8	14.405055934912102
9	14.288827546128141
10++	1198.0386459392707
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE