##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	H0957.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	43612686	
Filtered Sequences	0	
Sequence length	151	
%GC	48	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.074841457827205	34.0	33.0	34.0	31.0	34.0
2	33.21980994245573	34.0	34.0	34.0	31.0	34.0
3	33.30590519006328	34.0	34.0	34.0	31.0	34.0
4	36.55158129907431	37.0	37.0	37.0	35.0	37.0
5	36.56832067165045	37.0	37.0	37.0	35.0	37.0
6	36.555252960113485	37.0	37.0	37.0	35.0	37.0
7	36.530477416593875	37.0	37.0	37.0	35.0	37.0
8	36.54892282947214	37.0	37.0	37.0	35.0	37.0
9	38.49693946848401	39.0	39.0	39.0	38.0	39.0
10-14	38.821429613392766	39.4	39.4	39.4	38.2	39.4
15-19	40.13020022660379	41.0	41.0	41.0	39.0	41.0
20-24	40.08617795290114	41.0	40.2	41.0	39.0	41.0
25-29	40.00348212444425	41.0	40.0	41.0	39.0	41.0
30-34	39.8713344002706	41.0	40.0	41.0	38.0	41.0
35-39	39.65471754250587	41.0	40.0	41.0	37.6	41.0
40-44	39.26165784423367	41.0	39.4	41.0	36.2	41.0
45-49	38.59618882450854	40.4	37.8	41.0	35.0	41.0
50-54	37.773219379333796	39.2	35.8	41.0	35.0	41.0
55-59	36.87171009370989	37.6	35.0	40.6	34.0	41.0
60-64	35.95083035243461	35.6	35.0	39.2	33.4	41.0
65-69	35.16951730971122	35.0	35.0	37.2	33.0	40.2
70-74	34.56425457950469	35.0	35.0	36.0	33.0	38.4
75-79	34.175274208059555	35.0	35.0	35.0	33.0	36.8
80-84	33.913111139268054	35.0	35.0	35.0	33.0	36.0
85-89	33.831721682998385	35.0	35.0	35.0	33.0	36.0
90-94	33.75981664600984	35.0	35.0	35.0	33.0	35.2
95-99	33.667889783261685	35.0	35.0	35.0	33.0	35.0
100-104	33.16758482153564	34.8	33.8	35.0	31.6	35.0
105-109	33.516391171137684	35.0	35.0	35.0	32.4	35.0
110-114	33.4752930191	35.0	35.0	35.0	32.0	35.0
115-119	33.51767573774292	35.0	35.0	35.0	33.0	35.0
120-124	33.452795321984986	35.0	35.0	35.0	32.8	35.0
125-129	33.36977765597835	35.0	35.0	35.0	32.0	35.0
130-134	33.28386824420765	35.0	35.0	35.0	32.0	35.0
135-139	33.177938978580684	35.0	35.0	35.0	31.6	35.0
140-144	33.055759303611794	35.0	35.0	35.0	31.0	35.0
145-149	32.88758095752231	35.0	35.0	35.0	31.0	35.0
150-151	32.43709029982698	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	11734.0
1	2377.0
2	103654.0
3	36817.0
4	36293.0
5	35059.0
6	34681.0
7	37590.0
8	41412.0
9	44502.0
10	45648.0
11	45965.0
12	43008.0
13	42781.0
14	43906.0
15	44350.0
16	45700.0
17	46788.0
18	48156.0
19	49613.0
20	53087.0
21	58209.0
22	67655.0
23	79700.0
24	93027.0
25	108780.0
26	132873.0
27	163156.0
28	195365.0
29	243794.0
30	313715.0
31	412308.0
32	566543.0
33	856322.0
34	1534088.0
35	4656904.0
36	2.1233722E7
37	1.1542262E7
38	461142.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.133465529209083	29.700265399328035	21.31207151145241	29.85419756001047
2	26.392798569458858	29.505905119139715	24.92749759687463	19.173798714526797
3	21.85543889028366	27.676283470112146	25.79481496931112	24.673462670293077
4	22.692840501040067	25.616123346776433	26.82617647138452	24.864859680798983
5	24.73595969202702	26.340814426639287	26.78623127532776	22.13699460600593
6	23.465789884613468	26.244670761577815	26.013955505285175	24.27558384852354
7	25.33022649768702	26.298785064332193	26.297358240778863	22.073630197201926
8	22.880926649847012	26.205611135664338	27.351481545904978	23.561980668583676
9	23.248961628714802	25.198895492680766	27.849596085095413	23.702546793509015
10-14	23.916084544846296	26.215799506808736	26.086839004543588	23.78127694380138
15-19	23.87799147427478	26.07720556934802	26.111886682996882	23.932916273380318
20-24	24.04747738815646	25.933221131873797	26.037847635769978	23.98145384419977
25-29	24.059449047939417	25.93025100350298	25.997703211934343	24.012596736623255
30-34	24.1197695051285	25.837236925899106	25.91736873807165	24.125624830900747
35-39	24.19432043549819	25.854877134521548	25.850025064289245	24.10077736569102
40-44	24.244937070174434	25.861497003073946	25.78574661628647	24.10781931046515
45-49	24.23493935608729	25.776931800228752	25.812344568533916	24.17578427515004
50-54	24.239892223032182	25.798321666312756	25.789179253869587	24.172606856785475
55-59	24.26457343819129	25.815821311464184	25.757233624750047	24.162371625594478
60-64	24.237699604309146	25.77933220903147	25.78179285435206	24.20117533230733
65-69	24.253761460663807	25.809884033600483	25.75568858260735	24.180665923128362
70-74	24.290427898634864	25.814505056808752	25.74636866908001	24.14869837547638
75-79	24.24843723863234	25.795934511386974	25.742502493839037	24.213125756141647
80-84	24.26540340029621	25.803814819541692	25.786513877930627	24.144267902231466
85-89	24.267259127880983	25.81894846044317	25.750389628183036	24.16340278349281
90-94	24.270736096965003	25.778324189287087	25.760459438292283	24.190480275455624
95-99	24.213572130857035	25.810017499574595	25.776732491810883	24.199677877757484
100-104	24.251336366685877	25.843417768335584	25.748779462655076	24.156466402323467
105-109	24.21737076579752	25.78606889387857	25.774898945431907	24.221661394892006
110-114	24.246507502577224	25.78363816130737	25.779976731639692	24.189877604475715
115-119	24.280970600768054	25.837809749269603	25.73269337621833	24.148526273744018
120-124	24.203209089524545	25.817686673453117	25.771180589574623	24.207923647447714
125-129	24.256429989695015	25.804066877390873	25.76075874735406	24.178744385560044
130-134	24.275069948645395	25.84402514964978	25.70506997218103	24.1758349295238
135-139	24.279427805769192	25.789636438578377	25.713649598185956	24.21728615746648
140-144	24.300017849298655	25.821427527239127	25.719299278567103	24.15925534489511
145-149	24.313293608739556	25.857266037932096	25.692840920784143	24.136599432544205
150-151	24.31312225134245	25.77412317483691	25.650532607726444	24.26222196609419
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	48.98766308921956
2	45.56659728398566
3	46.52890156057674
4	47.557700181839046
5	46.87295429803295
6	47.741373733137
7	47.403856694888944
8	46.442907318430684
9	46.95150842222382
10-14	47.69736148864768
15-19	47.810907747655094
20-24	48.02893123235623
25-29	48.072045784562675
30-34	48.24539433602924
35-39	48.29509780118921
40-44	48.35275638063958
45-49	48.41072363123733
50-54	48.41249907981766
55-59	48.42694506378577
60-64	48.43887493661647
65-69	48.43442738379217
70-74	48.43912627411124
75-79	48.461562994773985
80-84	48.40967130252768
85-89	48.4306619113738
90-94	48.46121637242063
95-99	48.41325000861452
100-104	48.40780276900934
105-109	48.43903216068952
110-114	48.436385107052935
115-119	48.42949687451207
120-124	48.41113273697226
125-129	48.43517437525506
130-134	48.45090487816919
135-139	48.49671396323566
140-144	48.45927319419377
145-149	48.449893041283765
150-151	48.575344217436644
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1930.0
1	2094.5
2	1574.5
3	807.0
4	691.0
5	600.0
6	523.0
7	498.0
8	490.0
9	498.5
10	497.5
11	501.5
12	949.5
13	2871.0
14	4354.0
15	3889.0
16	3686.5
17	4434.0
18	6207.0
19	10549.5
20	19334.0
21	31353.5
22	42774.5
23	64539.0
24	89767.0
25	109535.0
26	140528.0
27	178861.0
28	219638.0
29	244596.5
30	258554.5
31	308515.0
32	360495.5
33	414864.5
34	492750.0
35	574577.5
36	628030.0
37	647193.0
38	696518.0
39	777521.0
40	875770.0
41	983925.0
42	1065120.0
43	1136728.5
44	1276918.5
45	1422542.5
46	1562174.5
47	1750240.5
48	1951419.5
49	2146540.0
50	2333501.0
51	2488417.0
52	2562069.5
53	2535941.5
54	2435006.0
55	2222814.0
56	1903882.0
57	1557676.0
58	1253570.5
59	999418.0
60	770881.0
61	592319.5
62	436364.5
63	304885.5
64	212690.0
65	147488.5
66	103136.0
67	76707.5
68	55495.5
69	41005.0
70	28339.0
71	14704.5
72	8082.0
73	4404.5
74	1827.0
75	864.5
76	604.0
77	448.0
78	329.5
79	247.0
80	211.5
81	182.0
82	159.0
83	138.0
84	117.5
85	104.0
86	88.0
87	86.0
88	80.5
89	60.0
90	49.0
91	50.0
92	38.0
93	28.0
94	26.5
95	25.0
96	22.5
97	16.5
98	13.0
99	13.0
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02226874996875909
2	0.12332420892398144
3	0.05824681378257693
4	0.06417398827487947
5	0.05932906769374397
6	0.04782324115510794
7	0.0443655316253624
8	0.04139391919131053
9	0.030165534862952488
10-14	0.03438449078784095
15-19	0.034149238136811845
20-24	0.03556533986464397
25-29	0.03304084504219713
30-34	0.03386675152271062
35-39	0.03393737317623592
40-44	0.028992940265132945
45-49	0.03534888908241056
50-54	0.041422809867752694
55-59	0.03938624646966252
60-64	0.05157857051042442
65-69	0.05623547240360293
70-74	0.0602664096405344
75-79	0.05380406976080308
80-84	0.055733783514273806
85-89	0.04932463916576934
90-94	0.03462524642485904
95-99	0.040546459349006844
100-104	0.03224061916296556
105-109	0.03519526405688473
110-114	0.040568929875128536
115-119	0.04073310229046659
120-124	0.0362495444559411
125-129	0.04361941844168919
130-134	0.044033518137360304
135-139	0.04074273251594731
140-144	0.040574891443283266
145-149	0.03487654945168935
150-151	0.033702349816289695
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	4.3612686E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	94.90999379267535
#Duplication Level	Relative count
1	100.0
2	42.957317073170735
3	34.3140243902439
4	31.21951219512195
5	29.817073170731707
6	26.966463414634145
7	26.615853658536587
8	26.653963414634145
9	24.375
10++	1181.471036585366
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE