##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	H0958.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	58455140	
Filtered Sequences	0	
Sequence length	151	
%GC	47	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.98485228159577	34.0	33.0	34.0	31.0	34.0
2	33.137123442010406	34.0	34.0	34.0	31.0	34.0
3	33.2126906376411	34.0	34.0	34.0	31.0	34.0
4	36.492306835634984	37.0	37.0	37.0	35.0	37.0
5	36.48455771382979	37.0	37.0	37.0	35.0	37.0
6	36.47682176451891	37.0	37.0	37.0	35.0	37.0
7	36.48090296251108	37.0	37.0	37.0	35.0	37.0
8	36.43048878165376	37.0	37.0	37.0	35.0	37.0
9	38.36680900943869	39.0	39.0	39.0	37.0	39.0
10-14	38.69995768721108	39.4	39.2	39.4	38.0	39.4
15-19	39.97406350237122	41.0	40.0	41.0	38.2	41.0
20-24	39.93297484190441	41.0	40.0	41.0	38.4	41.0
25-29	39.84607794284643	41.0	40.0	41.0	38.0	41.0
30-34	39.709469466671365	41.0	40.0	41.0	38.0	41.0
35-39	39.50135066651111	41.0	40.0	41.0	37.4	41.0
40-44	39.139226863540145	41.0	39.2	41.0	35.8	41.0
45-49	38.53171403233317	40.4	37.8	41.0	35.0	41.0
50-54	37.77450783284413	39.4	36.2	41.0	34.6	41.0
55-59	36.91683346922101	38.0	35.0	40.8	34.0	41.0
60-64	36.00215412707932	36.0	35.0	39.4	33.0	41.0
65-69	35.20561750429474	35.0	35.0	37.6	33.0	40.6
70-74	34.5678196647891	35.0	35.0	36.2	32.8	39.0
75-79	34.13892136773601	35.0	35.0	35.2	32.2	37.2
80-84	33.834965503461284	35.0	35.0	35.0	32.0	36.2
85-89	33.716302155807	35.0	35.0	35.0	32.6	36.0
90-94	33.619656040512425	35.0	35.0	35.0	32.6	36.0
95-99	33.512561485611016	35.0	35.0	35.0	32.0	35.0
100-104	33.00214647676834	34.8	33.8	35.0	30.8	35.0
105-109	33.35316163471681	35.0	35.0	35.0	31.8	35.0
110-114	33.314143960650846	35.0	35.0	35.0	31.8	35.0
115-119	33.354991821078514	35.0	35.0	35.0	32.0	35.0
120-124	33.290520652247174	35.0	35.0	35.0	32.0	35.0
125-129	33.20705444893297	35.0	35.0	35.0	31.4	35.0
130-134	33.122118249994784	35.0	35.0	35.0	31.0	35.0
135-139	33.01429275509391	35.0	35.0	35.0	31.0	35.0
140-144	32.88624902104418	35.0	35.0	35.0	31.0	35.0
145-149	32.71379954269206	35.0	35.0	35.0	30.4	35.0
150-151	32.26364000667863	35.0	34.0	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	15419.0
1	3541.0
2	168635.0
3	57836.0
4	56349.0
5	56450.0
6	57647.0
7	62269.0
8	69129.0
9	72237.0
10	71881.0
11	70904.0
12	68596.0
13	65837.0
14	64747.0
15	62737.0
16	65591.0
17	67139.0
18	69065.0
19	73477.0
20	76199.0
21	82859.0
22	97048.0
23	116730.0
24	140208.0
25	164941.0
26	200188.0
27	244420.0
28	293381.0
29	363521.0
30	463020.0
31	612235.0
32	854918.0
33	1280265.0
34	2233137.0
35	6192702.0
36	2.6329686E7
37	1.6419372E7
38	990824.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.826728210362923	30.158701178782536	22.138133767193622	28.87643684366092
2	25.865190742794724	29.93748031834586	25.211233553802114	18.986095385057297
3	21.501463355050756	28.162570235633506	26.258743465978995	24.07722294333674
4	22.171467619533683	26.069172784062523	27.538773700959375	24.22058589544442
5	24.299951457866996	26.810864708116526	27.285615293146027	21.60356854087045
6	23.011432880062458	26.904984096967294	26.500270805537152	23.5833122174331
7	24.807797335463576	26.76179664482049	26.900739917768735	21.529666101947196
8	22.599204255444263	26.728974467354956	27.759471573752386	22.912349703448395
9	22.693257576970986	25.755450267655235	28.469524485015402	23.08176767035838
10-14	23.33952551787563	26.808358859078623	26.72169595278214	23.130419670263606
15-19	23.268646756474418	26.682748767809468	26.746977803671445	23.30162667204467
20-24	23.402885782640062	26.54719648058128	26.659160697976986	23.390757038801674
25-29	23.451505782316232	26.5383202313776	26.579392312741778	23.430781673564397
30-34	23.553705100234055	26.448318658204006	26.479268506300617	23.51870773526132
35-39	23.60110489794921	26.463959664181225	26.430891581469467	23.50404385640009
40-44	23.624753807729014	26.454719214310725	26.419473658263353	23.501053319696908
45-49	23.64599526147674	26.375055308035893	26.413776623244413	23.565172807242952
50-54	23.608240249237575	26.434906209800435	26.375632646929077	23.58122089403291
55-59	23.62908176754421	26.423641196579666	26.37861975491956	23.568657280956565
60-64	23.63553181693251	26.392164040756818	26.377305512204295	23.594998630106378
65-69	23.656876641061388	26.4315950594916	26.376537032312942	23.53499126713407
70-74	23.669692497311022	26.402038219372532	26.350684177824146	23.5775851054923
75-79	23.619431352697642	26.408721708610717	26.37007288863739	23.60177405005425
80-84	23.62123360105451	26.45185002673161	26.36284108689771	23.564075285316168
85-89	23.647845690477602	26.43830578222623	26.383963994532404	23.529884532763763
90-94	23.591950198934846	26.438334913393906	26.38094735333356	23.58876753433768
95-99	23.629708344746636	26.4293081408084	26.3882592660729	23.552724248372062
100-104	23.644614483631372	26.485301309652037	26.33614176173485	23.533942444981744
105-109	23.62707383660861	26.395189577447464	26.388921061452553	23.588815524491373
110-114	23.594811164966178	26.4618178432235	26.379782160872896	23.56358883093743
115-119	23.654586576429992	26.457264002333197	26.354676887674856	23.533472533561948
120-124	23.644549746017272	26.4267013512102	26.376385721843555	23.55236318092898
125-129	23.593854712814007	26.404540975716838	26.422577585180377	23.579026726288774
130-134	23.669437942050813	26.439808763080023	26.362802351006554	23.527950943862606
135-139	23.649372961511332	26.441528197527518	26.3273069945951	23.581791846366045
140-144	23.685411819361075	26.435107197429215	26.32678086188668	23.55270012132303
145-149	23.72986520826033	26.42816256233902	26.314085595356723	23.527886634043927
150-151	23.72228712431963	26.37484925998974	26.274473427532424	23.628390188158203
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	47.70316505402384
2	44.85128612785203
3	45.578686298387495
4	46.3920535149781
5	45.903519998737444
6	46.594745097495554
7	46.337463437410776
8	45.51155395889266
9	45.77502524732937
10-14	46.469945188139235
15-19	46.57027342851909
20-24	46.793642821441736
25-29	46.88228745588063
30-34	47.07241283549538
35-39	47.1051487543493
40-44	47.12580712742592
45-49	47.211168068719694
50-54	47.18946114327049
55-59	47.19773904850078
60-64	47.23053044703889
65-69	47.19186790819546
70-74	47.24727760280332
75-79	47.22120540275189
80-84	47.18530888637068
85-89	47.17773022324137
90-94	47.18071773327252
95-99	47.1824325931187
100-104	47.17855692861311
105-109	47.21588936109998
110-114	47.15839999590361
115-119	47.18805910999194
120-124	47.196912926946254
125-129	47.17288143910278
130-134	47.19738888591342
135-139	47.23116480787738
140-144	47.23811194068411
145-149	47.25775184230426
150-151	47.350677312477835
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2524.0
1	2742.5
2	2088.0
3	1098.0
4	874.0
5	709.0
6	649.0
7	619.0
8	622.0
9	629.5
10	626.5
11	661.5
12	675.0
13	699.0
14	752.5
15	1048.5
16	3301.5
17	5369.5
18	4353.0
19	4959.5
20	9894.5
21	40996.0
22	74212.5
23	94394.0
24	118059.5
25	145266.5
26	189713.5
27	230545.5
28	268572.5
29	345528.5
30	450318.0
31	554684.5
32	673623.5
33	734486.5
34	779095.5
35	891276.0
36	1023179.0
37	1125223.0
38	1175681.5
39	1274067.0
40	1433394.0
41	1589416.5
42	1755133.5
43	1961114.5
44	2155941.5
45	2304389.5
46	2443640.0
47	2638663.5
48	2876507.0
49	2996087.5
50	2946427.0
51	2868337.0
52	2853675.5
53	2802010.0
54	2641109.5
55	2408221.5
56	2119732.5
57	1779590.5
58	1452037.0
59	1169686.0
60	928274.0
61	702625.5
62	492149.5
63	339702.5
64	224100.0
65	134984.0
66	80600.5
67	48374.5
68	28927.5
69	18242.5
70	10861.0
71	5623.5
72	3501.0
73	2439.0
74	1560.5
75	1099.0
76	908.0
77	759.0
78	535.0
79	344.0
80	290.0
81	250.5
82	201.0
83	171.0
84	142.0
85	121.5
86	100.0
87	84.0
88	67.5
89	56.0
90	51.0
91	41.5
92	35.0
93	31.5
94	28.0
95	23.0
96	19.0
97	17.5
98	12.0
99	10.0
100	56.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022201640437436297
2	0.12441164284269955
3	0.05684358980236811
4	0.06219299106973314
5	0.05769723586326198
6	0.04722424751698482
7	0.04368991332498733
8	0.04075946101574643
9	0.029554287270546268
10-14	0.03380096258429968
15-19	0.03348721771943408
20-24	0.03489752996913531
25-29	0.03224934539546052
30-34	0.03303730005607719
35-39	0.03307835718125044
40-44	0.028341391364386435
45-49	0.03445616587352284
50-54	0.040533304684583764
55-59	0.03855537767936233
60-64	0.05035553759686488
65-69	0.0548307642407494
70-74	0.058885497494317866
75-79	0.05232696389060055
80-84	0.054392137286815155
85-89	0.0483796634479021
90-94	0.033919343961882564
95-99	0.03991299995175788
100-104	0.031680362069101194
105-109	0.03465358221706423
110-114	0.03977340572616882
115-119	0.04009091416084197
120-124	0.03560302823669569
125-129	0.04292111865611818
130-134	0.043555109097335155
135-139	0.04008509773477576
140-144	0.03997184849783954
145-149	0.034267303097725875
150-151	0.03295005366508403
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	5.845514E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	95.49749510633356
#Duplication Level	Relative count
1	100.0
2	34.20529069339032
3	26.02608503426424
4	23.65337852774298
5	22.024906049664725
6	21.24382875248692
7	19.821678579323557
8	18.576376096087245
9	18.01635841131825
10++	1190.1628472478078
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE