##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	H0960.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	41097372	
Filtered Sequences	0	
Sequence length	151	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.01235334463722	34.0	33.0	34.0	31.0	34.0
2	33.28258332430599	34.0	34.0	34.0	31.0	34.0
3	33.197076445666646	34.0	34.0	34.0	31.0	34.0
4	36.555370766773116	37.0	37.0	37.0	35.0	37.0
5	36.60083158115317	37.0	37.0	37.0	35.0	37.0
6	36.6059111273587	37.0	37.0	37.0	36.0	37.0
7	36.61504718598552	37.0	37.0	37.0	37.0	37.0
8	36.62460967090548	37.0	37.0	37.0	37.0	37.0
9	38.58821201511376	39.0	39.0	39.0	39.0	39.0
10-14	38.92702539228056	39.4	39.4	39.4	39.0	39.4
15-19	40.29166486849816	41.0	41.0	41.0	39.0	41.0
20-24	40.272827123836535	41.0	41.0	41.0	39.0	41.0
25-29	40.21930492781874	41.0	41.0	41.0	39.0	41.0
30-34	40.13692917396276	41.0	40.6	41.0	39.0	41.0
35-39	40.043925660258765	41.0	40.0	41.0	39.0	41.0
40-44	39.8619844938017	41.0	40.0	41.0	38.2	41.0
45-49	39.498784321294316	41.0	39.6	41.0	37.2	41.0
50-54	39.169587520097394	40.8	39.0	41.0	36.0	41.0
55-59	38.60064441590085	40.0	37.4	41.0	35.0	41.0
60-64	37.791355666245515	38.8	36.0	41.0	35.0	41.0
65-69	36.78367048384505	36.8	35.0	39.4	34.8	41.0
70-74	35.82796191445039	35.6	35.0	37.8	34.0	39.8
75-79	35.06608022040923	35.0	35.0	36.4	34.0	38.2
80-84	34.61391351252338	35.0	35.0	35.4	34.0	36.6
85-89	34.4079095860436	35.0	35.0	35.0	34.0	36.0
90-94	34.28130795808549	35.0	35.0	35.0	34.0	36.0
95-99	34.21259237695296	35.0	35.0	35.0	34.0	35.0
100-104	33.79394572966855	34.8	34.4	35.0	32.6	35.0
105-109	34.12626648730727	35.0	35.0	35.0	33.8	35.0
110-114	34.115109705798226	35.0	35.0	35.0	34.0	35.0
115-119	34.07006748266044	35.0	35.0	35.0	33.0	35.0
120-124	34.01441769561323	35.0	35.0	35.0	33.0	35.0
125-129	34.010109872718864	35.0	35.0	35.0	33.0	35.0
130-134	34.00432878287206	35.0	35.0	35.0	33.0	35.0
135-139	33.94183603759384	35.0	35.0	35.0	33.0	35.0
140-144	33.86931851506223	35.0	35.0	35.0	33.0	35.0
145-149	33.76852829908443	35.0	35.0	35.0	33.0	35.0
150-151	32.911441381215326	34.5	33.5	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	417.0
1	914.0
2	105679.0
3	24480.0
4	21874.0
5	20024.0
6	20407.0
7	21994.0
8	24042.0
9	23425.0
10	22955.0
11	21698.0
12	20073.0
13	19653.0
14	17952.0
15	17659.0
16	17834.0
17	18051.0
18	18758.0
19	19344.0
20	22330.0
21	24111.0
22	28018.0
23	34979.0
24	43199.0
25	53009.0
26	66902.0
27	80467.0
28	98551.0
29	124892.0
30	160414.0
31	214715.0
32	303633.0
33	459648.0
34	828766.0
35	2315986.0
36	1.5927709E7
37	1.945953E7
38	393280.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.703349445314643	31.385181064065364	23.222289344323556	27.689180146296437
2	23.70179461153219	30.7859533579322	26.348956611694934	19.163295418840676
3	21.503365407498475	29.42026377210442	26.8697573525182	22.206613467878903
4	21.592447462418825	27.33290346640496	28.117961753328558	22.95668731784766
5	23.20844759849938	27.675676183404914	28.06504242225086	21.050833795844852
6	22.519879843189337	28.229270256312695	27.107872718192798	22.14297718230517
7	23.62326091754782	27.874232925045977	27.628216531415852	20.87428962599035
8	21.636747689087404	27.947606701207906	28.81379330248604	21.60185230721865
9	21.54601588576828	27.086265481725256	29.499303000692436	21.868415631814024
10-14	22.242625472673698	27.95828567012204	27.923830911302428	21.875257945901833
15-19	22.199574944787475	27.83614774945469	27.87188236772812	22.092394938029713
20-24	22.259831451097316	27.706867589278357	27.854038348090764	22.179262611533563
25-29	22.380104071044745	27.666814072889018	27.735169567560835	22.2179122885054
30-34	22.37640102039588	27.605605747064754	27.711402742188106	22.30659049035126
35-39	22.436931487136185	27.640336233933084	27.658557583648125	22.264174695282602
40-44	22.500413999629554	27.59917683728636	27.639338463559348	22.261070699524733
45-49	22.497363429749186	27.56256852107773	27.603874686947204	22.336193362225877
50-54	22.497755330588845	27.582498989724158	27.59977086283434	22.319974816852657
55-59	22.48588842778796	27.58415335361243	27.560383267210398	22.369574951389215
60-64	22.486672611062744	27.549590470374646	27.538216709705253	22.425520208857357
65-69	22.518505985179047	27.550096736191264	27.555161052916294	22.3762362257134
70-74	22.50500206268317	27.537868169371755	27.543313376371326	22.41381639157375
75-79	22.46355974304361	27.53628759507591	27.544460240498804	22.455692421381677
80-84	22.513864687571004	27.566632929226564	27.52805429233493	22.391448090867495
85-89	22.48602546383468	27.56045138103393	27.535612293677115	22.417910861454278
90-94	22.47341097634427	27.515484751001846	27.529713834317516	22.48139043833637
95-99	22.49936235109646	27.526895555246213	27.553626046472544	22.42011604718478
100-104	22.50856706926635	27.537698801213562	27.53489398315814	22.41884014636196
105-109	22.463420601679847	27.51652371489476	27.5631612398248	22.45689444360059
110-114	22.47367911846013	27.578263683674713	27.5513949465058	22.39666225135936
115-119	22.538815097094854	27.525246075516467	27.528317024921062	22.407621802467617
120-124	22.52289682737423	27.486651958115694	27.52597910359464	22.464472110915437
125-129	22.50308814588056	27.54835012842388	27.55350028282534	22.395061442870215
130-134	22.529808446915272	27.557774253376117	27.495073933954277	22.417343365754334
135-139	22.494595192416153	27.528876949331842	27.484177748927458	22.492350109324548
140-144	22.49196236600259	27.57841677027337	27.523756581197382	22.405864282526654
145-149	22.528631489941628	27.605547928728136	27.466141220846364	22.39967936048387
150-151	22.43229078687974	27.557822231176175	27.436900066583014	22.572986915361067
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	45.39252959161108
2	42.86509003037287
3	43.70997887537738
4	44.549134780266485
5	44.25928139434423
6	44.6628570254945
7	44.497550543538175
8	43.23859999630606
9	43.4144315175823
10-14	44.11788341857553
15-19	44.29196988281719
20-24	44.43909406263088
25-29	44.598016359550144
30-34	44.68299151074714
35-39	44.70110618241879
40-44	44.761484699154295
45-49	44.83355679197506
50-54	44.8177301474415
55-59	44.85546337917717
60-64	44.9121928199201
65-69	44.894742210892446
70-74	44.91881845425692
75-79	44.91925216442529
80-84	44.9053127784385
85-89	44.90393632528895
90-94	44.95480141468064
95-99	44.91947839828124
100-104	44.92740721562831
105-109	44.92031504528043
110-114	44.870341369819485
115-119	44.94643689956248
120-124	44.98736893828967
125-129	44.89814958875078
130-134	44.9471518126696
135-139	44.9869453017407
140-144	44.89782664852925
145-149	44.9283108504255
150-151	45.00527770224081
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	6.5
2	13.0
3	22.0
4	35.0
5	47.0
6	60.0
7	76.5
8	111.5
9	159.0
10	189.5
11	279.0
12	411.5
13	509.5
14	1665.0
15	2968.5
16	6185.0
17	13960.0
18	16792.5
19	27537.0
20	42759.5
21	49284.5
22	52908.0
23	55196.5
24	76840.5
25	111614.0
26	138308.0
27	166126.5
28	206154.5
29	240079.0
30	271820.0
31	310579.5
32	357352.5
33	433854.5
34	536100.0
35	663119.5
36	799615.0
37	959769.0
38	1109679.0
39	1252788.0
40	1436710.5
41	1661663.5
42	1887105.5
43	2096964.5
44	2285948.0
45	2412172.0
46	2516016.5
47	2552013.0
48	2472832.5
49	2371978.5
50	2243827.0
51	1971372.5
52	1631480.5
53	1394908.5
54	1178274.0
55	929966.5
56	716639.5
57	503050.0
58	323338.5
59	211916.5
60	152153.0
61	105282.5
62	60528.0
63	31418.5
64	13638.0
65	7544.5
66	4694.0
67	3540.0
68	2847.0
69	2317.5
70	1852.5
71	1518.5
72	1350.0
73	1137.0
74	951.5
75	835.5
76	697.5
77	558.0
78	405.5
79	259.5
80	184.0
81	138.0
82	99.5
83	70.0
84	42.0
85	32.5
86	23.5
87	12.5
88	10.5
89	9.5
90	10.0
91	10.0
92	7.0
93	7.0
94	6.5
95	4.0
96	3.0
97	3.0
98	2.0
99	1.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015619003570349949
2	0.006803354725455438
3	0.005353140341917727
4	0.0022702181540951087
5	0.0070564122688915485
6	0.00909547209004021
7	0.011149131384848647
8	0.007564960601373733
9	0.008168405512644458
10-14	0.008477427705109708
15-19	0.009458025685924638
20-24	0.011100953121771387
25-29	0.008941690967490572
30-34	0.010084343105929011
35-39	0.006621834602952228
40-44	0.004683024500934025
45-49	0.007944546916527899
50-54	0.012481090031742177
55-59	0.021300632069612628
60-64	0.03672692258765353
65-69	0.052903626051806915
70-74	0.055275553872398464
75-79	0.06378850696341362
80-84	0.050495199547065925
85-89	0.02754774684863061
90-94	0.016746569586006618
95-99	0.01028094935121399
100-104	0.009240006879271989
105-109	0.010419644350981859
110-114	0.00855675151199449
115-119	0.0062991862350711865
120-124	0.0050937563598957136
125-129	0.008415136617494667
130-134	0.010802150560867979
135-139	0.007602919232889149
140-144	0.005792584499076972
145-149	0.0036537616079198444
150-151	0.0378941505067526
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	4.1097372E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	94.76139217796914
#Duplication Level	Relative count
1	100.0
2	33.52287412768157
3	25.452313259240114
4	21.271646420263632
5	18.79038511243215
6	17.342982682863788
7	16.11527526492634
8	15.074954768674075
9	15.02326182476092
10++	1029.7299043680537
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE