##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	H0961.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	45212200	
Filtered Sequences	0	
Sequence length	151	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.989604575756104	34.0	33.0	34.0	31.0	34.0
2	33.230582187993505	34.0	34.0	34.0	31.0	34.0
3	33.2779053662507	34.0	34.0	34.0	31.0	34.0
4	36.590550448772674	37.0	37.0	37.0	35.0	37.0
5	36.57212177244195	37.0	37.0	37.0	35.0	37.0
6	36.56883805698462	37.0	37.0	37.0	35.0	37.0
7	36.56727263880103	37.0	37.0	37.0	35.0	37.0
8	36.5745044479145	37.0	37.0	37.0	36.0	37.0
9	38.52421518970544	39.0	39.0	39.0	38.0	39.0
10-14	38.848301237276665	39.4	39.4	39.4	38.2	39.4
15-19	40.179977289315715	41.0	41.0	41.0	39.0	41.0
20-24	40.15563343964682	41.0	40.8	41.0	39.0	41.0
25-29	40.09850938463512	41.0	40.2	41.0	39.0	41.0
30-34	40.0127689252016	41.0	40.0	41.0	38.6	41.0
35-39	39.91027521332738	41.0	40.0	41.0	38.0	41.0
40-44	39.726307014478394	41.0	40.0	41.0	37.8	41.0
45-49	39.392495627286436	41.0	39.4	41.0	36.8	41.0
50-54	39.041976369210076	40.8	38.8	41.0	35.4	41.0
55-59	38.44372919256307	39.8	37.2	41.0	35.0	41.0
60-64	37.601425265746855	38.6	35.6	40.8	35.0	41.0
65-69	36.57390138944798	36.6	35.0	39.2	34.2	41.0
70-74	35.62984379437408	35.4	35.0	37.4	34.0	39.6
75-79	34.8979032163885	35.0	35.0	36.2	33.8	37.8
80-84	34.47030620496238	35.0	35.0	35.2	33.4	36.6
85-89	34.27354448578039	35.0	35.0	35.0	33.4	36.0
90-94	34.15028123382626	35.0	35.0	35.0	33.0	35.8
95-99	34.075403369002174	35.0	35.0	35.0	33.0	35.0
100-104	33.64185875936141	34.8	34.4	35.0	32.2	35.0
105-109	33.97874707269277	35.0	35.0	35.0	33.0	35.0
110-114	33.96605896196159	35.0	35.0	35.0	33.0	35.0
115-119	33.91513746289718	35.0	35.0	35.0	33.0	35.0
120-124	33.856904194000734	35.0	35.0	35.0	33.0	35.0
125-129	33.85013691879625	35.0	35.0	35.0	33.0	35.0
130-134	33.840441814377535	35.0	35.0	35.0	33.0	35.0
135-139	33.771061589570955	35.0	35.0	35.0	33.0	35.0
140-144	33.687216967986515	35.0	35.0	35.0	33.0	35.0
145-149	33.569005219830046	35.0	35.0	35.0	33.0	35.0
150-151	32.69386551638717	34.5	33.5	35.0	29.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	440.0
1	1068.0
2	106113.0
3	27733.0
4	22736.0
5	23145.0
6	23845.0
7	26178.0
8	29159.0
9	29538.0
10	28171.0
11	29027.0
12	27496.0
13	25569.0
14	23880.0
15	23483.0
16	23560.0
17	23260.0
18	23645.0
19	25065.0
20	28346.0
21	33365.0
22	38948.0
23	47260.0
24	59746.0
25	73799.0
26	92488.0
27	114416.0
28	142553.0
29	180003.0
30	232077.0
31	311465.0
32	436083.0
33	653253.0
34	1144203.0
35	2989439.0
36	1.7801405E7
37	1.9881014E7
38	409226.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.860512908998803	31.500797479919214	22.8766859344894	27.762003676592585
2	23.60373977177013	30.622864629218533	26.091638825829396	19.681756773181945
3	21.690919994046407	28.99508917040829	26.713285451102465	22.600705384442836
4	21.737625707922685	27.069199652333566	27.806024495903138	23.387150143840618
5	23.39650615569936	27.51253633181607	27.658522756907818	21.432434755576754
6	22.843225534905127	27.84811579774326	26.87794454298605	22.43071412436557
7	23.80958901173347	27.621887317480176	27.37216906923695	21.19635460154941
8	21.825934628071177	27.715996740999003	28.495147840872026	21.96292079005779
9	21.764697476887072	26.73693090516825	29.182965712910253	22.315405905034428
10-14	22.60019966080959	27.567181579158635	27.573001727282463	22.25961703274931
15-19	22.507293665354354	27.483790655373607	27.50451884654177	22.504396832730265
20-24	22.63809653990925	27.35967970162519	27.462567185963486	22.539656572502075
25-29	22.67287420237506	27.375476234684275	27.356836285602387	22.59481327733828
30-34	22.730248949644746	27.281874426333957	27.303873773763776	22.68400285025752
35-39	22.786070950729144	27.27784041701376	27.24238047846064	22.693708153796454
40-44	22.851234941830484	27.23254872676773	27.260811348706138	22.65540498269565
45-49	22.828693892439514	27.16255740638383	27.270042207793843	22.738706493382807
50-54	22.89015318135456	27.218110736218353	27.21900218869944	22.672733893727646
55-59	22.81648297399099	27.280827537571987	27.20185708475321	22.70083240368381
60-64	22.89034358042599	27.202080561413716	27.1434874203876	22.76408843777269
65-69	22.83299125764614	27.187861679785957	27.2123143618214	22.766832700746505
70-74	22.819418263626776	27.259106965200168	27.111265949251102	22.81020882192196
75-79	22.839046277870796	27.220257336815557	27.198338887963452	22.74235749735019
80-84	22.842432686227912	27.247461281797104	27.14277857381349	22.767327458161493
85-89	22.833187135528252	27.232826101543807	27.1268481979617	22.80713856496624
90-94	22.771289242446176	27.20617615694182	27.18003463099601	22.842499969615996
95-99	22.882500519049458	27.17779858350485	27.21695081702787	22.72275008041783
100-104	22.84592377711752	27.177655437974146	27.135071415776927	22.841349369131407
105-109	22.826284249829744	27.17052955076517	27.15680349928752	22.846382700117566
110-114	22.81335531068783	27.196804537426967	27.210077305846553	22.77976284603865
115-119	22.834715440880863	27.206835886719787	27.140765093074986	22.817683579324363
120-124	22.81189681706071	27.233042279822634	27.136589855550476	22.818471047566184
125-129	22.845411148422297	27.227417843200275	27.165758022242343	22.761412986135092
130-134	22.88415075455197	27.21337361718406	27.09661538949411	22.80586023876986
135-139	22.80932045930064	27.249945279399075	27.13468971646387	22.806044544836414
140-144	22.871868423597153	27.19770366904804	27.164190028046796	22.766237879308008
145-149	22.921589047705808	27.195820833994073	27.129178040495283	22.753412077804832
150-151	22.852264309430836	27.184642544718663	27.09998041952574	22.863112726324765
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	45.622516585591384
2	43.285496544952075
3	44.29162537848924
4	45.124775851763296
5	44.82894091127611
6	45.273939659270695
7	45.00594361328288
8	43.78885541812897
9	44.0801033819215
10-14	44.8598166935589
15-19	45.01169049808462
20-24	45.177753112411324
25-29	45.26768747971334
30-34	45.41425179990227
35-39	45.479779104525605
40-44	45.506639924526134
45-49	45.567400385822324
50-54	45.56288707508221
55-59	45.5173153776748
60-64	45.65443201819868
65-69	45.59982395839264
70-74	45.629627085548734
75-79	45.58140377522099
80-84	45.609760144389405
85-89	45.64032570049449
90-94	45.61378921206217
95-99	45.60525059946728
100-104	45.68727314624893
105-109	45.67266694994731
110-114	45.59311815672648
115-119	45.65239902020522
120-124	45.63036786462689
125-129	45.606824134557385
130-134	45.69001099332183
135-139	45.615365004137054
140-144	45.63810630290516
145-149	45.67500112551065
150-151	45.7153770357556
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14.0
1	18.0
2	24.5
3	29.0
4	41.5
5	58.5
6	76.5
7	97.0
8	108.5
9	134.0
10	173.5
11	375.5
12	744.0
13	1243.5
14	2491.5
15	4330.5
16	4817.5
17	5547.5
18	7534.0
19	10793.0
20	18073.5
21	27626.5
22	41152.5
23	63293.0
24	78025.5
25	87099.0
26	100852.5
27	124683.5
28	165512.0
29	222017.5
30	280013.5
31	319455.5
32	358936.5
33	461198.0
34	599566.0
35	668805.0
36	748240.0
37	859364.5
38	988971.5
39	1164984.5
40	1333058.0
41	1560014.0
42	1856475.0
43	2124717.0
44	2397273.0
45	2736580.5
46	3072722.0
47	3201523.0
48	3081633.5
49	2851362.0
50	2608567.0
51	2278659.5
52	1886294.5
53	1546368.0
54	1275035.5
55	1051983.5
56	826825.0
57	646471.0
58	498742.5
59	347739.0
60	222185.0
61	140340.5
62	89461.0
63	54099.5
64	35747.5
65	32778.0
66	19955.5
67	9482.0
68	5153.5
69	1641.5
70	799.0
71	581.5
72	351.0
73	252.0
74	175.5
75	108.0
76	64.5
77	57.0
78	52.0
79	42.0
80	41.5
81	38.0
82	30.0
83	21.5
84	17.5
85	18.0
86	18.5
87	16.5
88	14.0
89	10.5
90	10.0
91	9.5
92	7.0
93	9.0
94	6.0
95	5.0
96	7.0
97	3.5
98	4.0
99	6.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016146084463927877
2	0.0069251219803504365
3	0.005589199375389829
4	0.0023179584271501943
5	0.007082159240205077
6	0.008838322399706274
7	0.010729404895138923
8	0.007197172444605659
9	0.007789932805747121
10-14	0.008246889114000204
15-19	0.009296605783394748
20-24	0.010953238285241595
25-29	0.008674649762674676
30-34	0.009942891520430326
35-39	0.006351825392261381
40-44	0.0043289200702465265
45-49	0.007085698108032787
50-54	0.011234135919066093
55-59	0.01982694936322497
60-64	0.03491004640340439
65-69	0.0506124453134331
70-74	0.05262517639044329
75-79	0.0606765430569625
80-84	0.048078615948792584
85-89	0.02659237993284998
90-94	0.01657472982955928
95-99	0.010090196893758764
100-104	0.009210345880094312
105-109	0.01047150990219454
110-114	0.00850876533325076
115-119	0.006224426150463813
120-124	0.005189307310858573
125-129	0.00847116486258134
130-134	0.010917849606964493
135-139	0.0076293566780647705
140-144	0.005638743524977771
145-149	0.003561428110111872
150-151	0.036860626114190415
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	4.52122E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	95.14848796221376
#Duplication Level	Relative count
1	100.0
2	37.3730378578024
3	29.010849492151433
4	25.3808864265928
5	23.020544783010155
6	20.290858725761773
7	19.067405355494
8	16.874422899353647
9	16.441597414589104
10++	866.7416897506926
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE