##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	H0963.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	39355366	
Filtered Sequences	0	
Sequence length	151	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.032982338418606	34.0	33.0	34.0	31.0	34.0
2	33.19790437217634	34.0	34.0	34.0	31.0	34.0
3	33.28698579502475	34.0	34.0	34.0	31.0	34.0
4	36.62389189824839	37.0	37.0	37.0	35.0	37.0
5	36.5807190053829	37.0	37.0	37.0	35.0	37.0
6	36.597656898934694	37.0	37.0	37.0	36.0	37.0
7	36.613781282074726	37.0	37.0	37.0	36.0	37.0
8	36.59260391581672	37.0	37.0	37.0	36.0	37.0
9	38.57502389890111	39.0	39.0	39.0	39.0	39.0
10-14	38.91254649747127	39.4	39.4	39.4	38.8	39.4
15-19	40.25804997468451	41.0	41.0	41.0	39.0	41.0
20-24	40.22109050135629	41.0	41.0	41.0	39.0	41.0
25-29	40.1481589524539	41.0	40.6	41.0	39.0	41.0
30-34	40.035353395010986	41.0	40.0	41.0	39.0	41.0
35-39	39.88912992957555	41.0	40.0	41.0	38.0	41.0
40-44	39.62186035825458	41.0	40.0	41.0	37.6	41.0
45-49	39.13712023920702	41.0	39.0	41.0	35.8	41.0
50-54	38.607970409930886	40.0	37.6	41.0	35.0	41.0
55-59	37.856219164624214	39.2	36.0	41.0	35.0	41.0
60-64	36.967010577414015	37.4	35.0	40.2	34.8	41.0
65-69	36.01510056341491	35.8	35.0	38.6	34.0	40.8
70-74	35.20316165272099	35.0	35.0	36.6	34.0	39.0
75-79	34.59777964204424	35.0	35.0	35.8	33.2	37.2
80-84	34.26739539406138	35.0	35.0	35.0	33.0	36.2
85-89	34.130062558686404	35.0	35.0	35.0	33.0	36.0
90-94	34.02330859786694	35.0	35.0	35.0	33.0	35.2
95-99	33.9486874750447	35.0	35.0	35.0	33.0	35.0
100-104	33.49111401479534	34.8	34.4	35.0	32.0	35.0
105-109	33.83001002201326	35.0	35.0	35.0	33.0	35.0
110-114	33.80127554143443	35.0	35.0	35.0	33.0	35.0
115-119	33.73513479203827	35.0	35.0	35.0	33.0	35.0
120-124	33.65900403518036	35.0	35.0	35.0	33.0	35.0
125-129	33.642118673219805	35.0	35.0	35.0	33.0	35.0
130-134	33.625027758603494	35.0	35.0	35.0	33.0	35.0
135-139	33.53857919654463	35.0	35.0	35.0	33.0	35.0
140-144	33.438572442700696	35.0	35.0	35.0	32.4	35.0
145-149	33.29955985163498	35.0	35.0	35.0	32.0	35.0
150-151	32.378057289570116	34.5	33.5	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	357.0
1	886.0
2	86862.0
3	25636.0
4	24784.0
5	23637.0
6	23180.0
7	26222.0
8	29963.0
9	30811.0
10	30161.0
11	31347.0
12	29668.0
13	29063.0
14	27533.0
15	28466.0
16	29410.0
17	30080.0
18	31700.0
19	33391.0
20	35861.0
21	38434.0
22	45106.0
23	53470.0
24	62355.0
25	71650.0
26	88249.0
27	105898.0
28	129169.0
29	161846.0
30	206024.0
31	273418.0
32	383237.0
33	582362.0
34	1064887.0
35	3228311.0
36	1.7937322E7
37	1.4133751E7
38	180859.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.849989067080955	31.074070094496815	22.122042482751553	27.953898355670674
2	24.474360001884495	30.850922175747357	25.74154897756174	18.933168844806406
3	20.522722278915268	28.84455867925924	27.071652965680165	23.561066076145327
4	21.89589684102825	26.461318269636525	28.16263397488921	23.48015091444601
5	23.889833101070096	27.216732385714913	27.755380143822194	21.138054369392798
6	22.527042244895444	27.582712428677127	26.939637280123137	22.950608046304296
7	24.286905429671684	27.350736358834144	27.350649957242645	21.011708254251523
8	22.260762157098718	27.159673010204195	28.368298841576767	22.211265991120317
9	22.168049178332446	26.328267046212556	28.993169139585017	22.51051463586998
10-14	22.795867721106863	27.390984236873216	27.268895250574122	22.5442527914458
15-19	22.74986068090221	27.201791021846304	27.3142073755616	22.73414092168989
20-24	22.88613025596707	27.095187405504156	27.238601551194435	22.780080787334335
25-29	22.920372584578075	27.126266049914104	27.14691459558623	22.806446769921592
30-34	22.942694891050216	27.00046635583359	27.07845119161237	22.978387561503823
35-39	23.005958650538066	27.013481354727116	27.037541607511727	22.94301838722309
40-44	23.05778702352952	27.01379055271537	27.023899395028657	22.90452302872645
45-49	23.07797792429096	26.936102540546834	27.00326846697052	22.982651068191686
50-54	23.05528286790028	26.966799597197294	27.019722190716344	22.95819534418608
55-59	23.09812218397907	26.978297171440573	26.932813919564648	22.990766725015714
60-64	23.067455407099153	26.94356968375452	26.9448939960925	23.044080913053826
65-69	23.08162131742268	26.97512885797646	26.945767622021876	22.997482202578983
70-74	23.089154679359453	26.98165620606957	26.958170118200407	22.971018996370567
75-79	23.073582563304363	26.93685077213166	26.940174896110957	23.049391768453027
80-84	23.06621726911165	26.942735135067363	27.00115367165437	22.989893924166623
85-89	23.099966472171168	26.99356370097109	26.930754112021194	22.975715714836547
90-94	23.01732480303931	26.969651662356632	26.992410699509428	23.020612835094635
95-99	23.046790937688165	27.018854521151187	26.962437199040483	22.97191734212016
100-104	23.048778183385622	27.02783645567321	26.93485202299169	22.988533337949477
105-109	23.014017127079807	27.001398638347496	26.9468555495335	23.0377286850392
110-114	23.04133875240498	26.998792595941694	26.947983526359128	23.0118851252942
115-119	23.02958040144794	27.033086822364616	26.928331773263846	23.009001002923593
120-124	23.052384760704484	26.951367901149435	26.92292868058015	23.073318657565927
125-129	23.063537209223774	27.002739871451524	26.93233187670161	23.001391042623094
130-134	23.060719008854825	27.005361249614275	26.92817659649402	23.005743145036885
135-139	23.03657452906535	26.960488420290314	26.96290961933333	23.040027431311007
140-144	23.06593359396101	27.042004652954105	26.8910042875399	23.00105746554498
145-149	23.080634086709875	27.04341251781388	26.903947658239787	22.972005737236458
150-151	23.09349444474128	26.969077106727656	26.81743209464064	23.11999635389042
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	46.803887422751636
2	43.4075288466909
3	44.08378835506059
4	45.37604775547426
5	45.02788747046289
6	45.47765029119974
7	45.29861368392321
8	44.47202814821903
9	44.67856381420243
10-14	45.34012051255266
15-19	45.484001602592095
20-24	45.66621104330141
25-29	45.72681935449967
30-34	45.92108245255404
35-39	45.94897703776116
40-44	45.96231005225597
45-49	46.06062899248264
50-54	46.01347821208636
55-59	46.08888890899478
60-64	46.11153632015298
65-69	46.07910352000167
70-74	46.06017367573002
75-79	46.12297433175739
80-84	46.05611119327827
85-89	46.075682187007715
90-94	46.03793763813394
95-99	46.01870827980833
100-104	46.0373115213351
105-109	46.051745812119
110-114	46.05322387769918
115-119	46.038581404371534
120-124	46.12570341827041
125-129	46.06492825184687
130-134	46.066462153891706
135-139	46.07660196037636
140-144	46.06699105950599
145-149	46.05263982394633
150-151	46.2134907986317
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	5.0
2	6.5
3	17.5
4	28.0
5	41.0
6	65.0
7	75.0
8	76.5
9	85.0
10	96.5
11	119.0
12	138.0
13	168.0
14	251.5
15	1245.0
16	2608.0
17	3541.5
18	4700.5
19	5122.0
20	5536.0
21	7915.5
22	15640.0
23	35300.0
24	51883.5
25	63830.5
26	84762.0
27	105175.0
28	122646.5
29	131686.0
30	157360.0
31	208992.0
32	263538.0
33	343408.0
34	434894.0
35	515392.0
36	619308.5
37	770443.0
38	911542.0
39	1036004.5
40	1229679.5
41	1437133.5
42	1634253.5
43	1919124.0
44	2180808.5
45	2307914.5
46	2375660.0
47	2367933.5
48	2326045.5
49	2337780.0
50	2295171.5
51	2136837.5
52	1901753.0
53	1641377.0
54	1413486.5
55	1148461.0
56	867839.0
57	642544.5
58	455577.5
59	310164.5
60	200742.5
61	128152.0
62	82072.0
63	48832.0
64	25070.5
65	13873.0
66	8253.0
67	4453.5
68	2769.5
69	1907.5
70	1331.0
71	1011.0
72	780.5
73	620.5
74	544.5
75	410.0
76	324.5
77	250.0
78	190.5
79	150.0
80	101.5
81	74.0
82	49.5
83	36.0
84	26.0
85	18.0
86	17.0
87	15.0
88	9.5
89	10.5
90	14.5
91	9.5
92	5.0
93	4.0
94	4.0
95	4.5
96	3.0
97	2.0
98	2.5
99	6.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01604355553446003
2	0.006789417229660626
3	0.005379190222751327
4	0.002243658463244885
5	0.006979988446810531
6	0.008921273912177567
7	0.010771085193312647
8	0.007173100613522435
9	0.007815960852708116
10-14	0.008257069696671096
15-19	0.00919366370522383
20-24	0.010917443888083776
25-29	0.008745948392399654
30-34	0.009831950235197914
35-39	0.0063422101067488485
40-44	0.004320630635222653
45-49	0.007551702098260248
50-54	0.011908922407175683
55-59	0.020671641066684527
60-64	0.036065221703185275
65-69	0.05211385913676931
70-74	0.054482532318464526
75-79	0.0627690770300548
80-84	0.04971062904103089
85-89	0.02728115906735564
90-94	0.016773316248666063
95-99	0.01010484821815658
100-104	0.009192139135486633
105-109	0.010399090177436033
110-114	0.008478132308564987
115-119	0.006144016040912947
120-124	0.004997539598539116
125-129	0.008387166314245431
130-134	0.010872723175792597
135-139	0.007529341742114658
140-144	0.005631252419301602
145-149	0.0034724616714274744
150-151	0.03761621731582931
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	3.9355366E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	95.06666082882725
#Duplication Level	Relative count
1	100.0
2	32.830411951778714
3	26.21847496486946
4	22.927298276754676
5	21.470305450780266
6	20.02070852747578
7	18.65986243621034
8	16.966200724798462
9	15.132016862658087
10++	1204.955254788847
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE