##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	H0964.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	50072602	
Filtered Sequences	0	
Sequence length	151	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.003655991354314	34.0	33.0	34.0	31.0	34.0
2	33.19470448130497	34.0	34.0	34.0	31.0	34.0
3	33.20213958523665	34.0	34.0	34.0	31.0	34.0
4	36.55618505704976	37.0	37.0	37.0	35.0	37.0
5	36.52234305698753	37.0	37.0	37.0	35.0	37.0
6	36.49566227854506	37.0	37.0	37.0	35.0	37.0
7	36.53005755522751	37.0	37.0	37.0	35.0	37.0
8	36.50327009169605	37.0	37.0	37.0	35.0	37.0
9	38.461522131404315	39.0	39.0	39.0	38.0	39.0
10-14	38.774102695921414	39.4	39.2	39.4	38.2	39.4
15-19	40.112417221697406	41.0	40.4	41.0	39.0	41.0
20-24	40.08214486636824	41.0	40.0	41.0	39.0	41.0
25-29	40.0252803718888	41.0	40.0	41.0	39.0	41.0
30-34	39.91760004403206	41.0	40.0	41.0	38.2	41.0
35-39	39.809213166114276	41.0	40.0	41.0	38.0	41.0
40-44	39.63185763743614	41.0	40.0	41.0	37.8	41.0
45-49	39.32505649696415	41.0	39.4	41.0	36.6	41.0
50-54	38.983035796701756	41.0	39.0	41.0	35.4	41.0
55-59	38.44843576133711	40.0	37.4	41.0	35.0	41.0
60-64	37.72160048722853	39.0	36.0	41.0	35.0	41.0
65-69	36.800282981100125	37.2	35.0	39.8	34.4	41.0
70-74	35.87898118815555	35.8	35.0	38.2	34.0	40.4
75-79	35.09146042380622	35.0	35.0	36.6	34.0	38.8
80-84	34.56911285736659	35.0	35.0	36.0	33.8	37.0
85-89	34.28785538247043	35.0	35.0	35.0	34.0	36.0
90-94	34.11008104591808	35.0	35.0	35.0	33.2	36.0
95-99	34.01264607738979	35.0	35.0	35.0	33.0	36.0
100-104	33.5712136868781	34.8	34.4	35.0	32.2	35.2
105-109	33.900961539805735	35.0	35.0	35.0	33.0	35.0
110-114	33.88404416850556	35.0	35.0	35.0	33.0	35.0
115-119	33.83195930980379	35.0	35.0	35.0	33.0	35.0
120-124	33.77009445604604	35.0	35.0	35.0	33.0	35.0
125-129	33.75768988797507	35.0	35.0	35.0	33.0	35.0
130-134	33.74566374241946	35.0	35.0	35.0	33.0	35.0
135-139	33.67397776133144	35.0	35.0	35.0	33.0	35.0
140-144	33.5944620732911	35.0	35.0	35.0	33.0	35.0
145-149	33.47983344664214	35.0	35.0	35.0	33.0	35.0
150-151	32.61598066144036	34.5	33.5	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	492.0
1	1223.0
2	139094.0
3	37964.0
4	32669.0
5	31456.0
6	33008.0
7	36223.0
8	40271.0
9	39246.0
10	38766.0
11	39139.0
12	35952.0
13	34849.0
14	33009.0
15	32071.0
16	32761.0
17	33753.0
18	34715.0
19	37161.0
20	40095.0
21	45572.0
22	51717.0
23	61851.0
24	76111.0
25	92241.0
26	111420.0
27	135079.0
28	164696.0
29	205198.0
30	262845.0
31	345029.0
32	481301.0
33	719408.0
34	1250258.0
35	3152131.0
36	1.7034405E7
37	2.4230508E7
38	868915.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.109341126572225	33.13295486093335	23.819822124590125	25.93788188790429
2	22.553269126739934	32.655035475469354	27.52453833556641	17.2671570622243
3	18.637252103519618	30.86639971182682	28.738643973907514	21.757704210746052
4	19.895685118123644	28.43644410315454	30.23226697436015	21.435603804361662
5	21.984915045898074	29.146444528306333	29.588092215367585	19.28054821042801
6	20.730829683773326	29.5033332177575	28.74308522290504	21.02275187556413
7	22.199514496022132	29.363804997164905	29.14110208272494	19.29557842408802
8	20.32492157573333	29.067048566273684	30.2005522178841	20.407477640108887
9	20.41873532763635	28.005213644793887	30.99972597607044	20.57632505149932
10-14	20.89861231679295	29.26139850678135	29.14476666441375	20.695222512011945
15-19	20.822266874598448	29.110308766971343	29.206480852181254	20.86094350624895
20-24	21.02113690511593	28.987818075115584	29.080537622858945	20.91050739690954
25-29	21.026778236457027	29.023272845217917	28.976305753982373	20.973643164342683
30-34	21.094141717143373	28.88618988106576	28.968394313381395	21.05127408840947
35-39	21.131547332414574	28.903826960567418	28.890093225902685	21.074532481115327
40-44	21.151790617666528	28.89940369091768	28.8897073452341	21.059098346181692
45-49	21.143218808700055	28.825432420502505	28.91412970907031	21.117219061727134
50-54	21.15360964672855	28.884345048076472	28.86641500154284	21.09563030365214
55-59	21.152360564607548	28.876819361884976	28.889762383728936	21.081057689778547
60-64	21.132984635342705	28.854167708436467	28.86890588341544	21.143941772805384
65-69	21.217638799215276	28.850923424566936	28.857755369881644	21.073682406336143
70-74	21.148511362111545	28.86810510202198	28.862999414224632	21.120384121641845
75-79	21.1340213684408	28.875308376361907	28.83239370845238	21.158276546744915
80-84	21.17414932450964	28.810320308078435	28.89488205670298	21.120648310708944
85-89	21.153096956499194	28.86464702944021	28.88618432299575	21.096071691064846
90-94	21.17179650032621	28.80099369170452	28.89813157161385	21.12907823635542
95-99	21.151294845388993	28.851233739908277	28.875206566044948	21.122264848657778
100-104	21.145727481378213	28.875283160117444	28.88479942522925	21.094189933275096
105-109	21.134944353279504	28.83796554846069	28.866421606199378	21.160668492060427
110-114	21.142975678099823	28.86265782743298	28.865323386705505	21.129043107761696
115-119	21.15307547908128	28.88001751517048	28.88168240065334	21.085224605094897
120-124	21.094626290380166	28.857461272507578	28.905682476794947	21.14222996031731
125-129	21.161838095938318	28.861740443149987	28.895636864980958	21.08078459593074
130-134	21.14359682766962	28.887520754158984	28.86899804638142	21.099884371789976
135-139	21.15456507145663	28.849959097929677	28.86283736219019	21.132638468423504
140-144	21.151835176105234	28.923401011030432	28.826839788866852	21.09792402399748
145-149	21.158247073068477	28.972886754359024	28.84405925963301	21.024806912939486
150-151	21.175790910081204	28.86338530507097	28.78479213460951	21.176031650238315
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.04722301447652
2	39.820426188964234
3	40.39495631426567
4	41.33128892248531
5	41.265463256326086
6	41.75358155933746
7	41.495092920110146
8	40.73239921584222
9	40.99506037913567
10-14	41.5938348288049
15-19	41.6832103808474
20-24	41.931644302025475
25-29	42.00042140079971
30-34	42.145415805552844
35-39	42.206079813529904
40-44	42.21088896384822
45-49	42.26043787042719
50-54	42.24923995038069
55-59	42.233418254386095
60-64	42.27692640814809
65-69	42.29132120555142
70-74	42.26889548375339
75-79	42.29229791518571
80-84	42.294797635218586
85-89	42.24916864756404
90-94	42.300874736681635
95-99	42.27355969404677
100-104	42.239917414653306
105-109	42.29561284533993
110-114	42.27201878586152
115-119	42.23830008417618
120-124	42.23685625069747
125-129	42.24262269186906
130-134	42.243481199459595
135-139	42.28720353988014
140-144	42.249759200102716
145-149	42.18305398600797
150-151	42.35182256031952
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	11.0
2	18.0
3	33.0
4	55.0
5	74.0
6	103.0
7	136.5
8	156.0
9	169.5
10	191.0
11	231.0
12	255.0
13	273.0
14	324.0
15	403.5
16	587.0
17	2631.5
18	5206.5
19	10431.5
20	17333.0
21	22528.0
22	40513.0
23	68354.5
24	113155.0
25	166905.5
26	218654.5
27	339388.5
28	540679.0
29	707272.0
30	786060.5
31	839077.5
32	912286.0
33	1018415.5
34	1180883.5
35	1331396.0
36	1521880.0
37	1724745.5
38	1920186.5
39	2250205.0
40	2500226.0
41	2584751.5
42	2662755.0
43	2769116.5
44	2879964.5
45	2887726.0
46	2755882.5
47	2623754.5
48	2451601.5
49	2164040.5
50	1843845.0
51	1528540.0
52	1230597.5
53	972600.0
54	756302.0
55	573912.0
56	410571.0
57	266290.0
58	174914.5
59	114117.0
60	68714.0
61	43182.0
62	26744.5
63	15819.5
64	9388.5
65	5486.5
66	3325.0
67	2222.0
68	1443.5
69	983.0
70	693.0
71	472.5
72	332.5
73	206.0
74	136.5
75	115.5
76	93.5
77	75.0
78	59.5
79	52.0
80	46.0
81	34.5
82	24.5
83	27.0
84	25.0
85	17.0
86	14.0
87	14.5
88	13.5
89	10.0
90	9.5
91	11.0
92	10.0
93	10.0
94	10.5
95	5.0
96	1.5
97	5.5
98	7.5
99	7.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01599477494698598
2	0.0069838591571494526
3	0.005412141354267949
4	0.0022507318473284055
5	0.007003830158456715
6	0.008919049183823122
7	0.010890187012849861
8	0.007183569170222071
9	0.00795245272055165
10-14	0.008216069937807506
15-19	0.009272935326987801
20-24	0.010963680297660585
25-29	0.008694175709103354
30-34	0.0099563429917223
35-39	0.0063735453571995325
40-44	0.004378442326604078
45-49	0.007295806197568882
50-54	0.011593166258865478
55-59	0.020179498560909618
60-64	0.03506468467526413
65-69	0.05065245061560811
70-74	0.052834082798413395
75-79	0.0609203412277237
80-84	0.048339009824174904
85-89	0.026735978290083666
90-94	0.01657912644523646
95-99	0.010145268664088996
100-104	0.009266544606569477
105-109	0.01044683078382865
110-114	0.008607501563429838
115-119	0.0061754330242314946
120-124	0.005018313208488746
125-129	0.008368648387794986
130-134	0.01080071692699333
135-139	0.007474746369281947
140-144	0.00561824208775889
145-149	0.003532470711228468
150-151	0.03721496238601701
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	5.0072602E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	95.35418294139302
#Duplication Level	Relative count
1	100.0
2	30.337585362710993
3	21.704677232315422
4	19.15345960572091
5	16.84705579177941
6	15.461925009663704
7	14.38603272774127
8	13.767555727354722
9	12.891379976807112
10++	1043.9440793712151
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE