##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	H0965.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	59113878	
Filtered Sequences	0	
Sequence length	151	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.00632738051799	34.0	33.0	34.0	31.0	34.0
2	33.16121576391926	34.0	33.0	34.0	31.0	34.0
3	33.326465233764566	34.0	34.0	34.0	31.0	34.0
4	36.62566783387143	37.0	37.0	37.0	35.0	37.0
5	36.595104469376885	37.0	37.0	37.0	35.0	37.0
6	36.579183047337885	37.0	37.0	37.0	35.0	37.0
7	36.6332049303211	37.0	37.0	37.0	36.0	37.0
8	36.63914285914384	37.0	37.0	37.0	36.0	37.0
9	38.609219361314786	39.0	39.0	39.0	39.0	39.0
10-14	38.954532497428104	39.4	39.4	39.4	39.0	39.4
15-19	40.317714956883734	41.0	41.0	41.0	39.0	41.0
20-24	40.28909538974926	41.0	41.0	41.0	39.0	41.0
25-29	40.22713270816034	41.0	41.0	41.0	39.0	41.0
30-34	40.130808992094885	41.0	40.2	41.0	39.0	41.0
35-39	40.00446167987828	41.0	40.0	41.0	38.4	41.0
40-44	39.7708462808006	41.0	40.0	41.0	37.8	41.0
45-49	39.33743645104793	41.0	39.2	41.0	36.4	41.0
50-54	38.86468622139796	40.4	38.2	41.0	35.2	41.0
55-59	38.13914516655463	39.4	36.6	41.0	35.0	41.0
60-64	37.22329594076031	37.8	35.2	40.4	35.0	41.0
65-69	36.21857780672079	36.2	35.0	38.6	34.2	40.8
70-74	35.3561542113681	35.0	35.0	36.8	34.0	39.0
75-79	34.731399266344866	35.0	35.0	35.8	34.0	37.0
80-84	34.39775169884811	35.0	35.0	35.0	34.0	36.0
85-89	34.26299365438349	35.0	35.0	35.0	34.0	36.0
90-94	34.16710739904427	35.0	35.0	35.0	34.0	35.2
95-99	34.10426920054205	35.0	35.0	35.0	33.6	35.0
100-104	33.6671487937232	34.8	34.4	35.0	32.2	35.0
105-109	34.002044741507234	35.0	35.0	35.0	33.0	35.0
110-114	33.98035301625787	35.0	35.0	35.0	33.0	35.0
115-119	33.92539017656733	35.0	35.0	35.0	33.0	35.0
120-124	33.858918029366976	35.0	35.0	35.0	33.0	35.0
125-129	33.849252431045045	35.0	35.0	35.0	33.0	35.0
130-134	33.837636891289726	35.0	35.0	35.0	33.0	35.0
135-139	33.76325432075358	35.0	35.0	35.0	33.0	35.0
140-144	33.67544592828101	35.0	35.0	35.0	33.0	35.0
145-149	33.55795458047939	35.0	35.0	35.0	33.0	35.0
150-151	32.67770613864988	34.5	33.5	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	582.0
1	1357.0
2	122110.0
3	34542.0
4	30583.0
5	28986.0
6	28479.0
7	32632.0
8	37374.0
9	37601.0
10	39179.0
11	40282.0
12	37772.0
13	36421.0
14	35424.0
15	35349.0
16	34859.0
17	35269.0
18	36845.0
19	39251.0
20	41152.0
21	47443.0
22	53516.0
23	60659.0
24	74621.0
25	85509.0
26	104177.0
27	127793.0
28	156073.0
29	200141.0
30	260898.0
31	350538.0
32	498634.0
33	765714.0
34	1414850.0
35	4242176.0
36	2.6820272E7
37	2.2826542E7
38	258273.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.255917575810816	31.59106250589106	22.112865816057646	28.040154102240482
2	24.276328385348435	31.269926585930683	25.85329566489556	18.600449363825316
3	20.262920164511364	29.179050168813305	27.24101766778857	23.317011998886763
4	21.608869765892823	26.59449540495984	28.553083177392086	23.24355165175525
5	23.62586631805349	27.545133837893992	28.090713186860444	20.73828665719207
6	22.17906077811022	27.965885215928175	27.17046244751628	22.684591558445327
7	24.056680057693534	27.656897160463128	27.565032323906287	20.72139045793705
8	21.939746390433445	27.47333208807427	28.57486867470978	22.012052846782503
9	21.917849447768937	26.436208657700856	29.360376012275868	22.28556588225434
10-14	22.575509418192855	27.585382795915294	27.56726912646445	22.271838659427402
15-19	22.445957705063364	27.484943949063695	27.57633406498052	22.492764280892416
20-24	22.603906470221684	27.37492780127313	27.481836343443767	22.539329385061425
25-29	22.662523957683938	27.374153385595307	27.4290332953334	22.53428936138735
30-34	22.709154639468412	27.229899057847962	27.37172096766286	22.68922533502076
35-39	22.734548983690534	27.283799836841325	27.30439023834357	22.677260941124572
40-44	22.769939834752204	27.256797692680752	27.31576780519382	22.657494667373225
45-49	22.77538393992533	27.211880742697154	27.303699609867344	22.709035707510168
50-54	22.75526960952669	27.277416177661152	27.288096462830584	22.679217749981575
55-59	22.787090647722234	27.234770634768847	27.2788644239684	22.699274293540515
60-64	22.772455015523065	27.21131452155611	27.267767628724666	22.748462834196165
65-69	22.79723584770146	27.240235924491557	27.251588062997527	22.71094016480945
70-74	22.804969643539945	27.24114047733429	27.280625040970722	22.673264838155045
75-79	22.75384314671163	27.23518048935662	27.268414099290716	22.742562264641037
80-84	22.76711016279258	27.248052985633592	27.26139112675457	22.72344572481926
85-89	22.76499992468439	27.274218509834718	27.255130545644057	22.705651019836832
90-94	22.741569655015972	27.26440472384396	27.261455687466384	22.732569933673684
95-99	22.726208034339017	27.29286334224062	27.28638705620916	22.69454156721121
100-104	22.75454949974583	27.294021911540174	27.252956366659078	22.69847222205492
105-109	22.758581644643655	27.21177367188365	27.278445490950137	22.75119919252256
110-114	22.77665092074454	27.24218094654491	27.256574730460407	22.724593402250143
115-119	22.755384830195936	27.291887727667465	27.25973354382202	22.692993898314587
120-124	22.762526043990423	27.237945443027556	27.275352448218925	22.724176064763093
125-129	22.743794051849758	27.254032502078324	27.275796738348884	22.726376707723038
130-134	22.767237179185468	27.264254288944144	27.25108504956686	22.71742348230353
135-139	22.71572022635628	27.26748144057757	27.26196963028394	22.754828702782216
140-144	22.764502282862896	27.28865475750284	27.242108047024544	22.704734912609727
145-149	22.802908787429672	27.299952654422782	27.220658438414468	22.67648011973308
150-151	22.77969735342475	27.221934007588604	27.149656591737553	22.84871204724909
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	46.2960716780513
2	42.876777749173755
3	43.57993216339813
4	44.852421417648074
5	44.36415297524556
6	44.863652336555546
7	44.77807051563058
8	43.95179923721595
9	44.203415330023276
10-14	44.84734807762026
15-19	44.93872198595578
20-24	45.14323585528311
25-29	45.196813319071296
30-34	45.39837997448918
35-39	45.4118099248151
40-44	45.42743450212543
45-49	45.484419647435494
50-54	45.43448735950826
55-59	45.48636494126275
60-64	45.520917849719226
65-69	45.508176012510916
70-74	45.47823448169499
75-79	45.49640541135266
80-84	45.49055588761185
85-89	45.470650944521225
90-94	45.47413958868965
95-99	45.42074960155023
100-104	45.45302172180075
105-109	45.50978083716621
110-114	45.50124432299468
115-119	45.44837872851052
120-124	45.48670210875352
125-129	45.470170759572795
130-134	45.484660661489
135-139	45.470548929138495
140-144	45.46923719547262
145-149	45.47938890716275
150-151	45.62840940067384
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	7.5
2	9.5
3	19.5
4	35.5
5	59.5
6	81.5
7	104.5
8	126.0
9	144.0
10	148.5
11	170.5
12	201.5
13	202.5
14	299.0
15	1654.5
16	4092.0
17	6584.0
18	9940.0
19	10454.5
20	10022.5
21	14006.5
22	22535.5
23	32310.0
24	43161.0
25	54503.0
26	72142.5
27	100731.0
28	130139.5
29	154612.5
30	185385.5
31	244603.0
32	310175.0
33	400574.0
34	547993.5
35	717778.0
36	921464.0
37	1173675.5
38	1442837.5
39	1693931.0
40	2039739.0
41	2482714.0
42	2884299.0
43	3317271.0
44	3730939.0
45	4062546.5
46	4268900.5
47	4272682.0
48	4105756.0
49	3791389.0
50	3393746.0
51	2938184.5
52	2455570.5
53	1999307.0
54	1536946.0
55	1111269.5
56	796961.5
57	565878.5
58	394789.5
59	282340.0
60	176487.0
61	87262.5
62	53422.0
63	32137.5
64	14705.0
65	5524.5
66	2253.0
67	1118.0
68	759.0
69	504.0
70	326.5
71	248.5
72	182.5
73	126.0
74	102.0
75	79.5
76	60.5
77	59.5
78	50.0
79	39.5
80	32.5
81	27.0
82	19.0
83	14.5
84	21.0
85	19.0
86	12.5
87	15.5
88	18.5
89	15.5
90	10.0
91	7.5
92	11.5
93	13.5
94	6.5
95	5.0
96	7.0
97	3.5
98	1.5
99	2.5
100	10.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0160385349781992
2	0.00692054072311074
3	0.0056010536138400525
4	0.002261736237301163
5	0.007165829993423879
6	0.008984353894021298
7	0.01080456944475881
8	0.007370519660374844
9	0.008025188264589917
10-14	0.008334760240226499
15-19	0.009332157162823932
20-24	0.011052903685324113
25-29	0.008763086055697446
30-34	0.010056183422782717
35-39	0.006447893673969418
40-44	0.004361412391181645
45-49	0.007172596593984242
50-54	0.011574270258500042
55-59	0.020520392859355294
60-64	0.03602132142303369
65-69	0.05199625035596548
70-74	0.05422584524060493
75-79	0.062485157884583385
80-84	0.04936742603826465
85-89	0.026905018818085324
90-94	0.016621815946502444
95-99	0.010025733720261087
100-104	0.00921137334282146
105-109	0.010397220091024988
110-114	0.008507308554515742
115-119	0.0062344074262899824
120-124	0.005116565013718099
125-129	0.008426109347791394
130-134	0.010894226902183612
135-139	0.007513633262226512
140-144	0.00569003441120882
145-149	0.0035971248578887003
150-151	0.037409151197964036
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	5.9113878E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	95.44695646035193
#Duplication Level	Relative count
1	100.0
2	29.506163268470424
3	22.474610434917434
4	18.6836188851849
5	16.79975191875339
6	16.117528490580664
7	14.722071478409179
8	13.466160167454841
9	13.504922862237382
10++	1305.2329637956432
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE