##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	H0966.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	39674872	
Filtered Sequences	0	
Sequence length	151	
%GC	48	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.070914608117704	34.0	33.0	34.0	31.0	34.0
2	33.27243104905291	34.0	34.0	34.0	31.0	34.0
3	33.33799920009824	34.0	34.0	34.0	31.0	34.0
4	36.599531864904314	37.0	37.0	37.0	35.0	37.0
5	36.616760477513324	37.0	37.0	37.0	35.0	37.0
6	36.601811998284454	37.0	37.0	37.0	36.0	37.0
7	36.54994750833727	37.0	37.0	37.0	35.0	37.0
8	36.601854266851824	37.0	37.0	37.0	36.0	37.0
9	38.57457848887326	39.0	39.0	39.0	39.0	39.0
10-14	38.90475743437811	39.4	39.4	39.4	38.8	39.4
15-19	40.24994590026654	41.0	41.0	41.0	39.0	41.0
20-24	40.19682932814503	41.0	41.0	41.0	39.0	41.0
25-29	40.106228713226855	41.0	40.6	41.0	39.0	41.0
30-34	39.96070578375149	41.0	40.0	41.0	38.6	41.0
35-39	39.73170076515937	41.0	40.0	41.0	37.6	41.0
40-44	39.30318746333952	41.0	39.4	41.0	36.2	41.0
45-49	38.582548021831045	40.0	37.6	41.0	35.0	41.0
50-54	37.82200624617012	39.2	35.8	41.0	35.0	41.0
55-59	36.93180116623943	37.6	35.0	40.6	34.2	41.0
60-64	36.07428825983358	35.6	35.0	39.2	34.0	41.0
65-69	35.30546056456086	35.0	35.0	37.0	33.4	40.2
70-74	34.71694890408216	35.0	35.0	36.0	33.0	38.4
75-79	34.27686969223241	35.0	35.0	35.0	33.0	36.8
80-84	34.04538856231218	35.0	35.0	35.0	33.0	36.0
85-89	33.94644040187451	35.0	35.0	35.0	33.0	36.0
90-94	33.83126051169113	35.0	35.0	35.0	33.0	35.2
95-99	33.73927474800676	35.0	35.0	35.0	33.0	35.0
100-104	33.22998207530449	34.8	33.8	35.0	31.6	35.0
105-109	33.576504307310685	35.0	35.0	35.0	33.0	35.0
110-114	33.53047807690469	35.0	35.0	35.0	32.8	35.0
115-119	33.44149232289899	35.0	35.0	35.0	32.0	35.0
120-124	33.34102909267104	35.0	35.0	35.0	32.0	35.0
125-129	33.318520266429594	35.0	35.0	35.0	32.0	35.0
130-134	33.294812837707454	35.0	35.0	35.0	32.0	35.0
135-139	33.180953425634236	35.0	35.0	35.0	31.4	35.0
140-144	33.04791229068111	35.0	35.0	35.0	31.0	35.0
145-149	32.86371676258968	35.0	34.6	35.0	31.0	35.0
150-151	31.83747761051378	34.5	32.5	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	417.0
1	904.0
2	95954.0
3	28869.0
4	29290.0
5	28192.0
6	27605.0
7	30272.0
8	34328.0
9	36720.0
10	37704.0
11	37488.0
12	35876.0
13	35912.0
14	36748.0
15	38199.0
16	39245.0
17	41279.0
18	42489.0
19	44813.0
20	48065.0
21	53103.0
22	59931.0
23	71055.0
24	81288.0
25	94139.0
26	112486.0
27	136472.0
28	164846.0
29	207136.0
30	264112.0
31	347432.0
32	483640.0
33	736252.0
34	1364252.0
35	4505069.0
36	1.9999592E7
37	9882556.0
38	361142.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.51548456484443	29.562924024867588	20.88207961831031	30.03951179197767
2	26.539977867639692	29.410232740658415	24.795702162011278	19.254087229690615
3	21.915618010171496	27.495011320847173	25.821913972047266	24.76745669693407
4	22.853336189276437	25.58281693501332	26.674794241154398	24.889052634555842
5	24.812396082631878	26.24971182552111	26.724493300664903	22.213398791182108
6	23.56140372590677	26.08803987904323	25.951291363495628	24.399265031554375
7	25.372552371722563	26.19724376721631	26.171862248506116	22.25834161255501
8	22.93654135938758	26.12716975150003	27.297546932159726	23.638741956952657
9	23.361056082450204	25.09470277729864	27.760216583331548	23.784024556919608
10-14	24.006355696967137	26.113451356643335	26.023995974472513	23.85619697191701
15-19	23.983661549619868	25.966966901549405	26.03439360080721	24.01497794802352
20-24	24.1387132350413	25.830651967180025	25.9630720338261	24.067562763952573
25-29	24.162429216429874	25.828038237766854	25.92187244739348	24.087660098409792
30-34	24.215388270371346	25.733846667703595	25.851595194077458	24.199169867847598
35-39	24.28542580846527	25.74860191808736	25.782091738565267	24.183880534882103
40-44	24.325547562166918	25.75425295675096	25.734832277958613	24.185367203123512
45-49	24.323229182326322	25.672334440713517	25.746683581362095	24.257752795598066
50-54	24.326134792345698	25.705564319845735	25.724856384753654	24.243444503054913
55-59	24.35599168956932	25.708385418735475	25.68724268581088	24.248380205884327
60-64	24.315321891145956	25.695718847819265	25.711530089689894	24.277429171344885
65-69	24.342843232242245	25.70182616564326	25.67860883175821	24.276721770356282
70-74	24.36970339971498	25.719557370521244	25.68600936674953	24.224729863014254
75-79	24.342056517192244	25.690428718196014	25.6664910873864	24.30102367722534
80-84	24.343467814443525	25.707342673730505	25.729464977605925	24.219724534220045
85-89	24.361813961486234	25.726615642918375	25.658974110296295	24.252596285299095
90-94	24.357771771505966	25.679194762013623	25.682754278198132	24.280279188282282
95-99	24.30345270123948	25.71174963318813	25.6964452087666	24.288352456805786
100-104	24.341005371210795	25.743405650018303	25.666073056135826	24.24951592263508
105-109	24.30517825903492	25.68768323351766	25.695047852098252	24.312090655349163
110-114	24.337152287382665	25.685499223654762	25.699805202348962	24.27754328661361
115-119	24.369972494840166	25.7363956313943	25.646019979204286	24.247611894561246
120-124	24.292980047389147	25.714128586449696	25.677945685581026	24.31494568058013
125-129	24.336323632991657	25.708102645852783	25.68010979995425	24.27546392120131
130-134	24.359917458677156	25.745638286551454	25.6238085257813	24.27063572899009
135-139	24.3573773559424	25.69518805421241	25.640994035633145	24.306440554212045
140-144	24.372876900487757	25.705491431458828	25.645598292361438	24.276033375691977
145-149	24.375813830718773	25.750332388252062	25.640294157175607	24.233559623853555
150-151	24.38337725847485	25.644040103440513	25.559624003360454	24.412958634724177
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	49.5549963568221
2	45.79406509733031
3	46.68307470710557
4	47.74238882383228
5	47.025794873813986
6	47.96066875746114
7	47.63089398427757
8	46.57528331634024
9	47.145080639369816
10-14	47.86255266888415
15-19	47.99863949764339
20-24	48.206275998993874
25-29	48.25008931483967
30-34	48.41455813821894
35-39	48.46930634334738
40-44	48.51091476529043
45-49	48.58098197792439
50-54	48.56957929540061
55-59	48.604371895453646
60-64	48.59275106249085
65-69	48.61956500259853
70-74	48.59443326272923
75-79	48.643080194417585
80-84	48.56319234866357
85-89	48.614410246785326
90-94	48.638050959788245
95-99	48.59180515804526
100-104	48.590521293845875
105-109	48.617268914384084
110-114	48.61469557399627
115-119	48.617584389401415
120-124	48.607925727969274
125-129	48.61178755419297
130-134	48.630553187667246
135-139	48.66381791015444
140-144	48.648910276179734
145-149	48.60937345457233
150-151	48.79633589319903
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	9.5
2	15.0
3	21.5
4	32.0
5	44.0
6	56.5
7	71.0
8	87.0
9	105.5
10	115.0
11	116.5
12	510.0
13	2066.5
14	3135.0
15	2833.5
16	2855.0
17	3415.0
18	4762.0
19	8347.5
20	15748.5
21	25847.5
22	35490.0
23	53681.5
24	74731.5
25	91880.0
26	117646.0
27	149587.0
28	184460.0
29	205900.5
30	218579.0
31	261811.5
32	307507.5
33	356553.5
34	426952.0
35	500970.5
36	551870.5
37	571342.0
38	613851.0
39	686487.5
40	776270.5
41	876464.0
42	954475.0
43	1026005.0
44	1158232.0
45	1294457.5
46	1426469.0
47	1602469.0
48	1790058.0
49	1972120.0
50	2147886.0
51	2295474.5
52	2366153.5
53	2345375.5
54	2253750.0
55	2055395.0
56	1760979.5
57	1441181.5
58	1157689.5
59	921445.0
60	710085.5
61	544437.5
62	400988.0
63	279652.0
64	195154.0
65	136338.0
66	95454.5
67	70162.5
68	50392.0
69	37151.5
70	25238.0
71	12891.0
72	7116.0
73	3729.0
74	1437.5
75	640.0
76	446.0
77	337.0
78	246.0
79	194.5
80	158.0
81	129.0
82	109.5
83	91.0
84	68.5
85	56.5
86	46.5
87	40.0
88	36.5
89	32.5
90	26.5
91	21.0
92	17.0
93	16.5
94	14.0
95	13.0
96	14.0
97	9.5
98	6.0
99	6.5
100	12.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01564718343640781
2	0.006669208661845211
3	0.005156916448274868
4	0.002215508092880552
5	0.006870847623654589
6	0.008844388962363885
7	0.010775082021688689
8	0.007246400190024558
9	0.007866439997588397
10-14	0.008194607407933163
15-19	0.009183142418203644
20-24	0.010834061418017934
25-29	0.008602422208192632
30-34	0.009797637154317727
35-39	0.006347594517759251
40-44	0.0042757541851678816
45-49	0.007208592884685299
50-54	0.011785293220353679
55-59	0.020888788248642615
60-64	0.03650471764596997
65-69	0.05262474444782078
70-74	0.05505247754800569
75-79	0.06343309689820802
80-84	0.05008258123680903
85-89	0.027169841909004774
90-94	0.016533386673560028
95-99	0.010059263707265394
100-104	0.009150880184314142
105-109	0.010369787708451839
110-114	0.008434557772486324
115-119	0.006203422660065545
120-124	0.005012744590581162
125-129	0.008335754681199728
130-134	0.010761471391766558
135-139	0.0074762686064872505
140-144	0.005581366462883611
145-149	0.0034984359873927256
150-151	0.03726161989886193
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	3.9674872E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	94.2194761751561
#Duplication Level	Relative count
1	100.0
2	40.032186682760006
3	32.53537182324147
4	28.44498088915711
5	27.311741433648496
6	26.547307718098303
7	24.36129551398109
8	23.992489774022665
9	22.23563334003889
10++	1015.6507744920539
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE