##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	H0967.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	59250486	
Filtered Sequences	0	
Sequence length	151	
%GC	47	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.9743251726239	34.0	33.0	34.0	31.0	34.0
2	33.15908483856149	34.0	33.0	34.0	31.0	34.0
3	33.2604698634877	34.0	34.0	34.0	31.0	34.0
4	36.54266292431762	37.0	37.0	37.0	35.0	37.0
5	36.517104990497465	37.0	37.0	37.0	35.0	37.0
6	36.50907285384967	37.0	37.0	37.0	35.0	37.0
7	36.52020408743989	37.0	37.0	37.0	35.0	37.0
8	36.43580727759769	37.0	37.0	37.0	35.0	37.0
9	38.42101060571891	39.0	39.0	39.0	38.0	39.0
10-14	38.76112365390556	39.4	39.4	39.4	38.2	39.4
15-19	40.06620117175073	41.0	40.0	41.0	39.0	41.0
20-24	40.01423253135848	41.0	40.0	41.0	39.0	41.0
25-29	39.91893209112242	41.0	40.0	41.0	38.2	41.0
30-34	39.767972225578035	41.0	40.0	41.0	38.0	41.0
35-39	39.54680772745054	41.0	40.0	41.0	37.4	41.0
40-44	39.15234170399885	41.0	39.4	41.0	35.8	41.0
45-49	38.491997797283894	40.0	37.6	41.0	35.0	41.0
50-54	37.803083265848656	39.4	36.2	41.0	34.8	41.0
55-59	36.960537711032444	38.0	35.0	40.6	34.0	41.0
60-64	36.11315084234077	36.4	35.0	39.4	33.6	41.0
65-69	35.32646107240369	35.0	35.0	37.6	33.0	40.6
70-74	34.698635788405184	35.0	35.0	36.2	33.0	39.0
75-79	34.21377973507255	35.0	35.0	35.4	33.0	37.2
80-84	33.93642603370375	35.0	35.0	35.0	33.0	36.2
85-89	33.79808841399208	35.0	35.0	35.0	33.0	36.0
90-94	33.65801391401245	35.0	35.0	35.0	33.0	35.8
95-99	33.549119232540974	35.0	35.0	35.0	32.2	35.0
100-104	33.028706539217254	34.8	33.8	35.0	30.8	35.0
105-109	33.37854687976737	35.0	35.0	35.0	32.0	35.0
110-114	33.3326353913789	35.0	35.0	35.0	32.0	35.0
115-119	33.24245561800118	35.0	35.0	35.0	31.0	35.0
120-124	33.14303514067378	35.0	35.0	35.0	31.0	35.0
125-129	33.117969753024475	35.0	35.0	35.0	31.0	35.0
130-134	33.0917046385071	35.0	35.0	35.0	31.0	35.0
135-139	32.9742813181313	35.0	34.8	35.0	31.0	35.0
140-144	32.834173120537784	35.0	34.0	35.0	30.8	35.0
145-149	32.641305794521244	35.0	34.0	35.0	30.0	35.0
150-151	31.624654893126106	34.5	32.5	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	723.0
1	1435.0
2	181168.0
3	54696.0
4	53235.0
5	54186.0
6	55289.0
7	60822.0
8	67669.0
9	70143.0
10	68836.0
11	68468.0
12	66448.0
13	63683.0
14	63099.0
15	61365.0
16	64749.0
17	67304.0
18	70075.0
19	75730.0
20	79315.0
21	86220.0
22	98400.0
23	117262.0
24	139325.0
25	160803.0
26	192829.0
27	234856.0
28	284860.0
29	353444.0
30	451000.0
31	598236.0
32	840008.0
33	1262619.0
34	2257327.0
35	6619878.0
36	2.7432162E7
37	1.5875415E7
38	897404.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.169217831188305	30.382308377385094	21.312842541945667	29.13563124948093
2	25.940612916784865	29.844558931332337	25.413221979211727	18.80160617267107
3	21.370632833665994	28.171529451617744	26.253327062420034	24.204510652296225
4	22.288890148731	26.177857341739184	27.422695929607105	24.11055657992271
5	24.339452057920557	26.880813587847683	27.06141614414015	21.71831821009161
6	23.04533568649321	26.880584000448305	26.543660275371018	23.530420037687467
7	24.856131163311257	26.814879201321546	26.771541528369415	21.557448106997786
8	22.53045110596602	26.63601926093933	27.882976783426372	22.950552849668277
9	22.72910193281244	25.669683871208406	28.52589525770523	23.075318938273924
10-14	23.394941427688178	26.756438667059832	26.693847995723562	23.154771909528428
15-19	23.351308183626063	26.620530603558855	26.68224690549471	23.345914307320367
20-24	23.457329066588663	26.503078555860814	26.598855376686597	23.440737000863923
25-29	23.51846130192219	26.485374107353348	26.53186015223113	23.46430443849333
30-34	23.607202161233875	26.378780341561875	26.43824203604587	23.575775461158376
35-39	23.65543497148741	26.401568401407665	26.37767811340403	23.565318513700895
40-44	23.6713889587667	26.401026137872698	26.38678495003638	23.540799953324225
45-49	23.698062311717884	26.324548952596473	26.36384130527794	23.613547430407706
50-54	23.65619329922544	26.38430780562866	26.3407726161805	23.6187262789654
55-59	23.681938666271744	26.373319486032216	26.33495119449262	23.609790653203415
60-64	23.68832471530068	26.338597597062908	26.331933370839305	23.641144316797106
65-69	23.707364581750586	26.379121146087286	26.334642308610768	23.578871963551364
70-74	23.721370482127295	26.36285616298296	26.305450181813132	23.610323173076612
75-79	23.683187012945176	26.354929827384506	26.321607588967037	23.640275570703285
80-84	23.666004733075926	26.401861147672722	26.325388492338302	23.606745626913046
85-89	23.70436088407181	26.382956414726564	26.335245829796705	23.577436871404913
90-94	23.65595907248756	26.378415967244447	26.335204091281984	23.630420868986015
95-99	23.68522540176345	26.366229331562774	26.348704667121766	23.599840599552017
100-104	23.701353447505625	26.424836512322614	26.284702601852306	23.58910743831946
105-109	23.686978111335137	26.336116930844184	26.33571655488914	23.641188402931544
110-114	23.650433417030296	26.392420822651786	26.337591661000975	23.619554099316943
115-119	23.709557005534823	26.39325333737897	26.305514872788965	23.591674784297247
120-124	23.706818151728665	26.354004041530736	26.318113215615664	23.621064591124934
125-129	23.63858916382776	26.328570842824163	26.393055800928384	23.639784192419697
130-134	23.71998604253284	26.373361827837016	26.317108692624313	23.58954343700583
135-139	23.687914523458407	26.374574976457378	26.294373380781817	23.643137119302395
140-144	23.71996868464723	26.357802203642517	26.300631477940968	23.621597633769287
145-149	23.75217008643781	26.34587318131924	26.302788761078222	23.599167971164732
150-151	23.747622787211853	26.272347328189177	26.232342939086035	23.74768694551294
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	48.304849080669236
2	44.74221908945594
3	45.57514348596222
4	46.39944672865371
5	46.05777026801216
6	46.575755724180674
7	46.41357927030904
8	45.4810039556343
9	45.804420871086364
10-14	46.5497133372166
15-19	46.69722249094643
20-24	46.89806606745259
25-29	46.98276574041552
30-34	47.182977622392244
35-39	47.220753485188304
40-44	47.21218891209092
45-49	47.31160974212559
50-54	47.27491957819085
55-59	47.29172931947516
60-64	47.32946903209779
65-69	47.28623654530195
70-74	47.33169365520391
75-79	47.323462583648464
80-84	47.272750359988976
85-89	47.281797755476724
90-94	47.28637994147357
95-99	47.285066001315464
100-104	47.290460885825084
105-109	47.32816651426668
110-114	47.26998751634724
115-119	47.301231789832066
120-124	47.3278827428536
125-129	47.27837335624746
130-134	47.30952947953867
135-139	47.331051642760805
140-144	47.34156631841652
145-149	47.35133805760254
150-151	47.49530973272479
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8.0
1	9.5
2	13.5
3	20.5
4	39.0
5	61.0
6	92.0
7	117.0
8	131.0
9	158.5
10	177.0
11	179.0
12	192.5
13	226.0
14	274.5
15	593.5
16	2776.5
17	4676.0
18	3700.0
19	4344.0
20	9262.0
21	39489.0
22	71680.5
23	91112.5
24	113735.5
25	140614.0
26	184248.0
27	223801.0
28	260344.5
29	334417.0
30	437801.5
31	541929.5
32	660206.0
33	721712.0
34	764990.5
35	876197.0
36	1011302.0
37	1117869.0
38	1172966.5
39	1275820.5
40	1439343.0
41	1600487.0
42	1770022.0
43	1979911.5
44	2185084.5
45	2347765.0
46	2498246.5
47	2699505.0
48	2945571.5
49	3073036.0
50	3022427.5
51	2940968.0
52	2926698.0
53	2871256.0
54	2702684.5
55	2466262.0
56	2170983.0
57	1818212.0
58	1477739.0
59	1186827.5
60	940724.5
61	710306.0
62	496532.0
63	343222.5
64	226816.0
65	136539.5
66	81616.5
67	48878.0
68	29282.5
69	18328.0
70	10784.0
71	5721.5
72	3454.0
73	2190.5
74	1377.0
75	983.0
76	810.0
77	691.0
78	456.0
79	307.5
80	250.5
81	169.0
82	126.5
83	100.0
84	90.0
85	73.5
86	59.0
87	49.5
88	42.0
89	34.0
90	24.5
91	23.5
92	16.5
93	11.0
94	13.0
95	10.5
96	10.0
97	12.5
98	9.5
99	6.0
100	12.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016145015249326395
2	0.007262387687419138
3	0.00574003730534801
4	0.002428672061862919
5	0.007375466928659454
6	0.00898895580367054
7	0.010833666410770031
8	0.007361964929705387
9	0.007925673386037712
10-14	0.008296978357274573
15-19	0.009322117627862158
20-24	0.010981513299317073
25-29	0.008740856572889545
30-34	0.010056288820989587
35-39	0.006441128601037973
40-44	0.004420554457561749
45-49	0.007344749881038951
50-54	0.011674165845660743
55-59	0.020231057682801118
60-64	0.03511194828005293
65-69	0.050570724432538836
70-74	0.05269357621809212
75-79	0.060719501946363774
80-84	0.04815656701955153
85-89	0.02672011838012603
90-94	0.0165858555151767
95-99	0.010101182967511863
100-104	0.009181021738792152
105-109	0.010411728943455417
110-114	0.008448875845507834
115-119	0.006283830313223084
120-124	0.005119958003382453
125-129	0.008508959740853434
130-134	0.010969023950284559
135-139	0.007649895057400879
140-144	0.005705944757988989
145-149	0.003636763418278122
150-151	0.03710518087564716
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	5.9250486E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	95.00450090185356
#Duplication Level	Relative count
1	100.0
2	31.310289389067524
3	24.062165058949624
4	20.793140407288316
5	20.424705251875668
6	17.79876741693462
7	17.329849946409432
8	16.613076098606644
9	15.032154340836012
10++	1076.400053590568
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE