FastQC Report![[OK]](Icons/tick.png)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | M4533.8125.1.90603.CGTACTA-ACTGCAT.anqdp.trim2.fastq.gz |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 1521548 |
| Filtered Sequences | 0 |
| Sequence length | 15-151 |
| %GC | 47 |
Per base sequence quality
Per sequence quality scores
![[FAIL]](Icons/error.png)
Per base sequence content
Per base GC content
![[WARN]](Icons/warning.png)
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| GTGTTAGACCGTGTGGTTAAACAGGTCGATATAGCACAAAAACGTATCCT | 2828 | 0.18586334443606117 | No Hit |
| GTATAGCCACGGTCTGTAAGAACTTAACGAACCCTTCTACCGCTTTAACG | 1888 | 0.12408415639861509 | No Hit |
| GTCTAACACAAAGGGAATTAACTCATCGTTTTTTCCCTGACGTACACGCA | 1747 | 0.11481727819299817 | No Hit |
| GTCTAAGACTGCTTAATTTAGCGTGCAAACGTTAGCAGACAATAAAATTT | 1731 | 0.1137657175455523 | No Hit |
| GTTATAAGGAGGGGCTATGTTCGACGACGGATTAATAGCACAACCTAGCA | 1701 | 0.11179404133159125 | No Hit |
| TAATAAGATATGCCGTGTAGTCGGAACCTACACCGGTGCCGTCATCTGCC | 1573 | 0.10338155615202413 | No Hit |
| TCACTATACAATGCTGACCAATAAGGACTCGATTGATGTGGTGCTAAGTT | 1564 | 0.10279005328783582 | No Hit |
| GTGTTGGTGTTGAGCGTGTGTTCCAAACACATAGCCCAATTGTTGCGAGC | 1539 | 0.10114698977620161 | No Hit |
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| CACCA | 434185 | 2.1625514 | 7.287961 | 7 |
| CAACA | 470810 | 2.0992777 | 5.641471 | 4 |
| TGTTG | 468980 | 2.0475483 | 6.2803683 | 2 |
| TTGGT | 448040 | 1.956125 | 5.0535355 | 4 |
| CAGCG | 329840 | 1.8063027 | 5.836422 | 9 |
| GCCTT | 332270 | 1.6361827 | 5.9471025 | 1 |
| AAGGC | 326460 | 1.6004783 | 5.2213464 | 5 |
| CTGTT | 347800 | 1.5305423 | 6.419953 | 9 |
| ACCGC | 271555 | 1.4989287 | 6.3971214 | 8 |
| GCACC | 269635 | 1.4883307 | 5.6336455 | 6 |
| AACAG | 335645 | 1.4848017 | 5.5132847 | 7 |
| CTGCT | 298795 | 1.4713433 | 6.14068 | 9 |
| GTGCT | 294135 | 1.4369824 | 7.184135 | 1 |
| GGTTA | 326750 | 1.4290681 | 5.2502065 | 1 |
| AGCAC | 287685 | 1.4215852 | 6.672952 | 5 |
| CACCG | 251380 | 1.3875667 | 5.1835966 | 7 |
| TAGCA | 311345 | 1.3749049 | 5.5165358 | 4 |
| AACAC | 306045 | 1.3646129 | 6.5568304 | 5 |
| GTGTT | 310075 | 1.3537753 | 9.12179 | 1 |
| ACACC | 270155 | 1.3455648 | 6.5192804 | 6 |
| ACCAT | 300935 | 1.3394897 | 5.4145393 | 8 |
| TATTG | 333875 | 1.3153225 | 5.560893 | 2 |
| TGCTA | 292460 | 1.2892576 | 5.8823934 | 2 |
| GTTCA | 272370 | 1.2006946 | 5.2886987 | 1 |
| TGAAC | 270200 | 1.1932079 | 5.1114755 | 5 |
| ACTGC | 241040 | 1.189015 | 6.3345413 | 8 |
| TACCG | 239745 | 1.182627 | 5.7097054 | 7 |
| TGTTA | 289480 | 1.1404253 | 6.504437 | 2 |
| TAACA | 282865 | 1.1271417 | 5.3693795 | 4 |
| ATACC | 246835 | 1.0986857 | 6.295902 | 6 |
| GTTAG | 244850 | 1.0708717 | 5.422136 | 3 |
| CCTTA | 238120 | 1.0580473 | 5.1786776 | 2 |
| TAAGG | 240330 | 1.0529381 | 5.32954 | 5 |
| ACCGT | 210605 | 1.0388836 | 7.724262 | 8 |
| GTATT | 251850 | 0.99217963 | 6.435608 | 1 |
| TACTG | 207860 | 0.9163137 | 5.102567 | 2 |
| CCGTG | 164310 | 0.89824265 | 6.4152427 | 9 |
| GTACA | 174035 | 0.7685415 | 5.2047033 | 1 |
| TTAGA | 190925 | 0.7534747 | 5.4298277 | 4 |
| GACTG | 151245 | 0.7401902 | 5.2300606 | 7 |
| TCTAA | 177850 | 0.7074498 | 5.270253 | 2 |
| GACCG | 121810 | 0.66706806 | 5.0072036 | 7 |
| GTCTA | 118755 | 0.5235102 | 5.651334 | 1 |
| TAGAC | 114715 | 0.5065834 | 5.3847303 | 5 |
| GTATA | 97670 | 0.3854491 | 5.4413195 | 1 |