FastQC Report![[OK]](Icons/tick.png)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | M4533.8125.1.90603.CGTACTA-ACTGCAT.anqdp.fastq.gz |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 1521692 |
| Filtered Sequences | 0 |
| Sequence length | 151 |
| %GC | 47 |
Per base sequence quality
Per sequence quality scores
![[FAIL]](Icons/error.png)
Per base sequence content
Per base GC content
![[WARN]](Icons/warning.png)
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| GTGTTAGACCGTGTGGTTAAACAGGTCGATATAGCACAAAAACGTATCCT | 2828 | 0.18584575590855443 | No Hit |
| GTATAGCCACGGTCTGTAAGAACTTAACGAACCCTTCTACCGCTTTAACG | 1888 | 0.12407241412848329 | No Hit |
| GTCTAACACAAAGGGAATTAACTCATCGTTTTTTCCCTGACGTACACGCA | 1747 | 0.11480641286147261 | No Hit |
| GTCTAAGACTGCTTAATTTAGCGTGCAAACGTTAGCAGACAATAAAATTT | 1731 | 0.11375495172479055 | No Hit |
| GTTATAAGGAGGGGCTATGTTCGACGACGGATTAATAGCACAACCTAGCA | 1701 | 0.1117834620935117 | No Hit |
| TAATAAGATATGCCGTGTAGTCGGAACCTACACCGGTGCCGTCATCTGCC | 1573 | 0.10337177300005519 | No Hit |
| TCACTATACAATGCTGACCAATAAGGACTCGATTGATGTGGTGCTAAGTT | 1564 | 0.10278032611067153 | No Hit |
| GTGTTGGTGTTGAGCGTGTGTTCCAAACACATAGCCCAATTGTTGCGAGC | 1539 | 0.10113741808460582 | No Hit |
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| CACCA | 434345 | 2.1335351 | 7.292958 | 7 |
| CAACA | 472345 | 2.0871847 | 5.5634766 | 4 |
| TGTTG | 471210 | 2.0350742 | 6.231225 | 2 |
| CAGCG | 330065 | 1.7963277 | 5.9721847 | 9 |
| GTTAA | 424560 | 1.6630057 | 5.006585 | 3 |
| GCCTT | 335660 | 1.6247298 | 5.806161 | 1 |
| AAGGC | 328955 | 1.610494 | 5.4048038 | 5 |
| CTGTT | 347840 | 1.5047575 | 6.3528676 | 9 |
| ACCGC | 273110 | 1.488829 | 6.6071663 | 8 |
| GCACC | 272720 | 1.486703 | 5.685609 | 6 |
| AACAG | 336705 | 1.485354 | 5.401853 | 7 |
| CTGCT | 302415 | 1.4638106 | 6.1014495 | 9 |
| GTGCT | 295985 | 1.4303097 | 6.8300986 | 1 |
| AGCAC | 290655 | 1.4253504 | 6.754618 | 5 |
| GGTTA | 327395 | 1.4232101 | 5.4093027 | 1 |
| CACCG | 252835 | 1.3783022 | 5.0765796 | 7 |
| TAGCA | 313560 | 1.3742632 | 5.524599 | 4 |
| AACAC | 307480 | 1.3586838 | 6.518329 | 5 |
| GTGTT | 313560 | 1.3542112 | 9.25002 | 1 |
| ACACC | 274555 | 1.3486347 | 6.282053 | 6 |
| GCTTA | 306795 | 1.3358767 | 5.1174536 | 1 |
| ACCAT | 303925 | 1.3342489 | 5.4886065 | 8 |
| TATTG | 332930 | 1.2956165 | 5.4231205 | 2 |
| TGCTA | 293990 | 1.28012 | 5.7575355 | 2 |
| TGAAC | 273010 | 1.1965415 | 5.1219306 | 5 |
| GTTCA | 271945 | 1.1841294 | 5.424692 | 1 |
| ACTGC | 242940 | 1.1836183 | 6.281012 | 8 |
| TACCG | 241635 | 1.1772604 | 5.854877 | 7 |
| TGTTA | 290045 | 1.128727 | 6.5729585 | 2 |
| TAACA | 283120 | 1.1180922 | 5.184129 | 4 |
| ATACC | 251655 | 1.1047806 | 6.3372273 | 6 |
| GTTAG | 244875 | 1.0644895 | 5.5786424 | 3 |
| TAAGG | 242590 | 1.0614536 | 5.3610644 | 5 |
| CCTTA | 239760 | 1.0457215 | 5.084285 | 2 |
| ACCGT | 210650 | 1.0262995 | 7.5415115 | 8 |
| GTATT | 252205 | 0.9814704 | 6.289789 | 1 |
| AATAC | 236555 | 0.9341984 | 5.0041647 | 5 |
| TACTG | 212115 | 0.9236118 | 5.043844 | 7 |
| CCGTG | 165280 | 0.89366597 | 6.211569 | 9 |
| GTACA | 174235 | 0.7636329 | 5.2733335 | 1 |
| GACTG | 154990 | 0.75386786 | 5.0229077 | 7 |
| TTAGA | 191440 | 0.7498724 | 5.4326777 | 4 |
| TCTAA | 178020 | 0.698465 | 5.42152 | 2 |
| GTCTA | 118845 | 0.5174865 | 5.840746 | 1 |
| TAGAC | 115270 | 0.5052026 | 5.2668915 | 5 |
| GTATA | 97075 | 0.38024375 | 5.366461 | 1 |