##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	M5953_TCTTGCTT-AAGTACCT_L001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2159084	
Filtered Sequences	0	
Sequence length	151	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35382921646402	33.0	32.0	34.0	32.0	34.0
2	32.518839933972	33.0	32.0	34.0	32.0	34.0
3	32.702282542040976	33.0	32.0	34.0	32.0	34.0
4	32.834076395360256	33.0	33.0	34.0	32.0	34.0
5	32.71078244292487	33.0	32.0	34.0	32.0	34.0
6	35.11920240250032	37.0	34.0	37.0	33.0	37.0
7	35.20046047305246	37.0	34.0	37.0	33.0	37.0
8	35.23107021310889	37.0	34.0	37.0	33.0	37.0
9	35.1950725400216	37.0	34.0	37.0	33.0	37.0
10-14	36.000483260493795	37.4	35.6	37.4	34.6	37.4
15-19	37.08081019543473	38.0	38.0	38.0	37.0	38.0
20-24	37.35315476377944	38.4	38.0	38.4	37.0	38.4
25-29	37.73764133308384	39.0	38.0	39.0	37.0	39.0
30-34	37.70499193176366	39.0	38.0	39.0	37.0	39.0
35-39	37.679945569510025	39.0	38.0	39.0	37.0	39.0
40-44	37.64560387645872	39.0	38.0	39.0	36.8	39.0
45-49	37.58369373308311	39.0	38.0	39.0	36.6	39.0
50-54	37.574236481767265	39.0	38.0	39.0	36.4	39.0
55-59	37.61090314225847	39.0	38.0	39.0	36.8	39.0
60-64	37.57259069123758	39.0	38.0	39.0	36.8	39.0
65-69	37.512320409951634	39.0	38.0	39.0	36.4	39.0
70-74	37.49075663568439	39.0	38.0	39.0	36.4	39.0
75-79	37.49765502407502	39.0	38.0	39.0	36.4	39.0
80-84	37.5176996355862	39.0	38.0	39.0	36.6	39.0
85-89	37.50347100900196	39.0	38.0	39.0	36.6	39.0
90-94	37.48389150213702	39.0	38.0	39.0	36.6	39.0
95-99	37.44791374490293	39.0	38.0	39.0	36.4	39.0
100-104	37.39388536990687	39.0	38.0	39.0	36.4	39.0
105-109	37.349764437140934	39.0	38.0	39.0	36.0	39.0
110-114	37.238176745323486	39.0	38.0	39.0	36.0	39.0
115-119	37.234137995557376	39.0	38.0	39.0	36.0	39.0
120-124	37.27479940567388	39.0	38.0	39.0	36.0	39.0
125-129	37.20805600893712	39.0	38.0	39.0	36.0	39.0
130-134	37.12369634530199	39.0	38.0	39.0	35.4	39.0
135-139	37.03840739869315	39.0	38.0	39.0	35.0	39.0
140-144	36.854540258739355	39.0	38.0	39.0	34.6	39.0
145-149	36.70375751939248	39.0	38.0	39.0	34.2	39.0
150-151	35.89695537552036	38.5	37.5	39.0	32.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	144.0
3	148.0
4	244.0
5	298.0
6	283.0
7	338.0
8	382.0
9	413.0
10	492.0
11	495.0
12	540.0
13	626.0
14	810.0
15	887.0
16	1044.0
17	1310.0
18	1785.0
19	2148.0
20	2903.0
21	3772.0
22	4639.0
23	5605.0
24	6780.0
25	8226.0
26	9683.0
27	11205.0
28	13170.0
29	15124.0
30	18047.0
31	21867.0
32	26668.0
33	33749.0
34	44315.0
35	64136.0
36	111896.0
37	329897.0
38	1415015.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.910280470792245	31.77310377919525	22.758308616061257	27.55830713395125
2	24.130279322156987	31.548980956739058	25.7636108646074	18.55712885649655
3	20.51587617711956	29.299554811206974	26.983526347284307	23.201042664389156
4	21.72124845536348	26.493411094704978	28.511905974941225	23.27343447499032
5	23.664155725298322	27.47605002862325	28.176949113605588	20.682845132472846
6	22.149578247071446	27.965007382760465	27.087459311448743	22.797955058719346
7	24.036026388968658	27.596749362229538	27.64051792334157	20.726706325460242
8	21.894747957930306	27.572294547132024	28.529042871884556	22.00391462305311
9	21.877518429111607	26.513326947909395	29.32966017070202	22.279494452276985
10-14	22.546589201717023	27.650327638943185	27.507461497561003	22.295621661778792
15-19	22.41375509243735	27.514232887650504	27.56265156890607	22.509360451006074
20-24	22.575129082518327	27.41302330062193	27.458866815742233	22.55298080111751
25-29	22.62377934346232	27.399526836380613	27.435477267211468	22.5412165529456
30-34	22.691131980043387	27.28859090243826	27.34551318985273	22.67476392766562
35-39	22.715429320952772	27.308793914456313	27.295214081527163	22.680562683063744
40-44	22.758521669374606	27.30081830998701	27.263682191151435	22.67697782948695
45-49	22.769683810356614	27.24599876614342	27.261894395956805	22.72242302754316
50-54	22.730935896889605	27.291184594948596	27.273704960066397	22.704174548095395
55-59	22.77799288957725	27.2504821489113	27.27662286414053	22.69490209737092
60-64	22.76882233391568	27.21920036459906	27.264376930216706	22.747600371268557
65-69	22.75454776192126	27.26187586958173	27.270879687867634	22.712696680629378
70-74	22.767655172286023	27.256595852685678	27.291490280137314	22.684258694890982
75-79	22.747943109207423	27.245118763327408	27.25625311474681	22.750685012718357
80-84	22.75890146006362	27.248379405340412	27.257568487377053	22.735150647218912
85-89	22.767127170596417	27.306691170885433	27.229677029703335	22.69650462881481
90-94	22.720811232911732	27.287646057309487	27.233206304154912	22.758336405623865
95-99	22.720023861971093	27.31638046504907	27.255984482308236	22.7076111906716
100-104	22.727888308189957	27.295593872216177	27.26477524728079	22.711742572313074
105-109	22.755557449362783	27.21394813726562	27.25697564337469	22.773518769996905
110-114	22.752593229350964	27.24442402426214	27.254076265675632	22.748906480711266
115-119	22.7489898493991	27.32870050447319	27.211289602442516	22.711020043685192
120-124	22.752871124977073	27.27290832593822	27.238301057300223	22.73591949178448
125-129	22.72989841988547	27.286052789053134	27.211697182694145	22.772351608367252
130-134	22.73534517415719	27.297168614097462	27.245980239768347	22.721505971977006
135-139	22.72355313642267	27.298446933977555	27.214846666456697	22.763153263143074
140-144	22.78796934255453	27.319585527936844	27.203221366097846	22.68922376341078
145-149	22.83580444299527	27.303745477248686	27.182230983139142	22.678219096616896
150-151	22.845035209375826	27.254937742116564	27.097440396019795	22.802586652487815
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	45.46858760474349
2	42.68740817865354
3	43.716918841508715
4	44.9946829303538
5	44.34700085777117
6	44.947533305790785
7	44.7627327144289
8	43.89866258098342
9	44.15701288138859
10-14	44.842210863495815
15-19	44.92311554344342
20-24	45.12810988363584
25-29	45.16499589640792
30-34	45.36589590770901
35-39	45.39599200401652
40-44	45.43549949886155
45-49	45.492106837899776
50-54	45.43511044498501
55-59	45.47289498694817
60-64	45.51642270518423
65-69	45.46724444255064
70-74	45.451913867177005
75-79	45.498628121925776
80-84	45.494052107282535
85-89	45.46363179941123
90-94	45.4791476385356
95-99	45.42763505264269
100-104	45.43963088050303
105-109	45.529076219359695
110-114	45.501499710062234
115-119	45.46000989308429
120-124	45.488790616761555
125-129	45.502250028252725
130-134	45.45685114613419
135-139	45.48670639956574
140-144	45.477193105965306
145-149	45.51402353961217
150-151	45.64762186186364
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.5
4	1.5
5	3.5
6	3.5
7	1.5
8	2.5
9	3.5
10	6.0
11	8.0
12	6.5
13	6.5
14	10.0
15	61.0
16	157.0
17	263.5
18	394.0
19	411.0
20	388.5
21	530.0
22	864.0
23	1241.5
24	1637.0
25	2076.5
26	2684.5
27	3762.5
28	4869.0
29	5709.0
30	6882.0
31	8958.0
32	11477.0
33	14910.5
34	20061.5
35	26400.5
36	33959.0
37	42973.5
38	52797.5
39	61889.0
40	74488.0
41	90267.5
42	105099.0
43	121273.5
44	136125.5
45	148046.5
46	155324.5
47	155412.0
48	149531.5
49	138035.0
50	123308.0
51	107156.0
52	90008.5
53	73384.5
54	56591.5
55	40972.5
56	29202.0
57	20835.5
58	14659.0
59	10281.5
60	6332.5
61	3169.5
62	1965.5
63	1191.0
64	545.0
65	197.5
66	77.0
67	39.0
68	23.5
69	17.0
70	11.0
71	6.0
72	3.5
73	3.0
74	3.0
75	4.0
76	3.5
77	2.5
78	1.5
79	3.5
80	4.0
81	3.0
82	3.0
83	1.5
84	1.5
85	1.5
86	2.0
87	1.5
88	1.5
89	1.5
90	0.5
91	0.5
92	1.0
93	1.5
94	2.0
95	3.0
96	3.0
97	3.0
98	4.0
99	5.5
100	14.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	2159084.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	80.31859014214352
#Duplication Level	Relative count
1	100.0
2	45.931372549019606
3	34.689542483660134
4	28.070079883805374
5	23.493100944081338
6	19.526143790849673
7	16.73202614379085
8	14.079520697167755
9	11.65032679738562
10++	68.9360929557008
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE