##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	N0057.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	82749932	
Filtered Sequences	0	
Sequence length	150	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.426445438045796	32.0	32.0	32.0	27.0	32.0
2	31.087574428459952	32.0	32.0	32.0	32.0	32.0
3	30.8317343269841	32.0	32.0	32.0	27.0	32.0
4	30.82198223437815	32.0	32.0	32.0	27.0	32.0
5	30.714564043387977	32.0	32.0	32.0	27.0	32.0
6	35.07434847197216	37.0	37.0	37.0	32.0	37.0
7	35.08510044455384	37.0	37.0	37.0	32.0	37.0
8	35.11656422871743	37.0	37.0	37.0	32.0	37.0
9	35.11056447756356	37.0	37.0	37.0	32.0	37.0
10-14	35.0262281593174	37.0	37.0	37.0	32.0	37.0
15-19	34.95384323457813	37.0	37.0	37.0	32.0	37.0
20-24	34.91159933279462	37.0	37.0	37.0	32.0	37.0
25-29	34.8973371047604	37.0	37.0	37.0	32.0	37.0
30-34	34.8545852098102	37.0	37.0	37.0	32.0	37.0
35-39	34.79174302886436	37.0	37.0	37.0	30.0	37.0
40-44	34.725567977506	37.0	37.0	37.0	27.0	37.0
45-49	34.65247835369822	37.0	37.0	37.0	27.0	37.0
50-54	34.5882824701294	37.0	37.0	37.0	27.0	37.0
55-59	34.52036380162826	37.0	37.0	37.0	27.0	37.0
60-64	34.448313936983055	37.0	37.0	37.0	27.0	37.0
65-69	34.365870098841896	37.0	37.0	37.0	27.0	37.0
70-74	34.281086672071226	37.0	37.0	37.0	27.0	37.0
75-79	34.215156510340094	37.0	37.0	37.0	27.0	37.0
80-84	34.12089376943536	37.0	37.0	37.0	27.0	37.0
85-89	34.00947415884281	37.0	37.0	37.0	27.0	37.0
90-94	33.871779285570895	37.0	37.0	37.0	27.0	37.0
95-99	33.72855731168456	37.0	36.0	37.0	25.0	37.0
100-104	33.5816240960778	37.0	32.0	37.0	22.0	37.0
105-109	33.424308602452996	37.0	32.0	37.0	22.0	37.0
110-114	33.28181156692673	37.0	32.0	37.0	22.0	37.0
115-119	33.13386988160909	37.0	32.0	37.0	22.0	37.0
120-124	33.02202393833991	37.0	32.0	37.0	22.0	37.0
125-129	32.89053159826162	37.0	32.0	37.0	22.0	37.0
130-134	32.787383257305876	37.0	32.0	37.0	22.0	37.0
135-139	32.694915572861134	37.0	32.0	37.0	22.0	37.0
140-144	32.55729571113122	37.0	32.0	37.0	22.0	37.0
145-149	32.295610635667956	37.0	32.0	37.0	16.6	37.0
150	30.483619696509237	37.0	27.0	37.0	13.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	94.0
5	1283.0
6	2104.0
7	1801.0
8	1560.0
9	1577.0
10	1565.0
11	3636.0
12	10092.0
13	16912.0
14	23301.0
15	30014.0
16	37194.0
17	44333.0
18	56443.0
19	84234.0
20	137391.0
21	217430.0
22	321933.0
23	451732.0
24	607485.0
25	789168.0
26	996001.0
27	1245027.0
28	1550619.0
29	1955055.0
30	2518366.0
31	3384825.0
32	4860281.0
33	7821584.0
34	1.4761324E7
35	2.8557967E7
36	1.2257601E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.457526723440818	32.43061648576095	23.287261450611332	26.824595340186896
2	23.382202209529105	31.461189833371005	26.32390897129053	18.832698985809365
3	21.232231128916034	30.263809436657592	26.347733528747103	22.156225905679268
4	21.23810323219102	28.202241565408993	27.65183598310612	22.907819219293867
5	22.99546465861752	28.596546118659163	27.407011626125733	21.000977596597583
6	22.45985374823675	28.7155995228755	26.820640523790185	22.00390620509756
7	23.508286178741287	28.421296200450968	27.359410403749223	20.71100721705852
8	21.53653613938536	28.39574376104594	28.571260588456195	21.49645951111251
9	21.41493788535127	27.556098151490975	29.273114900858054	21.755849062299696
10-14	22.202800258709004	28.449539591521074	27.586019303246434	21.761640846523488
15-19	22.15401932976176	28.363758046624906	27.519270701188788	21.962951922424555
20-24	22.253487863830433	28.21394336845058	27.499725150077193	22.032843617641795
25-29	22.330605124320833	28.182798111072145	27.421157445619386	22.065439318987636
30-34	22.355032958165207	28.119984030976653	27.38241142285564	22.142571588002497
35-39	22.409367165262157	28.146112051162163	27.343446964183233	22.10107381939245
40-44	22.486074267999946	28.116075119842748	27.283050705945833	22.114799906211466
45-49	22.45504886901366	28.0908401399229	27.269259326318956	22.184851664744485
50-54	22.456823967719398	28.117898424410637	27.257460295970006	22.167817311899967
55-59	22.437282581737065	28.120696305938765	27.237262313144562	22.204758799179604
60-64	22.43382603936173	28.101899018010585	27.202450393237964	22.26182454938973
65-69	22.470214322310937	28.10833464995101	27.210637020818712	22.21081400691934
70-74	22.451911241338347	28.105078114885263	27.19269453955632	22.250316104220072
75-79	22.40182526881777	28.105109783590045	27.202475576643277	22.290589370948908
80-84	22.445387578838325	28.15779357065634	27.16296906173956	22.233849788765774
85-89	22.416907381020806	28.15820456097357	27.169837400328163	22.255050657677465
90-94	22.406090271185043	28.123320071491975	27.156688904191473	22.313900753131506
95-99	22.42124452993682	28.139100680791085	27.179776210313666	22.259878578958425
100-104	22.43073162942273	28.174774262811642	27.14860842187231	22.245885685893317
105-109	22.390654311046866	28.151255541535182	27.175332011829546	22.28275813558841
110-114	22.403569747932618	28.20795932257712	27.165262661560853	22.223208267929408
115-119	22.45245364161036	28.18291155686079	27.126687180826515	22.237947620702332
120-124	22.429979573488513	28.14801317226142	27.132580051479323	22.289427202770746
125-129	22.41900365786351	28.222619963175678	27.144359048013666	22.214017330947144
130-134	22.440910742708272	28.24681313783749	27.102366849618264	22.209909269835972
135-139	22.407846092046558	28.23810113557904	27.05739537537008	22.29665739700432
140-144	22.4007229858475	28.306507260078178	27.084023011241726	22.2087467428326
145-149	22.44977706744608	28.357564296709768	27.006875658824043	22.185782977020107
150	22.333407784657552	28.05010240322489	26.885308049489474	22.731181762628086
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	44.28212206362772
2	42.21490119533847
3	43.388457034595305
4	44.145922451484886
5	43.9964422552151
6	44.46375995333431
7	44.21929339579981
8	43.03299565049787
9	43.17078694765097
10-14	43.964441105232495
15-19	44.11697125218631
20-24	44.28633148147223
25-29	44.39604444330847
30-34	44.49760454616771
35-39	44.5104409846546
40-44	44.60087417421141
45-49	44.639900533758144
50-54	44.62464127961937
55-59	44.64204138091667
60-64	44.695650588751455
65-69	44.68102832923028
70-74	44.70222734555841
75-79	44.692414639766675
80-84	44.679237367604095
85-89	44.67195803869827
90-94	44.71999102431655
95-99	44.68112310889524
100-104	44.67661731531605
105-109	44.673412446635275
110-114	44.62677801586203
115-119	44.6904012623127
120-124	44.719406776259255
125-129	44.63302098881066
130-134	44.650820012544244
135-139	44.70450348905088
140-144	44.609469728680104
145-149	44.63556004446619
150	45.064589547285635
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	95.0
1	214.5
2	511.5
3	859.5
4	1176.0
5	1427.5
6	1607.0
7	1794.0
8	2076.0
9	2425.5
10	2770.5
11	3326.0
12	4016.5
13	4698.5
14	7814.0
15	11401.5
16	19312.5
17	36977.0
18	44577.0
19	69009.5
20	102147.0
21	116346.0
22	124937.0
23	132323.5
24	180493.0
25	254826.0
26	312129.5
27	373952.0
28	458715.5
29	528623.0
30	597095.5
31	681526.5
32	781945.5
33	944469.0
34	1156083.5
35	1413360.5
36	1688567.5
37	2010082.5
38	2311290.5
39	2596214.0
40	2964465.5
41	3400331.0
42	3828317.0
43	4221634.0
44	4563763.0
45	4782365.5
46	4957280.0
47	5012183.5
48	4858408.5
49	4646604.0
50	4375098.5
51	3848122.5
52	3195416.5
53	2717245.5
54	2288602.5
55	1815435.5
56	1393417.5
57	980841.5
58	640371.5
59	426320.0
60	310949.0
61	216996.5
62	123222.5
63	61034.0
64	23803.0
65	10420.5
66	4706.5
67	3019.0
68	2249.0
69	1889.5
70	1622.0
71	1406.0
72	1311.5
73	1156.5
74	1064.5
75	1043.0
76	1014.0
77	979.5
78	937.5
79	906.5
80	937.5
81	966.0
82	934.5
83	920.0
84	964.5
85	985.5
86	1021.0
87	1075.5
88	1145.5
89	1269.0
90	1410.0
91	1695.0
92	2180.5
93	2870.5
94	3838.5
95	8280.5
96	12588.0
97	10008.5
98	5052.5
99	3030.0
100	11560.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08039885760872892
2	0.05851243478967451
3	0.044997015828363456
4	0.026839901209828185
5	0.01737040702341604
6	0.022338386936680502
7	0.008054387283363568
8	0.004169187716069664
9	0.0034622384946491553
10-14	0.003701755307786839
15-19	0.0052809711070215745
20-24	0.010167259110255221
25-29	0.020837962742978448
30-34	0.028120627337796484
35-39	0.02905935922702631
40-44	0.028279177317027886
45-49	0.03470794392918655
50-54	0.030612713977819343
55-59	0.03485827637900657
60-64	0.031697911244205006
65-69	0.02449742194350081
70-74	0.023521711171919757
75-79	0.02610588247975841
80-84	0.038605228098556016
85-89	0.040562933634797425
90-94	0.051861553191366976
95-99	0.048239797949320366
100-104	0.04987750322260084
105-109	0.058478356211821424
110-114	0.059499263395165085
115-119	0.06951208129089459
120-124	0.061553645748011004
125-129	0.07147848774063041
130-134	0.06045491372730071
135-139	0.05604862611850848
140-144	0.06710966239827243
145-149	0.06561093004886094
150	0.07859341805863962
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	8.2749932E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	88.05993306292528
#Duplication Level	Relative count
1	100.0
2	20.26174122888435
3	11.957799641111317
4	8.43697790978281
5	7.165398180805643
6	5.91238165955077
7	5.225542973825877
8	4.532516552193552
9	4.117938246395644
10++	451.16329435059714
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE