##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	N0058.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	88077968	
Filtered Sequences	0	
Sequence length	150	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.088857261103026	32.0	32.0	32.0	27.0	32.0
2	30.87524823460959	32.0	32.0	32.0	27.0	32.0
3	30.988684548217552	32.0	32.0	32.0	32.0	32.0
4	30.962455321403418	32.0	32.0	32.0	27.0	32.0
5	30.88921566628331	32.0	32.0	32.0	27.0	32.0
6	35.318303028970874	37.0	37.0	37.0	32.0	37.0
7	35.2419153788834	37.0	37.0	37.0	32.0	37.0
8	35.134516125530965	37.0	37.0	37.0	32.0	37.0
9	35.12272050826604	37.0	37.0	37.0	32.0	37.0
10-14	35.06965095970425	37.0	37.0	37.0	32.0	37.0
15-19	34.996413173382926	37.0	37.0	37.0	32.0	37.0
20-24	34.94543927035192	37.0	37.0	37.0	32.0	37.0
25-29	34.928851426272686	37.0	37.0	37.0	32.0	37.0
30-34	34.89442278232395	37.0	37.0	37.0	32.0	37.0
35-39	34.83164718332285	37.0	37.0	37.0	32.0	37.0
40-44	34.76271153757771	37.0	37.0	37.0	29.0	37.0
45-49	34.687395671980084	37.0	37.0	37.0	27.0	37.0
50-54	34.62771912494621	37.0	37.0	37.0	27.0	37.0
55-59	34.5554936053929	37.0	37.0	37.0	27.0	37.0
60-64	34.48551134149689	37.0	37.0	37.0	27.0	37.0
65-69	34.40441468404448	37.0	37.0	37.0	27.0	37.0
70-74	34.320952229506474	37.0	37.0	37.0	27.0	37.0
75-79	34.2574476400273	37.0	37.0	37.0	27.0	37.0
80-84	34.163059997024455	37.0	37.0	37.0	27.0	37.0
85-89	34.05231211283167	37.0	37.0	37.0	27.0	37.0
90-94	33.91958366932353	37.0	37.0	37.0	27.0	37.0
95-99	33.77312341038567	37.0	37.0	37.0	27.0	37.0
100-104	33.633276875778975	37.0	33.0	37.0	22.0	37.0
105-109	33.476265465161504	37.0	32.0	37.0	22.0	37.0
110-114	33.3436522650023	37.0	32.0	37.0	22.0	37.0
115-119	33.193673936710255	37.0	32.0	37.0	22.0	37.0
120-124	33.08641129413885	37.0	32.0	37.0	22.0	37.0
125-129	32.96419898106641	37.0	32.0	37.0	22.0	37.0
130-134	32.865624483979914	37.0	32.0	37.0	22.0	37.0
135-139	32.778241382680406	37.0	32.0	37.0	22.0	37.0
140-144	32.64759545315577	37.0	32.0	37.0	22.0	37.0
145-149	32.39990867636728	37.0	32.0	37.0	18.4	37.0
150	30.617777183506323	37.0	27.0	37.0	13.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	105.0
5	1260.0
6	2220.0
7	1826.0
8	1661.0
9	1657.0
10	1715.0
11	4038.0
12	10874.0
13	17280.0
14	22555.0
15	29398.0
16	37842.0
17	46478.0
18	61911.0
19	96264.0
20	160437.0
21	248496.0
22	359836.0
23	493137.0
24	646513.0
25	821397.0
26	1022421.0
27	1261856.0
28	1560433.0
29	1964782.0
30	2535096.0
31	3430298.0
32	4999016.0
33	8176568.0
34	1.5648799E7
35	3.0655069E7
36	1.3756727E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.45377660931176	32.44964798738473	22.217874178029604	27.878701225273904
2	23.533106577924066	31.318988450501433	25.649909716309143	19.497995255265355
3	21.624378194756034	29.776701406844712	26.427967072527146	22.1709533258721
4	21.60405190057592	27.910545816380107	27.553749997600914	22.93165228544306
5	23.287326007944948	28.25972186253449	27.44708309319138	21.005869036329177
6	22.8274732071059	28.316596456048366	26.706586351701738	22.149343985143997
7	23.855129361120593	28.015451735117093	27.15359685130889	20.975822052453424
8	21.79731999005385	28.203210244078015	28.17530312474808	21.824166641120055
9	21.69064063409932	27.20857991462699	28.959623449625138	22.14115600164855
10-14	22.5713637186657	28.048910870519705	27.300421665841395	22.079303744973195
15-19	22.454232032300425	28.010825359274875	27.213783982460942	22.321158625963754
20-24	22.60728373607459	27.879835720691563	27.164571147674906	22.348309395558942
25-29	22.617323932066785	27.879705978880875	27.090831604766553	22.412138484285787
30-34	22.678846400798463	27.78068602436469	27.04955091691127	22.49091665792557
35-39	22.74007982785303	27.787313267754666	26.972757643879824	22.49984926051248
40-44	22.806655259006163	27.746792592892128	26.984855053843443	22.461697094258266
45-49	22.77773824098484	27.69431395472951	26.988038326909024	22.539909477376625
50-54	22.8344011743463	27.751993222021287	26.94912605734568	22.464479546286732
55-59	22.76318529355459	27.809404340111676	26.92564152937318	22.501768836960558
60-64	22.821691137367647	27.75110068063563	26.85860024047105	22.568607941525674
65-69	22.767963986904622	27.748542970970213	26.927724616718145	22.555768425407017
70-74	22.744361923036806	27.822985911954927	26.82454222839144	22.60810993661683
75-79	22.76021425741069	27.808812858166576	26.898702447169615	22.532270437253114
80-84	22.78004300713259	27.831964858314134	26.83024186662787	22.557750267925407
85-89	22.75459734993893	27.837643480289646	26.82157308813543	22.586186081635994
90-94	22.709030776966603	27.80811161589653	26.84409021933582	22.63876738780105
95-99	22.80572318163361	27.78629005197783	26.901883187035082	22.506103579353486
100-104	22.779884104981566	27.787337087633606	26.80056375662277	22.63221505076206
105-109	22.75162564458032	27.79206026940791	26.825603363493496	22.63071072251828
110-114	22.755569534154837	27.8187725083453	26.86482232404885	22.560835633451013
115-119	22.757972805605185	27.849318760613805	26.79671569071141	22.595992743069605
120-124	22.74306328828792	27.859827083418903	26.786772963783662	22.610336664509514
125-129	22.77490120245682	27.869754124525485	26.812028623889777	22.54331604912792
130-134	22.80877994763706	27.870257313015127	26.736392203738742	22.58457053560907
135-139	22.729813480691536	27.915058971404864	26.76783870138331	22.587288846520288
140-144	22.812928712973346	27.86829734773921	26.785713790687854	22.533060148599585
145-149	22.8586059240434	27.884430067921716	26.73925819654888	22.517705811486
150	22.821049633288666	27.46498499324795	26.71199251986617	23.001972853597223
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	45.33247783458567
2	43.031101833189425
3	43.79533152062814
4	44.53570418601898
5	44.29319504427413
6	44.9768171922499
7	44.83095141357401
8	43.62148663117391
9	43.83179663574787
10-14	44.6506674636389
15-19	44.77539065826418
20-24	44.955593131633535
25-29	45.02946241635257
30-34	45.16976305872404
35-39	45.23992908836551
40-44	45.26835235326443
45-49	45.31764771836146
50-54	45.298880720633036
55-59	45.26495413051514
60-64	45.39029907889332
65-69	45.32373241231164
70-74	45.35247185965363
75-79	45.292484694663806
80-84	45.337793275058
85-89	45.34078343157493
90-94	45.34779816476765
95-99	45.31182676098709
100-104	45.412099155743626
105-109	45.382336367098596
110-114	45.31640516760585
115-119	45.35396554867479
120-124	45.35339995279743
125-129	45.31821725158474
130-134	45.39335048324613
135-139	45.31710232721182
140-144	45.34598886157293
145-149	45.3763117355294
150	45.82302248688589
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	158.0
1	274.0
2	567.0
3	944.5
4	1233.0
5	1424.0
6	1593.5
7	1717.5
8	1923.0
9	2205.0
10	2528.0
11	3345.5
12	4577.5
13	6054.5
14	8923.0
15	13250.0
16	15104.0
17	17554.5
18	22760.0
19	31149.5
20	48546.0
21	70610.5
22	100223.5
23	147522.0
24	179799.5
25	201468.0
26	234026.5
27	287108.0
28	373467.5
29	491984.0
30	610954.5
31	691995.5
32	778877.5
33	984641.0
34	1252805.0
35	1391577.5
36	1548244.5
37	1768432.0
38	2020527.5
39	2355600.5
40	2686514.0
41	3122358.0
42	3677652.5
43	4183158.0
44	4686968.0
45	5291679.5
46	5884184.5
47	6097156.0
48	5848529.0
49	5391402.0
50	4911916.0
51	4280821.5
52	3534257.0
53	2894369.0
54	2389426.0
55	1964246.0
56	1544078.5
57	1207982.5
58	927025.0
59	645157.0
60	409058.5
61	255674.0
62	165893.5
63	104052.0
64	68464.0
65	60247.5
66	38801.0
67	20818.5
68	12088.5
69	5307.5
70	2871.0
71	1927.0
72	1361.5
73	1167.5
74	1060.0
75	1024.5
76	986.0
77	964.0
78	920.5
79	886.0
80	854.5
81	850.0
82	857.0
83	842.0
84	862.0
85	894.5
86	897.5
87	935.0
88	1025.0
89	1131.0
90	1263.0
91	1528.5
92	2027.0
93	2763.5
94	3780.0
95	8625.5
96	13450.5
97	10832.5
98	5294.0
99	2615.5
100	9858.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0812972887839556
2	0.05852655456356577
3	0.04513160430767431
4	0.02689094734792247
5	0.017563983764929725
6	0.022508466589510785
7	0.008334660944948231
8	0.0041644920782005325
9	0.0034730592331557873
10-14	0.00374395558262652
15-19	0.005248985762251009
20-24	0.010143285776075124
25-29	0.0205690485502572
30-34	0.027850097540851534
35-39	0.028830365387176052
40-44	0.028088522659832478
45-49	0.03472605090072014
50-54	0.030585401334417704
55-59	0.03470266253190582
60-64	0.03173302090711266
65-69	0.02447263542683001
70-74	0.023482603504204367
75-79	0.02614206540278041
80-84	0.03888214133187087
85-89	0.04086947146646253
90-94	0.052297982169615904
95-99	0.04852518850116978
100-104	0.04990033375883513
105-109	0.0587856431928584
110-114	0.05973888952569841
115-119	0.06991555481843087
120-124	0.062199663825123666
125-129	0.0720171019385915
130-134	0.060914893041129195
135-139	0.05640093785996516
140-144	0.06764415818493906
145-149	0.06604057895613577
150	0.07938080497043257
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	8.8077968E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	89.17850075199873
#Duplication Level	Relative count
1	100.0
2	19.100564707259267
3	12.11633554729789
4	9.717061181496328
5	8.43257161248793
6	7.36752786961992
7	6.723820112941452
8	6.53363373028645
9	5.843110864031366
10++	409.35424408227755
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE