##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	N0059.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	88976978	
Filtered Sequences	0	
Sequence length	150	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.013949349909367	32.0	27.0	32.0	27.0	32.0
2	30.394576313886496	32.0	32.0	32.0	27.0	32.0
3	30.660507811357675	32.0	32.0	32.0	27.0	32.0
4	30.846827389439998	32.0	32.0	32.0	27.0	32.0
5	30.796372135722567	32.0	32.0	32.0	27.0	32.0
6	35.2626241475632	37.0	37.0	37.0	32.0	37.0
7	35.20715877763347	37.0	37.0	37.0	32.0	37.0
8	35.06590838587483	37.0	37.0	37.0	32.0	37.0
9	35.0676485438739	37.0	37.0	37.0	32.0	37.0
10-14	35.04110755481041	37.0	37.0	37.0	32.0	37.0
15-19	34.98533255872098	37.0	37.0	37.0	32.0	37.0
20-24	34.94326063535221	37.0	37.0	37.0	32.0	37.0
25-29	34.93432616693275	37.0	37.0	37.0	32.0	37.0
30-34	34.892404221685304	37.0	37.0	37.0	32.0	37.0
35-39	34.826262777771575	37.0	37.0	37.0	32.0	37.0
40-44	34.75891374283357	37.0	37.0	37.0	29.0	37.0
45-49	34.68893233258608	37.0	37.0	37.0	27.0	37.0
50-54	34.62559902630094	37.0	37.0	37.0	27.0	37.0
55-59	34.556393585315966	37.0	37.0	37.0	27.0	37.0
60-64	34.48622446583879	37.0	37.0	37.0	27.0	37.0
65-69	34.40740347463812	37.0	37.0	37.0	27.0	37.0
70-74	34.32316666677531	37.0	37.0	37.0	27.0	37.0
75-79	34.26122515197134	37.0	37.0	37.0	27.0	37.0
80-84	34.16844092637086	37.0	37.0	37.0	27.0	37.0
85-89	34.057578354706536	37.0	37.0	37.0	27.0	37.0
90-94	33.92191385506485	37.0	37.0	37.0	27.0	37.0
95-99	33.78142240794018	37.0	37.0	37.0	27.0	37.0
100-104	33.64088043313856	37.0	33.0	37.0	22.0	37.0
105-109	33.48974216004503	37.0	32.0	37.0	22.0	37.0
110-114	33.35163416541299	37.0	32.0	37.0	22.0	37.0
115-119	33.21079759305829	37.0	32.0	37.0	22.0	37.0
120-124	33.105878073314656	37.0	32.0	37.0	22.0	37.0
125-129	32.97614382677731	37.0	32.0	37.0	22.0	37.0
130-134	32.881804562973585	37.0	32.0	37.0	22.0	37.0
135-139	32.79608311489294	37.0	32.0	37.0	22.0	37.0
140-144	32.67012083058159	37.0	32.0	37.0	22.0	37.0
145-149	32.42316683535824	37.0	32.0	37.0	20.2	37.0
150	30.627206489301088	37.0	27.0	37.0	13.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	123.0
5	1354.0
6	2226.0
7	1840.0
8	1631.0
9	1732.0
10	1765.0
11	4625.0
12	12456.0
13	19130.0
14	26043.0
15	33829.0
16	42207.0
17	49954.0
18	64208.0
19	96131.0
20	157868.0
21	241383.0
22	350344.0
23	481416.0
24	642061.0
25	826967.0
26	1041874.0
27	1292678.0
28	1602741.0
29	2010590.0
30	2584307.0
31	3479253.0
32	5048280.0
33	8230628.0
34	1.5756045E7
35	3.1071925E7
36	1.3799358E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.05332041754936	33.242043052130846	22.925634276543764	26.779002253776035
2	22.804862153121935	32.522535912547376	25.749992243546714	18.922609690783972
3	20.762031512998316	31.05079169283339	26.398423355005768	21.788753439162527
4	20.768384589153136	28.84718580155143	28.050455874717432	22.333973734578
5	22.636464631781227	28.990929493144314	28.048144341434362	20.324461533640108
6	22.06395726730431	29.182456115580408	27.3345909135241	21.418995703591186
7	23.160600670488353	28.81537930779146	27.859643529738833	20.164376491981358
8	21.261293322720856	28.912010991706822	28.861085579407952	20.965610106164366
9	21.022942868327835	28.03353854589507	29.597803617133977	21.345714968643115
10-14	21.79934458997203	28.87019770773371	27.987301745407915	21.34315595688634
15-19	21.76421640567857	28.783289966144803	27.93527422609696	21.517219402079665
20-24	21.87042851049857	28.590796534056885	27.91791828432551	21.62085667111904
25-29	21.962590048588307	28.553130908300883	27.834351031846033	21.649928011264777
30-34	21.99061828934213	28.51945207787215	27.767018176423047	21.722911456362677
35-39	22.073736016585674	28.479635544373238	27.731938482527056	21.71468995651403
40-44	22.070788936388915	28.498523543262877	27.710373730960015	21.720313789388193
45-49	22.077533831892552	28.473876147584125	27.671786021193046	21.77680399933028
50-54	22.085840421966022	28.4846002303193	27.674946091552634	21.75461325616205
55-59	22.078974914396003	28.498368177243805	27.654721171685008	21.76793573667518
60-64	22.042733276123986	28.474168869263462	27.651305445266637	21.83179240934592
65-69	22.05428371256327	28.51058661430448	27.64820514034374	21.78692453278851
70-74	22.06879842232567	28.529138282631166	27.63108766213236	21.770975632910805
75-79	21.991270050118526	28.50283774937097	27.656159044683946	21.849733155826556
80-84	22.054336878127987	28.51660680063366	27.62907402337739	21.79998229786096
85-89	22.054141189422754	28.532529774434977	27.612692407004246	21.800636629138022
90-94	22.005663378295058	28.53988647839511	27.603980839558922	21.850469303750913
95-99	22.019649003247157	28.532401146445224	27.623956331670325	21.82399351863729
100-104	22.051391030850194	28.57000181774716	27.608169589780662	21.770437561621982
105-109	22.032292556771036	28.522993127235058	27.60059919505683	21.844115120937076
110-114	22.011806010776485	28.607631178977705	27.5929247097771	21.787638100468712
115-119	22.028402883163455	28.60880165624804	27.57652643132602	21.78626902926248
120-124	22.03578363107428	28.545523321079475	27.5924779044534	21.826215143392844
125-129	22.022699971832957	28.60239913253907	27.60469323215757	21.770207663470405
130-134	22.06913821260637	28.63365593746344	27.555833128234	21.74137272169619
135-139	22.021229132015634	28.60060913859659	27.58507804956406	21.79308367982372
140-144	22.08394894449347	28.612470391874016	27.54738163314664	21.756199030485877
145-149	22.096142354755383	28.670138390487498	27.51915602053186	21.714563234225256
150	22.088299962476718	28.246399146654355	27.584205047704263	22.08109584316467
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.8323226713254
2	41.7274718439059
3	42.550784952160846
4	43.10235832373114
5	42.960926165421334
6	43.4829529708955
7	43.32497716246971
8	42.22690342888523
9	42.368657836970954
10-14	43.14250054685837
15-19	43.28143580775824
20-24	43.491285181617606
25-29	43.61251805985308
30-34	43.713529745704804
35-39	43.788425973099706
40-44	43.79110272577711
45-49	43.85433783122283
50-54	43.840453678128064
55-59	43.846910651071184
60-64	43.8745256854699
65-69	43.84120824535178
70-74	43.839774055236475
75-79	43.841003205945086
80-84	43.85431917598895
85-89	43.85477781856078
90-94	43.85613268204597
95-99	43.843642521884455
100-104	43.82182859247218
105-109	43.876407677708116
110-114	43.799444111245194
115-119	43.814671912425936
120-124	43.861998774467125
125-129	43.792907635303365
130-134	43.81051093430256
135-139	43.81431281183935
140-144	43.840147974979345
145-149	43.81070558898064
150	44.169395805641386
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	362.0
1	349.0
2	463.5
3	717.0
4	970.0
5	1211.5
6	1407.5
7	1584.5
8	1799.0
9	2095.5
10	2428.5
11	2811.0
12	3255.5
13	4008.0
14	4913.5
15	5951.0
16	7873.5
17	11356.5
18	17101.0
19	23909.0
20	38081.0
21	61918.0
22	95129.0
23	132407.5
24	158561.0
25	204041.0
26	264131.5
27	315414.0
28	396991.5
29	518436.0
30	693773.0
31	911680.0
32	1091183.5
33	1257505.5
34	1480087.0
35	1754082.5
36	2092515.5
37	2471164.0
38	2905348.5
39	3390075.0
40	3876337.0
41	4376606.0
42	4892798.5
43	5316352.0
44	5533119.0
45	5601771.0
46	5517485.5
47	5290736.5
48	4974297.0
49	4542694.0
50	4027525.0
51	3448623.5
52	2803733.5
53	2219552.0
54	1761932.0
55	1379221.5
56	1025479.5
57	705466.0
58	468838.5
59	304572.0
60	184005.0
61	107639.5
62	69396.0
63	43478.0
64	27505.0
65	17947.5
66	9771.0
67	4868.0
68	3062.5
69	2250.0
70	1836.0
71	1597.5
72	1526.0
73	1452.5
74	1384.5
75	1289.5
76	1226.0
77	1219.5
78	1177.0
79	1145.0
80	1144.0
81	1139.5
82	1104.0
83	1127.5
84	1148.5
85	1140.0
86	1183.0
87	1253.0
88	1351.0
89	1443.0
90	1631.0
91	1977.0
92	2494.0
93	3240.5
94	4238.0
95	9422.0
96	15302.0
97	13010.5
98	6664.0
99	3495.0
100	13600.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07838094928330787
2	0.057346294678607766
3	0.04412939266154892
4	0.026267468872678505
5	0.017134769400686995
6	0.02235859257885787
7	0.008091980826770718
8	0.004177485101820384
9	0.003433472420247853
10-14	0.0037101732090743743
15-19	0.005218878078776737
20-24	0.009943470995384895
25-29	0.020141165055077508
30-34	0.0270573361122694
35-39	0.028025901261784815
40-44	0.02744799896440628
45-49	0.03390270233722705
50-54	0.030036533719992153
55-59	0.0341317503500737
60-64	0.03133304887023697
65-69	0.024262905400091246
70-74	0.023290294260162443
75-79	0.02576823861111579
80-84	0.03827327109266399
85-89	0.040209502282714076
90-94	0.05141509750983002
95-99	0.04787080990770444
100-104	0.0492457723165199
105-109	0.057930490738851564
110-114	0.05867517775215966
115-119	0.06868900402528842
120-124	0.06108681281578253
125-129	0.07096307541485619
130-134	0.06001619879695173
135-139	0.055630794743332365
140-144	0.06658239168338578
145-149	0.06514246865071098
150	0.07812245545134158
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	8.8976978E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	87.44509789324798
#Duplication Level	Relative count
1	100.0
2	18.79632374740587
3	11.05511084758046
4	8.241921138452415
5	6.769443620911157
6	6.26544124913529
7	5.5802615541720195
8	5.198142108904042
9	5.257436505583556
10++	491.66254900023057
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	19988445	3.0059526	3.5940325	6
>>END_MODULE