##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	N0060.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	95112246	
Filtered Sequences	0	
Sequence length	150	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.649110746475277	32.0	27.0	32.0	27.0	32.0
2	30.825604244483934	32.0	32.0	32.0	27.0	32.0
3	30.819197719292635	32.0	32.0	32.0	27.0	32.0
4	30.87799698263881	32.0	32.0	32.0	27.0	32.0
5	30.76888982308335	32.0	32.0	32.0	27.0	32.0
6	35.08178694465905	37.0	37.0	37.0	32.0	37.0
7	35.03609736016538	37.0	37.0	37.0	32.0	37.0
8	34.958079877537536	37.0	37.0	37.0	32.0	37.0
9	34.968531381332326	37.0	37.0	37.0	32.0	37.0
10-14	34.8851131094097	37.0	37.0	37.0	32.0	37.0
15-19	34.804044295200434	37.0	37.0	37.0	28.0	37.0
20-24	34.74841981967286	37.0	37.0	37.0	27.0	37.0
25-29	34.73624011149941	37.0	37.0	37.0	27.0	37.0
30-34	34.69038569859869	37.0	37.0	37.0	27.0	37.0
35-39	34.61771402601511	37.0	37.0	37.0	27.0	37.0
40-44	34.54322618141096	37.0	37.0	37.0	27.0	37.0
45-49	34.46406876775889	37.0	37.0	37.0	27.0	37.0
50-54	34.393278304036684	37.0	37.0	37.0	27.0	37.0
55-59	34.31928188090522	37.0	37.0	37.0	27.0	37.0
60-64	34.24363445901593	37.0	37.0	37.0	27.0	37.0
65-69	34.15416949358971	37.0	37.0	37.0	27.0	37.0
70-74	34.05951351206657	37.0	37.0	37.0	27.0	37.0
75-79	33.98593042372272	37.0	37.0	37.0	27.0	37.0
80-84	33.88553304271671	37.0	37.0	37.0	23.0	37.0
85-89	33.76811723697493	37.0	37.0	37.0	22.0	37.0
90-94	33.62649678149752	37.0	36.0	37.0	22.0	37.0
95-99	33.47475638625966	37.0	32.0	37.0	22.0	37.0
100-104	33.32331001625174	37.0	32.0	37.0	22.0	37.0
105-109	33.159429020317745	37.0	32.0	37.0	22.0	37.0
110-114	33.014442365287	37.0	32.0	37.0	22.0	37.0
115-119	32.865308995016264	37.0	32.0	37.0	22.0	37.0
120-124	32.74910640423737	37.0	32.0	37.0	22.0	37.0
125-129	32.61449418826678	37.0	32.0	37.0	22.0	37.0
130-134	32.51337589062926	37.0	32.0	37.0	22.0	37.0
135-139	32.428783450240466	37.0	32.0	37.0	14.8	37.0
140-144	32.29328617473716	37.0	32.0	37.0	13.0	37.0
145-149	32.0365026980858	37.0	31.0	37.0	13.0	37.0
150	30.292371688920056	37.0	27.0	37.0	13.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	103.0
5	1359.0
6	2424.0
7	1941.0
8	1741.0
9	1713.0
10	1937.0
11	4292.0
12	11681.0
13	18239.0
14	24833.0
15	33252.0
16	42627.0
17	52432.0
18	69955.0
19	113699.0
20	193639.0
21	307967.0
22	450906.0
23	624675.0
24	829234.0
25	1064892.0
26	1325225.0
27	1629821.0
28	2001002.0
29	2486802.0
30	3167950.0
31	4208158.0
32	5993095.0
33	9511659.0
34	1.7558557E7
35	3.1510077E7
36	1.1866357E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.122757853744446	31.649376904929582	22.16598758417619	28.06187765714978
2	24.498901411774447	31.41740562333089	25.621141875303188	18.462551089591475
3	20.39665003407099	29.74007992035607	26.902688281222176	22.96058176435076
4	21.646382049122558	27.146619107363247	28.143462628669113	23.063536214845083
5	23.71721796057797	27.967159464059478	27.670501590912867	20.64512098444969
6	22.398516584663845	28.15897616545225	26.698824901449754	22.743682348434156
7	24.321157849221244	27.79663686270571	27.14972407238932	20.732481215683727
8	22.211356125807914	27.621971184564686	28.1268179611271	22.0398547285003
9	22.07110634259325	26.72733419168898	28.87486843514173	22.32669103057604
10-14	22.762986014361854	27.86294401903661	26.998922786030597	22.375147180570938
15-19	22.67708649627742	27.74589883776011	27.03927303790556	22.537741628056917
20-24	22.839969327096256	27.646449700390225	26.944559105441137	22.56902186707239
25-29	22.843187611067066	27.683038599082288	26.864977071708456	22.60879671814219
30-34	22.874889098275098	27.54989138390137	26.78530927443977	22.789910243383765
35-39	22.939139234203182	27.578236456100548	26.75397359311108	22.728650716585193
40-44	22.9898893364774	27.582194363649197	26.704984380769815	22.722931919103587
45-49	22.982303568809403	27.53869681816888	26.699399813450757	22.779599799570967
50-54	22.969335154354802	27.570407875180315	26.685945559517915	22.774311410946975
55-59	22.99198579568035	27.59362703150937	26.631352377537244	22.78303479527304
60-64	22.961780606007316	27.573544425078712	26.6142875787162	22.850387390197774
65-69	22.96566619671277	27.636916415788683	26.612583741357476	22.784833646141074
70-74	22.970564614258254	27.648898483314422	26.609259877549295	22.771277024878028
75-79	22.92473901311825	27.6401029438192	26.595249096054417	22.839908947008134
80-84	22.933109160791297	27.659949676875474	26.623811215446235	22.78312994688699
85-89	22.94604720687487	27.72445978883058	26.55558399243339	22.773909011861157
90-94	22.87303340446349	27.722904152375182	26.596961796440976	22.807100646720347
95-99	22.88936230825223	27.7817234611908	26.56627482563328	22.762639404923696
100-104	22.887266021023795	27.79975564603005	26.541560355846507	22.771417977099652
105-109	22.841710941926465	27.793582633727205	26.55306643711844	22.811639987227892
110-114	22.87639764308588	27.802778367333435	26.53903373243927	22.78179025714141
115-119	22.86137579274272	27.84397922325167	26.5036095875299	22.79103539647571
120-124	22.87837033084799	27.79224588695322	26.49985625989877	22.82952752230002
125-129	22.89068583888934	27.845976931928007	26.49866831445263	22.76466891473002
130-134	22.85516270235287	27.871533185268284	26.510293271600965	22.763010840777877
135-139	22.86663444320475	27.81839878883411	26.501437202673937	22.8135295652872
140-144	22.883647725512112	27.926852455773933	26.448092234092478	22.741407584621477
145-149	22.904938709486014	27.925044082538918	26.442702292276454	22.727314915698614
150	22.906875067019627	27.48703855813317	26.31341079941963	23.29267557542758
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	46.18463551089423
2	42.961452501365926
3	43.35723179842175
4	44.70991826396764
5	44.36233894502766
6	45.142198933098
7	45.05363906490497
8	44.25121085430821
9	44.39779737316929
10-14	45.13813319493279
15-19	45.21482812433434
20-24	45.40899119416865
25-29	45.45198432920925
30-34	45.66479934165886
35-39	45.667789950788375
40-44	45.71282125558099
45-49	45.761903368380366
50-54	45.74364656530177
55-59	45.77502059095339
60-64	45.81216799620509
65-69	45.75049984285384
70-74	45.74184163913628
75-79	45.76464796012638
80-84	45.716239107678284
85-89	45.71995621873602
90-94	45.68013405118384
95-99	45.65200171317593
100-104	45.65868399812344
105-109	45.65335092915436
110-114	45.65818790022729
115-119	45.65241118921843
120-124	45.70789785314801
125-129	45.655354753619356
130-134	45.61817354313075
135-139	45.68016400849195
140-144	45.62505531013359
145-149	45.632253625184624
150	46.19955064244721
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	121.0
1	269.5
2	587.5
3	929.5
4	1198.0
5	1387.0
6	1529.5
7	1698.5
8	1935.5
9	2247.0
10	2624.5
11	3010.5
12	3469.5
13	4057.0
14	4823.0
15	7976.0
16	12230.5
17	15742.5
18	20007.5
19	22398.5
20	25331.0
21	34202.0
22	57602.0
23	111277.0
24	157649.5
25	193481.0
26	251624.0
27	307242.5
28	355679.0
29	383474.0
30	453670.0
31	589743.0
32	733551.0
33	937963.0
34	1164786.0
35	1362934.0
36	1621909.5
37	1989725.0
38	2335354.0
39	2639144.5
40	3095772.0
41	3594507.5
42	4061726.5
43	4686844.5
44	5246781.0
45	5514522.5
46	5632382.0
47	5581824.0
48	5465865.5
49	5443802.0
50	5312153.0
51	4953999.5
52	4413016.5
53	3804320.5
54	3258012.0
55	2641583.5
56	2008214.5
57	1495798.0
58	1066598.5
59	732889.5
60	480531.5
61	304467.0
62	190944.0
63	110398.5
64	51995.5
65	24737.0
66	13089.0
67	6491.5
68	4089.0
69	2960.5
70	2234.0
71	1897.0
72	1658.0
73	1496.5
74	1418.5
75	1236.5
76	1075.5
77	984.0
78	968.5
79	954.5
80	931.0
81	936.5
82	927.0
83	912.5
84	924.5
85	950.5
86	991.5
87	1055.0
88	1129.0
89	1242.0
90	1427.0
91	1725.5
92	2277.0
93	3028.0
94	3948.0
95	8435.0
96	12568.0
97	9540.0
98	4388.0
99	2738.5
100	12465.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08086130149844217
2	0.058564488110185095
3	0.045314879852590174
4	0.02723939459909295
5	0.017708550379516852
6	0.022513399588944626
7	0.00817244921332212
8	0.004187683676400619
9	0.00342963197399418
10-14	0.0037479926612184094
15-19	0.005307203028304053
20-24	0.010172401984913699
25-29	0.020832438338171513
30-34	0.028082188280991703
35-39	0.02914913816670884
40-44	0.028276064472286776
45-49	0.03470383824181799
50-54	0.030547275689399658
55-59	0.03481444439867396
60-64	0.031514764145092314
65-69	0.024245248082986077
70-74	0.02316168624595407
75-79	0.02575630481904507
80-84	0.03855549789035578
85-89	0.040496993415548195
90-94	0.051868399785239015
95-99	0.048120827679750094
100-104	0.04980368143130591
105-109	0.05860044562505652
110-114	0.05957739658466271
115-119	0.06955024487593321
120-124	0.061762814432959556
125-129	0.07169087353903934
130-134	0.06058946394768136
135-139	0.05631072995584607
140-144	0.06740646204485594
145-149	0.0656910152242646
150	0.07863025335349562
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	9.5112246E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	87.98626013000998
#Duplication Level	Relative count
1	100.0
2	18.771264066998167
3	10.167495420047109
4	7.412980895053651
5	6.094608741167233
6	5.204789322166972
7	4.890735409578644
8	4.236456425019628
9	3.9780162261188172
10++	493.5226380528657
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE