##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	N0061.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	94393488	
Filtered Sequences	0	
Sequence length	150	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.37973903454018	32.0	32.0	32.0	27.0	32.0
2	30.79644748375015	32.0	32.0	32.0	27.0	32.0
3	30.85424479705634	32.0	32.0	32.0	27.0	32.0
4	30.927369025710757	32.0	32.0	32.0	27.0	32.0
5	30.857203878301437	32.0	32.0	32.0	27.0	32.0
6	35.20417458246696	37.0	37.0	37.0	32.0	37.0
7	35.13218922474822	37.0	37.0	37.0	32.0	37.0
8	35.08982956536154	37.0	37.0	37.0	32.0	37.0
9	35.086597658092685	37.0	37.0	37.0	32.0	37.0
10-14	34.99468665253688	37.0	37.0	37.0	32.0	37.0
15-19	34.921426238640535	37.0	37.0	37.0	32.0	37.0
20-24	34.870228702641015	37.0	37.0	37.0	32.0	37.0
25-29	34.8666218627285	37.0	37.0	37.0	32.0	37.0
30-34	34.815690864183345	37.0	37.0	37.0	32.0	37.0
35-39	34.75284397796594	37.0	37.0	37.0	27.0	37.0
40-44	34.682458609856646	37.0	37.0	37.0	27.0	37.0
45-49	34.615418377166016	37.0	37.0	37.0	27.0	37.0
50-54	34.549483188925066	37.0	37.0	37.0	27.0	37.0
55-59	34.48075467027981	37.0	37.0	37.0	27.0	37.0
60-64	34.40977669773152	37.0	37.0	37.0	27.0	37.0
65-69	34.32836111745336	37.0	37.0	37.0	27.0	37.0
70-74	34.24030622324286	37.0	37.0	37.0	27.0	37.0
75-79	34.17620819987074	37.0	37.0	37.0	27.0	37.0
80-84	34.08042979829287	37.0	37.0	37.0	27.0	37.0
85-89	33.960791594013344	37.0	37.0	37.0	27.0	37.0
90-94	33.82304836748908	37.0	37.0	37.0	27.0	37.0
95-99	33.67599486947659	37.0	34.0	37.0	23.0	37.0
100-104	33.528438023182275	37.0	32.0	37.0	22.0	37.0
105-109	33.368752005434956	37.0	32.0	37.0	22.0	37.0
110-114	33.22709850916834	37.0	32.0	37.0	22.0	37.0
115-119	33.08220717090145	37.0	32.0	37.0	22.0	37.0
120-124	32.970811958977514	37.0	32.0	37.0	22.0	37.0
125-129	32.837802385266244	37.0	32.0	37.0	22.0	37.0
130-134	32.737825507623995	37.0	32.0	37.0	22.0	37.0
135-139	32.65293135475617	37.0	32.0	37.0	22.0	37.0
140-144	32.515511885735165	37.0	32.0	37.0	22.0	37.0
145-149	32.260817693271385	37.0	32.0	37.0	16.6	37.0
150	30.408538044488832	37.0	27.0	37.0	13.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	98.0
5	1324.0
6	2520.0
7	2092.0
8	1837.0
9	1836.0
10	2150.0
11	6965.0
12	17111.0
13	26917.0
14	36869.0
15	49415.0
16	58448.0
17	63504.0
18	74375.0
19	106226.0
20	165698.0
21	253539.0
22	366211.0
23	508737.0
24	676724.0
25	873935.0
26	1100178.0
27	1374442.0
28	1721881.0
29	2181776.0
30	2835654.0
31	3850020.0
32	5624559.0
33	9259907.0
34	1.7937768E7
35	3.3329021E7
36	1.188175E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	16.515912184578045	33.436008002582746	24.728058515284058	25.32002129755515
2	22.35107825419041	33.178626168148014	27.526548902866544	16.943746674795037
3	18.8490283741403	31.59861823169307	28.499529361760406	21.05282403240622
4	19.90005532736296	28.983185731353807	29.98534737802651	21.131411563256727
5	21.894670380429723	29.656364389228163	29.516194977794846	18.932770252547268
6	20.709757782440004	29.866299519830562	28.47220980088859	20.95173289684084
7	22.275733477530558	29.620598781743514	29.01426651748987	19.089401223236063
8	20.325989606613984	29.561915769591668	29.897183107887766	20.214911515906582
9	20.393914743517097	28.463786764407917	30.736544592127075	20.40575389994791
10-14	20.89008793687331	29.700483209174994	28.896876650084785	20.51255220386691
15-19	20.785832880325174	29.600324119857373	28.94735924284034	20.666483756977115
20-24	21.013072862137427	29.48426748133913	28.793204956250033	20.7094547002734
25-29	20.99016897354799	29.505654960462323	28.723137656454988	20.7810384095347
30-34	21.073315931033477	29.38331969547359	28.687959248266797	20.855405125226138
35-39	21.11323448655703	29.4014430626513	28.61921730364359	20.866105147148087
40-44	21.131922244707145	29.39966600038189	28.593434775419468	20.874976979491493
45-49	21.109049029319984	29.348298952157155	28.62113228419721	20.921519734325653
50-54	21.128394193166457	29.408023713251154	28.567787724402848	20.895794369179537
55-59	21.117581699268964	29.412481428090842	28.597126573677023	20.87281029896317
60-64	21.097929677250704	29.397097915137564	28.557660290185172	20.947312117426556
65-69	21.181337763163814	29.416244971816198	28.539165629808004	20.86325163521198
70-74	21.100903414633812	29.435591670809423	28.54738154635928	20.916123368197486
75-79	21.083013197160874	29.462320009237015	28.510273420988874	20.94439337261324
80-84	21.12217093495116	29.41135338414825	28.56359472730453	20.90288095359606
85-89	21.09976761081389	29.47563016759857	28.540803046508316	20.883799175079222
90-94	21.107839408266358	29.427212968917203	28.551368968725054	20.91357865409139
95-99	21.100261829716267	29.47054875221103	28.522682133791154	20.90650728428155
100-104	21.089996373167274	29.513832683460883	28.527454887586	20.86871605578584
105-109	21.083366358678163	29.47491323572502	28.50498276184063	20.936737643756185
110-114	21.086874079513926	29.507648627507233	28.50530055197803	20.900176741000813
115-119	21.096028731416425	29.540886327804632	28.512249996384355	20.850834944394588
120-124	21.032101675974328	29.52342202573113	28.53756944046271	20.90690685783183
125-129	21.101635611222864	29.529537435102505	28.522509251118567	20.846317702556064
130-134	21.060259018734214	29.57058366173736	28.512838714911226	20.8563186046172
135-139	21.07556744637963	29.534941768954848	28.49355424663097	20.89593653803455
140-144	21.068127468115062	29.61988389678683	28.453419885785934	20.858568749312177
145-149	21.075064891043066	29.659372970198667	28.483454919347245	20.78210721941102
150	21.088356403354354	29.2120805151242	28.470479336114707	21.229083745406733
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.8359334821332
2	39.29482492898544
3	39.901852406546524
4	41.03146689061969
5	40.827440632976995
6	41.661490679280845
7	41.36513470076662
8	40.540901122520566
9	40.79966864346501
10-14	41.40264014074022
15-19	41.45231663730229
20-24	41.72252756241083
25-29	41.77120738308269
30-34	41.92872105625962
35-39	41.979339633705116
40-44	42.006899224198634
45-49	42.03056876364564
50-54	42.024188562346
55-59	41.990391998232134
60-64	42.04524179467726
65-69	42.0445893983758
70-74	42.017026782831294
75-79	42.027406569774115
80-84	42.025051888547225
85-89	41.98356678589311
90-94	42.021418062357746
95-99	42.00676911399782
100-104	41.95871242895311
105-109	42.020104002434344
110-114	41.98705082051474
115-119	41.94686367581102
120-124	41.93900853380616
125-129	41.94795331377893
130-134	41.916577623351415
135-139	41.971503984414184
140-144	41.926696217427235
145-149	41.85717211045408
150	42.31744014876109
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	174.0
1	295.5
2	599.5
3	951.5
4	1262.5
5	1526.5
6	1783.5
7	2006.0
8	2274.0
9	2697.0
10	3219.5
11	3728.5
12	4120.0
13	4640.5
14	5378.5
15	6185.0
16	7326.0
17	12613.5
18	19206.5
19	30992.5
20	46824.0
21	60860.0
22	101594.5
23	161932.0
24	253269.5
25	365053.0
26	477837.0
27	723286.5
28	1117191.0
29	1434933.0
30	1592097.5
31	1700006.5
32	1840155.0
33	2045019.5
34	2350929.5
35	2635559.5
36	2990317.0
37	3367118.5
38	3715715.0
39	4282116.5
40	4698711.5
41	4833144.5
42	4972167.0
43	5149917.5
44	5322844.0
45	5304253.0
46	5029300.0
47	4752771.5
48	4419581.5
49	3893126.5
50	3312473.5
51	2751345.5
52	2220377.0
53	1755717.5
54	1365311.0
55	1035562.5
56	743690.5
57	485841.5
58	316662.5
59	206524.5
60	125491.0
61	78228.5
62	47391.0
63	27526.0
64	16171.0
65	9475.0
66	6157.0
67	4417.0
68	3181.5
69	2511.5
70	2167.0
71	1923.0
72	1713.5
73	1512.5
74	1444.5
75	1380.0
76	1292.0
77	1223.5
78	1194.5
79	1215.0
80	1215.0
81	1138.0
82	1118.0
83	1153.0
84	1179.0
85	1191.0
86	1230.0
87	1335.5
88	1462.5
89	1599.0
90	1858.5
91	2285.0
92	3021.5
93	4056.5
94	5397.5
95	13945.5
96	22222.5
97	16427.5
98	6587.0
99	2949.5
100	12944.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08309577457292393
2	0.0605952817423168
3	0.04656147466443872
4	0.027625846393132543
5	0.017985350853863987
6	0.023094813489676323
7	0.008416894182361393
8	0.0042767780760469415
9	0.0035140135938190992
10-14	0.003857469489844469
15-19	0.005503769497319561
20-24	0.010458348567435076
25-29	0.021495762504294788
30-34	0.028882924635648594
35-39	0.03000906164204887
40-44	0.029272782037676158
45-49	0.035953115748832164
50-54	0.031830585601413525
55-59	0.03620991312451554
60-64	0.032895277691189884
65-69	0.02524241926519338
70-74	0.02415484424095018
75-79	0.026815409130765463
80-84	0.03993432258801582
85-89	0.04205798603395183
90-94	0.05349945326736946
95-99	0.04968711400939014
100-104	0.051231712085901514
105-109	0.06022661224257335
110-114	0.06101670911874768
115-119	0.07136318556212266
120-124	0.0634272567616105
125-129	0.07355888787582465
130-134	0.062372099227861987
135-139	0.057843820751702704
140-144	0.06904819535856117
145-149	0.06753792168375004
150	0.08088587636469159
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	9.4393488E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	89.2418509262326
#Duplication Level	Relative count
1	100.0
2	19.392959323298918
3	11.397561885806692
4	8.611145664883692
5	6.854086329145416
6	5.874486876477174
7	5.258738649085707
8	4.770493842517726
9	4.095658664012937
10++	455.7127752207986
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	20270660	3.5762684	4.259962	6
>>END_MODULE