##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	N0062.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	67904072	
Filtered Sequences	0	
Sequence length	150	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.400590011744804	32.0	32.0	32.0	27.0	32.0
2	30.99762062870103	32.0	32.0	32.0	32.0	32.0
3	30.94812774998236	32.0	32.0	32.0	27.0	32.0
4	30.96622470593516	32.0	32.0	32.0	27.0	32.0
5	30.928917620728253	32.0	32.0	32.0	27.0	32.0
6	35.361402229309604	37.0	37.0	37.0	32.0	37.0
7	35.2342135093165	37.0	37.0	37.0	32.0	37.0
8	35.20650548026045	37.0	37.0	37.0	32.0	37.0
9	35.20396692852234	37.0	37.0	37.0	32.0	37.0
10-14	35.11083168915113	37.0	37.0	37.0	32.0	37.0
15-19	35.02812141221811	37.0	37.0	37.0	32.0	37.0
20-24	34.984093186635405	37.0	37.0	37.0	32.0	37.0
25-29	34.974567937545785	37.0	37.0	37.0	32.0	37.0
30-34	34.92687901838936	37.0	37.0	37.0	32.0	37.0
35-39	34.860791090702186	37.0	37.0	37.0	32.0	37.0
40-44	34.797439084360064	37.0	37.0	37.0	30.0	37.0
45-49	34.72825823464608	37.0	37.0	37.0	27.0	37.0
50-54	34.66476831610335	37.0	37.0	37.0	27.0	37.0
55-59	34.59902451210879	37.0	37.0	37.0	27.0	37.0
60-64	34.52722169297888	37.0	37.0	37.0	27.0	37.0
65-69	34.449135969342166	37.0	37.0	37.0	27.0	37.0
70-74	34.36517203563286	37.0	37.0	37.0	27.0	37.0
75-79	34.299470791678004	37.0	37.0	37.0	27.0	37.0
80-84	34.20809193592985	37.0	37.0	37.0	27.0	37.0
85-89	34.09914181877046	37.0	37.0	37.0	27.0	37.0
90-94	33.96570105250831	37.0	37.0	37.0	27.0	37.0
95-99	33.823698796737844	37.0	37.0	37.0	27.0	37.0
100-104	33.68081704437401	37.0	34.0	37.0	24.0	37.0
105-109	33.53102545013795	37.0	32.0	37.0	22.0	37.0
110-114	33.394527538790314	37.0	32.0	37.0	22.0	37.0
115-119	33.253191207737885	37.0	32.0	37.0	22.0	37.0
120-124	33.14956414984951	37.0	32.0	37.0	22.0	37.0
125-129	33.02195289260415	37.0	32.0	37.0	22.0	37.0
130-134	32.929942566625456	37.0	32.0	37.0	22.0	37.0
135-139	32.846769784292164	37.0	32.0	37.0	22.0	37.0
140-144	32.72310572479365	37.0	32.0	37.0	22.0	37.0
145-149	32.48427836846073	37.0	32.0	37.0	20.2	37.0
150	30.765743930643804	37.0	27.0	37.0	13.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	58.0
5	975.0
6	1747.0
7	1496.0
8	1343.0
9	1344.0
10	1403.0
11	4052.0
12	9858.0
13	14246.0
14	17588.0
15	23825.0
16	30646.0
17	36640.0
18	45399.0
19	64922.0
20	102403.0
21	154993.0
22	220836.0
23	305462.0
24	406357.0
25	526415.0
26	672549.0
27	858067.0
28	1096691.0
29	1426271.0
30	1911792.0
31	2693437.0
32	4098961.0
33	6935754.0
34	1.3215553E7
35	2.3783453E7
36	9239534.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.79620963900245	32.01809523580723	22.41789684107892	27.767798284111393
2	23.97454817700597	31.935009362289236	25.461371538829336	18.629070921875456
3	20.266103960389405	29.882574569806142	26.713210198460956	23.1381112713435
4	21.538751007892365	27.130573281856634	28.336476347450656	22.994199362800344
5	23.51456561706355	27.99536169503693	27.999231073367465	20.49084161453206
6	22.24762223099348	28.18695404171091	26.919450837777536	22.645972889518077
7	24.07004141481979	27.938770280301156	27.349369057297373	20.64181924758168
8	22.02722609613636	27.79406124588884	28.241004890960802	21.937707767013997
9	21.945741434041608	26.7910704541584	29.06061357771333	22.202574534086665
10-14	22.600981597532947	27.94253366545631	27.28422435178818	22.172260385222568
15-19	22.444336600292438	27.86992934892392	27.312176655318876	22.37355739546476
20-24	22.635812870064566	27.741472627661413	27.211242092118308	22.41147241015571
25-29	22.670150440902106	27.734365606321987	27.178859001051613	22.416624951724298
30-34	22.738879143668154	27.586755087028646	27.110330809941217	22.564034959361983
35-39	22.763892654298942	27.64788505993253	27.052596072559304	22.535626213209227
40-44	22.791916978856356	27.62504474399488	27.044489191606463	22.538549085542297
45-49	22.795321753593228	27.5872344896119	27.030290277689435	22.58715347910544
50-54	22.7807314717936	27.648049409277135	27.00973454395187	22.56148457497739
55-59	22.802344339206673	27.61975033035534	27.010486073219454	22.567419257218532
60-64	22.79120770781782	27.593682403114013	26.988985459033206	22.626124430034963
65-69	22.80738402441963	27.647211844050123	26.968726716025348	22.576677415504896
70-74	22.81100264412743	27.649664724090105	26.997415435484417	22.54191719629804
75-79	22.759432102533758	27.657652615567102	26.978121713313207	22.604793568585933
80-84	22.77914984425414	27.67718633886784	26.953429293573173	22.590234523304847
85-89	22.77360650722155	27.70391090731946	26.95837095959935	22.564111625859635
90-94	22.759947269998776	27.699264266793357	26.94674653323947	22.5940419299684
95-99	22.740095830673507	27.72741957009538	26.976577111136272	22.555907488094835
100-104	22.776075159703513	27.72914289699388	26.937781442971986	22.557000500330616
105-109	22.776481599745306	27.663363330736708	26.945817847230106	22.61433722228788
110-114	22.80192097255924	27.692832497878268	26.923290451646036	22.581956077916455
115-119	22.784600674592216	27.739566382248892	26.91775386083326	22.55807908232563
120-124	22.78463158407234	27.70201711940003	26.941968936716076	22.571382359811548
125-129	22.77944321224076	27.708187378182032	26.923406097682356	22.588963311894855
130-134	22.783600802176654	27.738192670319368	26.909808634624067	22.56839789287991
135-139	22.75347403685337	27.735295248518497	26.908444625686823	22.60278608894131
140-144	22.80441410350913	27.76719174740927	26.89261107832401	22.535783070757596
145-149	22.84944223212451	27.776595751327925	26.85491112645876	22.519050890088806
150	22.90908609097239	27.23249397331889	26.82178474182471	23.03663519388401
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	45.56400792311385
2	42.60361909888143
3	43.4042152317329
4	44.53295037069271
5	44.005407231595605
6	44.893595120511556
7	44.71186066240147
8	43.96493386315036
9	44.14831596812827
10-14	44.773241982755515
15-19	44.8178939957572
20-24	45.04728528022028
25-29	45.0867753926264
30-34	45.30291410303014
35-39	45.29951886750817
40-44	45.33046606439866
45-49	45.38247523269867
50-54	45.342216046770986
55-59	45.36976359642521
60-64	45.41733213785278
65-69	45.38406143992453
70-74	45.35291984042547
75-79	45.36422567111969
80-84	45.369384367558986
85-89	45.33771813308119
90-94	45.353989199967174
95-99	45.29600331876834
100-104	45.33307566003413
105-109	45.39081882203318
110-114	45.3838770504757
115-119	45.342679756917846
120-124	45.35601394388389
125-129	45.368406524135615
130-134	45.351998695056565
135-139	45.35626012579468
140-144	45.34019717426672
145-149	45.368493122213316
150	45.9457212848564
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	256.0
1	303.5
2	458.0
3	672.0
4	853.5
5	947.5
6	1051.0
7	1152.0
8	1281.5
9	1429.0
10	1607.5
11	1859.5
12	2093.0
13	2327.0
14	2684.0
15	4503.5
16	7725.5
17	11379.0
18	15823.0
19	16758.5
20	17130.0
21	22522.0
22	33688.0
23	46722.0
24	60418.0
25	75526.5
26	99350.0
27	135630.5
28	172505.0
29	203626.0
30	242603.0
31	313798.5
32	394508.5
33	505120.5
34	678226.0
35	874696.5
36	1108411.0
37	1396738.0
38	1707025.0
39	2003992.0
40	2401604.5
41	2893063.0
42	3339387.5
43	3812269.5
44	4257549.0
45	4609050.0
46	4818672.0
47	4807944.0
48	4608187.0
49	4249839.0
50	3800425.5
51	3298176.5
52	2766296.0
53	2253050.5
54	1739256.0
55	1261144.5
56	902692.5
57	644471.0
58	450028.0
59	316769.5
60	196748.5
61	99449.5
62	59285.5
63	35397.0
64	17879.0
65	7605.5
66	3626.5
67	2200.0
68	1727.0
69	1487.5
70	1337.0
71	1224.5
72	1116.5
73	1053.0
74	998.0
75	948.5
76	915.0
77	915.5
78	897.0
79	855.0
80	868.0
81	867.0
82	833.5
83	824.5
84	828.5
85	825.0
86	850.0
87	896.0
88	924.0
89	1017.0
90	1111.5
91	1306.0
92	1705.5
93	2258.0
94	3077.0
95	7212.0
96	11634.0
97	9420.5
98	4426.5
99	2360.0
100	9580.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08172558488097739
2	0.05938966370087497
3	0.04583672095540898
4	0.027390109977498845
5	0.017714696108357096
6	0.02303838273498532
7	0.00835296004045236
8	0.004261894632769593
9	0.0035167257716149924
10-14	0.0037650172142842924
15-19	0.005331049955295759
20-24	0.010311016399723422
25-29	0.02110359449430367
30-34	0.028402125869564934
35-39	0.029553455940020797
40-44	0.028780895496222966
45-49	0.03532601108222199
50-54	0.03128118737857134
55-59	0.03554131481246073
60-64	0.03235358256571123
65-69	0.025010871218444752
70-74	0.023848643421560935
75-79	0.026342750107828584
80-84	0.03912931760557747
85-89	0.04116189085096399
90-94	0.052443983035362
95-99	0.048759962436420605
100-104	0.05029182933241471
105-109	0.059067444438383604
110-114	0.05993396095597919
115-119	0.07020344818201771
120-124	0.06236444848255933
125-129	0.07221746583916204
130-134	0.06113565619452099
135-139	0.056930017392771384
140-144	0.06787398552475617
145-149	0.06640308699012926
150	0.07970656016033914
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	6.7904072E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	88.86847643176864
#Duplication Level	Relative count
1	100.0
2	19.672630119381786
3	11.57293111028442
4	8.71520849538368
5	7.125705975853089
6	6.3038993540532315
7	5.45165544996449
8	5.062734620717643
9	4.812472521897933
10++	507.66681321654437
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE