##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	N0063.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	76979880	
Filtered Sequences	0	
Sequence length	150	
%GC	48	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.792480164946998	32.0	27.0	32.0	27.0	32.0
2	30.100315472042826	32.0	32.0	32.0	27.0	32.0
3	30.023602699822344	32.0	32.0	32.0	27.0	32.0
4	30.01518638376677	32.0	32.0	32.0	27.0	32.0
5	30.168819008291518	32.0	32.0	32.0	27.0	32.0
6	34.71956872367169	37.0	37.0	37.0	27.0	37.0
7	34.82675727735611	37.0	37.0	37.0	32.0	37.0
8	34.823417729411894	37.0	37.0	37.0	32.0	37.0
9	34.80765427277881	37.0	37.0	37.0	27.0	37.0
10-14	34.724289390942154	37.0	37.0	37.0	27.0	37.0
15-19	34.669207987853454	37.0	37.0	37.0	27.0	37.0
20-24	34.63071759010277	37.0	37.0	37.0	27.0	37.0
25-29	34.61291618537207	37.0	37.0	37.0	27.0	37.0
30-34	34.56838244486741	37.0	37.0	37.0	27.0	37.0
35-39	34.50004783067992	37.0	37.0	37.0	27.0	37.0
40-44	34.42996186016398	37.0	37.0	37.0	27.0	37.0
45-49	34.35723890969952	37.0	37.0	37.0	27.0	37.0
50-54	34.29645789003568	37.0	37.0	37.0	27.0	37.0
55-59	34.225621689719446	37.0	37.0	37.0	27.0	37.0
60-64	34.1521881483837	37.0	37.0	37.0	27.0	37.0
65-69	34.06707323264209	37.0	37.0	37.0	27.0	37.0
70-74	33.979464122313516	37.0	37.0	37.0	26.0	37.0
75-79	33.91366632423953	37.0	37.0	37.0	22.0	37.0
80-84	33.82096921429339	37.0	37.0	37.0	22.0	37.0
85-89	33.71097432471966	37.0	37.0	37.0	22.0	37.0
90-94	33.57535404575845	37.0	37.0	37.0	22.0	37.0
95-99	33.42996772403386	37.0	32.0	37.0	22.0	37.0
100-104	33.28545550343804	37.0	32.0	37.0	22.0	37.0
105-109	33.13050005014297	37.0	32.0	37.0	22.0	37.0
110-114	32.990769806344204	37.0	32.0	37.0	22.0	37.0
115-119	32.844923434539	37.0	32.0	37.0	22.0	37.0
120-124	32.73686797381342	37.0	32.0	37.0	22.0	37.0
125-129	32.60916079110542	37.0	32.0	37.0	18.4	37.0
130-134	32.509770594602124	37.0	32.0	37.0	13.0	37.0
135-139	32.4251870670622	37.0	32.0	37.0	13.0	37.0
140-144	32.29508094582637	37.0	32.0	37.0	13.0	37.0
145-149	32.05388196759984	37.0	32.0	37.0	13.0	37.0
150	30.278467087763712	37.0	27.0	37.0	13.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	95.0
5	1120.0
6	1760.0
7	1490.0
8	1343.0
9	1339.0
10	1641.0
11	6564.0
12	16303.0
13	22319.0
14	25518.0
15	30756.0
16	38894.0
17	49106.0
18	65981.0
19	103016.0
20	170838.0
21	268600.0
22	392906.0
23	542686.0
24	720722.0
25	923503.0
26	1152811.0
27	1410978.0
28	1718587.0
29	2105382.0
30	2641512.0
31	3450434.0
32	4854946.0
33	7678326.0
34	1.4078189E7
35	2.4859014E7
36	9643197.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.04964597128391	30.666252786224575	20.296298738341513	29.987802504150007
2	26.48559513956187	30.055757940947714	23.939364360253045	19.519282559237368
3	22.112050222954498	28.22617468424651	24.52745507364522	25.134320019153773
4	22.86676579112217	26.759655506351375	25.021706592564573	25.351872109961874
5	24.670105613600363	27.778633696153797	25.171438906050565	22.379821784195276
6	23.626279140543797	27.00744113240206	25.473361860292325	23.892917866761817
7	25.48411525256408	26.86020169103877	25.870347614452182	21.78533544194497
8	22.994147601304306	26.777759687193075	26.911002407958872	23.317090303543743
9	23.296540734250037	25.77705620330095	27.411194101038582	23.515208961410426
10-14	23.98802395828786	26.754371512002418	25.633019835727	23.624584693982722
15-19	23.93579632164879	26.599778743471127	25.7006487493183	23.763776185561785
20-24	24.104827327061955	26.46407869898307	25.61438980177305	23.816704172181925
25-29	24.095402662508274	26.45501926149963	25.589571076875284	23.860006999116816
30-34	24.15835409851157	26.35731963411263	25.508250939804622	23.976075327571174
35-39	24.228360308095738	26.36457926545829	25.450850557994535	23.956209868451435
40-44	24.26373868885045	26.38348034193294	25.390190521372745	23.962590447843866
45-49	24.24944196129814	26.312101359066364	25.40463361036686	24.033823069268635
50-54	24.254534326862487	26.335075796583546	25.38543430842998	24.02495556812399
55-59	24.261473514279494	26.35772506723108	25.355909398414806	24.02489202007462
60-64	24.23137112274651	26.34252061956734	25.36464295352913	24.061465304157018
65-69	24.238015317903166	26.378240714970612	25.331305280499492	24.052438686626726
70-74	24.267486495485453	26.391570008314343	25.331654203318703	24.009289292881505
75-79	24.221833807242767	26.38342015758673	25.325117238293878	24.069628796876636
80-84	24.23485422339453	26.395619692658627	25.360014239115607	24.009511844831234
85-89	24.244685712826076	26.424072508728564	25.298823512851076	24.032418265594288
90-94	24.243571011853202	26.39276100438431	25.305460913791567	24.058207069970916
95-99	24.181492713957518	26.427341841709385	25.32440635876258	24.06675908557052
100-104	24.229569657322596	26.456917841636614	25.285734842758163	24.027777658282627
105-109	24.19014239763422	26.41399099178813	25.312264164740395	24.08360244583726
110-114	24.2101502161246	26.42219256086041	25.31850485451526	24.049152368499726
115-119	24.24474017863128	26.467421844941708	25.24939371648121	24.038444259945805
120-124	24.177629611724033	26.456319501184666	25.293820389952316	24.072230497138985
125-129	24.213047064176006	26.45019231949355	25.286545744849377	24.050214871481074
130-134	24.22498196423376	26.508033896634313	25.230098995508165	24.036885143623763
135-139	24.22401488649172	26.459723006086534	25.234399473014435	24.08186263440732
140-144	24.23300468011967	26.493246312723638	25.236605769867726	24.03714323728896
145-149	24.246169306830527	26.537910001646225	25.214373526389007	24.001547165134244
150	24.26907191255976	25.911345449523175	25.14102069567142	24.678561942245643
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	49.03744847543391
2	46.00487769879924
3	47.24637024210827
4	48.21863790108405
5	47.049927397795635
6	47.519197007305614
7	47.269450694509054
8	46.31123790484805
9	46.81174969566047
10-14	47.61260865227058
15-19	47.699572507210576
20-24	47.92153149924388
25-29	47.95540966162509
30-34	48.13442942608275
35-39	48.184570176547176
40-44	48.22632913669432
45-49	48.28326503056677
50-54	48.27948989498647
55-59	48.28636553435411
60-64	48.29283642690353
65-69	48.29045400452989
70-74	48.27677578836696
75-79	48.2914626041194
80-84	48.244366068225766
85-89	48.277103978420364
90-94	48.30177808182412
95-99	48.248251799528035
100-104	48.25734731560522
105-109	48.273744843471476
110-114	48.25930258462433
115-119	48.28318443857708
120-124	48.24986010886302
125-129	48.26326193565708
130-134	48.26186710785752
135-139	48.30587752089904
140-144	48.270147917408636
145-149	48.24771647196477
150	48.9476338548054
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	117.0
1	198.0
2	410.0
3	680.0
4	941.5
5	1144.0
6	1312.5
7	1441.0
8	1525.5
9	1690.0
10	1935.5
11	2247.0
12	3444.5
13	7226.0
14	9970.0
15	9577.0
16	10016.0
17	12171.5
18	16347.0
19	25366.5
20	42399.0
21	65335.0
22	87874.5
23	126975.0
24	172967.0
25	211468.5
26	266853.0
27	334670.0
28	406799.5
29	452652.0
30	483914.0
31	572320.0
32	664489.0
33	762004.5
34	894264.5
35	1024998.0
36	1115355.0
37	1159381.0
38	1250964.0
39	1394081.5
40	1563584.0
41	1750885.5
42	1897367.0
43	2030777.0
44	2271131.5
45	2522958.0
46	2768132.5
47	3088402.0
48	3429170.0
49	3763775.5
50	4084565.0
51	4342847.0
52	4447638.0
53	4392388.0
54	4215191.5
55	3847807.5
56	3308965.0
57	2719463.0
58	2191379.5
59	1746939.0
60	1350851.0
61	1038278.0
62	769563.5
63	541524.5
64	380159.0
65	264553.5
66	182801.0
67	132341.0
68	93807.0
69	68012.0
70	46668.0
71	24714.5
72	13570.5
73	7367.0
74	3378.5
75	1998.0
76	1684.5
77	1532.0
78	1442.0
79	1384.5
80	1370.5
81	1309.0
82	1273.0
83	1251.5
84	1225.5
85	1290.5
86	1284.0
87	1256.5
88	1261.5
89	1290.0
90	1389.5
91	1581.0
92	1866.0
93	2209.5
94	2711.0
95	4822.0
96	6655.5
97	5540.0
98	3654.5
99	3204.0
100	14359.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07260858291803
2	0.054507749297608675
3	0.041721291329630546
4	0.02518839987799409
5	0.016263990019210212
6	0.021032768562382793
7	0.007805935784779087
8	0.00398415793841196
9	0.0032904701851964435
10-14	0.003541964471755477
15-19	0.0050553469296132966
20-24	0.009584842169148615
25-29	0.01914889968651549
30-34	0.025498610805836538
35-39	0.026196455489408403
40-44	0.02551705718429283
45-49	0.03154798370691147
50-54	0.027642807445270118
55-59	0.03160020514451308
60-64	0.029126311966191688
65-69	0.02258772032380409
70-74	0.02168280854685666
75-79	0.024097465467600103
80-84	0.03625596714362246
85-89	0.03820374882371862
90-94	0.048734032840789046
95-99	0.04529962894200407
100-104	0.04650383970460853
105-109	0.0547795086196549
110-114	0.05563349799973707
115-119	0.06520560956966938
120-124	0.05806530225820045
125-129	0.06735370333131202
130-134	0.05698112285963553
135-139	0.05266726838233575
140-144	0.0630770014190721
145-149	0.06161713943955225
150	0.07421159918669658
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	7.697988E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	85.24919077652083
#Duplication Level	Relative count
1	100.0
2	19.328111302774087
3	12.16808528688177
4	9.13949608347708
5	7.751039221785482
6	6.99035715295648
7	6.328648587506716
8	6.0713174787206965
9	5.969516160959195
10++	391.8163051777281
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE