##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	N0064.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	99534888	
Filtered Sequences	0	
Sequence length	150	
%GC	46	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.374319504935798	32.0	32.0	32.0	27.0	32.0
2	30.936407573995563	32.0	32.0	32.0	27.0	32.0
3	30.858223450253945	32.0	32.0	32.0	27.0	32.0
4	30.71102459069427	32.0	32.0	32.0	27.0	32.0
5	30.670783142891565	32.0	32.0	32.0	27.0	32.0
6	35.05781863139284	37.0	37.0	37.0	32.0	37.0
7	35.10074729777161	37.0	37.0	37.0	32.0	37.0
8	35.067442995465065	37.0	37.0	37.0	32.0	37.0
9	35.07527003998839	37.0	37.0	37.0	32.0	37.0
10-14	34.99599451601332	37.0	37.0	37.0	32.0	37.0
15-19	34.92562559973946	37.0	37.0	37.0	32.0	37.0
20-24	34.87886625441323	37.0	37.0	37.0	32.0	37.0
25-29	34.871368802866385	37.0	37.0	37.0	32.0	37.0
30-34	34.82289642401567	37.0	37.0	37.0	32.0	37.0
35-39	34.75622764351732	37.0	37.0	37.0	29.0	37.0
40-44	34.68743091568054	37.0	37.0	37.0	27.0	37.0
45-49	34.614451150032934	37.0	37.0	37.0	27.0	37.0
50-54	34.55055597591068	37.0	37.0	37.0	27.0	37.0
55-59	34.480426603785396	37.0	37.0	37.0	27.0	37.0
60-64	34.410333524462295	37.0	37.0	37.0	27.0	37.0
65-69	34.32491515537747	37.0	37.0	37.0	27.0	37.0
70-74	34.23999937187854	37.0	37.0	37.0	27.0	37.0
75-79	34.17430503362801	37.0	37.0	37.0	27.0	37.0
80-84	34.08254632285315	37.0	37.0	37.0	27.0	37.0
85-89	33.971559598278745	37.0	37.0	37.0	27.0	37.0
90-94	33.8330845682973	37.0	37.0	37.0	26.0	37.0
95-99	33.69047771671778	37.0	36.0	37.0	22.0	37.0
100-104	33.54430961935678	37.0	32.0	37.0	22.0	37.0
105-109	33.39035096116248	37.0	32.0	37.0	22.0	37.0
110-114	33.24830820525965	37.0	32.0	37.0	22.0	37.0
115-119	33.107601891308704	37.0	32.0	37.0	22.0	37.0
120-124	32.99805312485005	37.0	32.0	37.0	22.0	37.0
125-129	32.866319335186276	37.0	32.0	37.0	22.0	37.0
130-134	32.76895437105429	37.0	32.0	37.0	22.0	37.0
135-139	32.682879170969684	37.0	32.0	37.0	22.0	37.0
140-144	32.557053221379014	37.0	32.0	37.0	22.0	37.0
145-149	32.31503179066219	37.0	32.0	37.0	13.0	37.0
150	30.513382302695714	37.0	27.0	37.0	13.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	124.0
5	1440.0
6	2731.0
7	2109.0
8	1931.0
9	1924.0
10	2010.0
11	6422.0
12	17034.0
13	24042.0
14	28198.0
15	35511.0
16	45148.0
17	55716.0
18	72344.0
19	110523.0
20	177579.0
21	270014.0
22	386153.0
23	522914.0
24	685688.0
25	875064.0
26	1102674.0
27	1384758.0
28	1749691.0
29	2246042.0
30	2974673.0
31	4134222.0
32	6207215.0
33	1.0387677E7
34	1.9568696E7
35	3.4000281E7
36	1.2454336E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.841243255268523	30.68345355389061	22.209738556423222	28.265564634417643
2	25.771603037860196	30.397036791808567	25.217141488182843	18.61421868214839
3	21.544319380472484	28.78495598816273	26.180098150476987	23.490626480887805
4	22.201949618919407	26.825608593145112	27.507815628233566	23.46462615970191
5	24.262445177815213	27.451639341313623	27.316446003284394	20.969469477586774
6	23.096513611081967	27.215287349530044	26.287583070750774	23.40061596863721
7	24.842616237280538	27.099031674127495	26.681511920631962	21.37684016796001
8	22.606833230738367	27.095488311789463	27.50683237471977	22.790846082752402
9	22.72557417612065	26.123923506272913	28.195983193275453	22.954519124330986
10-14	23.38668058833516	27.172321332411492	26.45280446736234	22.988193611891006
15-19	23.305941274199558	27.075559917528107	26.460407891133723	23.158090917138612
20-24	23.43898095397371	26.9569357773262	26.353395786033317	23.250687482666763
25-29	23.480394480724755	26.943063189128303	26.29365716109312	23.282885169053824
30-34	23.5876454381057	26.84680099791892	26.18978281141314	23.375770752562243
35-39	23.620304793042813	26.866636146591144	26.15175690588799	23.361302154478054
40-44	23.635694400697624	26.866648551781676	26.13549727620481	23.36215977131589
45-49	23.64956001715812	26.801366651499887	26.12728601807565	23.421787313266346
50-54	23.609432193599368	26.864927305623187	26.09396608838187	23.431674412395576
55-59	23.628723956832584	26.859750201554917	26.092890157279868	23.41863568433263
60-64	23.616948876197277	26.845877605530443	26.09240452806484	23.444768990207443
65-69	23.63219450816475	26.893312974377125	26.08595806900688	23.388534448451246
70-74	23.638092652912444	26.876985958668897	26.063553583624998	23.421367804793658
75-79	23.582590290477913	26.88921340242904	26.078868704861673	23.449327602231374
80-84	23.577605771961423	26.935857080553184	26.067540694687004	23.4189964527984
85-89	23.61665455646233	26.92421069176476	26.07557022800014	23.38356452377277
90-94	23.5664021061346	26.930206306230698	26.066609657118335	23.436781930516364
95-99	23.606279918377798	26.923301702910884	26.06497198540189	23.40544639330943
100-104	23.613827892562895	26.98303280299611	26.02115048775352	23.38198881668748
105-109	23.597634545767885	26.90507536572271	26.06389402235154	23.43339606615787
110-114	23.569158178616497	26.971038606553165	26.05271415479119	23.407089060039144
115-119	23.63004133994037	26.958198354238398	26.023678208584283	23.388082097236946
120-124	23.61357131261447	26.934012602856477	26.046947503925637	23.405468580603415
125-129	23.559132563021326	26.913861981935312	26.0960425314149	23.430962923628464
130-134	23.619321150448243	26.971474355923657	26.039311310002212	23.369893183625887
135-139	23.591045121249092	26.97254961710012	26.005188312174372	23.43121694947641
140-144	23.624929480059762	26.963270370741405	26.00514362502012	23.40665652417871
145-149	23.658877307072927	26.959530815962125	26.001437207268964	23.380154669695987
150	23.71940850486911	26.269937352919847	25.968038820170992	24.04261532204005
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	47.106807889686166
2	44.385821720008586
3	45.034945861360285
4	45.66657577862132
5	45.23191465540199
6	46.49712957971918
7	46.21945640524055
8	45.39767931349077
9	45.68009330045164
10-14	46.37487420022616
15-19	46.46403219133817
20-24	46.689668436640474
25-29	46.763279649778575
30-34	46.96341619066794
35-39	46.981606947520866
40-44	46.99785417201351
45-49	47.07134733042446
50-54	47.04110660599494
55-59	47.047359641165215
60-64	47.06171786640472
65-69	47.020728956616
70-74	47.0594604577061
75-79	47.03191789270929
80-84	46.99660222475982
85-89	47.0002190802351
90-94	47.003184036650964
95-99	47.01172631168723
100-104	46.99581670925037
105-109	47.03103061192575
110-114	46.97624723865564
115-119	47.018123437177316
120-124	47.01903989321789
125-129	46.99009548664979
130-134	46.989214334074134
135-139	47.0222620707255
140-144	47.03158600423848
145-149	47.03903197676892
150	47.762023826909164
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	138.0
1	252.0
2	482.5
3	734.5
4	935.5
5	1117.0
6	1299.0
7	1459.0
8	1681.0
9	1946.0
10	2214.0
11	2568.0
12	3040.5
13	3475.0
14	4093.5
15	5367.5
16	9838.5
17	13967.0
18	13475.5
19	16611.0
20	28532.0
21	84001.5
22	142631.5
23	179513.5
24	223685.5
25	274456.5
26	353831.0
27	427861.5
28	497883.0
29	635244.0
30	821514.5
31	1003719.0
32	1202916.5
33	1306594.5
34	1383003.0
35	1571559.5
36	1793878.0
37	1963103.0
38	2052507.0
39	2219615.0
40	2491257.0
41	2751587.5
42	3016178.0
43	3351889.5
44	3667299.0
45	3917552.5
46	4157158.0
47	4465447.5
48	4833277.0
49	5022850.5
50	4950774.0
51	4817509.0
52	4768661.5
53	4674060.5
54	4403614.0
55	4010085.0
56	3531733.0
57	2964298.0
58	2415319.5
59	1947699.0
60	1545135.5
61	1167551.5
62	821537.5
63	566792.0
64	370938.0
65	221500.0
66	129928.5
67	75717.0
68	43377.0
69	25892.0
70	14561.0
71	7135.5
72	4133.0
73	2845.0
74	2126.5
75	1869.5
76	1905.5
77	1870.5
78	1671.5
79	1506.0
80	1417.5
81	1385.0
82	1383.5
83	1407.0
84	1449.5
85	1454.5
86	1493.5
87	1526.5
88	1599.5
89	1786.0
90	2027.0
91	2373.5
92	3016.5
93	3950.5
94	5202.5
95	12640.5
96	20079.5
97	15512.0
98	7013.5
99	3709.0
100	14665.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08264740298899015
2	0.060281375913137106
3	0.04664294192002306
4	0.027982148329739415
5	0.01823481230018564
6	0.02343700833822207
7	0.00861908841450648
8	0.004360280186380478
9	0.0035535278846146887
10-14	0.003924453102313231
15-19	0.005631392281267248
20-24	0.010829167758746058
25-29	0.02180783083816802
30-34	0.02922432584643085
35-39	0.030250900568652872
40-44	0.029378643596805976
45-49	0.03603922274971566
50-54	0.0317685593819124
55-59	0.03611798910146963
60-64	0.03282587709346697
65-69	0.025385872740420423
70-74	0.02430725596436096
75-79	0.026889868002865486
80-84	0.03991103099447904
85-89	0.04185366642498257
90-94	0.053556095828429524
95-99	0.04973713337578679
100-104	0.0512931706920693
105-109	0.06018472638458185
110-114	0.06107024503810162
115-119	0.07136090814710115
120-124	0.06354374960466123
125-129	0.07376689869787165
130-134	0.062451670212358104
135-139	0.05795133863012937
140-144	0.06901580077128333
145-149	0.06757288961836176
150	0.08098868810702836
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	9.9534888E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	88.89573347427509
#Duplication Level	Relative count
1	100.0
2	19.49514180672269
3	11.722032563025211
4	9.315913865546218
5	7.9963235294117645
6	6.676733193277311
7	6.102284663865547
8	5.600052521008403
9	5.734637605042017
10++	483.8694852941176
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE