##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	N0066.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	111341086	
Filtered Sequences	0	
Sequence length	150	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.190534848923605	32.0	32.0	32.0	27.0	32.0
2	30.843792910372727	32.0	32.0	32.0	27.0	32.0
3	30.561616661436194	32.0	32.0	32.0	27.0	32.0
4	30.55879315745133	32.0	32.0	32.0	27.0	32.0
5	30.45083340573847	32.0	32.0	32.0	27.0	32.0
6	34.7468399401098	37.0	37.0	37.0	27.0	37.0
7	34.80245746839581	37.0	37.0	37.0	32.0	37.0
8	34.81678868302039	37.0	37.0	37.0	32.0	37.0
9	34.7972992826745	37.0	37.0	37.0	27.0	37.0
10-14	34.76416145608639	37.0	37.0	37.0	27.0	37.0
15-19	34.71111320577563	37.0	37.0	37.0	27.0	37.0
20-24	34.67351069667131	37.0	37.0	37.0	27.0	37.0
25-29	34.702582103429464	37.0	37.0	37.0	27.0	37.0
30-34	34.67815840596346	37.0	37.0	37.0	27.0	37.0
35-39	34.62303931183139	37.0	37.0	37.0	27.0	37.0
40-44	34.57536025470419	37.0	37.0	37.0	27.0	37.0
45-49	34.51514922712358	37.0	37.0	37.0	27.0	37.0
50-54	34.452589569676014	37.0	37.0	37.0	27.0	37.0
55-59	34.410774613784525	37.0	37.0	37.0	27.0	37.0
60-64	34.35221319468718	37.0	37.0	37.0	27.0	37.0
65-69	34.27917887382561	37.0	37.0	37.0	27.0	37.0
70-74	34.21700121193357	37.0	37.0	37.0	27.0	37.0
75-79	34.149299167065784	37.0	37.0	37.0	27.0	37.0
80-84	34.08556022347402	37.0	37.0	37.0	27.0	37.0
85-89	34.02322253440208	37.0	37.0	37.0	27.0	37.0
90-94	33.932826996136896	37.0	37.0	37.0	27.0	37.0
95-99	33.8208195292796	37.0	37.0	37.0	24.0	37.0
100-104	33.6871562057514	37.0	37.0	37.0	22.0	37.0
105-109	33.54497015055161	37.0	32.0	37.0	22.0	37.0
110-114	33.402101707540375	37.0	32.0	37.0	22.0	37.0
115-119	33.241401300863906	37.0	32.0	37.0	22.0	37.0
120-124	33.114692369715165	37.0	32.0	37.0	22.0	37.0
125-129	32.97886671592192	37.0	32.0	37.0	22.0	37.0
130-134	32.84330682745451	37.0	32.0	37.0	22.0	37.0
135-139	32.74639442442658	37.0	32.0	37.0	22.0	37.0
140-144	32.65129437842918	37.0	32.0	37.0	22.0	37.0
145-149	32.52823054914337	37.0	32.0	37.0	22.0	37.0
150	31.100408873324625	37.0	27.0	37.0	13.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	263.0
11	3211.0
12	12437.0
13	21403.0
14	26401.0
15	30581.0
16	34674.0
17	41346.0
18	58050.0
19	100312.0
20	181873.0
21	304499.0
22	467510.0
23	672106.0
24	914789.0
25	1192658.0
26	1501152.0
27	1857716.0
28	2287821.0
29	2831548.0
30	3568794.0
31	4648027.0
32	6447509.0
33	9939125.0
34	1.8409394E7
35	3.8350028E7
36	1.7437856E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.655939090981082	32.56239674158161	23.409988147131685	26.371676020305618
2	23.227107278842457	31.623415536833328	26.18549144085998	18.96398574346423
3	21.28493824641841	30.489065004479716	26.14886620578158	22.077130543320298
4	21.372228287497794	28.426316772683517	27.36926068802095	22.832194251797734
5	22.992881424571916	28.8160976975158	27.164908118028503	21.026112759883777
6	22.4854386572585	28.940010563577296	26.62294456020816	21.95160621895604
7	23.48777842758791	28.609645670254903	27.22632967791557	20.676246224241616
8	21.564191578308762	28.60957203925953	28.410677498120773	21.415558884310936
9	21.42292762472529	27.80820697071606	29.06963029747564	21.699235107083016
10-14	22.16957950518989	28.6338263764191	27.48468023058906	21.71191388780195
15-19	22.10953730415623	28.530290544218435	27.44696536452124	21.913206787104087
20-24	22.20057943835839	28.379690333016512	27.44334111800662	21.976389110618477
25-29	22.29022108603825	28.335698960163384	27.35111638094147	22.02296357285689
30-34	22.31087396118786	28.26185993227665	27.32332724872944	22.103938857806053
35-39	22.35983461580353	28.28522405167427	27.288270988862873	22.066670343659332
40-44	22.43002104583997	28.250402278840298	27.242212611427608	22.077364063892126
45-49	22.41111733622798	28.216718791791635	27.22317211637827	22.14899175560211
50-54	22.40303660642402	28.246410836875626	27.222076066723705	22.128476489976652
55-59	22.393663377775926	28.241909729531468	27.19360524290198	22.170821649790625
60-64	22.385746007142014	28.221301468149157	27.168390682364695	22.22456184234413
65-69	22.427172529284913	28.22041271010405	27.17392248177234	22.178492278838704
70-74	22.402260086983652	28.216214266715646	27.16622210560277	22.21530354069793
75-79	22.357835633110316	28.221318229283305	27.161960859623736	22.25888527798265
80-84	22.393722295828873	28.26072668269106	27.143596928810272	22.201954092669798
85-89	22.371561563536392	28.262675828399946	27.139006350270378	22.22675625779328
90-94	22.356640386999636	28.23846643637013	27.124966968617496	22.27992620801274
95-99	22.37790459870652	28.24487180018004	27.15028162698887	22.22694197412457
100-104	22.377337709469213	28.280765161034378	27.124124140697038	22.21777298879937
105-109	22.334691943869462	28.262571189098523	27.14927594615024	22.253460920881775
110-114	22.34779858815482	28.322514240509456	27.129492310187764	22.20019486114796
115-119	22.388337197913092	28.296855237673963	27.10218827762379	22.21261928678916
120-124	22.373991878136824	28.261811520209328	27.098871020918274	22.26532558073557
125-129	22.354207322892467	28.325036815250755	27.125200485290758	22.195555376566023
130-134	22.387371001572593	28.344394449323048	27.0743407334827	22.193893815621664
135-139	22.338808885510026	28.334056720156408	27.052479909947913	22.27465448438565
140-144	22.334297152445597	28.390151143307513	27.08174177499939	22.1938099292475
145-149	22.366770160657495	28.429266623104432	27.029125798180196	22.174837418057876
150	22.24163683835453	28.12461520269346	26.933540957198854	22.700207001753153
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	44.0276151112867
2	42.19109302230669
3	43.36206878973871
4	44.20442253929552
5	44.01899418445569
6	44.437044876214536
7	44.16402465182953
8	42.9797504626197
9	43.1221627318083
10-14	43.881493392991835
15-19	44.02274409126032
20-24	44.176968548976866
25-29	44.313184658895146
30-34	44.414812818993916
35-39	44.42650495946287
40-44	44.5073851097321
45-49	44.56010909183009
50-54	44.53151309640067
55-59	44.564485027566555
60-64	44.61030784948614
65-69	44.60566480812361
70-74	44.617563627681584
75-79	44.61672091109296
80-84	44.595676388498674
85-89	44.59831782132967
90-94	44.636566595012376
95-99	44.60484657283109
100-104	44.595110698268584
105-109	44.58815286475124
110-114	44.54799344930278
115-119	44.600956484702245
120-124	44.6393174588724
125-129	44.54976269945849
130-134	44.58126481719425
135-139	44.613463369895676
140-144	44.52810708169309
145-149	44.54160757871537
150	44.94184384010769
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8.0
1	15.5
2	45.0
3	101.5
4	173.0
5	271.0
6	407.0
7	561.0
8	808.0
9	1121.5
10	1428.0
11	2044.5
12	2817.0
13	3660.0
14	7803.5
15	12668.0
16	23586.0
17	47435.5
18	58216.0
19	91569.0
20	136310.0
21	156158.0
22	168392.5
23	179119.5
24	246041.0
25	348652.5
26	427254.0
27	510557.5
28	624196.5
29	720262.0
30	816143.0
31	932204.0
32	1070751.0
33	1292046.5
34	1580257.5
35	1930868.0
36	2305561.5
37	2738087.0
38	3143460.5
39	3532460.0
40	4027936.5
41	4612702.0
42	5185942.0
43	5705789.0
44	6153774.5
45	6437488.0
46	6664822.5
47	6727854.5
48	6513972.0
49	6225148.5
50	5847629.0
51	5132615.0
52	4264094.0
53	3624078.0
54	3045651.5
55	2413069.0
56	1849082.5
57	1300204.5
58	848385.0
59	565276.5
60	409672.5
61	284190.5
62	162500.0
63	80328.0
64	31323.0
65	13721.0
66	6160.0
67	3909.0
68	2939.5
69	2491.0
70	2070.5
71	1759.0
72	1661.5
73	1476.0
74	1340.5
75	1298.0
76	1268.0
77	1272.0
78	1260.0
79	1157.5
80	1093.0
81	1106.5
82	1131.0
83	1110.5
84	1078.5
85	1092.5
86	1123.0
87	1127.0
88	1172.0
89	1206.0
90	1300.5
91	1476.0
92	1630.0
93	1953.0
94	2609.0
95	6532.0
96	10666.0
97	8730.0
98	4592.0
99	3218.5
100	9008.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03722255771782215
2	0.024076467154272235
3	0.012301837975605879
4	0.0029503933525491213
5	6.206154662439703E-4
6	0.011917433605776039
7	7.822808554247442E-4
8	1.5448026077273937E-4
9	2.442943658731692E-4
10-14	4.8445728291171867E-4
15-19	1.5088769656872216E-4
20-24	4.589500770631966E-4
25-29	0.003971400099330807
30-34	2.5668871237702855E-4
35-39	8.711968194741697E-5
40-44	2.8920141842338416E-5
45-49	2.3351667326111766E-5
50-54	5.029589885624072E-6
55-59	0.0
60-64	1.1118986211433216E-4
65-69	3.305159067695819E-5
70-74	2.3531295536312625E-5
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	1.5268397867073075E-5
100-104	5.227180916845018E-5
105-109	1.1406391347754593E-4
110-114	2.3890551956714344E-4
115-119	1.1783610589176397E-4
120-124	2.9459026472940993E-5
125-129	0.0
130-134	0.0
135-139	9.699923350846425E-6
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	1.11341086E8
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	87.3364137837614
#Duplication Level	Relative count
1	100.0
2	18.790670034170258
3	10.91368295944139
4	7.758134006834052
5	6.40320903283316
6	5.29490417471401
7	4.540187193581934
8	4.2163125835685635
9	3.6487891843708216
10++	432.69945030456097
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE