##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	N0067.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	99068890	
Filtered Sequences	0	
Sequence length	150	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.95463514328262	32.0	32.0	32.0	27.0	32.0
2	30.737318425592534	32.0	32.0	32.0	27.0	32.0
3	30.767178202965635	32.0	32.0	32.0	27.0	32.0
4	30.731855418991774	32.0	32.0	32.0	27.0	32.0
5	30.66763795375117	32.0	32.0	32.0	27.0	32.0
6	35.020183863975866	37.0	37.0	37.0	32.0	37.0
7	34.96998101018392	37.0	37.0	37.0	32.0	37.0
8	34.87563824526549	37.0	37.0	37.0	32.0	37.0
9	34.861965466656585	37.0	37.0	37.0	32.0	37.0
10-14	34.86115960519998	37.0	37.0	37.0	32.0	37.0
15-19	34.80785177869662	37.0	37.0	37.0	28.0	37.0
20-24	34.761826605708414	37.0	37.0	37.0	27.0	37.0
25-29	34.78952707555318	37.0	37.0	37.0	30.0	37.0
30-34	34.77710109399631	37.0	37.0	37.0	28.0	37.0
35-39	34.723831321820605	37.0	37.0	37.0	27.0	37.0
40-44	34.67455630521347	37.0	37.0	37.0	27.0	37.0
45-49	34.613746638324095	37.0	37.0	37.0	27.0	37.0
50-54	34.55257987648797	37.0	37.0	37.0	27.0	37.0
55-59	34.505521836370626	37.0	37.0	37.0	27.0	37.0
60-64	34.44922618190231	37.0	37.0	37.0	27.0	37.0
65-69	34.378374393818284	37.0	37.0	37.0	27.0	37.0
70-74	34.317948144972654	37.0	37.0	37.0	27.0	37.0
75-79	34.25288847588784	37.0	37.0	37.0	27.0	37.0
80-84	34.18823513415766	37.0	37.0	37.0	27.0	37.0
85-89	34.12738965178675	37.0	37.0	37.0	27.0	37.0
90-94	34.03925864920864	37.0	37.0	37.0	27.0	37.0
95-99	33.924523989316924	37.0	37.0	37.0	27.0	37.0
100-104	33.79384037915435	37.0	37.0	37.0	26.0	37.0
105-109	33.65094813720028	37.0	33.0	37.0	22.0	37.0
110-114	33.5144090824072	37.0	32.0	37.0	22.0	37.0
115-119	33.35146677226322	37.0	32.0	37.0	22.0	37.0
120-124	33.22728254651889	37.0	32.0	37.0	22.0	37.0
125-129	33.095769321731574	37.0	32.0	37.0	22.0	37.0
130-134	32.96149934050942	37.0	32.0	37.0	22.0	37.0
135-139	32.86678977830478	37.0	32.0	37.0	22.0	37.0
140-144	32.77487807726522	37.0	32.0	37.0	22.0	37.0
145-149	32.65454518567837	37.0	32.0	37.0	22.0	37.0
150	31.226659418511705	37.0	27.0	37.0	13.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	199.0
11	2823.0
12	10788.0
13	17743.0
14	21005.0
15	25083.0
16	29227.0
17	34838.0
18	48613.0
19	82828.0
20	149288.0
21	252381.0
22	389085.0
23	562588.0
24	766821.0
25	1005507.0
26	1263797.0
27	1569364.0
28	1929039.0
29	2389579.0
30	3009205.0
31	3927769.0
32	5456695.0
33	8481754.0
34	1.6015149E7
35	3.4769592E7
36	1.6858123E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.713081322738553	32.55542242605254	22.212029494647513	27.519466756561396
2	23.44473801257763	31.4059114166799	25.62409294004499	19.52525763069748
3	21.64155317378317	29.870603848237113	26.429114076603778	22.05872890137594
4	21.68261583573355	28.075342436438522	27.388208439999623	22.85383328782831
5	23.247752703335806	28.384423582889333	27.32452700714379	21.043296706631068
6	22.807196193060516	28.460766545522194	26.6167896977594	22.115247563657885
7	23.80538899431966	28.15166595034173	27.05225025406496	20.99069480127366
8	21.80422875242612	28.358569311051944	28.054614721781174	21.78258721474076
9	21.70480807889858	27.391236459024025	28.784412764752137	22.119542697325258
10-14	22.54357303510969	28.182507476149304	27.21382120107344	22.060098287667568
15-19	22.426670807198974	28.131195962800113	27.153011759805946	22.289121470194964
20-24	22.56116616418332	27.997362049491446	27.10961460917231	22.33185717715292
25-29	22.58008108685136	27.989329851625243	27.03253685538021	22.39805220614318
30-34	22.644494964980083	27.88215037808388	26.996933489285833	22.4764211676502
35-39	22.69479245198793	27.895711737193828	26.931402062358327	22.47809374845991
40-44	22.77028980323032	27.842912763793837	26.934085318828714	22.452712114147126
45-49	22.733165717264768	27.796376344805733	26.944475783475987	22.525982154453512
50-54	22.785168999754088	27.850568757493154	26.906542576792415	22.457719665960344
55-59	22.71608978358393	27.907576031183957	26.881652555105845	22.494681630126266
60-64	22.77479806459072	27.84419218527223	26.825455322193132	22.555554427943918
65-69	22.727015514062447	27.833926672179523	26.88733593799451	22.551721875763523
70-74	22.703994925291685	27.90539268752549	26.798140692159972	22.592471695022848
75-79	22.722503906120277	27.888655661731953	26.85562581754979	22.53321461459798
80-84	22.727444306683964	27.916889752171443	26.805486969723795	22.550178971420795
85-89	22.716018721921685	27.911094592863613	26.788291864378415	22.58459482083629
90-94	22.65784122543414	27.894199884544985	26.817964145959444	22.62999474406143
95-99	22.76243252749597	27.865673127565778	26.862555639826898	22.509338705111357
100-104	22.720155399013453	27.876806067376986	26.773825150269246	22.62921338334031
105-109	22.698522600572872	27.873801794076385	26.785679187443883	22.641996417906864
110-114	22.690809401562287	27.91298100545298	26.8317152486871	22.564494344297632
115-119	22.70278605403226	27.93146358281444	26.757869294908097	22.607881068245202
120-124	22.67517918087733	27.955422108799787	26.75594427409705	22.613454436225837
125-129	22.70733123183272	27.95682681011163	26.777059882269803	22.55878207578585
130-134	22.74245628471259	27.95531190467562	26.715455073737072	22.586776736874715
135-139	22.663257706462474	27.99777712636119	26.74384671952562	22.595118447650712
140-144	22.72706618596413	27.94358269281103	26.775230044467037	22.5541210767578
145-149	22.764850802305343	27.97085300945635	26.730450093869024	22.53384609436928
150	22.69290591627705	27.605161418483643	26.67970237680063	23.02223028843868
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	45.232548079299946
2	42.96999564327511
3	43.70028207515911
4	44.53644912356186
5	44.291049409966874
6	44.92244375671841
7	44.79608379559332
8	43.58681596716688
9	43.82435077622384
10-14	44.60367132277725
15-19	44.71579227739394
20-24	44.89302334133624
25-29	44.97813329299454
30-34	45.120916132630285
35-39	45.172886200447834
40-44	45.223001917377445
45-49	45.259147871718284
50-54	45.24288866571443
55-59	45.2107714137102
60-64	45.33035249253464
65-69	45.27873738982597
70-74	45.29646662031453
75-79	45.25571852071826
80-84	45.27762327810476
85-89	45.30061354275797
90-94	45.287835969495575
95-99	45.27177123260733
100-104	45.349368782353764
105-109	45.34051901847974
110-114	45.25530374585992
115-119	45.31066712227746
120-124	45.288633617103166
125-129	45.26611330761857
130-134	45.3292330215873
135-139	45.258376154113186
140-144	45.28118726272193
145-149	45.29869689667463
150	45.715136204715726
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19.0
1	19.5
2	34.0
3	78.0
4	138.0
5	210.5
6	346.0
7	500.0
8	656.5
9	857.5
10	1092.0
11	1820.5
12	3004.0
13	4628.5
14	8026.5
15	12798.5
16	14889.5
17	17721.0
18	23550.5
19	33043.0
20	52765.5
21	77630.5
22	111229.0
23	165443.5
24	202197.0
25	226923.5
26	263989.5
27	323965.5
28	422347.0
29	557131.0
30	692040.0
31	784341.0
32	884553.5
33	1117343.5
34	1415691.0
35	1573976.0
36	1757964.5
37	2006830.5
38	2287538.0
39	2665760.0
40	3040893.5
41	3537194.0
42	4161293.0
43	4723240.5
44	5291350.5
45	5970950.5
46	6629364.0
47	6856078.0
48	6574022.5
49	6060189.0
50	5510632.5
51	4797389.0
52	3961324.5
53	3243415.5
54	2671487.0
55	2190138.5
56	1721044.5
57	1344452.5
58	1027149.0
59	713646.5
60	454343.5
61	284461.5
62	183664.0
63	114703.0
64	74954.5
65	65498.0
66	42419.5
67	22679.0
68	12981.0
69	5668.5
70	2934.5
71	1884.5
72	1320.5
73	1186.0
74	1080.0
75	1047.0
76	1036.5
77	998.0
78	940.5
79	889.0
80	861.5
81	820.5
82	809.5
83	868.0
84	842.0
85	801.0
86	790.0
87	776.0
88	833.5
89	873.0
90	904.5
91	1007.0
92	1130.0
93	1378.5
94	1820.5
95	5033.5
96	8519.0
97	6947.5
98	3489.0
99	2207.5
100	8148.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03785042913067866
2	0.02453545204756004
3	0.012427715703688615
4	0.002941387553650798
5	6.450057126914413E-4
6	0.01179078517988846
7	7.277763988271192E-4
8	1.453534000431417E-4
9	2.3922747090433737E-4
10-14	5.147932918194602E-4
15-19	1.477759567105274E-4
20-24	4.7017787319510694E-4
25-29	0.003987932033961418
30-34	2.674906320238372E-4
35-39	9.326843169434926E-5
40-44	2.907068000862834E-5
45-49	2.7253762508089067E-5
50-54	6.258271390746379E-6
55-59	0.0
60-64	1.0921692975463841E-4
65-69	3.5530831121656856E-5
70-74	2.3821807229292668E-5
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	1.6150377782571298E-5
100-104	5.046993057053531E-5
105-109	1.1022632836604913E-4
110-114	2.598192025771158E-4
115-119	1.233485103143883E-4
120-124	3.1695116398296174E-5
125-129	0.0
130-134	0.0
135-139	1.0901505003235628E-5
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	9.906889E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	88.45664395367193
#Duplication Level	Relative count
1	100.0
2	18.180295841473626
3	11.627128104939995
4	9.00362824448786
5	7.828635221881105
6	7.033212391850404
7	6.382919341334078
8	5.584705554005024
9	5.30560982416969
10++	387.24532514652526
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE