##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	N0069.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	106850812	
Filtered Sequences	0	
Sequence length	150	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.44294942746902	32.0	27.0	32.0	27.0	32.0
2	30.622559985786538	32.0	32.0	32.0	27.0	32.0
3	30.577202866740965	32.0	32.0	32.0	27.0	32.0
4	30.639251707324416	32.0	32.0	32.0	27.0	32.0
5	30.536708190855865	32.0	32.0	32.0	27.0	32.0
6	34.78183564014469	37.0	37.0	37.0	32.0	37.0
7	34.75317340592601	37.0	37.0	37.0	27.0	37.0
8	34.68592821737283	37.0	37.0	37.0	27.0	37.0
9	34.69263705735807	37.0	37.0	37.0	27.0	37.0
10-14	34.66241602169574	37.0	37.0	37.0	27.0	37.0
15-19	34.6068231021024	37.0	37.0	37.0	27.0	37.0
20-24	34.55066513673289	37.0	37.0	37.0	27.0	37.0
25-29	34.590310501337136	37.0	37.0	37.0	27.0	37.0
30-34	34.5721108885911	37.0	37.0	37.0	27.0	37.0
35-39	34.51131700899007	37.0	37.0	37.0	27.0	37.0
40-44	34.46118284810039	37.0	37.0	37.0	27.0	37.0
45-49	34.39723865271141	37.0	37.0	37.0	27.0	37.0
50-54	34.32934810453289	37.0	37.0	37.0	27.0	37.0
55-59	34.28461797744691	37.0	37.0	37.0	27.0	37.0
60-64	34.225952686255674	37.0	37.0	37.0	27.0	37.0
65-69	34.148235317107364	37.0	37.0	37.0	27.0	37.0
70-74	34.07976234752432	37.0	37.0	37.0	27.0	37.0
75-79	34.00589234268055	37.0	37.0	37.0	27.0	37.0
80-84	33.9366501211053	37.0	37.0	37.0	27.0	37.0
85-89	33.871590742801274	37.0	37.0	37.0	23.0	37.0
90-94	33.779216717604356	37.0	37.0	37.0	22.0	37.0
95-99	33.661703787520125	37.0	37.0	37.0	22.0	37.0
100-104	33.52110691119502	37.0	32.0	37.0	22.0	37.0
105-109	33.37278235003025	37.0	32.0	37.0	22.0	37.0
110-114	33.22523816103522	37.0	32.0	37.0	22.0	37.0
115-119	33.060347050989186	37.0	32.0	37.0	22.0	37.0
120-124	32.92900466867767	37.0	32.0	37.0	22.0	37.0
125-129	32.78540903367211	37.0	32.0	37.0	22.0	37.0
130-134	32.64831202593014	37.0	32.0	37.0	22.0	37.0
135-139	32.55461616520051	37.0	32.0	37.0	22.0	37.0
140-144	32.45707417553364	37.0	32.0	37.0	18.4	37.0
145-149	32.33272463666443	37.0	32.0	37.0	13.0	37.0
150	30.942900396489264	37.0	27.0	37.0	13.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	206.0
11	3236.0
12	11339.0
13	18749.0
14	22885.0
15	26004.0
16	29912.0
17	36889.0
18	54245.0
19	98188.0
20	183615.0
21	310180.0
22	476631.0
23	687706.0
24	942142.0
25	1237153.0
26	1566058.0
27	1938890.0
28	2375761.0
29	2933598.0
30	3674933.0
31	4773779.0
32	6579689.0
33	1.009778E7
34	1.8535095E7
35	3.611704E7
36	1.4119107E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.791173242337752	31.70752197000864	22.014569330664575	27.486735456989038
2	24.404035899768864	31.33539019272582	25.65537399137282	18.6051999161325
3	20.45721072049674	29.69101291943077	26.968258859345852	22.883517500726636
4	21.79115733980206	27.40774918170165	27.904522891534445	22.896570586961847
5	23.677149579348228	28.14172874535735	27.448755377367878	20.73236629792654
6	22.464573824617332	28.26833329210275	26.623688765595865	22.643404117684053
7	24.249605790846708	27.936957045533127	27.007081562607322	20.80635560101284
8	22.230210630142942	27.76064249148632	27.978480170562538	22.0306667078082
9	22.097121307896455	26.93987464995263	28.640620415032448	22.32238362711847
10-14	22.747340171841255	27.9931162811484	26.900836188267697	22.358707358742652
15-19	22.6739965342988	27.84353193960108	26.952098274044445	22.530373252055668
20-24	22.821105499636776	27.749382966232076	26.855952529702982	22.57355900442817
25-29	22.83446869015318	27.7603016001441	26.78699378448306	22.618235925219665
30-34	22.863735570710983	27.63176020403293	26.723973492478432	22.780530732777656
35-39	22.925775991239668	27.647109884319644	26.686514605989142	22.74059951845155
40-44	22.976948861133923	27.653730756900202	26.64016250235864	22.729157879607236
45-49	22.977497054480924	27.59439415734559	26.62401225191285	22.804096536260637
50-54	22.9463067333205	27.635217567325483	26.63943583781459	22.779039861539424
55-59	22.992008708366203	27.645383546547126	26.55730852096847	22.8052992241182
60-64	22.944155041627997	27.631053255587958	26.56665688728768	22.85813481549636
65-69	22.96101952293366	27.673020330744368	26.552742826422204	22.813217319899767
70-74	22.953502923485964	27.695015600595173	26.56580940447694	22.78567207144192
75-79	22.92820105101307	27.66210049952639	26.543735764965454	22.865962684495088
80-84	22.914992166835383	27.682584948441946	26.589417401900512	22.81300548282216
85-89	22.948807352067664	27.743288464667913	26.510261803157846	22.797642380106574
90-94	22.861338292871373	27.74617136274079	26.559634006337735	22.832856338050103
95-99	22.88334184567024	27.799418140055277	26.52855258480566	22.788687429468823
100-104	22.877802033312726	27.827029766893084	26.503498828810336	22.791669370983854
105-109	22.838048700136802	27.811057308789984	26.512500136428685	22.838393854644533
110-114	22.866907699633064	27.82095918848782	26.50032573344531	22.8118073784338
115-119	22.850514451694785	27.87429301534674	26.472071877853093	22.80312065510538
120-124	22.864619270131758	27.808350631662577	26.466831572526374	22.860198525679294
125-129	22.86709005075226	27.874733043675885	26.470306655226917	22.787870250344938
130-134	22.85390703975915	27.88441051413706	26.46250554738415	22.799176898719644
135-139	22.83946004875905	27.845730492943154	26.486837952054486	22.82797150624331
140-144	22.86853898686329	27.927833810004177	26.41774533262321	22.78588187050932
145-149	22.871242943853343	27.94274450623735	26.431139147543398	22.75487340236591
150	22.878079766019933	27.503594450924716	26.302006015639822	23.316319767415525
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	46.27790869932679
2	43.009235815901356
3	43.34072822122338
4	44.687727926763905
5	44.40951587727477
6	45.10797794230139
7	45.05596139185955
8	44.26087733795114
9	44.41950493501493
10-14	45.1060475305839
15-19	45.20436978635447
20-24	45.39466450406494
25-29	45.45270461537284
30-34	45.64426630348864
35-39	45.666375509691214
40-44	45.70610674074116
45-49	45.781593590741565
50-54	45.72534659485993
55-59	45.79730793248441
60-64	45.80228985712436
65-69	45.77423684283343
70-74	45.73917499492788
75-79	45.79416373550816
80-84	45.727997649657546
85-89	45.74644973217424
90-94	45.69419463092147
95-99	45.67202927513907
100-104	45.66947140429658
105-109	45.67644255478133
110-114	45.678715078066865
115-119	45.65363510680017
120-124	45.72481779581105
125-129	45.6549603010972
130-134	45.65308393847879
135-139	45.667431555002366
140-144	45.654420857372614
145-149	45.62611634621925
150	46.19439953343546
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	14.0
2	39.5
3	91.0
4	178.0
5	283.5
6	399.5
7	515.0
8	652.0
9	851.0
10	1075.5
11	1323.5
12	1649.5
13	2184.5
14	2996.5
15	6457.0
16	11168.5
17	14877.0
18	19322.0
19	21860.0
20	24917.5
21	34907.0
22	61119.0
23	121077.0
24	173170.0
25	213068.0
26	276558.0
27	337159.5
28	391478.0
29	424867.0
30	504750.0
31	657304.0
32	818215.0
33	1044785.0
34	1300173.5
35	1524754.0
36	1814616.5
37	2221711.0
38	2606441.0
39	2951631.0
40	3466542.0
41	4031352.5
42	4565163.0
43	5276135.5
44	5911199.5
45	6217863.5
46	6352220.0
47	6295183.5
48	6169080.0
49	6141247.5
50	5989191.5
51	5589232.5
52	4978767.0
53	4288177.0
54	3671078.0
55	2979168.5
56	2263732.0
57	1679051.0
58	1193311.0
59	817377.0
60	531499.5
61	335118.5
62	210225.0
63	121830.5
64	58046.0
65	27619.5
66	14694.5
67	7230.0
68	4503.5
69	3272.0
70	2339.5
71	1889.0
72	1717.5
73	1557.0
74	1436.5
75	1187.5
76	1041.5
77	957.0
78	887.5
79	870.0
80	855.0
81	834.0
82	812.0
83	791.5
84	784.5
85	801.0
86	787.5
87	754.5
88	820.5
89	899.5
90	951.0
91	1044.5
92	1211.0
93	1483.0
94	1982.0
95	5139.0
96	8611.5
97	6939.5
98	3311.5
99	2301.0
100	9679.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.036667011945590085
2	0.023552464907800608
3	0.011783719528495488
4	0.00296581742401733
5	6.307860346442664E-4
6	0.011838000819310572
7	7.552586497892033E-4
8	1.4131853298410125E-4
9	2.5643230488505786E-4
10-14	5.096826030671625E-4
15-19	1.5853880455302485E-4
20-24	4.6887804652340876E-4
25-29	0.004010077153180642
30-34	2.755243451027775E-4
35-39	8.32937048714239E-5
40-44	2.8638060326579455E-5
45-49	3.331748194856957E-5
50-54	5.9896596761473366E-6
55-59	0.0
60-64	1.1979319352294676E-4
65-69	3.425336627296758E-5
70-74	1.572285664988676E-5
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	1.2166496217174277E-5
100-104	5.334540649068722E-5
105-109	1.0687798984625405E-4
110-114	2.3883767958637504E-4
115-119	1.1155741146824416E-4
120-124	2.845088346169985E-5
125-129	0.0
130-134	1.8717686487960427E-7
135-139	7.86142832494338E-6
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	1.06850812E8
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	87.24993543516699
#Duplication Level	Relative count
1	100.0
2	17.85914615144905
3	10.07790588968526
4	7.270177625428483
5	5.462760984730446
6	4.8800249298847
7	4.306637581801184
8	3.910875662200062
9	3.711436584605796
10++	465.7681520722967
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE