##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	N0071.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	146631640	
Filtered Sequences	0	
Sequence length	150	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.195085658184006	32.0	32.0	32.0	27.0	32.0
2	30.797251063958637	32.0	32.0	32.0	27.0	32.0
3	30.732438674217924	32.0	32.0	32.0	27.0	32.0
4	30.740466129956673	32.0	32.0	32.0	27.0	32.0
5	30.702588043071742	32.0	32.0	32.0	27.0	32.0
6	35.050186058070416	37.0	37.0	37.0	32.0	37.0
7	34.943922825933065	37.0	37.0	37.0	32.0	37.0
8	34.92821789349147	37.0	37.0	37.0	32.0	37.0
9	34.9197790736024	37.0	37.0	37.0	32.0	37.0
10-14	34.87710519366762	37.0	37.0	37.0	32.0	37.0
15-19	34.817978253533816	37.0	37.0	37.0	29.0	37.0
20-24	34.78020848979115	37.0	37.0	37.0	27.0	37.0
25-29	34.81266845272957	37.0	37.0	37.0	31.0	37.0
30-34	34.782859725227105	37.0	37.0	37.0	30.0	37.0
35-39	34.725663380700105	37.0	37.0	37.0	27.0	37.0
40-44	34.682389107835114	37.0	37.0	37.0	27.0	37.0
45-49	34.627322805637306	37.0	37.0	37.0	27.0	37.0
50-54	34.56580651079126	37.0	37.0	37.0	27.0	37.0
55-59	34.527690602110155	37.0	37.0	37.0	27.0	37.0
60-64	34.471279280515446	37.0	37.0	37.0	27.0	37.0
65-69	34.40224485111126	37.0	37.0	37.0	27.0	37.0
70-74	34.34219118738629	37.0	37.0	37.0	27.0	37.0
75-79	34.27565722104725	37.0	37.0	37.0	27.0	37.0
80-84	34.21584163417936	37.0	37.0	37.0	27.0	37.0
85-89	34.15638940135976	37.0	37.0	37.0	27.0	37.0
90-94	34.07116211617084	37.0	37.0	37.0	27.0	37.0
95-99	33.96206771744488	37.0	37.0	37.0	27.0	37.0
100-104	33.83070297242805	37.0	37.0	37.0	27.0	37.0
105-109	33.69492577318237	37.0	35.0	37.0	22.0	37.0
110-114	33.556539645877244	37.0	32.0	37.0	22.0	37.0
115-119	33.40150286254727	37.0	32.0	37.0	22.0	37.0
120-124	33.28301672544889	37.0	32.0	37.0	22.0	37.0
125-129	33.1478706628392	37.0	32.0	37.0	22.0	37.0
130-134	33.0220395107086	37.0	32.0	37.0	22.0	37.0
135-139	32.9347622764091	37.0	32.0	37.0	22.0	37.0
140-144	32.845268703262136	37.0	32.0	37.0	22.0	37.0
145-149	32.727027595135674	37.0	32.0	37.0	22.0	37.0
150	31.341678221698945	37.0	27.0	37.0	13.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	416.0
11	5955.0
12	19389.0
13	28907.0
14	32999.0
15	37823.0
16	44747.0
17	53437.0
18	74921.0
19	127802.0
20	224813.0
21	357690.0
22	521792.0
23	721899.0
24	968617.0
25	1258962.0
26	1603344.0
27	2023510.0
28	2552881.0
29	3258941.0
30	4258471.0
31	5807134.0
32	8500647.0
33	1.392142E7
34	2.6696752E7
35	5.2500247E7
36	2.1028123E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.106560268843218	32.52100105697757	21.801408018991758	27.571030655187457
2	23.919133279694353	32.08354747325623	25.476078232271444	18.52124101477797
3	20.1260126306055	29.988874084816047	26.778328555250862	23.10678472932759
4	21.5510036971723	27.436595424447663	28.217181186731295	22.79521969164874
5	23.463930916876926	28.25460222129122	27.814850401581424	20.466616460250425
6	22.24341012410092	28.38501525494832	26.85525957652658	22.516315044424182
7	24.007616830969074	28.16794741968308	27.216278655738385	20.608157093609456
8	21.936159301632877	27.930659825313285	28.243027401440358	21.890153471613477
9	21.889911703208888	26.971239414339625	28.995144098295057	22.14370478415643
10-14	22.564629510834237	28.08780308339811	27.218083049582866	22.129484356184793
15-19	22.415656979108505	27.999690936662525	27.24126978591455	22.343382298314417
20-24	22.59561278079176	27.87063151681013	27.147293049353472	22.386462653044642
25-29	22.632496604460066	27.86042094631452	27.125258482530334	22.381823966695077
30-34	22.698596883162935	27.69925312804491	27.065344414180863	22.536805574611297
35-39	22.72002976647822	27.754473458653006	27.007298099254946	22.518198675613828
40-44	22.747893800173895	27.736504720314727	27.00321298683669	22.512388492674688
45-49	22.756580368321174	27.68724257996275	26.99033141983904	22.56584563187703
50-54	22.733207177925173	27.755466099580655	26.97435373828303	22.536972984211136
55-59	22.76012134898034	27.71973484031141	26.972785000563317	22.54735881014493
60-64	22.73970980557341	27.699115727436602	26.95941034110505	22.60176412588494
65-69	22.76484814540534	27.73967871378076	26.929220115684917	22.566253025128987
70-74	22.75841666252204	27.744126810831887	26.975162480106064	22.52229404654001
75-79	22.717782328561558	27.740836698000514	26.947654953596643	22.593726019841284
80-84	22.72431216073148	27.76495986814306	26.93648123965605	22.574246731469415
85-89	22.725145132387524	27.794184802134108	26.926131631617846	22.55453843386052
90-94	22.706065348515505	27.793450308541868	26.926090167169924	22.574394175772706
95-99	22.685288425130505	27.82056855498353	26.95554859949958	22.538594420386385
100-104	22.710431071842173	27.830560109494133	26.912563591767707	22.546445226895987
105-109	22.717636193504706	27.76276348506961	26.92133563979778	22.598264681627906
110-114	22.73332498340903	27.793717783909464	26.90338927121093	22.56956796147057
115-119	22.71396367134152	27.848936046158812	26.892913405435632	22.54418687706404
120-124	22.71506094844295	27.803014947293274	26.912953352604923	22.568970751658853
125-129	22.704119141958884	27.809280217842865	26.91118673556565	22.575413904632608
130-134	22.71739491988521	27.835683599401946	26.88879641619829	22.558125064514556
135-139	22.675856634751998	27.837856948054114	26.892072603980466	22.594213813213422
140-144	22.725307307481522	27.85694424477555	26.87846606639604	22.53928238134689
145-149	22.753380921061783	27.877872606485205	26.855034834228135	22.513711638224873
150	22.784682760146445	27.363899769517687	26.817055309481635	23.034362160854233
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	45.67759092403068
2	42.44037429447232
3	43.232797359933095
4	44.34622338882104
5	43.930547377127354
6	44.7597251685251
7	44.615773924578534
8	43.82631277324636
9	44.033616487365315
10-14	44.69411386701903
15-19	44.75903927742292
20-24	44.98207543383641
25-29	45.01432057115514
30-34	45.235402457774235
35-39	45.23822844209205
40-44	45.260282292848586
45-49	45.3224260001982
50-54	45.27018016213631
55-59	45.30748015912528
60-64	45.341473931458346
65-69	45.331101170534325
70-74	45.28071070906205
75-79	45.31150834840284
80-84	45.29855889220089
85-89	45.279683566248046
90-94	45.280459524288204
95-99	45.22388284551689
100-104	45.256876298738156
105-109	45.31590087513261
110-114	45.3028929448796
115-119	45.25815054840556
120-124	45.28403170010181
125-129	45.27953304659149
130-134	45.275519984399764
135-139	45.27007044796542
140-144	45.264589688828416
145-149	45.26709255928666
150	45.81904492100068
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	22.0
2	44.0
3	73.5
4	124.0
5	175.0
6	256.5
7	395.0
8	552.0
9	719.5
10	908.5
11	1187.0
12	1580.0
13	2039.5
14	2830.0
15	7092.5
16	14617.5
17	22652.5
18	32457.5
19	34848.0
20	35709.5
21	48243.5
22	73049.0
23	101909.0
24	133752.0
25	168997.0
26	222039.5
27	301869.0
28	383879.5
29	451415.5
30	533546.5
31	687525.0
32	864481.5
33	1104932.5
34	1480311.5
35	1913378.5
36	2427612.5
37	3054434.5
38	3727858.5
39	4371912.0
40	5235834.5
41	6298340.0
42	7263056.5
43	8274051.0
44	9215561.0
45	9965864.5
46	1.04048835E7
47	1.0366127E7
48	9924579.0
49	9137334.5
50	8159022.5
51	7067916.0
52	5918933.5
53	4819147.5
54	3718907.0
55	2695565.5
56	1927469.0
57	1374808.0
58	959341.0
59	673913.5
60	417852.5
61	211449.5
62	125798.0
63	74892.0
64	37233.0
65	15588.5
66	7080.0
67	4107.5
68	3248.0
69	2788.0
70	2481.5
71	2304.5
72	2168.0
73	2084.0
74	1999.0
75	1897.5
76	1859.5
77	1805.5
78	1740.0
79	1705.5
80	1667.0
81	1618.5
82	1596.5
83	1573.0
84	1503.5
85	1491.5
86	1531.5
87	1566.5
88	1610.5
89	1660.5
90	1763.5
91	1857.5
92	2030.0
93	2432.0
94	3195.0
95	8886.0
96	15269.5
97	12726.5
98	6555.5
99	4257.5
100	14433.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03765080987977765
2	0.02421919307456426
3	0.012332263350529258
4	0.0030320877540481713
5	6.676594492157355E-4
6	0.01198513499542118
7	7.53589061678639E-4
8	1.527637554896065E-4
9	2.57106856337418E-4
10-14	5.135317316235431E-4
15-19	1.4130647382788598E-4
20-24	4.768411510639859E-4
25-29	0.004135260302619544
30-34	2.7361079777870587E-4
35-39	8.061016026281913E-5
40-44	2.741563826197402E-5
45-49	2.5778883738871094E-5
50-54	5.728640830860242E-6
55-59	0.0
60-64	1.0502508189910445E-4
65-69	2.8097619313266905E-5
70-74	1.85498845951665E-5
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	1.1593677871979063E-5
100-104	5.5649653785499495E-5
105-109	1.1743713703263498E-4
110-114	2.5274217760914357E-4
115-119	1.196194763967722E-4
120-124	2.400573300550959E-5
125-129	1.363962102585772E-7
130-134	4.091886307757316E-7
135-139	6.81981051292886E-6
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	1.4663164E8
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	89.35977185292936
#Duplication Level	Relative count
1	100.0
2	19.308533601957116
3	11.026766441214564
4	8.029932364368975
5	6.328248668873219
6	5.2273708447258596
7	4.662541372859404
8	4.086918981148367
9	3.784717225500072
10++	557.0729601381494
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE