##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	N0073.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	117997478	
Filtered Sequences	0	
Sequence length	150	
%GC	46	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.113874391450977	32.0	32.0	32.0	27.0	32.0
2	30.737659570995238	32.0	32.0	32.0	27.0	32.0
3	30.644095800081423	32.0	32.0	32.0	27.0	32.0
4	30.517826397950643	32.0	32.0	32.0	27.0	32.0
5	30.496225749841873	32.0	32.0	32.0	27.0	32.0
6	34.78688125012299	37.0	37.0	37.0	32.0	37.0
7	34.838019410889444	37.0	37.0	37.0	32.0	37.0
8	34.81533279041778	37.0	37.0	37.0	32.0	37.0
9	34.81274054009866	37.0	37.0	37.0	32.0	37.0
10-14	34.780276851340844	37.0	37.0	37.0	27.0	37.0
15-19	34.72578829523796	37.0	37.0	37.0	27.0	37.0
20-24	34.68537306704131	37.0	37.0	37.0	27.0	37.0
25-29	34.72166100702593	37.0	37.0	37.0	27.0	37.0
30-34	34.69304966501063	37.0	37.0	37.0	27.0	37.0
35-39	34.634499405148304	37.0	37.0	37.0	27.0	37.0
40-44	34.58512723466852	37.0	37.0	37.0	27.0	37.0
45-49	34.525266323064976	37.0	37.0	37.0	27.0	37.0
50-54	34.463717780476635	37.0	37.0	37.0	27.0	37.0
55-59	34.42045342528423	37.0	37.0	37.0	27.0	37.0
60-64	34.36492275877286	37.0	37.0	37.0	27.0	37.0
65-69	34.289710147872825	37.0	37.0	37.0	27.0	37.0
70-74	34.227498625012984	37.0	37.0	37.0	27.0	37.0
75-79	34.16087104166752	37.0	37.0	37.0	27.0	37.0
80-84	34.10002615310134	37.0	37.0	37.0	27.0	37.0
85-89	34.0390251222149	37.0	37.0	37.0	27.0	37.0
90-94	33.94642658888014	37.0	37.0	37.0	27.0	37.0
95-99	33.83505114066929	37.0	37.0	37.0	24.0	37.0
100-104	33.698407215110144	37.0	37.0	37.0	22.0	37.0
105-109	33.5575920393824	37.0	32.0	37.0	22.0	37.0
110-114	33.41284836104718	37.0	32.0	37.0	22.0	37.0
115-119	33.25488520695332	37.0	32.0	37.0	22.0	37.0
120-124	33.13015550722194	37.0	32.0	37.0	22.0	37.0
125-129	32.9913070192907	37.0	32.0	37.0	22.0	37.0
130-134	32.860487662287156	37.0	32.0	37.0	22.0	37.0
135-139	32.7693188510351	37.0	32.0	37.0	22.0	37.0
140-144	32.67937178284437	37.0	32.0	37.0	22.0	37.0
145-149	32.55916650015181	37.0	32.0	37.0	22.0	37.0
150	31.094308193603933	37.0	27.0	37.0	13.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	346.0
11	4794.0
12	16323.0
13	24925.0
14	27638.0
15	30139.0
16	34229.0
17	42342.0
18	60722.0
19	105057.0
20	185315.0
21	299616.0
22	449158.0
23	633766.0
24	852985.0
25	1113853.0
26	1419737.0
27	1792794.0
28	2252978.0
29	2859243.0
30	3707761.0
31	4998105.0
32	7193466.0
33	1.1580049E7
34	2.178757E7
35	4.1148171E7
36	1.5376395E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.08667940534503	31.190987299571297	21.473789943893383	28.248543351190285
2	25.690002722834173	30.501029715512274	25.47036703908063	18.33860052257292
3	21.300289186349776	28.990028257692817	26.243747483378826	23.465935072578578
4	22.16960754864715	27.1683832905581	27.38173328283016	23.280275877964584
5	24.196329985126365	27.704085219747864	27.098579241478372	21.001005553647403
6	23.025582063597046	27.433228500625574	26.287577713169917	23.25361172260746
7	24.705606333009918	27.379046212130497	26.581818115868668	21.33352933899092
8	22.385149870126686	27.23466684804935	27.645684734238575	22.734498547585385
9	22.619795942193587	26.24940642635171	28.24390371726938	22.88689391418532
10-14	23.310544368163253	27.329368078174067	26.42518187185367	22.934905681809013
15-19	23.251370682655175	27.219603745130495	26.401183650152422	23.127841922061908
20-24	23.374515029439173	27.09566738922743	26.312737529218204	23.217080052115197
25-29	23.414804149634207	27.07039190888032	26.26761427316424	23.247189668321226
30-34	23.520232569426366	26.96695956290928	26.169830266976057	23.342977600688293
35-39	23.550819141138486	26.98682979120636	26.13340093935992	23.32895012829523
40-44	23.568587470982802	26.982154829376242	26.119228291171737	23.330029408469223
45-49	23.584091235931588	26.912742253009075	26.109381437057	23.393785074002334
50-54	23.53591136547859	26.97509788040072	26.086664113120467	23.40232664100022
55-59	23.562574278070585	26.96285186705431	26.08155235317826	23.393021501696843
60-64	23.55869358746662	26.944707101461024	26.082169634795054	23.414429676277305
65-69	23.563650193458344	26.98735572774283	26.08033302524926	23.368661053549573
70-74	23.575199443417134	26.97024987788762	26.060455993677657	23.394094685017595
75-79	23.51861960981912	26.975688582089862	26.077607353608016	23.428084454483002
80-84	23.50354945721806	27.019367312240348	26.07974994177418	23.397333288767406
85-89	23.554498851238158	27.00442326402942	26.083807316627567	23.357270568104855
90-94	23.495150464148058	27.017458288388163	26.07508899469868	23.412302252765098
95-99	23.5298300189817	27.003980465423183	26.083230966156002	23.38295854943912
100-104	23.54115644857458	27.07213833539494	26.021452110370102	23.365253105660372
105-109	23.525996433774154	26.994441609079455	26.065920758278182	23.41364119886821
110-114	23.489952799927806	27.056235110895738	26.058596119614112	23.39521596956234
115-119	23.540902023723586	27.0580397829307	26.03923596921761	23.361822224128105
120-124	23.52916049305104	27.024872124555177	26.055268877488096	23.39069850490569
125-129	23.46519287471551	27.00326612065387	26.11902671343535	23.412514291195276
130-134	23.532958680013273	27.061328346668535	26.05190676728823	23.35380620602996
135-139	23.501742965380775	27.06300958138364	26.026295293271	23.408952159964585
140-144	23.52696114403394	27.034654757621173	26.040090619563923	23.398293478780964
145-149	23.551209119910173	27.037546514341603	26.04223761460393	23.36900675114429
150	23.595386504786145	26.36329990035889	26.030697876441057	24.010615718413913
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	47.33522275653532
2	44.0286032454071
3	44.766224258928354
4	45.449883426611734
5	45.197335538773764
6	46.2791937862045
7	46.03913567200084
8	45.11964841771207
9	45.506689856378905
10-14	46.24545004997227
15-19	46.37921260471708
20-24	46.59159508155437
25-29	46.661993817955434
30-34	46.86321017011466
35-39	46.879769269433716
40-44	46.89861687945202
45-49	46.977876309933926
50-54	46.93823800647881
55-59	46.95559577976743
60-64	46.97312326374393
65-69	46.93231124700792
70-74	46.96929412843473
75-79	46.94670406430212
80-84	46.90088274598547
85-89	46.91176941934302
90-94	46.90745271691315
95-99	46.912788568420815
100-104	46.906409554234955
105-109	46.93963763264236
110-114	46.88516876949015
115-119	46.902724247851694
120-124	46.91985899795672
125-129	46.877707165910785
130-134	46.88676488604323
135-139	46.910695125345356
140-144	46.925254622814904
145-149	46.92021587105447
150	47.606002223200065
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14.0
1	16.5
2	26.0
3	57.0
4	109.5
5	162.0
6	241.0
7	347.5
8	455.5
9	585.0
10	728.5
11	945.0
12	1221.5
13	1626.5
14	2247.5
15	3574.5
16	8919.5
17	13793.0
18	13235.0
19	17070.0
20	31966.0
21	100075.5
22	171285.5
23	216066.5
24	268858.5
25	330320.0
26	426804.0
27	516188.5
28	599943.0
29	767093.0
30	993424.0
31	1213278.0
32	1453593.5
33	1577774.0
34	1670277.5
35	1894162.0
36	2156722.5
37	2356388.0
38	2463107.0
39	2658639.5
40	2976744.0
41	3288868.5
42	3605031.5
43	3998453.5
44	4366695.0
45	4658535.5
46	4939846.0
47	5302760.0
48	5727686.0
49	5942006.5
50	5852211.5
51	5693643.5
52	5627920.5
53	5507860.0
54	5185591.5
55	4712917.5
56	4145706.5
57	3479963.0
58	2834868.5
59	2285512.5
60	1810341.0
61	1366647.0
62	962233.0
63	661087.0
64	432100.5
65	259028.5
66	151189.5
67	87595.0
68	50050.0
69	29652.5
70	16656.0
71	8041.5
72	4386.5
73	2927.0
74	2212.0
75	1940.0
76	2015.5
77	2082.0
78	1804.5
79	1502.5
80	1433.5
81	1403.0
82	1378.5
83	1372.0
84	1351.0
85	1333.5
86	1327.5
87	1326.5
88	1339.0
89	1417.0
90	1510.5
91	1613.0
92	1814.5
93	2186.0
94	2813.0
95	7532.0
96	12431.5
97	9965.5
98	5064.5
99	3317.0
100	9813.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03760504101621562
2	0.024142041408715533
3	0.012190938521584335
4	0.0030229459649976586
5	6.695058346925008E-4
6	0.012356196290907166
7	7.491685542635072E-4
8	1.5254563322107613E-4
9	2.7627709127817123E-4
10-14	5.361131531980708E-4
15-19	1.523761380730527E-4
20-24	4.859425893831392E-4
25-29	0.004176699437593064
30-34	2.8339588749515477E-4
35-39	9.152737993264568E-5
40-44	3.457701019677726E-5
45-49	3.1865087828402565E-5
50-54	3.7288932565151943E-6
55-59	0.0
60-64	1.0390052573835519E-4
65-69	2.9322660608051302E-5
70-74	2.220386439106775E-5
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	1.728850509838863E-5
100-104	5.220450559121272E-5
105-109	1.0830739958696405E-4
110-114	2.4203907137744081E-4
115-119	1.1695165213615836E-4
120-124	3.3051553864566494E-5
125-129	0.0
130-134	5.084854440702538E-7
135-139	9.322233141287985E-6
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	1.17997478E8
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	88.49269476354199
#Duplication Level	Relative count
1	100.0
2	18.471682487099446
3	11.17729502452698
4	8.711855768618207
5	7.444097598267184
6	6.555392750207046
7	6.351532139899343
8	5.472383257947379
9	4.83531885073581
10++	468.04484933426767
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE