##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane1_Undetermined_L001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4239454	
Filtered Sequences	0	
Sequence length	151	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.17897847222779	33.0	32.0	34.0	16.0	34.0
2	30.357607371137888	33.0	32.0	34.0	16.0	34.0
3	30.425900363584557	33.0	32.0	34.0	16.0	34.0
4	30.528910562539423	33.0	32.0	34.0	16.0	34.0
5	30.258556880202025	33.0	32.0	34.0	16.0	34.0
6	32.37055833133229	37.0	33.0	37.0	16.0	37.0
7	32.35626167898036	37.0	33.0	37.0	16.0	37.0
8	32.420866460633846	37.0	33.0	37.0	16.0	37.0
9	32.34192068129528	37.0	33.0	37.0	16.0	37.0
10-14	32.99741617670577	37.4	33.6	37.4	16.0	37.4
15-19	33.87150477396382	38.0	34.4	38.0	16.0	38.0
20-24	34.07579608128783	38.0	35.0	38.4	16.0	38.4
25-29	34.362065586747725	38.0	35.4	39.0	16.0	39.0
30-34	34.27417370255698	38.0	35.0	39.0	16.0	39.0
35-39	34.218907293250496	38.0	35.2	39.0	16.0	39.0
40-44	34.18040313681903	38.0	35.2	39.0	16.0	39.0
45-49	34.11799783651385	38.0	35.6	39.0	16.0	39.0
50-54	34.10282390137975	38.0	35.6	39.0	16.0	39.0
55-59	34.151466250135044	38.0	35.8	39.0	16.0	39.0
60-64	34.07834603229567	38.0	35.8	39.0	16.0	39.0
65-69	34.001499202491644	38.0	35.8	39.0	16.0	39.0
70-74	33.953751638772346	38.0	35.6	39.0	16.0	39.0
75-79	33.92803299670193	38.0	35.6	39.0	16.0	39.0
80-84	33.93269378556767	38.0	35.6	39.0	16.0	39.0
85-89	33.89797068207368	38.0	35.6	39.0	16.0	39.0
90-94	33.82350000731226	38.0	35.6	39.0	16.0	39.0
95-99	33.74324929578196	38.0	35.4	39.0	16.0	39.0
100-104	33.666762936925366	38.0	34.8	39.0	16.0	39.0
105-109	33.58146596236214	38.0	34.6	39.0	16.0	39.0
110-114	33.39172723657339	38.0	34.0	39.0	16.0	39.0
115-119	33.2941465103761	38.0	34.0	39.0	16.0	39.0
120-124	33.304036982120806	38.0	34.0	39.0	15.4	39.0
125-129	33.13479905667098	38.0	34.0	39.0	15.0	39.0
130-134	32.91735138534349	38.0	34.0	39.0	15.0	39.0
135-139	32.66105691912213	38.0	34.0	39.0	14.0	39.0
140-144	32.26364145005465	38.0	33.2	39.0	13.4	39.0
145-149	31.823597519869303	38.0	33.0	39.0	2.0	39.0
150-151	30.75772988691468	38.0	30.5	39.0	2.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	224.0
1	437.0
2	20393.0
3	8265.0
4	12539.0
5	12422.0
6	10097.0
7	9381.0
8	8777.0
9	8616.0
10	9171.0
11	9672.0
12	10686.0
13	12666.0
14	13870.0
15	16407.0
16	21031.0
17	26905.0
18	35776.0
19	48915.0
20	70490.0
21	86348.0
22	83475.0
23	76595.0
24	72587.0
25	70055.0
26	67525.0
27	65394.0
28	64508.0
29	66119.0
30	67364.0
31	71780.0
32	77447.0
33	87086.0
34	104484.0
35	137597.0
36	210469.0
37	527348.0
38	1936533.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.281490235485325	30.783284151033303	24.87581764099516	25.05940797248622
2	22.741465354624726	30.628082248489584	27.189975366779755	19.44047703010593
3	20.596888745366957	28.82296787056041	27.681834833040565	22.898308551032073
4	21.584295742753625	27.24045610658213	28.147055215881643	23.02819293478261
5	22.750449839265613	27.73767412105606	28.48916753080467	21.022708508873652
6	22.08930881463498	27.575491806604347	27.633048913161424	22.70215046559925
7	22.823772406965286	27.63018750246211	28.119967418772795	21.426072671799805
8	21.53673590462453	27.50371823284571	28.809941392865944	22.14960446966382
9	21.436945539500325	26.78604453234247	29.310638470524076	22.466371457633134
10-14	21.82071510394889	27.73605016830507	28.16917701464466	22.274057713101385
15-19	21.758042455368287	27.472149158946568	28.258982096455725	22.510826289229417
20-24	21.898747482684865	27.39381890918719	28.11925103157671	22.588182576551244
25-29	22.05418043541572	27.363818003836432	27.925917458320104	22.656084102427744
30-34	22.07800025881921	27.320032781250163	27.910419869766667	22.69154709016396
35-39	22.19372402963615	27.288383110316172	27.83925346131556	22.678639398732116
40-44	22.190166093803334	27.384796912458885	27.733298798767986	22.691738194969798
45-49	22.260220133204747	27.262621706447536	27.749942909555525	22.727215250792188
50-54	22.239284552339143	27.260719812333246	27.798131306804148	22.701864328523463
55-59	22.23110327439518	27.272251144621617	27.78599851795476	22.710647063028443
60-64	22.256743295207173	27.231283597571633	27.742605561630796	22.769367545590402
65-69	22.240809438096527	27.20675764917727	27.773802218149928	22.778630694576275
70-74	22.191307054292384	27.207463316333847	27.7993877239588	22.801841905414967
75-79	22.156752188935688	27.250709985661096	27.72729095705454	22.865246868348677
80-84	22.17291690445502	27.307630659812954	27.742172896143437	22.777279539588594
85-89	22.162336989843883	27.32435573847414	27.70770311232358	22.805604159358403
90-94	22.230003186510107	27.23629175524243	27.73824494345572	22.795460114791737
95-99	22.226208347591307	27.264786141254877	27.788282769799576	22.720722741354244
100-104	22.231911031629533	27.357702204015943	27.6487219138285	22.761664850526024
105-109	22.253624118732574	27.24929059095787	27.669252817146926	22.827832473162633
110-114	22.213955357230482	27.3052663384791	27.723438435389298	22.75733986890112
115-119	22.274576867876117	27.393825799244848	27.604350982747295	22.72724635013174
120-124	22.31461665525673	27.336350726393103	27.576787293981354	22.77224532436881
125-129	22.327737128064243	27.281429919644346	27.585455137668575	22.805377814622837
130-134	22.235517026100514	27.413550632137728	27.61292079434236	22.7380115474194
135-139	22.267294455112026	27.369638754299142	27.533315090983717	22.829751699605115
140-144	22.302188075219824	27.355030804677185	27.586833747594614	22.75594737250838
145-149	22.31436276517246	27.39180321990749	27.55751182790423	22.73632218701582
150-151	22.368833554718766	27.29750781571957	27.497698275599742	22.835960353961926
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	44.34089820797154
2	42.18194238473066
3	43.49519729639903
4	44.612488677536234
5	43.77315834813926
6	44.79145928023423
7	44.24984507876509
8	43.68634037428835
9	43.90331699713346
10-14	44.09477281705027
15-19	44.268868744597704
20-24	44.48693005923611
25-29	44.710264537843464
30-34	44.76954734898317
35-39	44.87236342836827
40-44	44.88190428877313
45-49	44.98743538399694
50-54	44.941148880862606
55-59	44.94175033742363
60-64	45.02611084079757
65-69	45.0194401326728
70-74	44.993148959707355
75-79	45.02199905728437
80-84	44.95019644404361
85-89	44.96794114920228
90-94	45.02546330130184
95-99	44.94693108894555
100-104	44.99357588215556
105-109	45.08145659189521
110-114	44.9712952261316
115-119	45.00182321800785
120-124	45.08686197962554
125-129	45.13311494268708
130-134	44.973528573519914
135-139	45.09704615471714
140-144	45.058135447728205
145-149	45.05068495218828
150-151	45.20479390868069
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	370.0
1	321.5
2	252.5
3	237.5
4	275.5
5	332.5
6	390.0
7	460.5
8	537.5
9	608.0
10	678.0
11	790.5
12	890.5
13	1027.5
14	1195.0
15	1332.5
16	1538.0
17	1822.5
18	2110.5
19	2371.5
20	2803.0
21	3404.0
22	3988.5
23	4773.0
24	5909.0
25	7088.5
26	8404.5
27	10279.5
28	12748.0
29	15140.5
30	17777.0
31	21062.0
32	24681.5
33	29372.0
34	39465.5
35	49888.0
36	61987.0
37	83919.5
38	111722.5
39	133600.5
40	156929.0
41	197312.0
42	232091.0
43	255278.0
44	282475.0
45	305028.5
46	313273.0
47	314998.5
48	292753.5
49	242777.0
50	198990.5
51	164624.5
52	132072.5
53	111597.5
54	96164.5
55	74517.5
56	54626.5
57	39341.5
58	28850.5
59	21714.5
60	15969.5
61	11621.0
62	8332.5
63	5891.0
64	4238.5
65	3050.5
66	2138.0
67	1514.5
68	1160.5
69	902.5
70	666.5
71	469.5
72	364.5
73	273.0
74	207.5
75	170.0
76	124.5
77	96.5
78	85.0
79	67.0
80	46.0
81	40.0
82	33.0
83	23.0
84	21.0
85	22.0
86	16.5
87	20.5
88	27.0
89	21.0
90	18.0
91	23.0
92	27.5
93	24.0
94	22.0
95	20.5
96	25.0
97	34.0
98	44.5
99	64.0
100	394.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002924904952383019
2	0.0013445127603696136
3	0.0016747439646709224
4	0.0022172666574516438
5	0.003821246792629428
6	0.004387357428574529
7	0.004788352462368975
8	0.00492988012135525
9	0.004670412746547079
10-14	0.004429815726270411
15-19	0.006656517560987806
20-24	0.0050761253689744
25-29	0.00481665799416623
30-34	0.004750611753305968
35-39	0.004689283101078582
40-44	0.005260111325656559
45-49	0.013350775831038619
50-54	0.020686626155160544
55-59	0.016912555248859876
60-64	0.01780417950047341
65-69	0.024932455924748798
70-74	0.022965221464839577
75-79	0.015001931852545164
80-84	0.017469230707539227
85-89	0.012525197820285348
90-94	0.007868937839636898
95-99	0.007203757842401404
100-104	0.010105074851620044
105-109	0.015195352986493072
110-114	0.013614960794479668
115-119	0.005807351607070156
120-124	0.0039014457993883176
125-129	0.004727023810141589
130-134	0.004807222816900479
135-139	0.00551957870046473
140-144	0.009963547192633768
145-149	0.007689669471587615
150-151	0.005684694302615384
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	4239454.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	58.45845136921624
#Duplication Level	Relative count
1	100.0
2	17.135023015286695
3	7.830880263681309
4	4.196027731999773
5	2.281638915724271
6	1.25021310450645
7	0.6982724328010457
8	0.4304711030289254
9	0.236546002159459
10++	8.010598397454112
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	4101485	3.9962406	5.900525	7
>>END_MODULE