The 1,000 Soils Pilot is composed of crowdsourced soil cores sampled and analyzed using standardized materials and protocols. The data types include molecular and microstructural data that describe soil structure, soil organic matter (SOM) chemistry, and soil microbiology.The  Environmental Molecular Sciences Laboratory (EMSL) analyzed samples using Fourier-transform ion cyclotron resonance-(FTICR), liquid chromatography-(LC) mass spectrometry (MS), X-ray Computed Tomography (XCT), and water retention curves while outsourced vendors provided metagenomic sequencing and standard biogeochemical measurements. Data and analytic workflows from the 1000 Soils Pilot will populate a unique continental-scale database of soil molecular properties, as part of the EMSL Molecular Observation Network (MONet) user program.
The U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research (BER) under contract DE-AC05-76RL01830 (PNNL) for the Environmental Molecular Sciences Laboratory.