Hypersaline microbial communities from Antarctic Deep Lake - 36m 3.0um, 0.8um, 0.1um pool
DNA was extracted by exposing filter to lysozyme, proteinase K, 10% SDS, freeze-thawing (3 cycles at 80deg C and 55 deg C), phenol-extracted twice and phenol-chloroform extracted once. It was then precipitated using isopropanol and re-solubilized in TE
Deep Lake Antarctica
Material from 3.0, 0.8 and 0.1 um filters was pooled for library construction
Deep Lake Antarctica