Metatranscriptomic Assembly Workflow - Info File This workflow assembles metatranscriptomic reads using a workflow developed by Brian Foster at JGI. The reads are assembled using SPAdes(1): System information: SPAdes version: 3.15.2 Python version: 3.9.16 OS: Linux-5.14.21-150400.24.81_12.0.87-cray_shasta_c-x86_64-with-glibc2.34 An AGP file is created using fungalrelease.sh (BBTools(2) Version 38.96). Assembled reads are mapped using bbmap.sh (BBTools(2) Version 38.96). The following are the Docker images used in this workflow: microbiomedata/bbtools:38.96 bryce911/spades:3.15.2 (1) Bankevich, A., Nurk, S., Antipov, D., Gurevich, A. A., Dvorkin, M., Kulikov, A. S., Lesin, V. M., Nikolenko, S. I., Pham, S., Prjibelski, A. D., Pyshkin, A. V., Sirotkin, A. V., Vyahhi, N., Tesler, G., Alekseyev, M. A., & Pevzner, P. A. (2012). Spades: A new genome assembly algorithm and its applications to single-cell sequencing. Journal of Computational Biology, 19(5), 455-477. https://doi.org/10.1089/cmb.2012.0021 (2) B. Bushnell: BBTools software package, http://bbtools.jgi.doe.gov/